##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233071_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 37114114 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.055611000170984 34.0 31.0 34.0 30.0 34.0 2 32.3017256184534 34.0 31.0 34.0 31.0 34.0 3 32.289553726110775 34.0 31.0 34.0 30.0 34.0 4 35.66255239179359 37.0 35.0 37.0 35.0 37.0 5 35.63903777953584 37.0 35.0 37.0 35.0 37.0 6 35.650562047635034 37.0 35.0 37.0 35.0 37.0 7 35.73436224289229 37.0 36.0 37.0 35.0 37.0 8 35.70416995539756 37.0 36.0 37.0 35.0 37.0 9 37.39357496180563 39.0 38.0 39.0 35.0 39.0 10-11 37.2709007548988 39.0 37.5 39.0 34.0 39.0 12-13 37.18940267575834 39.0 37.0 39.0 34.0 39.0 14-15 38.512560949184994 40.5 38.0 41.0 34.0 41.0 16-17 38.268090368532036 40.0 38.0 41.0 33.5 41.0 18-19 37.95153126920934 40.0 38.0 41.0 33.0 41.0 20-21 37.68580556712199 40.0 38.0 41.0 32.5 41.0 22-23 37.370661360796596 40.0 37.5 41.0 32.0 41.0 24-25 36.98673008872043 40.0 37.0 41.0 31.0 41.0 26-27 36.80500383223482 40.0 37.5 41.0 30.0 41.0 28-29 36.5422194235864 40.0 37.0 41.0 29.5 41.0 30-31 36.350609137537276 40.0 37.0 41.0 28.0 41.0 32-33 36.219821036277466 40.0 37.0 41.0 27.0 41.0 34-35 36.07705990233257 40.0 37.0 41.0 27.0 41.0 36-37 35.96519303949974 40.0 37.0 41.0 26.0 41.0 38-39 35.76959627811673 40.0 37.0 41.0 25.0 41.0 40-41 35.6709468532645 40.0 36.5 41.0 25.0 41.0 42-43 35.505115843530575 40.0 36.0 41.0 24.0 41.0 44-45 35.41544773236403 40.0 36.0 41.0 24.0 41.0 46-47 35.47261603766158 40.0 36.0 41.0 24.0 41.0 48-49 35.457687997078416 40.0 36.0 41.0 24.0 41.0 50-51 35.06125012171919 39.5 35.5 40.5 23.5 41.0 52-53 35.117390785618646 39.5 36.0 40.5 24.0 41.0 54-55 35.28117533669267 40.0 36.0 41.0 23.0 41.0 56-57 35.19938173655446 40.0 36.0 41.0 23.0 41.0 58-59 35.04255383814362 40.0 35.0 41.0 22.5 41.0 60-61 34.85136267836005 39.0 35.0 41.0 22.0 41.0 62-63 34.60852127576048 39.0 35.0 41.0 20.0 41.0 64-65 34.37314352970948 39.0 35.0 41.0 19.5 41.0 66-67 34.08240813723857 38.5 34.0 41.0 18.0 41.0 68-69 33.782482224956254 38.0 34.0 40.0 15.5 41.0 70-71 33.44553217678859 37.0 34.0 40.0 13.0 41.0 72-73 33.052218436360896 37.0 34.0 39.5 9.0 41.0 74-75 32.70464748262615 36.0 33.0 39.0 8.0 41.0 76-77 32.26701584469994 36.0 33.0 39.0 7.0 40.5 78-79 31.90209333839951 35.5 32.5 38.0 6.0 40.0 80-81 31.512493077431404 35.0 32.0 37.0 2.0 39.0 82-83 31.081055821513075 35.0 32.0 37.0 2.0 39.0 84-85 30.722850288167997 35.0 32.0 36.0 2.0 38.5 86-87 30.378722094780436 35.0 31.0 36.0 2.0 37.0 88-89 30.026713085485483 35.0 31.0 35.5 2.0 37.0 90-91 29.727281284419185 35.0 31.0 35.0 2.0 36.5 92-93 29.44101440761862 35.0 31.0 35.0 2.0 36.0 94-95 29.17042563645733 34.0 31.0 35.0 2.0 36.0 96-97 28.86376805330716 34.0 30.0 35.0 2.0 36.0 98-99 28.467122642345714 34.0 30.0 35.0 2.0 35.0 100-101 26.794557038327792 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28716452511653756 1101 2 0.22705280544835915 1101 3 0.25650666034002967 1101 4 0.18743121769069404 1101 5 0.17990759667091538 1101 6 0.21242541333394627 1101 7 0.19680174293176833 1101 8 0.24266191099688683 1101 9 0.3090286674503915 1101 10-11 0.3137233165716964 1101 12-13 0.32633505774196436 1101 14-15 0.4704109535751897 1101 16-17 0.49783654642131836 1101 18-19 0.47662481681829405 1101 20-21 0.5369150089295118 1101 22-23 0.47379249451032734 1101 24-25 0.4907231559340133 1101 26-27 0.39907310906306037 1101 28-29 0.45481097636921675 1101 30-31 0.43195438162789657 1101 32-33 0.4372756435231153 1101 34-35 0.4549204128104378 1101 36-37 0.462055675176714 1101 38-39 0.5787376981935424 1101 40-41 0.4543890706197047 1101 42-43 