##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233070_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 60674279 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.93346866140758 31.0 30.0 34.0 16.0 34.0 2 26.038538603812665 31.0 27.0 34.0 2.0 34.0 3 26.96823209057004 31.0 27.0 34.0 2.0 34.0 4 30.691674918790547 35.0 32.0 37.0 10.0 37.0 5 30.42927908545893 35.0 32.0 37.0 2.0 37.0 6 31.05032107592082 35.0 32.0 37.0 10.0 37.0 7 30.81744026987119 35.0 32.0 37.0 8.0 37.0 8 30.72447178152706 36.0 32.0 37.0 2.0 37.0 9 32.59953346952833 38.0 32.0 39.0 17.0 39.0 10-11 32.75786043539141 38.0 33.0 39.0 13.5 39.0 12-13 32.20894529294695 38.0 32.0 39.0 6.5 39.0 14-15 32.97727903779458 38.0 32.0 41.0 6.0 41.0 16-17 32.956592850489415 38.0 32.0 40.0 10.0 41.0 18-19 33.54090962498293 38.5 32.5 40.5 14.5 41.0 20-21 33.9029799431156 39.0 33.5 41.0 17.0 41.0 22-23 33.848705445679876 39.0 33.0 41.0 17.0 41.0 24-25 33.880776481909244 39.0 33.0 41.0 15.5 41.0 26-27 33.993310015270225 39.0 33.5 41.0 16.0 41.0 28-29 34.06241270703852 39.0 34.0 41.0 16.0 41.0 30-31 34.213340483205414 39.0 34.0 41.0 16.0 41.0 32-33 33.948287304740774 38.5 34.0 40.0 15.5 41.0 34-35 33.93580689273621 38.0 33.5 40.0 15.5 41.0 36-37 33.73128371249372 38.0 33.0 40.0 15.0 41.0 38-39 33.73041127526213 38.0 33.0 40.0 15.0 41.0 40-41 33.457401265534614 38.0 33.0 40.0 10.5 41.0 42-43 33.627035551588506 38.0 33.0 40.0 12.0 41.0 44-45 33.51625748202134 38.0 33.0 40.0 11.0 41.0 46-47 33.44455218001025 38.0 33.0 40.0 12.0 41.0 48-49 33.29054049410294 38.0 33.0 40.0 9.0 41.0 50-51 32.32546049702544 37.0 31.5 39.0 9.0 40.5 52-53 32.440533615570445 37.5 31.5 39.5 9.0 40.0 54-55 32.797139394108 38.0 32.0 40.0 8.0 41.0 56-57 32.828447982051834 38.0 32.0 40.0 8.0 41.0 58-59 32.681720676070995 38.0 31.5 40.0 7.5 41.0 60-61 32.382679932628456 37.0 31.0 40.0 7.0 41.0 62-63 32.08797484021194 37.0 31.0 40.0 4.0 41.0 64-65 31.806084370611146 36.5 31.0 39.5 2.0 41.0 66-67 31.441320382233137 36.0 30.0 39.0 2.0 41.0 68-69 30.934837577550777 35.5 29.0 39.0 2.0 40.5 70-71 30.58409034411435 35.0 29.0 39.0 2.0 40.0 72-73 30.307367789240644 35.0 29.0 38.0 2.0 40.0 74-75 29.979156101714864 35.0 29.0 37.5 2.0 40.0 76-77 29.46882394762367 34.5 28.5 37.0 2.0 39.0 78-79 28.993704614108395 34.0 28.0 36.0 2.0 39.0 80-81 28.553223335377417 34.0 27.0 36.0 2.0 38.5 82-83 28.228447477719513 34.0 26.5 35.5 2.0 37.0 84-85 27.886322555229704 34.0 26.0 35.0 2.0 37.0 86-87 27.351482371961932 34.0 26.0 35.0 2.0 36.5 88-89 26.83748163698822 33.0 25.0 35.0 2.0 36.0 90-91 26.437548734612896 33.0 24.5 35.0 2.0 36.0 92-93 26.085610049029178 33.0 23.5 35.0 2.0 35.5 94-95 25.575005547243503 33.0 20.0 35.0 2.0 35.0 96-97 24.961379788295467 32.0 14.5 35.0 2.0 35.0 98-99 24.048875257339276 32.0 2.0 35.0 2.0 35.0 100-101 20.6152726676818 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.7913567149375282 1101 2 7.334829828749157 1101 3 6.348178841035477 1101 4 6.221985574141332 1101 5 6.3450753921857626 1101 6 5.7170319252983575 1101 7 5.994759239353069 1101 8 6.038011324670187 1101 9 5.824860001964009 1101 10-11 5.645774984733759 1101 12-13 6.06235599101781 1101 14-15 6.56362876542353 1101 16-17 6.143033544439692 