##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233072_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 75016355 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.988505786504824 33.0 31.0 34.0 30.0 34.0 2 32.23823648856306 34.0 31.0 34.0 30.0 34.0 3 30.721683038318776 33.0 31.0 34.0 25.0 34.0 4 34.20665038977167 37.0 35.0 37.0 28.0 37.0 5 35.17609506620256 37.0 35.0 37.0 32.0 37.0 6 35.61055685790119 37.0 35.0 37.0 33.0 37.0 7 35.84006073075665 37.0 35.0 37.0 35.0 37.0 8 35.92160180270022 37.0 35.0 37.0 35.0 37.0 9 37.692391932399275 39.0 37.0 39.0 35.0 39.0 10-11 37.658537774862566 39.0 37.0 39.0 35.0 39.0 12-13 37.631595690033194 39.0 37.0 39.0 35.0 39.0 14-15 39.025518901844805 40.0 38.0 41.0 36.0 41.0 16-17 38.90476159498819 40.0 38.0 41.0 35.0 41.0 18-19 38.809212497728 40.0 38.0 41.0 34.5 41.0 20-21 38.731473470018635 40.0 38.0 41.0 34.0 41.0 22-23 38.56384794355844 40.0 38.0 41.0 34.0 41.0 24-25 38.39324653937132 40.0 38.0 41.0 34.0 41.0 26-27 38.072442262757235 40.0 38.0 41.0 33.5 41.0 28-29 37.97545482581765 40.0 38.0 41.0 33.0 41.0 30-31 37.823538033806095 40.0 38.0 41.0 33.0 41.0 32-33 37.71712157302231 40.0 38.0 41.0 33.0 41.0 34-35 37.629374321106376 40.0 38.0 41.0 33.0 41.0 36-37 37.52017889965461 40.0 38.0 41.0 32.5 41.0 38-39 37.397581180797175 40.0 38.0 41.0 32.0 41.0 40-41 37.31659763394262 40.0 37.5 41.0 32.0 41.0 42-43 37.21911918407659 40.0 37.0 41.0 32.0 41.0 44-45 37.23161193982299 40.0 37.0 41.0 32.0 41.0 46-47 37.17494833626614 40.0 37.0 41.0 32.0 41.0 48-49 37.06255527877887 40.0 37.0 41.0 31.0 41.0 50-51 36.95987417010598 40.0 37.0 41.0 31.0 41.0 52-53 36.81186228656404 40.0 36.5 41.0 31.0 41.0 54-55 36.65131038424887 40.0 36.0 41.0 31.0 41.0 56-57 36.45216659087208 40.0 36.0 41.0 30.0 41.0 58-59 36.234674318686906 39.5 35.5 41.0 29.5 41.0 60-61 35.93412313888085 39.0 35.0 41.0 29.0 41.0 62-63 35.680163965577904 39.0 35.0 41.0 28.0 41.0 64-65 35.41758641832171 38.5 35.0 40.0 28.0 41.0 66-67 35.11337039103006 38.0 35.0 40.0 28.0 41.0 68-69 34.73978819018866 37.0 34.5 40.0 27.5 41.0 70-71 34.314572942127086 37.0 34.0 39.5 26.0 41.0 72-73 33.937198928153734 36.0 34.0 39.0 26.0 41.0 74-75 33.511469392241196 36.0 34.0 39.0 26.0 40.5 76-77 32.3058580492214 35.0 31.5 37.0 24.5 39.0 78-79 32.705367709481486 35.0 33.0 37.0 25.0 39.0 80-81 32.520308704681796 35.0 33.0 37.0 25.0 39.0 82-83 32.167945083442135 35.0 33.0 36.5 24.5 39.0 84-85 31.831030240005664 35.0 33.0 36.0 23.5 37.0 86-87 31.53091956414038 35.0 33.0 36.0 22.5 37.0 88-89 31.202683241541127 35.0 32.0 35.0 20.0 37.0 90-91 30.923427237966976 35.0 32.0 35.0 19.5 36.0 92-93 30.683826826829964 35.0 32.0 35.0 18.0 36.0 94-95 30.443860755431267 35.0 32.0 35.0 10.0 36.0 96-97 30.181868240599 34.5 32.0 35.0 2.0 36.0 98-99 29.852766220112933 34.0 31.5 35.0 2.0 35.0 100-101 28.535257771455306 33.5 29.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5215713419224954 1101 2 0.4135687611243313 1101 3 1.951508459828684 1101 4 1.920011913282984 1101 5 0.9369181210935906 1101 6 0.5639020452382226 1101 7 0.34981644643299603 1101 8 0.26055728401660616 1101 9 0.27061139215781793 1101 10-11 0.2892680907867273 1101 12-13 0.2726074923313533 1101 14-15 0.3161735883827177 1101 16-17 0.3099475801189584 1101 18-19 0.3309682010277939 1101 20-21 0.3226786816067886 1101 22-23 0.31206037350458615 1101 24-25 0.2762442723568199 1101 26-27 0.31422352550998056 1101 28-29 0.25188529222394607 1101 30-31 0.22379512705499138 1101 32-33 0.22891453959127972 1101 34-35 0.24014193330361877 1101 36-37 0.1390675833469004 1101 38-39 0.10754258939186201 1101 40-41 0.09257201000818327 1101 42-43 0.05210153117665328 1101 44-45 0.19122143411558312 1101 