0.4829038754047801 1101 44-45 0.41030384487531535 1101 46-47 0.4284067593824048 1101 48-49 0.5126871697831135 1101 50-51 0.45340396965867313 1101 52-53 0.4226622379878009 1101 54-55 0.4826052729941068 1101 56-57 0.4900588232174172 1101 58-59 0.5259871591490466 1101 60-61 0.47055294185379637 1101 62-63 0.544223926639468 1101 64-65 0.4521824902485321 1101 66-67 0.4361118125765273 1101 68-69 0.5149855702064841 1101 70-71 0.5252768103669538 1101 72-73 0.527974326310293 1101 74-75 0.46479168465237564 1101 76-77 0.4490573800589601 1101 78-79 0.47404341957904705 1101 80-81 0.48991656872152234 1101 82-83 0.49076027709680403 1101 84-85 0.5183069978712354 1101 86-87 0.5715841339874039 1101 88-89 0.5019473388558424 1101 90-91 0.5118709503666672 1101 92-93 0.4912426920135857 1101 94-95 0.48237948986064794 1101 96-97 0.518448809324628 1101 98-99 0.5875362837315947 1101 100-101 0.8158079637487532 1102 1 0.17276599507467694 1102 2 0.15396219398370192 1102 3 0.2093504820707679 1102 4 0.17570589749736598 1102 5 0.16853003037441283 1102 6 0.17641077326460675 1102 7 0.16360670152428014 1102 8 0.1934012644706229 1102 9 0.26045661201732884 1102 10-11 0.2706689143736938 1102 12-13 0.25823740554363184 1102 14-15 0.36295036101278555 1102 16-17 0.40077898182854454 1102 18-19 0.41997042979475907 1102 20-21 0.44545323747978927 1102 22-23 0.4225959446234455 1102 24-25 0.4882960960828058 1102 26-27 0.4526475891864834 1102 28-29 0.46136889706824746 1102 30-31 0.438449943091193 1102 32-33 0.4504417280603761 1102 34-35 0.4595880502060794 1102 36-37 0.4542271634027344 1102 38-39 0.5264827655861879 1102 40-41 0.49194864404238103 1102 42-43 0.5560093914325179 1102 44-45 0.46376646047345105 1102 46-47 0.48239245871459957 1102 48-49 0.5412059123244859 1102 50-51 0.5128848213027837 1102 52-53 0.4873439963455226 1102 54-55 0.5138956302280846 1102 56-57 0.49232735758276647 1102 58-59 0.4538079684429661 1102 60-61 0.44710881921670165 1102 62-63 0.5323508596170328 1102 64-65 0.521675518562688 1102 66-67 0.551056621366321 1102 68-69 0.5737916050372078 1102 70-71 0.5433499773153656 1102 72-73 0.6061761592118913 1102 74-75 0.534931684218364 1102 76-77 0.526434747667686 1102 78-79 0.5214126925534046 1102 80-81 0.5366337478588044 1102 82-83 0.5362062525344413 1102 84-85 0.48603300557844165 1102 86-87 0.48890167669007667 1102 88-89 0.45208301074039525 1102 90-91 0.49553441057800285 1102 92-93 0.38700756476951526 1102 94-95 0.4316409341955847 1102 96-97 0.46770026871490344 1102 98-99 0.5090811123614891 1102 100-101 0.6961456584783576 1103 1 0.22940202351005468 1103 2 0.16712685086870493 1103 3 0.18715994327027374 1103 4 0.13258268924522554 1103 5 0.15233238573156882 1103 6 0.18046305497172455 1103 7 0.1659506593222133 1103 8 0.19172052350060653 1103 9 0.22388345119404107 1103 10-11 0.2807432574695028 1103 12-13 0.2356669376517786 1103 14-15 0.39455996101619206 1103 16-17 0.36348666434522414 1103 18-19 0.35022951903846433 1103 20-21 0.3665057614187148 1103 22-23 0.37669251391383796 1103 24-25 0.34330976005440306 1103 26-27 0.2730415152247332 1103 28-29 0.34596780661691184 1103 30-31 0.28271875229926025 1103 32-33 0.26302195175832566 1103 34-35 0.25902647987344096 1103 36-37 0.2493020678598299 1103 38-39 0.3264714404372526 1103 40-41 0.2392712626551372 1103 42-43 0.3095466695785163 1103 44-45 0.2210117128722331 1103 46-47 0.2661644554100988 1103 48-49 0.25937761491072564 1103 50-51 0.2266299567092247 1103 52-53 0.21301973577213573 1103 54-55 0.25218647759994894 1103 56-57 0.21800349139788722 1103 58-59 0.2804863428124236 1103 60-61 0.24758199944893278 1103 62-63 0.27348315444380233 1103 64-65 