1101 18-19 5.435459060282021 1101 20-21 5.187484734104473 1101 22-23 5.0464851827576 1101 24-25 4.80017244919825 1101 26-27 4.5289850690432445 1101 28-29 4.153752314980714 1101 30-31 3.7827548373696445 1101 32-33 3.9641046407028213 1101 34-35 3.6860548023536595 1101 36-37 3.7534708032858504 1101 38-39 3.532882305577644 1101 40-41 3.8917379445063958 1101 42-43 3.763105173404334 1101 44-45 3.6560197380745265 1101 46-47 3.518468892129384 1101 48-49 3.605807061173458 1101 50-51 3.402210932518937 1101 52-53 3.3744677512603367 1101 54-55 3.5662349684092476 1101 56-57 3.4692616329688235 1101 58-59 3.353720608355818 1101 60-61 3.346096733414747 1101 62-63 3.2858303091798895 1101 64-65 3.31475799660754 1101 66-67 3.251879856361281 1101 68-69 3.400187615679478 1101 70-71 3.3640826599326523 1101 72-73 3.186353752009797 1101 74-75 2.9644080782268176 1101 76-77 3.0923497785314673 1101 78-79 3.074390886246036 1101 80-81 2.8576703544505726 1101 82-83 2.792160274631293 1101 84-85 2.781027876307544 1101 86-87 2.7360829455246822 1101 88-89 2.6773197546756506 1101 90-91 2.7375654611182085 1101 92-93 2.619959269899585 1101 94-95 2.580753661932711 1101 96-97 2.6374730467873313 1101 98-99 2.7508596521912914 1101 100-101 2.129914963809508 1102 1 3.638402570076696 1102 2 7.166192018546983 1102 3 6.195479211158073 1102 4 6.073592129559618 1102 5 6.2278921930211055 1102 6 5.600298964345967 1102 7 5.916778957392612 1102 8 5.998249810613395 1102 9 5.738800818394733 1102 10-11 5.505033032209358 1102 12-13 5.8765741199463335 1102 14-15 6.395866306025319 1102 16-17 5.968972341999429 1102 18-19 5.137917920597438 1102 20-21 4.9669952166248095 1102 22-23 4.832372611889738 1102 24-25 4.521632369259237 1102 26-27 4.238564729912831 1102 28-29 3.9293822766938007 1102 30-31 3.5664717837856372 1102 32-33 3.783491088846496 1102 34-35 3.503394041043876 1102 36-37 3.3798699305750475 1102 38-39 3.258054067455305 1102 40-41 3.631553667905017 1102 42-43 3.386366265031292 1102 44-45 3.473149853320294 1102 46-47 3.31171191827854 1102 48-49 3.3317044727931204 1102 50-51 3.1410131281297815 1102 52-53 3.156420694223474 1102 54-55 3.293460867908969 1102 56-57 3.1965264874327914 1102 58-59 3.1211417750320045 1102 60-61 3.105574764216165 1102 62-63 2.9499350632553067 1102 64-65 3.015530049021507 1102 66-67 2.9832445401198378 1102 68-69 3.256370251032166 1102 70-71 3.216736777146213 1102 72-73 3.0352393952217263 1102 74-75 2.7404484232302693 1102 76-77 2.804823767444951 1102 78-79 2.87654469171364 1102 80-81 2.719191467116495 1102 82-83 2.674910600676043 1102 84-85 2.63052141150553 1102 86-87 2.5564916754953977 1102 88-89 2.5250728351687854 1102 90-91 2.599430903711866 1102 92-93 2.4437297682782138 1102 94-95 2.423365350390437 1102 96-97 2.375713540336914 1102 98-99 2.514276669988238 1102 100-101 1.9956044904690486 1103 1 3.449190337127721 1103 2 6.972266235315999 1103 3 6.025664480713861 1103 4 5.964948716103816 1103 5 6.174924749055251 1103 6 5.540934553911441 1103 7 5.82848249798386 1103 8 5.933491792943755 1103 9 5.653870877467259 1103 10-11 5.364872868132739 1103 12-13 5.82775492464917 1103 14-15 6.22703851760609 1103 16-17 5.805838892294968 1103 18-19 5.1274643586713395 