46-47 0.2943990132842984 1101 48-49 0.2784406334873708 1101 50-51 0.24441579284938797 1101 52-53 0.1596536492305276 1101 54-55 0.15370256466915322 1101 56-57 0.1910376479369731 1101 58-59 0.12779940274089796 1101 60-61 0.08076056857358083 1101 62-63 0.12479818282356092 1101 64-65 0.03080684333810524 1101 66-67 0.01959803876461308 1101 68-69 0.020414503702369302 1101 70-71 0.11851754252401037 1101 72-73 0.024372195633226568 1101 74-75 -0.010424533357131338 1101 76-77 0.013401595817597922 1101 78-79 0.0379214048235923 1101 80-81 0.08965061936816454 1101 82-83 0.04216556405834382 1101 84-85 0.014635133889278507 1101 86-87 -0.027319339834487977 1101 88-89 -0.0400741872539605 1101 90-91 -0.08324035318974765 1101 92-93 -0.12672728111746068 1101 94-95 -0.21939383805146662 1101 96-97 -0.2908096511409255 1101 98-99 -0.35070613862961864 1101 100-101 -0.44766422304376974 1102 1 0.5119163780677667 1102 2 0.4037650878333423 1102 3 1.9186258524700186 1102 4 1.929159624785754 1102 5 0.9648573914458396 1102 6 0.5390646907654713 1102 7 0.3558320807873301 1102 8 0.27088947061564284 1102 9 0.3017514960219785 1102 10-11 0.2895650248982804 1102 12-13 0.26661911245677317 1102 14-15 0.30925776281286943 1102 16-17 0.2990148764575338 1102 18-19 0.22403770765709652 1102 20-21 0.2741563814541621 1102 22-23 0.2885069840830212 1102 24-25 0.3050423815299723 1102 26-27 0.26585052767403283 1102 28-29 0.2270567774054726 1102 30-31 0.2195665896355976 1102 32-33 0.25757676087501835 1102 34-35 0.23215662428367523 1102 36-37 0.1613539776505064 1102 38-39 0.13878279588789866 1102 40-41 0.15871720549696988 1102 42-43 0.13707778718860908 1102 44-45 0.24089670015549558 1102 46-47 0.25455542423730293 1102 48-49 0.2946762886210692 1102 50-51 0.31319935116327713 1102 52-53 0.2268514095041141 1102 54-55 0.17761575992295064 1102 56-57 0.16391046615958516 1102 58-59 0.20089802043876404 1102 60-61 0.19429261824891597 1102 62-63 0.17830595814681516 1102 64-65 0.16895471147094554 1102 66-67 0.14785302718286175 1102 68-69 0.20240085620608994 1102 70-71 0.1542127223474523 1102 72-73 0.1260687796756983 1102 74-75 0.10353301259115 1102 76-77 0.10011462499259238 1102 78-79 0.15630733661001273 1102 80-81 0.2165056056804744 1102 82-83 0.18344483042046988 1102 84-85 0.1377666405121296 1102 86-87 0.13574454196357877 1102 88-89 0.14132335148456932 1102 90-91 0.07372252860928441 1102 92-93 0.06187591542741444 1102 94-95 0.06732314174774245 1102 96-97 0.012690174356293937 1102 98-99 -0.02050957853485258 1102 100-101 -0.0932380296957227 1103 1 0.5241003471578409 1103 2 0.4223329460056391 1103 3 1.9339237431388945 1103 4 1.9171113912297741 1103 5 0.95374174108332 1103 6 0.5244020540202143 1103 7 0.35158159004731715 1103 8 0.2696953744771804 1103 9 0.27853931210466243 1103 10-11 0.2754904659015196 1103 12-13 0.2603226100691529 1103 14-15 0.3351916870488978 1103 16-17 0.3281314708807983 1103 18-19 0.25296624769332965 1103 20-21 0.2550188885010698 1103 22-23 0.3051862164759669 1103 24-25 0.29778990420079765 1103 26-27 0.2832556835105038 1103 28-29 0.26880357780589037 1103 30-31 0.29121870527620786 1103 32-33 0.2756715190618806 1103 34-35 0.26886027144128377 1103 36-37 0.24598694764787865 1103 38-39 0.15127128139587143 1103 40-41 0.22145552736795793 1103 42-43 0.17044562889378767 1103 44-45 0.3010151196225408 1103 46-47 0.30626769052685177 1103 48-49 0.3729147271553117 1103 50-51 0.3743697663192336 1103 52-53 0.23649636316914524 1103 54-55 0.23326267462859818 1103 56-57 0.2640467819207757 1103 58-59 0.21686949835977742 1103 60-61 0.2533500795208212 1103 62-63 0.28016629687822103 1103 64-65 0.229129338240476 1103 66-67 0.25368447022373175 1103 68-69 0.21127899970910846 1103 