0.31350295619152746 1103 66-67 0.3062562969249072 1103 68-69 0.3211249934208311 1103 70-71 0.3123514178881095 1103 72-73 0.377882012056979 1103 74-75 0.29535151209456956 1103 76-77 0.3089802050934978 1103 78-79 0.3103625553027882 1103 80-81 0.29934715556699487 1103 82-83 0.26386527787678915 1103 84-85 0.3146147792825147 1103 86-87 0.36138307845110873 1103 88-89 0.3305250880827941 1103 90-91 0.3058098588413216 1103 92-93 0.2581530697965029 1103 94-95 0.21640679082443626 1103 96-97 0.23439036445450157 1103 98-99 0.2680760639765971 1103 100-101 0.5262681966761065 1104 1 0.32341956808963346 1104 2 0.2771219881835165 1104 3 0.30988563117545453 1104 4 0.23363849710248985 1104 5 0.2608282849204713 1104 6 0.29152908082672724 1104 7 0.22687724180356383 1104 8 0.2702109727559474 1104 9 0.3535789521305972 1104 10-11 0.3695547080245589 1104 12-13 0.37398607690168006 1104 14-15 0.5007819263780604 1104 16-17 0.5249423380787519 1104 18-19 0.5069268399052262 1104 20-21 0.5539488720842343 1104 22-23 0.536532161872195 1104 24-25 0.5354694340483519 1104 26-27 0.5219363235098697 1104 28-29 0.5512955697082518 1104 30-31 0.5891249915733496 1104 32-33 0.5985472163887806 1104 34-35 0.5845579098031877 1104 36-37 0.5638074166357967 1104 38-39 0.7229563541242712 1104 40-41 0.6665928364524447 1104 42-43 0.6466994119294398 1104 44-45 0.6236269888656452 1104 46-47 0.6395618100641443 1104 48-49 0.6091810385736949 1104 50-51 0.6009044835435375 1104 52-53 0.6256208447744527 1104 54-55 0.6492033364037937 1104 56-57 0.6476670444350319 1104 58-59 0.6158916784548651 1104 60-61 0.6220466626172296 1104 62-63 0.671901347491243 1104 64-65 0.6543294182777402 1104 66-67 0.6307829766370077 1104 68-69 0.6563619441844608 1104 70-71 0.6451102384019904 1104 72-73 0.7049637986888726 1104 74-75 0.626831231283397 1104 76-77 0.6852893061801382 1104 78-79 0.5818962109911752 1104 80-81 0.5910629060630832 1104 82-83 0.5709302955242634 1104 84-85 0.5202016400505691 1104 86-87 0.6018382624141445 1104 88-89 0.579380242432137 1104 90-91 0.49855492208759244 1104 92-93 0.5501392072289235 1104 94-95 0.5926802914824449 1104 96-97 0.6472833635880626 1104 98-99 0.727826994246854 1104 100-101 0.9808589154632941 1105 1 0.25650271063716445 1105 2 0.15592435923178272 1105 3 0.17863849858363778 1105 4 0.1927631277823565 1105 5 0.19458843474060217 1105 6 0.20499588603858854 1105 7 0.22124865431757712 1105 8 0.2213571439630968 1105 9 0.2506871636313406 1105 10-11 0.3014908975187396 1105 12-13 0.33566836669516675 1105 14-15 0.38413593519994294 1105 16-17 0.35722166607529715 1105 18-19 0.43235959689015857 1105 20-21 0.4924731634326278 1105 22-23 0.4380914544822829 1105 24-25 0.48889291373817656 1105 26-27 0.5229705016356974 1105 28-29 0.5040181217196746 1105 30-31 0.5465954196458966 1105 32-33 0.49399823076013405 1105 34-35 0.5326221250293983 1105 36-37 0.5735875154918162 1105 38-39 0.6928073015328025 1105 40-41 0.6200510853029542 1105 42-43 0.7083333907815685 1105 44-45 0.578008889361044 1105 46-47 0.5684861440103859 1105 48-49 0.5648413547073261 1105 50-51 0.5183859106080675 1105 52-53 0.4849347333371554 1105 54-55 0.5140191278166313 1105 56-57 0.5771214394480566 1105 58-59 0.6030116207147529 1105 60-61 0.6074165928939195 1105 62-63 0.6752381904453202 1105 64-65 0.6270014507600337 1105 66-67 0.6202824499384008 1105 68-69 0.662131683016149 1105 70-71 0.6507041180485729 1105 72-73 0.6721090727658279 1105 74-75 0.6162328971574809 1105 76-77 0.679584419702671 1105 78-79 0.607995233806065 1105 80-81 0.6022863594460937 1105 82-83 0.6841126313598167 1105 84-85 0.6675380228907102 1105 86-87 0.6604495625140032 1105 88-89 