FastQCFastQC Report
Fri 27 May 2016
SRR1233072_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233072_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75016355
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGAATTGTAATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTT2375530.3166682785373936No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG1807730.24097811737187175No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1678590.2237632047038276No Hit
GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT1411320.18813497403332913No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1309190.17452060953907983TruSeq Adapter, Index 6 (100% over 50bp)
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA1290620.1720451493544308No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA1178230.1570630831103431No Hit
CGGAAAATGAGAAATACACACTTTAGGACGTGAAATATGGCGAGGAAAAC1010000.13463730675797297No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890530.1187114463239383No Hit
CCGGAGAATTGTAATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTT846290.11281406567941084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAGA421250.035.429291
CGGACGT66400.034.0231861
CGCGTGC86200.032.718761
CGGCGAG149250.032.5994831
CGGCAAG186050.032.440041
GGAGAAT1415150.030.9700761
GAATTGT1470000.030.5252824
AATTGTA1561900.029.9943125
GAGAATT1488500.029.034182
AGAATTG1520900.027.9305553
ATTGTAA1778000.027.8554866
CGGAGAA542550.027.3168932
TGCCGTC398400.027.00025748-49
CGCGTGT2785600.026.4166151
TTGTAAT1887100.026.0794877
GTAATAC1890250.026.038549
TATGCCG419650.025.94276646-47
CGTATGC421750.025.82993144-45
CGGAGAT254800.025.6843411
CCGTCTT422800.025.55552150-51
Produced by FastQC (version 0.11.5)