70-71 0.18332014449383394 1103 72-73 0.17552680189826475 1103 74-75 0.17834989343121066 1103 76-77 0.1314272759355859 1103 78-79 0.16624982394307608 1103 80-81 0.1858187758192642 1103 82-83 0.1273080961045494 1103 84-85 0.09661597510470799 1103 86-87 0.18035350911845782 1103 88-89 0.16994046756764547 1103 90-91 0.173451850713505 1103 92-93 0.047797036962247574 1103 94-95 0.032364142240560057 1103 96-97 0.01399668265769094 1103 98-99 -0.08444772579851545 1103 100-101 -0.11632848143021235 1104 1 0.44908690211268976 1104 2 0.3650498971090599 1104 3 1.8574559546686267 1104 4 1.8575124152547318 1104 5 0.8717373411084566 1104 6 0.46659965635740264 1104 7 0.2598207535078032 1104 8 0.21679580182389913 1104 9 0.2242739224781971 1104 10-11 0.20998369633171876 1104 12-13 0.19952674105967816 1104 14-15 0.20015705245855742 1104 16-17 0.21722518048669315 1104 18-19 0.20447788860885652 1104 20-21 0.173681965457817 1104 22-23 0.19169812118973795 1104 24-25 0.16929003651711128 1104 26-27 0.18184583116627095 1104 28-29 0.14292787035646626 1104 30-31 0.19570010297553608 1104 32-33 0.17341423253418498 1104 34-35 0.14845967392223258 1104 36-37 0.1421356394172264 1104 38-39 0.11092810363866334 1104 40-41 0.08925310862696989 1104 42-43 0.07269197998972743 1104 44-45 0.19544641427047083 1104 46-47 0.18926276520196694 1104 48-49 0.19533258850385948 1104 50-51 0.20941471535643075 1104 52-53 0.18756124797179297 1104 54-55 0.15006619224649853 1104 56-57 0.124826075286812 1104 58-59 0.16166064653614143 1104 60-61 0.1442033394091311 1104 62-63 0.15103417991791446 1104 64-65 0.16511331753610392 1104 66-67 0.2061402569012003 1104 68-69 0.13107544471016297 1104 70-71 0.1884340273477818 1104 72-73 0.197093536850808 1104 74-75 0.19292036058322282 1104 76-77 0.14915306203347 1104 78-79 0.1705659770793062 1104 80-81 0.22487231075093206 1104 82-83 0.16424763637290596 1104 84-85 0.10211423637908368 1104 86-87 0.13706470954869943 1104 88-89 0.1462419620584683 1104 90-91 0.10628870260824996 1104 92-93 0.08838757851820489 1104 94-95 0.07633610382563205 1104 96-97 0.04270329659295413 1104 98-99 -0.016688092012081768 1104 100-101 -0.11794322990822081 1105 1 0.41631205555621165 1105 2 0.3558429951576727 1105 3 1.8510776323740892 1105 4 1.8297554042275834 1105 5 0.8794252188793763 1105 6 0.46818033062481135 1105 7 0.3005237108157033 1105 8 0.22330942812234866 1105 9 0.18778155417746234 1105 10-11 0.14722674603763153 1105 12-13 0.15199157024557763 1105 14-15 0.1721388633652623 1105 16-17 0.16129552496710886 1105 18-19 0.122839542142259 1105 20-21 0.13334056488778145 1105 22-23 0.2175636170536137 1105 24-25 0.194443132604043 1105 26-27 0.19603344847684667 1105 28-29 0.18660745285870206 1105 30-31 0.22708271431912408 1105 32-33 0.21806344727054494 1105 34-35 0.1954447571559612 1105 36-37 0.2271887525746834 1105 38-39 0.25701747958480325 1105 40-41 0.19635521784914545 1105 42-43 0.19718607855536163 1105 44-45 0.2628284779320822 1105 46-47 0.2790210461568208 1105 48-49 0.2781861047663128 1105 50-51 0.2974513395076528 1105 52-53 0.32071547335022643 1105 54-55 0.2999437726424645 1105 56-57 0.3135591373675837 1105 58-59 0.29561970050297504 1105 60-61 0.38876398875874685 1105 62-63 0.3573899625653567 1105 64-65 0.30032468356604625 1105 66-67 0.33351331865915057 1105 68-69 0.3460390532857147 1105 70-71 0.34031200046717913 1105 72-73 0.3488124855686294 1105 74-75 0.35964449205921056 1105 76-77 0.3039107878919225 1105 78-79 0.33214237856454076 1105 80-81 0.36820049345190853 1105 82-83 0.33776667165972185 1105 84-85 0.31536263455788216 1105 86-87 0.36185134784376416 1105 88-89 0.39599586441998724 1105 90-91 0.4046205163063341 1105 92-93 0.35849846766327786 1105 