FastQCFastQC Report
Fri 27 May 2016
SRR1233070_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233070_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60674279
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC899270.14821272124222523No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG703530.11595193409714849No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA621990.10251296105224424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT586000.043.9187741
GCGTGTT644850.036.4115222
GTTTTTT5257050.033.9145661
CTGGAAT1415300.027.704031
CGTGTTT804650.027.677633
CGCGTGC43400.026.4824711
CGCGTTT44400.020.4363291
CGGCGAG70250.019.5702151
CCCGCGT40700.019.0513881
CCGGCAT71550.018.292341
CTCGCGT207400.018.003751
CGCGTAT37250.017.7156431
CGCGGTT22250.017.5481071
CCGAAAC53650.017.2203311
TATGGCG2096200.017.082677
CGGCAAG69100.016.9513191
ATGGCGA2123700.016.7831138
CCGATCT37050.016.6148382
TGGCGAG2127250.016.557089
GAATATG2637200.016.0130164
Produced by FastQC (version 0.11.5)