0.6508216643170712 1105 90-91 0.7213385848336848 1105 92-93 0.7092152714621385 1105 94-95 0.6525009492491485 1105 96-97 0.715807993225468 1105 98-99 0.7022399222833791 1105 100-101 0.8677315234865866 1106 1 0.16179654259322263 1106 2 0.09326360029368885 1106 3 0.1252543445908998 1106 4 0.1083657376820355 1106 5 0.07514622512522351 1106 6 0.12821680999786622 1106 7 0.1403605346430723 1106 8 0.14694562603526862 1106 9 0.2045898126078498 1106 10-11 0.21916552317643578 1106 12-13 0.2352289288095406 1106 14-15 0.25308448740666023 1106 16-17 0.29188096251266415 1106 18-19 0.29967788952992436 1106 20-21 0.3446786044633896 1106 22-23 0.31812121188861653 1106 24-25 0.3196095788715283 1106 26-27 0.3098310513300504 1106 28-29 0.3708813647661131 1106 30-31 0.3826201889318881 1106 32-33 0.339843953404646 1106 34-35 0.33924962087154853 1106 36-37 0.37032925948523143 1106 38-39 0.46666496311269867 1106 40-41 0.43748143033342757 1106 42-43 0.4457277795122465 1106 44-45 0.38388473004634704 1106 46-47 0.41429091788636896 1106 48-49 0.4650815986829926 1106 50-51 0.44995350378461296 1106 52-53 0.4198449816036174 1106 54-55 0.46998754136200205 1106 56-57 0.46164448911553535 1106 58-59 0.4244513407748016 1106 60-61 0.4118469898989119 1106 62-63 0.4919742956445745 1106 64-65 0.4740924151443764 1106 66-67 0.46585245276903464 1106 68-69 0.4810048787661074 1106 70-71 0.46355130745158135 1106 72-73 0.45503377106719256 1106 74-75 0.5002027369671893 1106 76-77 0.5412480750566502 1106 78-79 0.531625714629115 1106 80-81 0.48736793789700883 1106 82-83 0.4762813642680257 1106 84-85 0.45673810926179215 1106 86-87 0.47552342211958276 1106 88-89 0.4783871692727786 1106 90-91 0.4984433070225762 1106 92-93 0.4409050273941517 1106 94-95 0.37642515360733597 1106 96-97 0.4378824084913937 1106 98-99 0.5010158743025102 1106 100-101 0.7064428035958059 1107 1 0.010271827207382955 1107 2 -0.011447846888131608 1107 3 0.03623456191849783 1107 4 0.036933507562089574 1107 5 0.05041054236168918 1107 6 0.07544899218368073 1107 7 0.0724650877595181 1107 8 0.08914459609209047 1107 9 0.1375766183718028 1107 10-11 0.13402264636378192 1107 12-13 0.14658289646074962 1107 14-15 0.17914898200418605 1107 16-17 0.14474547153510997 1107 18-19 0.1490830824904421 1107 20-21 0.12578766051990442 1107 22-23 0.15712051523579618 1107 24-25 0.16725106550959623 1107 26-27 0.09169746177756366 1107 28-29 0.12303586817033363 1107 30-31 0.168105540362248 1107 32-33 0.13271376510251542 1107 34-35 0.16864995318443476 1107 36-37 0.16181392861713562 1107 38-39 0.3314982983238721 1107 40-41 0.23679780121175753 1107 42-43 0.26335529998917906 1107 44-45 0.18865150788768403 1107 46-47 0.1338689812556737 1107 48-49 0.12817777501825844 1107 50-51 0.15484764071796775 1107 52-53 0.13198049412327606 1107 54-55 0.21088679680910616 1107 56-57 0.21414554611856573 1107 58-59 0.2140889466252105 1107 60-61 0.22615880209603034 1107 62-63 0.27074759232421997 1107 64-65 0.2320411852161044 1107 66-67 0.23715819754130507 1107 68-69 0.21559381191602967 1107 70-71 0.2527206784419107 1107 72-73 0.25313243161922827 1107 74-75 0.2758510996657151 1107 76-77 0.3193907539924439 1107 78-79 0.24371675377275892 1107 80-81 0.2537181977577099 1107 82-83 0.3248378207266285 1107 84-85 0.284563780453702 1107 86-87 0.2954559294694441 1107 88-89 0.240991765070941 1107 90-91 0.30942698691639237 1107 92-93 0.27192166312766375 1107 94-95 0.24161677902287337 1107 96-97 0.3069952887743348 1107 98-99 0.2982706735928069 1107 100-101 0.5760452852251134 1108 1 -0.001405057303237811 1108 2 5.496745380995094E-4 1108 3 0.005852509034042441 1108 4 0.0037500039652655914 