94-95 0.30141794973619795 1105 96-97 0.2275480254194946 1105 98-99 0.15473828321872318 1105 100-101 0.09501606710642463 1106 1 0.35388858310371063 1106 2 0.3063920879003277 1106 3 1.7930688474732541 1106 4 1.8245779499116495 1106 5 0.8330624569925433 1106 6 0.41712569980813896 1106 7 0.2698060889194309 1106 8 0.16812409913334392 1106 9 0.1486818709263602 1106 10-11 0.14799393880387157 1106 12-13 0.10288954381788784 1106 14-15 0.13382127835330948 1106 16-17 0.11202466750961548 1106 18-19 0.11359108652406036 1106 20-21 0.10053425533826754 1106 22-23 0.1296548277920806 1106 24-25 0.0735630275529644 1106 26-27 0.11291185133891446 1106 28-29 0.1411715091388288 1106 30-31 0.15478102922660497 1106 32-33 0.147010352320585 1106 34-35 0.11096321356500027 1106 36-37 0.14212001735060653 1106 38-39 0.1277828210605989 1106 40-41 0.16615614988529614 1106 42-43 0.13427544161976357 1106 44-45 0.13987235173421197 1106 46-47 0.09399709499032127 1106 48-49 0.14117164124827042 1106 50-51 0.10776897432822352 1106 52-53 0.13743473379921767 1106 54-55 0.16132553927234738 1106 56-57 0.16981505343215275 1106 58-59 0.10804450120119213 1106 60-61 0.1751798126651778 1106 62-63 0.14049120972865126 1106 64-65 0.1843170156640781 1106 66-67 0.15192305636171 1106 68-69 0.16087544175115198 1106 70-71 0.1370221078075815 1106 72-73 0.11931868804642676 1106 74-75 0.16959438729539755 1106 76-77 0.11592550172710503 1106 78-79 0.13539557900008248 1106 80-81 0.10745968947782814 1106 82-83 0.14173813451697015 1106 84-85 0.09201907030213619 1106 86-87 0.180812235605881 1106 88-89 0.16351003080744775 1106 90-91 0.1600702232078106 1106 92-93 0.13838576288468474 1106 94-95 0.07829932410984242 1106 96-97 -0.023614514520282626 1106 98-99 -0.1012376890172959 1106 100-101 -0.17201463218374258 1107 1 0.32249311350965115 1107 2 0.273957871549527 1107 3 1.7304587653142924 1107 4 1.7530637182764295 1107 5 0.7737668107886435 1107 6 0.37847235954330927 1107 7 0.23839290759309506 1107 8 0.1491239282977901 1107 9 0.12052708434674742 1107 10-11 0.04473671914598043 1107 12-13 0.0424230840702009 1107 14-15 0.004387856467161555 1107 16-17 0.020703959809509342 1107 18-19 0.03227773842756676 1107 20-21 -0.022291728102430852 1107 22-23 -0.04911228372504439 1107 24-25 -0.024383153763452015 1107 26-27 -0.023627944925884492 1107 28-29 0.01734030414824872 1107 30-31 -0.023628591001099153 1107 32-33 -0.06233299700667061 1107 34-35 -0.05074644135450512 1107 36-37 -0.1132014358440756 1107 38-39 -0.0025632602650276226 1107 40-41 -0.06347200338171177 1107 42-43 -0.0983676103071005 1107 44-45 -0.0014666607092834738 1107 46-47 -0.055448447977504145 1107 48-49 0.013284404398810068 1107 50-51 0.005263708990931093 1107 52-53 -0.02336374718874623 1107 54-55 0.04130640459278112 1107 56-57 -0.00910729305906699 1107 58-59 -0.04631753707874253 1107 60-61 0.011212684389370509 1107 62-63 0.030027531608773472 1107 64-65 0.006267725125326251 1107 66-67 0.011585614886584494 1107 68-69 -0.04332653182388668 1107 70-71 -0.1195470169937991 1107 72-73 -0.10997503557810973 1107 74-75 -0.03604599930334018 1107 76-77 -0.07497877577085887 1107 78-79 -0.004043726108420742 1107 80-81 0.01416714661110774 1107 82-83 0.023123924533486218 1107 84-85 -0.06670246905748556 1107 86-87 0.02027797917950025 1107 88-89 -0.040038746460044905 1107 90-91 0.02171433868310757 1107 92-93 0.005946302768162326 1107 94-95 0.037026752287040665 1107 96-97 -0.007708406479061125 1107 98-99 -0.14790230709298768 1107 100-101 -0.28905220407813914 1108 1 0.25776867971417516 1108 2 0.2034634985390653 1108 3 1.6844939824195144 1108 4 1.7394327584942886 1108 5 0.7643046609162027 1108 6 0.30994583274680565 1108 7 0.16828628315904837 1108 