1103 20-21 4.884022072248385 1103 22-23 4.811240337514853 1103 24-25 4.429148213778305 1103 26-27 4.241622864160675 1103 28-29 3.94378942680175 1103 30-31 3.52665508791938 1103 32-33 3.6087949052965413 1103 34-35 3.39961251639037 1103 36-37 3.350238072336026 1103 38-39 3.21243505564113 1103 40-41 3.565933625805549 1103 42-43 3.2495754297507844 1103 44-45 3.312060746623743 1103 46-47 3.225597510574218 1103 48-49 3.3252363247608443 1103 50-51 3.1211932119271886 1103 52-53 3.063441207265658 1103 54-55 3.2361799601514463 1103 56-57 3.1516185523004197 1103 58-59 3.0129784938110795 1103 60-61 2.976846213752033 1103 62-63 2.94069730336167 1103 64-65 2.908548613905701 1103 66-67 2.9148343867188338 1103 68-69 3.129631020913912 1103 70-71 3.1168392710774704 1103 72-73 2.9083902227752425 1103 74-75 2.4573239926909523 1103 76-77 2.701409588051323 1103 78-79 2.7125506219193483 1103 80-81 2.6125413467029226 1103 82-83 2.6102759082533105 1103 84-85 2.56687137558124 1103 86-87 2.470147601800342 1103 88-89 2.4264588092029804 1103 90-91 2.485188839573386 1103 92-93 2.4135030093856997 1103 94-95 2.343564543020829 1103 96-97 2.3186459761831024 1103 98-99 2.4188483802964775 1103 100-101 1.8785894630175548 1104 1 3.3551078873363736 1104 2 6.907989498002415 1104 3 5.883396503136723 1104 4 5.6814426920395285 1104 5 5.974637885570118 1104 6 5.282348106864106 1104 7 5.711858347531088 1104 8 5.533957852900002 1104 9 5.469153095373141 1104 10-11 5.310509398998324 1104 12-13 5.730532762395917 1104 14-15 6.157455434343369 1104 16-17 5.626475671967977 1104 18-19 4.790211603209421 1104 20-21 4.560775090558138 1104 22-23 4.501563313140274 1104 24-25 4.378710313464367 1104 26-27 4.1316032962600175 1104 28-29 3.7796196154388255 1104 30-31 3.479483693865582 1104 32-33 3.5390309290787982 1104 34-35 3.260627388276589 1104 36-37 3.401017906789207 1104 38-39 3.1820744535951277 1104 40-41 3.5394924971807527 1104 42-43 3.2627768060340756 1104 44-45 3.1873372260350266 1104 46-47 3.118338893668543 1104 48-49 3.27206369086516 1104 50-51 3.0102933342424585 1104 52-53 2.9462350013009306 1104 54-55 3.051640379298732 1104 56-57 2.971596154578876 1104 58-59 2.9626176480103474 1104 60-61 2.8738142647648672 1104 62-63 2.726494016298556 1104 64-65 2.820297730302471 1104 66-67 2.7824737717807757 1104 68-69 3.0170462384418784 1104 70-71 2.967457857644863 1104 72-73 2.832061059025751 1104 74-75 2.5657156281127627 1104 76-77 2.7099949319247827 1104 78-79 2.7362536347124617 1104 80-81 2.594323326657033 1104 82-83 2.58222879885518 1104 84-85 2.4736248284235955 1104 86-87 2.3751046481447027 1104 88-89 2.3701825827944027 1104 90-91 2.449808196203609 1104 92-93 2.3120584441560936 1104 94-95 2.348898294534383 1104 96-97 2.331618654799815 1104 98-99 2.3898570053553705 1104 100-101 1.8321179627375273 1105 1 3.2023238226034927 1105 2 6.758517233117566 1105 3 5.619596880080959 1105 4 5.564674600168306 1105 5 5.777001362948333 1105 6 5.2244064903493665 1105 7 5.530398089350424 1105 8 5.521944827588136 1105 9 5.177097457655673 1105 10-11 4.857840377211506 1105 12-13 5.4149487811958465 1105 14-15 5.831450702672676 1105 16-17 5.188167647511715 1105 18-19 4.592375668488238 1105 20-21 