1108 5 0.029601132335344005 1108 6 0.05559820399140847 1108 7 0.04497244656935351 1108 8 0.08403358379280235 1108 9 0.05963511953262213 1108 10-11 0.07510029757771264 1108 12-13 0.03954993893810155 1108 14-15 0.023437228864317206 1108 16-17 0.0653693658195138 1108 18-19 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-1.4768228864552668 2316 68-69 -1.3561043097238823 2316 70-71 -1.2833968752295632 2316 72-73 -1.1919796934565703 2316 74-75 -1.206161324696673 2316 76-77 -1.1425957927759178 2316 78-79 -1.127855883346708 2316 80-81 -1.183202675603038 2316 82-83 -1.0812027691831751 2316 84-85 -1.263811224650489 2316 86-87 -1.335562567820677 2316 88-89 -1.4954928872569226 2316 90-91 -1.4094637186680359 2316 92-93 -1.5804220252903605 2316 94-95 -1.6437172979599666 2316 96-97 -1.602533819257463 2316 98-99 -1.5702596426452509 2316 100-101 -1.8965492488633018 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 192445.0 3 30326.0 4 26084.0 5 36365.0 6 79999.0 7 157872.0 8 261822.0 9 402363.0 10 460634.0 11 355795.0 12 244647.0 13 179824.0 14 147567.0 15 128904.0 16 122790.0 17 119949.0 18 121746.0 19 126356.0 20 137926.0 21 152247.0 22 172428.0 23 197298.0 24 230173.0 25 256554.0 26 280800.0 27 328483.0 28 387177.0 29 463992.0 30 564205.0 31 693671.0 32 872142.0 33 1120059.0 34 1498486.0 35 2117391.0 36 3316850.0 37 6240946.0 38 1.047765E7 39 4397856.0 40 12292.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.73259461075801 16.53888731478236 8.51092959640744 42.21758847805219 2 13.350549682587861 16.22631908537825 59.01603683095898 11.407094401074904 3 18.34447781002657 20.406726566202458 41.346707425874456 19.902088197896507 4 18.842152316706972 28.464620167961147 37.39960677066897 15.293620744662913 5 19.817602016313597 30.324000492492875 36.158233518733255 13.700163972460278 6 15.144603079214802 32.90537119369796 37.553741206671674 14.396284520415556 7 12.84042158486465 16.863985997112565 54.25389474615459 16.041697671868196 8 13.28481574435711 20.957288479272577 46.717446146224255 19.040449630146053 9 16.1542203708687 19.487815195660197 45.28526715268072 19.072697280790383 10-11 18.842422540396914 30.68904263787251 34.35394850902854 16.114586312702038 12-13 16.874227667892153 25.14675331239352 41.03143849526638 16.947580524447943 14-15 17.28420026601813 25.7633519396955 40.1379974786258 16.81445031566057 16-17 18.302438711888932 26.568704645949758 37.50702003171485 17.621836610446458 18-19 19.21741224983121 26.84521261183086 36.195663844705386 17.74171129363255 20-21 18.80517380194469 27.540904223723455 35.42421877767007 18.229703196661788 22-23 19.020640734964125 28.15158267882017 35.61880518219344 17.208971404022265 24-25 18.823871356363682 28.43773197428216 34.912430484657655 17.825966184696508 26-27 19.067999395551453 28.81760549113732 33.99264353672525 18.12175157658598 28-29 19.5946473538159 29.092604048079767 32.58806994686182 18.724678651242506 30-31 19.158200096327324 28.710828487608904 33.161053957312596 18.969917458751176 32-33 19.50885743275387 29.11576329276399 32.91036972478402 18.46500954969812 34-35 18.73624486853197 30.050748062751136 32.10485534515936 19.108151723557533 36-37 18.806140348556326 29.359818533389138 32.47627118177326 19.357769936281287 38-39 19.359141548190262 28.76027995240368 32.67583491409785 19.204743585308215 40-41 20.09838274703434 28.881482291453736 32.05768326679209 18.962451694719835 42-43 19.976273725801853 29.56698119059018 31.82775605922305 18.62898902438491 44-45 19.70771601443535 29.515579861411496 31.80825201990329 18.968452104249867 46-47 19.264503646914246 29.27083982808773 