8 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-1.0139381954535445 2316 88-89 -0.9840484789870665 2316 90-91 -0.9912219272432345 2316 92-93 -1.0281276668789907 2316 94-95 -1.1288624869433193 2316 96-97 -1.1878833599951335 2316 98-99 -1.376396569747829 2316 100-101 -1.219005095451955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6930.0 3 845.0 4 2270.0 5 5787.0 6 15814.0 7 49134.0 8 150038.0 9 317768.0 10 480266.0 11 439820.0 12 313075.0 13 224226.0 14 178191.0 15 167360.0 16 176451.0 17 194087.0 18 215868.0 19 242060.0 20 277899.0 21 322605.0 22 379109.0 23 416155.0 24 437188.0 25 487836.0 26 576237.0 27 708085.0 28 824485.0 29 1018956.0 30 1268369.0 31 1585188.0 32 1990257.0 33 2554894.0 34 3403556.0 35 4939811.0 36 7579408.0 37 1.4286933E7 38 2.2077789E7 39 6689182.0 40 12423.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.79329478569658 14.305925122401087 17.75253685275019 35.148243239152144 2 24.216861522108204 21.238014521432568 39.114715668818356 15.430408287640871 3 24.14210769903829 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22-23 19.356085083046224 30.257346547962776 34.195990327709204 16.190578041281796 24-25 19.249012572967057 30.29523815839897 33.633644500140484 16.82210476849349 26-27 20.79798772533302 30.51887147032206 30.740664070475926 17.942476733868993 28-29 20.90385132902503 30.82607424184078 29.46686131406322 18.80321311507097 30-31 20.2457666602457 29.78470272140175 31.578256380586993 18.391274237765558 32-33 20.247404090817724 30.740458552005993 30.59438139055921 18.41775596661707 34-35 17.92442689393092 31.915655199440724 29.92595672981317 20.23396117681519 36-37 18.568373316151632 29.815248496869973 31.960486041791775 19.65589214518662 38-39 19.902958050301756 28.222633329532222 33.024312305022455 18.850096315143567 40-41 20.164495655038444 29.397374915351087 31.5410904437827 18.89703898582777 42-43 20.05813793445168 30.772466639519596 30.88468657806851 18.284708847960214 44-45 19.58741292124118 30.17588925137179 31.531238680926126 18.705459146460896 46-47 18.867266811406918 30.35189309060237 31.813320098126148 18.96751999986456 48-49 19.60740441934639 30.236307550134605 30.74401044851585 19.412277582003153 50-51 19.72838309210457 29.999553883040207 31.301640179363684 18.97042284549154 52-53 18.928966935400553 30.983040770007563 32.27481311385807 17.813179180733812 54-55 18.840131143124438 31.386109964334945 31.575270315563287 18.198488576977326 56-57 19.848163574085607 29.30680902623409 31.322873339267222 19.522154060413083 58-59 20.02842961737395 29.14088970883631 30.959989561418546 19.8706911123712 60-61 21.540759589326754 29.36165161911474 30.277403360089227 18.82018543146928 62-63 18.258563743102417 33.49715527802747 30.005221847815612 18.239059131054503 64-65 17.529313332085664 32.541251610170164 31.07197537460281 18.85745968314136 66-67 18.864810446109203 29.182856868128866 32.66335623025449 19.288976455507438 68-69 20.2931588635655 31.66261949213864 29.05095984629033 18.993261798005534 70-71 19.09001404995135 33.630847534649156 28.77083451629354 18.50830389910595 72-73 18.23290506805372 32.285694032472875 30.833892364534176 18.64750853493922 74-75 19.217575801622885 30.27274408609398 31.389452782864986 19.120227329418153 76-77 19.71350520619683 30.619702135571686 29.7209751187233 19.94581753950818 78-79 19.17953566675744 32.103510955152615 29.13977055078984 19.577182827300106 80-81 18.869969596164797 31.622893318208067 31.823288715500546 17.68384837012659 82-83 19.035454220333403 31.68961419516662 31.125944026712364 18.148987557787606 84-85 19.242387210858038 31.65514606855736 29.971381316084074 19.13108540450053 86-87 19.710148084337824 