4.548858687303991 1105 22-23 4.186532385328981 1105 24-25 3.8179705571068467 1105 26-27 3.6288176725290953 1105 28-29 3.104077366794236 1105 30-31 3.0793975653235037 1105 32-33 3.1509205069759503 1105 34-35 3.0134996295859438 1105 36-37 3.1747357892360597 1105 38-39 3.104365515903453 1105 40-41 3.1402589936513365 1105 42-43 3.1026139609788217 1105 44-45 3.028428683422419 1105 46-47 2.7462571067309725 1105 48-49 2.8121100749765944 1105 50-51 2.649651673281472 1105 52-53 2.626767355058117 1105 54-55 2.919926953437013 1105 56-57 2.9697225488581154 1105 58-59 2.5885382856755434 1105 60-61 2.660425211086654 1105 62-63 2.469434039395818 1105 64-65 2.417547237240143 1105 66-67 2.3877027078145012 1105 68-69 2.555727835274112 1105 70-71 2.4393281981394885 1105 72-73 2.4306763567535192 1105 74-75 2.181703518036059 1105 76-77 2.382269588153772 1105 78-79 2.3948407028947933 1105 80-81 2.1423751626204535 1105 82-83 2.1582305599674854 1105 84-85 2.0741305771497203 1105 86-87 1.8215695523264372 1105 88-89 1.9637360396952843 1105 90-91 1.8648558992076616 1105 92-93 1.8813251987309378 1105 94-95 1.8529404011248083 1105 96-97 1.8921998195762093 1105 98-99 1.8601426941613575 1105 100-101 1.1613128711236271 1106 1 3.2679263821758013 1106 2 6.660759502647469 1106 3 5.662277769346456 1106 4 5.566830675916702 1106 5 5.806494901346234 1106 6 5.224487658536702 1106 7 5.4295799474891275 1106 8 5.625168813636215 1106 9 5.217454745299225 1106 10-11 4.978093496573454 1106 12-13 5.467135640922741 1106 14-15 5.820502145694022 1106 16-17 5.319332935222047 1106 18-19 4.69836481708365 1106 20-21 4.535589868760653 1106 22-23 4.438538055754101 1106 24-25 4.29101948335007 1106 26-27 3.9551275619252806 1106 28-29 3.510033807473768 1106 30-31 3.2238711347334856 1106 32-33 3.4119090399695153 1106 34-35 3.103749097933786 1106 36-37 3.21717033011236 1106 38-39 3.1353896317756806 1106 40-41 3.385039811466079 1106 42-43 3.16190562782149 1106 44-45 2.9939025712535283 1106 46-47 2.835384868432776 1106 48-49 3.0193044117652548 1106 50-51 2.784519105169718 1106 52-53 2.8236847454314535 1106 54-55 3.0369835555624007 1106 56-57 2.9637088746644054 1106 58-59 2.7773679719299516 1106 60-61 2.8277318048576845 1106 62-63 2.745999918712794 1106 64-65 2.7269368063385855 1106 66-67 2.6140449863490822 1106 68-69 2.7628556192952374 1106 70-71 2.8645084271004144 1106 72-73 2.6628642980554282 1106 74-75 2.450379065727681 1106 76-77 2.5568793662469 1106 78-79 2.488134166082638 1106 80-81 2.3357782957008624 1106 82-83 2.3968301215280654 1106 84-85 2.31350704597018 1106 86-87 2.2521584234157146 1106 88-89 2.1144049136591434 1106 90-91 2.1967562749876635 1106 92-93 2.1568536071137565 1106 94-95 2.2380054866161423 1106 96-97 2.227101980358288 1106 98-99 2.2053665052998035 1106 100-101 1.6825808285322275 1107 1 2.767210656108716 1107 2 6.0633913662347325 1107 3 5.270093235398569 1107 4 4.5436118653558175 1107 5 4.264782767242075 1107 6 4.413061227296659 1107 7 1.2852522984015842 1107 8 3.714938179287607 1107 9 3.971722192444819 1107 10-11 4.628705656265797 1107 12-13 4.551122953862851 1107 14-15 4.689004874915803 1107 16-17 4.571516242784124 1107 18-19 4.13548343323022 1107 20-21 4.269454358860557 