31.877593346996274 19.587063178001753 48-49 19.93969745301191 29.489728085776008 30.763148564093655 19.80742589711843 50-51 19.549127139952088 29.705478941772213 31.356570697015485 19.388823221260214 52-53 19.3843249080377 30.008781705578485 31.67336980931771 18.933523577066104 54-55 19.34834980609546 30.05616012668815 31.32987992378099 19.2656101434354 56-57 19.92405588682539 29.51639606749228 31.15422298839765 19.405325057284678 58-59 19.669717139069142 30.02169468242793 30.346514111453526 19.962074067049404 60-61 19.923694147814665 30.405033863162036 30.378823588367116 19.292448400656188 62-63 18.93226902497448 31.461366032060784 30.487069184758965 19.119295758205773 64-65 18.83716603882651 31.18637762964365 30.656578947581554 19.31987738394829 66-67 19.154421979647356 30.49881806273519 30.82375108795504 19.523008869662416 68-69 19.90555444129358 30.7507866571033 29.944521002896252 19.399137898706865 70-71 19.41335936244868 31.281593716290747 29.829706402919363 19.475340518341213 72-73 19.16445570147312 31.401481253722395 30.18441031113264 19.249652733671837 74-75 19.435929632360004 30.661718714631665 30.35328386173639 19.54906779127194 76-77 19.818380978380116 30.883204527044057 29.71122872359524 19.587185770980593 78-79 19.639636898175 31.263325641300465 29.514082654708663 19.582954805815874 80-81 19.689724884379398 31.09970909455239 30.32094957270381 18.889616448364404 82-83 19.5414811089934 31.43969574930351 29.88906653858899 19.1297566031141 84-85 19.495010148049687 31.52262555077946 29.642846601724926 19.339517699445928 86-87 20.025267434696648 31.652120989255717 28.915785211119736 19.406826364927895 88-89 19.69958329904922 31.659018401922413 29.33523761600756 19.30616068302081 90-91 19.810054234959104 31.718411713404944 29.186761429213252 19.2847726224227 92-93 19.615585038932466 32.09620777690182 28.981028799462866 19.30717838470285 94-95 19.492378603299628 32.252851523145274 28.730640175937623 19.52412969761747 96-97 19.85081006809341 31.653169300815087 29.039435344650265 19.45658528644124 98-99 20.306271043473327 32.025461762375855 28.4814618181021 19.18680537604872 100-101 20.716428754053904 32.468612270162865 27.763893541449896 19.051065434333335 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 45085.0 1 29147.5 2 11802.5 3 11448.5 4 13890.5 5 17172.5 6 21116.0 7 25407.0 8 30292.5 9 35597.0 10 40947.0 11 47070.5 12 53958.5 13 61099.0 14 69016.5 15 78221.0 16 88865.0 17 103174.5 18 121854.5 19 143525.5 20 171114.0 21 205756.0 22 246891.5 23 301429.5 24 372298.0 25 453242.0 26 540284.5 27 642551.5 28 756171.5 29 874907.0 30 1014859.0 31 1157134.0 32 1307886.0 33 1481618.5 34 1630785.5 35 1746726.0 36 1834349.0 37 1820623.0 38 1697806.5 39 1540137.5 40 1417530.5 41 1331863.5 42 1264011.0 43 1217597.0 44 1167509.5 45 1115376.5 46 1057762.5 47 993272.5 48 934256.0 49 874441.5 50 793099.0 51 699713.5 52 613892.0 53 542043.0 54 475650.0 55 407823.0 56 342417.5 57 279285.0 58 219881.0 59 166026.0 60 119436.0 61 83406.5 62 57949.0 63 38313.0 64 24642.0 65 16024.0 66 10508.0 67 7050.5 68 4832.0 69 3249.0 70 2413.0 71 1854.5 72 1449.0 73 1435.5 74 1296.0 75 877.0 76 686.0 77 579.0 78 466.0 79 357.5 80 260.0 81 181.0 82 152.5 83 128.5 84 92.5 85 70.0 86 56.0 87 42.0 88 32.5 89 23.5 90 17.0 91 15.0 92 13.5 93 10.0 94 9.0 95 5.0 96 4.0 97 5.