32.03081474046456 29.08277701650625 19.176260158691367 88-89 19.022543567095752 31.411183963933205 30.73626792652202 18.83000454244902 90-91 18.777532418990425 32.16991369637462 29.984279534358333 19.06827435027662 92-93 17.97167495254606 32.8578156906585 29.680170356548118 19.49033900024732 94-95 17.71026166160656 32.420433932555135 29.978314616896306 19.890989788941997 96-97 18.864443119131614 30.684423965681784 30.92858972651139 19.52254318867521 98-99 19.610516817363457 31.45912128145684 29.417849774982923 19.512512126196775 100-101 19.855453291207386 32.28369332037345 28.42413938608504 19.436714002334117 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 80497.0 1 70607.0 2 50061.5 3 43041.0 4 50543.5 5 60244.5 6 63068.0 7 57802.0 8 56208.5 9 58827.0 10 60763.0 11 63389.5 12 73207.0 13 83206.0 14 102345.0 15 121323.0 16 126587.5 17 144076.5 18 196618.0 19 228730.5 20 236952.5 21 261498.0 22 293476.5 23 343090.0 24 408371.5 25 491462.5 26 591037.5 27 715145.5 28 871814.0 29 1070122.0 30 1334350.5 31 1703454.0 32 2247526.5 33 2987099.0 34 3868974.0 35 4858151.5 36 5753356.5 37 6035586.5 38 5461945.0 39 4433428.5 40 3463886.0 41 2773293.0 42 2352327.5 43 2109319.5 44 1945319.0 45 1809579.0 46 1686597.5 47 1572202.0 48 1461782.0 49 1350126.0 50 1238717.5 51 1117208.5 52 992983.0 53 884506.5 54 790054.5 55 701741.5 56 616149.5 57 532657.5 58 448145.5 59 358096.5 60 271375.5 61 202669.5 62 152963.5 63 115124.0 64 85816.0 65 64476.5 66 49372.0 67 37971.5 68 27968.5 69 20370.5 70 15223.5 71 11695.5 72 9456.5 73 7911.0 74 6119.5 75 4203.5 76 3062.5 77 2414.5 78 1818.5 79 1230.5 80 861.5 81 628.5 82 409.0 83 256.5 84 170.5 85 116.0 86 84.0 87 58.5 88 35.0 89 20.0 90 15.0 91 10.5 92 5.0 93 2.0 94 1.0 95 2.5 96 3.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09048426839720485 2 0.03496970760576144 3 0.002491456696343084 4 0.0 5 0.0 6 0.006406602933453645 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.3326066029201235E-6 18-19 0.0 20-21 8.358177360123668E-4 22-23 0.0 24-25 6.998473866132259E-5 26-27 7.358395379247632E-4 28-29 4.898931706292582E-4 30-31 0.002272837703191524 32-33 0.002064216529848724 34-35 0.0023628180814703674 36-37 0.004288398176637615 38-39 0.004304394688331631 40-41 0.001818936683873803 42-43 3.652536836800455E-4 44-45 0.002580770553301343 46-47 0.0017122932725803593 48-49 7.111782490631542E-4 50-51 0.003191970604276894 52-53 0.005472140041994843 54-55 0.010359740885837495 56-57 0.011634796172114735 58-59 0.0031073223965627232 60-61 8.11822968471342E-4 62-63 0.0010184445778523895 64-65 0.002356819389585111 66-67 0.0026334257376274812 68-69 8.611455461945599E-4 70-71 0.0020055626536373303 72-73 0.002542778838028054 74-75 3.7725106745055794E-4 76-77 0.001001115023517205 78-79 1.3597034939914104E-4 80-81 2.1461986522805594E-4 82-83 0.010598355518606576 84-85 6.685208845457767E-4 86-87 6.531908941723442E-5 88-89 8.711433660033202E-4 90-91 0.002292166821488461 92-93 0.0013090478736270245 94-95 0.0038971501614547925 96-97 1.3863643468147712E-4 98-99 6.398604677606636E-5 100-101 0.002372149379958544 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7.5016355E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.90784436143922 #Duplication Level Percentage of deduplicated Percentage of total 1 82.52182541718842 44.48573721011652 2 9.649964696824377 10.40417589939583 3 3.1470263158577314 5.089482145098362 4 1.4245433714746296 3.0717624942229684 5 0.8048352469336908 2.169346661415095 6 0.5134653844548888 1.6607887218108415 7 0.35063911941396214 1.3231539353479977 8 0.25218074100315957 1.0875616109560593 9 0.18703313334248683 0.9074297738393179 >10 1.0082878274257472 9.906410163982152 >50 