1107 22-23 3.6496875515491425 1107 24-25 3.4406422652762956 1107 26-27 3.251913554830324 1107 28-29 3.1165710100694852 1107 30-31 2.8610282202546955 1107 32-33 3.1318419912266577 1107 34-35 2.594388567653546 1107 36-37 2.5488731759107637 1107 38-39 2.610408345967201 1107 40-41 3.069932809567284 1107 42-43 2.8937116030788204 1107 44-45 2.9496090198268945 1107 46-47 2.7641607793214433 1107 48-49 2.9435339021625637 1107 50-51 2.650929125793592 1107 52-53 2.468835175673167 1107 54-55 2.7669387118392166 1107 56-57 2.761151898621989 1107 58-59 2.7208425903762965 1107 60-61 2.7177637568432544 1107 62-63 2.468702538972291 1107 64-65 2.4868753473898835 1107 66-67 2.6781670824238226 1107 68-69 2.873389633770394 1107 70-71 2.861913812296951 1107 72-73 2.6666453283491087 1107 74-75 2.440698870604578 1107 76-77 2.5921199132218895 1107 78-79 2.5135249165397973 1107 80-81 2.304491924107502 1107 82-83 2.306101290271222 1107 84-85 2.3851695740588887 1107 86-87 2.1871588563592326 1107 88-89 2.090217965694592 1107 90-91 2.0361688199124366 1107 92-93 2.123905577866015 1107 94-95 1.9567777343117605 1107 96-97 1.9737891800041645 1107 98-99 2.1251943436896 1107 100-101 1.5105066627530732 1108 1 2.885204768313532 1108 2 6.358176036682472 1108 3 5.237539343760655 1108 4 5.18504537391161 1108 5 5.42645180841874 1108 6 4.7791252600931315 1108 7 4.987892549709688 1108 8 4.90960829047625 1108 9 4.9508067896799695 1108 10-11 4.739162025582072 1108 12-13 5.131554520319618 1108 14-15 5.475048502202597 1108 16-17 5.010219983576928 1108 18-19 4.438353619889526 1108 20-21 4.0791319358243925 1108 22-23 4.001264726108822 1108 24-25 3.7486884183835514 1108 26-27 3.583785941480116 1108 28-29 3.227866231938023 1108 30-31 2.863528782514635 1108 32-33 3.1432904158208643 1108 34-35 2.8949906739203044 1108 36-37 2.9233166249708873 1108 38-39 2.753187263318665 1108 40-41 2.9675011310708683 1108 42-43 2.57481116889673 1108 44-45 2.804997253096019 1108 46-47 2.609096781829429 1108 48-49 2.6644587218731814 1108 50-51 2.3975896571803865 1108 52-53 2.432680404510311 1108 54-55 2.60103578423508 1108 56-57 2.536333658890065 1108 58-59 2.466858115465815 1108 60-61 2.471320410554874 1108 62-63 2.4059923616531655 1108 64-65 2.2779767491185225 1108 66-67 2.3463862635400936 1108 68-69 2.4696814245475736 1108 70-71 2.5395747375307742 1108 72-73 2.347521428879414 1108 74-75 2.1307560844555056 1108 76-77 2.2912973989007064 1108 78-79 2.2239112740383113 1108 80-81 2.054467987365875 1108 82-83 2.0215266870861477 1108 84-85 1.9246087630206752 1108 86-87 1.8710409606907241 1108 88-89 1.8552871500762222 1108 90-91 1.8960547366156497 1108 92-93 1.9597030560590305 1108 94-95 1.965054506475159 1108 96-97 1.9209282614542396 1108 98-99 2.003063433318605 1108 100-101 1.405103988229726 1109 1 2.898513710856612 1109 2 5.609269807782809 1109 3 5.103661182510077 1109 4 5.181162450984846 1109 5 5.206021747137761 1109 6 4.8226010853063315 1109 7 4.891076747856854 1109 8 4.90526127692403 1109 9 4.844869555484227 1109 10-11 4.772905189472699 1109 12-13 5.1333466673939725 1109 14-15 5.320014687261555 1109 16-17 5.002481712564592 1109 18-19 4.461567207306921 1109 20-21 3.7384036713721542 1109 22-23 