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36024031181237415 2 0.3040649171902635 3 0.27820682988687273 4 0.2792738094192414 5 0.2759354567914514 6 0.21344440554340055 7 0.22642868424664536 8 0.22402528590605722 9 0.2234971849253898 10-11 0.22473525839792374 12-13 0.23237655626104936 14-15 0.2271561702914422 16-17 0.23691256646999576 18-19 0.2381506399425297 20-21 0.22793080820951295 22-23 0.20449902158515762 24-25 0.19768759669165212 26-27 0.17639785231030974 28-29 0.17271865899856859 30-31 0.19309365703839784 32-33 0.20556600111752635 34-35 0.20570476234458945 36-37 0.1997407239736344 38-39 0.1944664501488571 40-41 0.17961630446034627 42-43 0.17484453488503054 44-45 0.16446708117564116 46-47 0.17509241901881314 48-49 0.19640102414946506 50-51 0.200652775922389 52-53 0.22557995052771568 54-55 0.23476782983422426 56-57 0.23729786463446223 58-59 0.2172084722270347 60-61 0.20777001439398501 62-63 0.19998321932190002 64-65 0.19482615158211777 66-67 0.19902266830349233 68-69 0.19979191743604605 70-71 0.21195171195518772 72-73 0.24232560152183613 74-75 0.25059469289769387 76-77 0.23023181962527786 78-79 0.2117994787643321 80-81 0.20143145542959748 82-83 0.18840810803135433 84-85 0.17613110742721758 86-87 0.1971096494449524 88-89 0.17417228389178308 90-91 0.1677919618396387 92-93 0.2130806625210021 94-95 0.21331507469099223 96-97 0.21531566131418362 98-99 0.20452596551274269 100-101 0.1861946643802409 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.7114114E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.32797995515798 #Duplication Level Percentage of deduplicated Percentage of total 1 74.37920057393552 32.97079712122088 2 11.655044292193557 10.332891395216686 3 4.559817676941847 6.063825197479597 4 2.4178515379323287 4.287138980320486 5 1.492176867816553 3.3072593143061284 6 1.0287222711979291 2.736070813025183 7 0.750828945524046 2.3297911312859614 8 0.5393921578864407 1.9128131810207636 9 0.4392347806630276 1.752335149755501 >10 2.504131294108151 20.219394719813554 >50 0.16039987498887484 4.832035589477097 >100 0.06740317066041351 5.324974711407016 >500 0.003728448046653282 1.132768315083898 >1k 0.0018611483829118149 1.5228412188405032 >5k 1.278280650771741E-4 0.40730064517218245 >10k+ 7.913165667997977E-5 0.8677625165745646 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 50352 0.13566806417634003 Illumina Single End PCR Primer 1 (100% over 50bp) CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 39362 0.10605668776034907 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 38402 0.10347007071218244 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.1620057533907451 0.0 0.0 8.083178275520736E-6 0.0 2 0.163334089020689 0.0 0.0 1.3471963792534559E-5 0.0 3 0.1634876694079239 0.0 0.0 1.3471963792534559E-5 0.0 4 0.16556774061749124 0.0 0.0 1.3471963792534559E-5 0.0 5 0.16596381635299176 0.0 0.0 1.6166356551041473E-5 0.0 6 0.1662898378767711 0.0 0.0 1.6166356551041473E-5 0.0 7 0.1666077762222749 0.0 0.0 1.6166356551041473E-5 0.0 8 0.17001348866902763 0.0 0.0 1.8860749309548385E-5 0.0 9 0.17035567654935801 0.0 0.0 2.1555142068055297E-5 0.0 10-11 0.17155333413051432 1.347196379253456E-6 0.0 2.5596731205815664E-5 0.0 12-13 0.17328582867423428 2.694392758506912E-6 0.0 2.963832034357603E-5 1.347196379253456E-6 14-15 0.1754588564339701 2.694392758506912E-6 0.0 7.140140810043317E-5 2.694392758506912E-6 16-17 0.1781155276938579 2.694392758506912E-6 0.0 1.0912290671952993E-4 5.388785517013824E-6 18-19 0.18162497426181318 2.694392758506912E-6 0.0 1.1451169223654375E-4 5.388785517013824E-6 20-21 0.18571506246922664 2.694392758506912E-6 0.0 1.3471963792534557E-4 5.388785517013824E-6 22-23 0.19058113579109015 2.694392758506912E-6 0.0 1.6166356551041471E-4 5.388785517013824E-6 24-25 0.19482210999298002 2.694392758506912E-6 0.0 1.926490822332442E-4 5.388785517013824E-6 26-27 0.19858078789109718 2.694392758506912E-6 0.0 