0.08008187231482072 3.008074301032855 >100 0.0522640488620811 5.40669780493957 >500 0.004277623293977106 1.5894843259211553 >1k 0.0029185182412322127 3.0521254617339793 >5k 2.985118691988293E-4 1.1153797935387126 >10k+ 3.581714995212401E-4 5.722389696648633 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGAGAATTGTAATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTT 237553 0.3166682785373936 No Hit GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG 180773 0.24097811737187175 No Hit CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 167859 0.2237632047038276 No Hit GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT 141132 0.18813497403332913 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC 130919 0.17452060953907983 TruSeq Adapter, Index 6 (100% over 50bp) AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA 129062 0.1720451493544308 No Hit AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA 117823 0.1570630831103431 No Hit CGGAAAATGAGAAATACACACTTTAGGACGTGAAATATGGCGAGGAAAAC 101000 0.13463730675797297 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 89053 0.1187114463239383 No Hit CCGGAGAATTGTAATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTT 84629 0.11281406567941084 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.07586745583679719 6.665213205840246E-6 0.0 6.665213205840246E-6 0.0 2 0.07609673917107809 6.665213205840246E-6 1.3330426411680492E-6 1.1997383770512443E-5 0.0 3 0.07640600506382908 6.665213205840246E-6 1.3330426411680492E-6 1.1997383770512443E-5 1.3330426411680492E-6 4 0.07737379402131708 7.998255847008296E-6 1.3330426411680492E-6 1.5996511694016593E-5 1.3330426411680492E-6 5 0.0778776841396786 7.998255847008296E-6 2.6660852823360984E-6 1.732955433518464E-5 2.6660852823360984E-6 6 0.07840823511086349 7.998255847008296E-6 2.6660852823360984E-6 2.7993895464529036E-5 2.6660852823360984E-6 7 0.07876415749605536 7.998255847008296E-6 2.6660852823360984E-6 3.465910867036928E-5 3.999127923504148E-6 8 0.07918939809858797 1.0664341129344394E-5 2.6660852823360984E-6 4.5323449799713676E-5 3.999127923504148E-6 9 0.0796719595346908 1.4663469052848542E-5 2.6660852823360984E-6 5.1988663005553925E-5 3.999127923504148E-6 10-11 0.08143090929971204 1.732955433518464E-5 2.6660852823360984E-6 5.532126960847405E-5 3.999127923504148E-6 12-13 0.08391636730416988 1.732955433518464E-5 2.6660852823360984E-6 6.398604677606636E-5 4.665649244088172E-6 14-15 0.08665643645309079 1.866259697635269E-5 2.6660852823360984E-6 8.198212243183503E-5 5.332170564672197E-6 16-17 0.08968510933382459 1.999563961752074E-5 2.6660852823360984E-6 1.0064471940818772E-4 5.332170564672197E-6 18-19 0.09331965009496929 2.2661724899856838E-5 2.6660852823360984E-6 1.1330862449928419E-4 6.665213205840246E-6 20-21 0.09710149206796305 2.3994767541024886E-5 2.6660852823360984E-6 1.2064035902570847E-4 6.665213205840246E-6 22-23 0.10088133447699504 2.866041678511306E-5 2.6660852823360984E-6 1.2797209355213274E-4 6.665213205840246E-6 24-25 0.10440723226288454 3.0659980746865135E-5 2.6660852823360984E-6 1.3463730675797298E-4 6.665213205840246E-6 26-27 0.10810242646420237 3.265954470861721E-5 2.6660852823360984E-6 1.4330208392556527E-4 6.665213205840246E-6 28-29 0.1121022209090271 3.665867263212136E-5 2.6660852823360984E-6 1.493007758108215E-4 6.665213205840246E-6 30-31 0.11616933400723081 3.732519395270538E-5 2.6660852823360984E-6 1.5996511694016592E-4 6.665213205840246E-6 32-33 0.12064635771759372 3.7991715273289406E-5 2.6660852823360984E-6 1.646307661842541E-4 7.998255847008296E-6 34-35 0.12537066083789328 3.865823659387343E-5 2.6660852823360984E-6 1.7396206467243044E-4 7.998255847008296E-6 36-37 