3.919679343942711 1109 24-25 3.5586413328301845 1109 26-27 3.3539886071227727 1109 28-29 3.2043749824780363 1109 30-31 2.883961960645479 1109 32-33 2.938382174088993 1109 34-35 2.8171800241499056 1109 36-37 3.0425342188523885 1109 38-39 2.8437855960919904 1109 40-41 2.6631528758987812 1109 42-43 2.651409332067338 1109 44-45 2.804398424314755 1109 46-47 2.3693293359503045 1109 48-49 2.5917563010344438 1109 50-51 2.287458608683604 1109 52-53 2.355980226907473 1109 54-55 2.5986826425848193 1109 56-57 2.582085664522154 1109 58-59 2.325542664018588 1109 60-61 2.083878553885164 1109 62-63 2.2268372391712 1109 64-65 2.222522866656405 1109 66-67 2.2415774697993847 1109 68-69 2.4181942103521408 1109 70-71 2.239547313091986 1109 72-73 2.1006620039286155 1109 74-75 1.8750280664072179 1109 76-77 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72-73 -4.533301921412882 2314 74-75 -5.501136600122003 2314 76-77 -3.130693447805019 2314 78-79 -2.3997833820139647 2314 80-81 -5.348837570733529 2314 82-83 -3.7991189602133133 2314 84-85 -2.823220950873612 2314 86-87 -2.579013451673788 2314 88-89 -4.730384268750434 2314 90-91 -4.761384960418482 2314 92-93 -3.544224000071438 2314 94-95 -5.059897468788083 2314 96-97 -4.456298061614948 2314 98-99 -4.288642422117778 2314 100-101 -4.450810995632711 2315 1 -26.113958578300014 2315 2 -22.814865131976227 2315 3 -23.813562913875465 2315 4 -27.444264055039252 2315 5 -27.285872228765854 2315 6 -27.929148312284028 2315 7 -27.643940119613024 2315 8 -27.56452185560609 2315 9 -29.42560755294951 2315 10-11 -29.611528545467063 2315 12-13 -29.08786779043735 2315 14-15 -26.806151114537144 2315 16-17 -29.803465275387214 2315 18-19 -30.432071346909943 2315 20-21 -30.761432473206156 2315 22-23 -30.750421860876564 2315 24-25 -27.465796409886405 2315 26-27 -27.78698078318689 2315 28-29 -27.784481390705306 2315 30-31 -28.755481372190054 2315 32-33 -23.321762849409197 2315 34-35 -21.231894989344973 2315 36-37 -27.810889477011326 2315 38-39 -26.352829419666634 2315 40-41 -27.576903821884663 2315 42-43 -24.73050397244911 2315 44-45 -17.11317462831401 2315 46-47 -18.86645718952978 2315 48-49 -19.995821271189214 2315 50-51 -15.031654890837643 2315 52-53 -14.515918889424906 2315 54-55 -14.54288803483437 2315 56-57 -22.85637766142202 2315 58-59 -23.859148794732413 2315 60-61 -18.735813354778504 2315 62-63 -17.259573302656797 2315 64-65 -15.04968882890362 2315 66-67 -14.080512831214929 2315 68-69 -13.67475434436809 2315 70-71 -15.11209692496404 2315 72-73 -16.691367379389575 2315 74-75 -15.055772522166873 2315 76-77 -12.939777304202723 2315 78-79 -12.198186741581043 2315 80-81 -11.659502878012717 2315 82-83 -13.44596186711061 2315 84-85 -12.006784722658516 2315 86-87 -10.799960157749442 2315 88-89 -10.481644491820974 2315 90-91 -10.23326287142321 2315 92-93 -11.408414947195133 2315 94-95 -10.800240380730399 2315 96-97 -10.136838017565593 2315 98-99 -9.679889481380421 2315 100-101 -8.029394017255429 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2595985.0 3 49436.0 4 55504.0 5 81326.0 6 132718.0 7 205510.0 8 278988.0 9 363671.0 10 388136.0 11 359995.0 12 318452.0 13 302208.0 14 310628.0 15 350230.0 16 420700.0 17 466050.0 18 496095.0 19 532353.0 20 581104.0 21 640255.0 22 705343.0 23 789801.0 24 900098.0 25 1021112.0 26 1164618.0 27 1319660.0 28 1490867.0 29 1677943.0 30 1878383.0 31 2110849.0 32 2449590.0 33 2930579.0 34 3617308.0 35 4676222.0 36 6342788.0 37 8793638.0 38 8345852.0 39 1529464.0 40 820.