2.3306497361084787E-4 5.388785517013824E-6 28-29 0.20318146352624772 2.694392758506912E-6 0.0 3.408406839511244E-4 8.083178275520736E-6 30-31 0.20831562892758265 5.388785517013824E-6 0.0 3.9338134274200914E-4 8.083178275520736E-6 32-33 0.21417728037371442 5.388785517013824E-6 0.0 4.0820050291379714E-4 8.083178275520736E-6 34-35 0.22012515238811842 6.73598189626728E-6 0.0 4.122420920515575E-4 8.083178275520736E-6 36-37 0.2265202936004346 8.083178275520736E-6 0.0 4.2032527032707825E-4 8.083178275520736E-6 38-39 0.23375473815702566 8.083178275520736E-6 0.0 4.28408448602599E-4 8.083178275520736E-6 40-41 0.24233637909287017 8.083178275520736E-6 0.0 4.3379723411961284E-4 8.083178275520736E-6 42-43 0.25227329958624367 8.083178275520736E-6 0.0 4.499635906706543E-4 8.083178275520736E-6 44-45 0.2623611060740936 8.083178275520736E-6 0.0 4.499635906706543E-4 8.083178275520736E-6 46-47 0.2736142374299977 8.083178275520736E-6 0.0 4.566995725669216E-4 8.083178275520736E-6 48-49 0.2859639866386141 9.430374654774192E-6 0.0 4.6612994722169574E-4 8.083178275520736E-6 50-51 0.2997323336345844 1.0777571034027649E-5 0.0 4.742131254972165E-4 8.083178275520736E-6 52-53 0.3150203181463527 1.0777571034027649E-5 0.0 4.7690751825572343E-4 8.083178275520736E-6 54-55 0.3316123887532382 1.0777571034027649E-5 0.0 4.7690751825572343E-4 8.083178275520736E-6 56-57 0.3501902268231434 1.3471963792534559E-5 0.0 4.7825471463497685E-4 8.083178275520736E-6 58-59 0.37075652674882664 1.4819160171788015E-5 0.0 4.8768508928975107E-4 8.083178275520736E-6 60-61 0.39418831337318194 1.6166356551041473E-5 0.0 4.944210711860183E-4 8.083178275520736E-6 62-63 0.4211403241365266 2.1555142068055297E-5 0.0 5.038514458407925E-4 8.083178275520736E-6 64-65 0.45158830950403395 2.1555142068055297E-5 0.0 5.105874277370598E-4 8.083178275520736E-6 66-67 0.4856952802375937 2.1555142068055297E-5 0.0 5.159762132540737E-4 8.083178275520736E-6 68-69 0.5248596261788709 2.1555142068055297E-5 0.0 5.173234096333271E-4 8.083178275520736E-6 70-71 0.5713850531363891 2.290233844730875E-5 0.0 5.173234096333271E-4 8.083178275520736E-6 72-73 0.6264543995311326 2.5596731205815664E-5 0.0 5.213649987710875E-4 8.083178275520736E-6 74-75 0.6920076281492265 2.6943927585069118E-5 0.0 5.281009806673547E-4 8.083178275520736E-6 76-77 0.7673873071575951 2.8291123964322576E-5 0.0 5.37531355322129E-4 9.430374654774192E-6 78-79 0.8566916079419274 3.0985516722829484E-5 0.0 5.429201408391428E-4 1.0777571034027649E-5 80-81 0.9626997966326234 3.36799094813364E-5 0.0 5.523505154939169E-4 1.0777571034027649E-5 82-83 1.0901769068231024 3.772149861909677E-5 0.0 5.604336937694377E-4 1.0777571034027649E-5 84-85 1.2468518041411416 3.772149861909677E-5 0.0 5.739056575619723E-4 1.0777571034027649E-5 86-87 1.4358917472743657 3.772149861909677E-5 0.0 5.766000503204791E-4 1.0777571034027649E-5 88-89 1.6583556325768682 3.772149861909677E-5 0.0 5.873776213545067E-4 1.0777571034027649E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 26870 0.0 64.75841 1 GCGTGTT 36235 0.0 46.097343 2 GTTTTTT 583725 0.0 41.64458 1 CGCCGTA 15870 0.0 31.100786 46-47 GGCGCCG 3825 0.0 30.793007 44-45 GTCGCCG 15605 0.0 29.232466 44-45 CCGTATC 16925 0.0 29.176464 48-49 TCCGATC 3060 0.0 28.906328 1 CGTGTTT 57680 0.0 28.526728 3 CTGGAAT 69190 0.0 28.420624 1 GCGCCGT 2445 0.0 28.068605 44-45 CCGATCT 3395 0.0 26.742128 2 GAGCGGC 3280 0.0 24.622334 9 TGGTCGC 17055 0.0 24.31964 42-43 GATCGGG 2825 0.0 24.072378 1 CGCGTTT 1355 0.0 23.86553 1 GGGCGCC 5975 0.0 23.44391 42-43 TCGGTGG 21405 0.0 22.506454 38-39 CGCCGGA 1995 0.0 21.906734 46-47 TATGGCG 69520 0.0 21.654543 7 >>END_MODULE