FastQCFastQC Report
Fri 27 May 2016
SRR1233071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233071_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37114114
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC935420.2520388874162535TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG564560.15211463757426621TruSeq Adapter, Index 2 (100% over 49bp)
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC405010.10912560111228845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT245500.067.7288361
GCGTGTT340900.046.7734872
GTTTTTT4781600.037.8857961
TATGCCG254300.031.88230946-47
TGCCGTC256250.031.44212548-49
CCGTCTT266900.030.22313150-51
CTCGTAT270400.029.82612442-43
CGTGTTT541050.028.8096683
CGTATGC284250.028.64020744-45
CTGGAAT698250.028.1624721
CGATGTA306700.026.23154434-35
ACCGATG314850.025.84671232-33
ATCTCGT312450.025.67486640-41
CGCGTTT12950.025.3633041
TCACCGA336750.024.17991430-31
TATGGCG726050.021.2390047
GTATCTC387900.020.75877438-39
ATGGCGA747200.020.6123968
TGGCGAG734000.020.6077259
GTGTTTT1410300.020.2282941
Produced by FastQC (version 0.11.5)