FastQCFastQC Report
Fri 27 May 2016
SRR1233071_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233071_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37114114
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC503520.13566806417634003Illumina Single End PCR Primer 1 (100% over 50bp)
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC393620.10605668776034907No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN384020.10347007071218244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT268700.064.758411
GCGTGTT362350.046.0973432
GTTTTTT5837250.041.644581
CGCCGTA158700.031.10078646-47
GGCGCCG38250.030.79300744-45
GTCGCCG156050.029.23246644-45
CCGTATC169250.029.17646448-49
TCCGATC30600.028.9063281
CGTGTTT576800.028.5267283
CTGGAAT691900.028.4206241
GCGCCGT24450.028.06860544-45
CCGATCT33950.026.7421282
GAGCGGC32800.024.6223349
TGGTCGC170550.024.3196442-43
GATCGGG28250.024.0723781
CGCGTTT13550.023.865531
GGGCGCC59750.023.4439142-43
TCGGTGG214050.022.50645438-39
CGCCGGA19950.021.90673446-47
TATGGCG695200.021.6545437
Produced by FastQC (version 0.11.5)