0.13026292733098002 3.932475791445745E-5 3.3326066029201235E-6 1.866259697635269E-4 7.998255847008296E-6 38-39 0.13538447715834767 4.132432187620953E-5 3.999127923504148E-6 1.9595726825170323E-4 7.998255847008296E-6 40-41 0.14101391623200032 4.3323885837961596E-5 3.999127923504148E-6 1.9995639617520738E-4 7.998255847008296E-6 42-43 0.14707859372799437 4.5323449799713676E-5 3.999127923504148E-6 2.0328900277812753E-4 7.998255847008296E-6 44-45 0.153203258142841 4.932257772321782E-5 3.999127923504148E-6 2.1262030126630385E-4 7.998255847008296E-6 46-47 0.1598444765811402 5.1988663005553925E-5 3.999127923504148E-6 2.2261812107506423E-4 9.331298488176346E-6 48-49 0.1667329744293761 5.598779092905807E-5 3.999127923504148E-6 2.326159408838246E-4 1.0664341129344394E-5 50-51 0.17470057029563751 5.865387621139417E-5 3.999127923504148E-6 2.4727940993667314E-4 1.0664341129344394E-5 52-53 0.18266749964057838 5.998691885256222E-5 4.665649244088172E-6 2.5394462314251337E-4 1.1997383770512443E-5 54-55 0.19067175417947194 6.331952545548234E-5 5.332170564672197E-6 2.6127635766893766E-4 1.1997383770512443E-5 56-57 0.19935386090139945 7.731647318774687E-5 5.332170564672197E-6 2.6660852823360984E-4 1.1997383770512443E-5 58-59 0.2089724300787475 8.531472903475516E-5 5.332170564672197E-6 2.679415708747779E-4 1.1997383770512443E-5 60-61 0.21898424683524015 8.798081431709125E-5 5.332170564672197E-6 2.732737414394501E-4 1.2663905091096469E-5 62-63 0.23043041747362958 9.997819808760369E-5 5.9986918852562215E-6 2.832715612482105E-4 1.4663469052848542E-5 64-65 0.2425291124848708 1.0397732601110784E-4 6.665213205840246E-6 2.952689450187229E-4 1.5996511694016593E-5 66-67 0.25608948875215276 1.0930949657578004E-4 6.665213205840246E-6 3.0460024350689925E-4 1.5996511694016593E-5 68-69 0.27154011948461104 1.2064035902570845E-4 6.665213205840246E-6 3.099324140715715E-4 1.5996511694016593E-5 70-71 0.29008207610193804 1.2797209355213274E-4 6.665213205840246E-6 3.1993023388033183E-4 1.5996511694016593E-5 72-73 0.3106929148983578 1.2930513619330077E-4 6.665213205840246E-6 3.292615323685082E-4 1.5996511694016593E-5 74-75 0.3346656872358035 1.2930513619330077E-4 6.665213205840246E-6 3.4525804406252477E-4 1.5996511694016593E-5 76-77 0.36392863929472447 1.2930513619330077E-4 6.665213205840246E-6 3.5792194915362124E-4 1.732955433518464E-5 78-79 0.39928892839434815 1.2930513619330077E-4 6.665213205840246E-6 3.685862902829656E-4 1.7996075655768666E-5 80-81 0.44218357450185364 1.2930513619330077E-4 6.665213205840246E-6 3.752515034888059E-4 1.866259697635269E-5 82-83 0.49594318465619935 1.2930513619330077E-4 6.665213205840246E-6 3.872488872593183E-4 1.866259697635269E-5 84-85 0.5666324363533792 1.2930513619330077E-4 6.665213205840246E-6 3.9124801518282247E-4 1.866259697635269E-5 86-87 0.6585670551441749 1.319712214756369E-4 6.665213205840246E-6 3.9991279235041476E-4 1.866259697635269E-5 88-89 0.7695008641782182 1.319712214756369E-4 6.665213205840246E-6 4.0724452687683905E-4 1.866259697635269E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGAGA 42125 0.0 35.42929 1 CGGACGT 6640 0.0 34.023186 1 CGCGTGC 8620 0.0 32.71876 1 CGGCGAG 14925 0.0 32.599483 1 CGGCAAG 18605 0.0 32.44004 1 GGAGAAT 141515 0.0 30.970076 1 GAATTGT 147000 0.0 30.525282 4 AATTGTA 156190 0.0 29.994312 5 GAGAATT 148850 0.0 29.03418 2 AGAATTG 152090 0.0 27.930555 3 ATTGTAA 177800 0.0 27.855486 6 CGGAGAA 54255 0.0 27.316893 2 TGCCGTC 39840 0.0 27.000257 48-49 CGCGTGT 278560 0.0 26.416615 1 TTGTAAT 188710 0.0 26.079487 7 GTAATAC 189025 0.0 26.03854 9 TATGCCG 41965 0.0 25.942766 46-47 CGTATGC 42175 0.0 25.829931 44-45 CGGAGAT 25480 0.0 25.684341 1 CCGTCTT 42280 0.0 25.555521 50-51 >>END_MODULE