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.450313980651487 15.4060524404438 12.635627514639555 42.50800606426516 2 18.25098952398463 18.1314261421967 49.922951172761806 13.69463316105687 3 20.03737636324183 24.49796284523604 33.102212724478434 22.362448067043694 4 21.16203910659816 31.359003921392176 28.742326606023532 18.73663036598613 5 20.163210644609567 31.866063648584518 32.39701559047219 15.57371011633372 6 15.59332577827393 35.1889611027423 32.89676540441659 16.320947714567176 7 13.473483966570804 17.998396456683015 49.78676262421449 18.741356952531692 8 15.293624587928395 21.348702076786303 42.512050918282334 20.845622417002964 9 18.206257611158634 21.5408449129603 38.863996942567184 21.388900533313883 10-11 20.889248589840392 32.67185952536714 28.17413446499117 18.2647574198013 12-13 18.721263294681968 27.31451727736956 35.64468930452392 18.31953012342455 14-15 19.59767447867207 26.888259790187153 35.34093153310403 18.17313419803675 16-17 20.453930053295764 26.99103686194107 33.35005953469717 19.204973550065997 18-19 20.578103573761947 28.099309876002838 32.032341143867306 19.290245406367912 20-21 20.042929691758886 29.21399676642954 31.798344779500848 18.944728762310728 22-23 20.053400303130456 28.79741861488918 33.53636806573763 17.612813016242733 24-25 19.71243491648152 29.190951847731643 32.97744000263319 18.119173233153653 26-27 20.4071022246723 28.79645797973902 31.963994666539282 18.832445129049397 28-29 20.353360168234428 29.183098253692062 30.452869196722034 20.01067238135148 30-31 19.624445585872678 28.90149602549276 31.299693028232383 20.17436536040218 32-33 19.807785721109212 28.57114318812503 31.452029263501817 20.16904182726394 34-35 18.75220238533271 29.64833157307625 30.387540477901926 21.211925563689114 36-37 19.236023358466507 28.89516953394415 31.00906110619428 20.859746001395063 38-39 19.9079571385261 28.096513866196222 31.589442464069904 20.406086531207773 40-41 20.716986772811488 28.61182286866672 30.705413570720086 19.965776787801705 42-43 20.49186164212062 29.51017262072818 30.49251136674726 19.50545437040394 44-45 20.158359229830218 29.302945528979574 30.651255623331082 19.88743961785913 46-47 20.143428895955097 29.05935953324156 30.424802389501465 20.372409181301872 48-49 20.832509564599256 29.08969047195264 29.648811563796528 20.428988399651573 50-51 20.281673645159902 29.13976654565409 30.40411752806541 20.1744422811206 52-53 19.890512368490825 29.53543245995482 30.936962148496445 19.637093023057908 54-55 19.900318603158095 29.677800183827348 30.633453469511412 19.78842774350315 56-57 20.61983801768586 28.74993001413349 30.424795478599375 20.20543648958127 58-59 20.616645169097314 28.738416203719495 29.88803443684297 20.75690419034022 60-61 21.279301888856043 28.881582303924198 29.792063912418044 20.047051894801708 62-63 19.274422584507906 31.114599148286793 29.74869188803524 19.862286379170058 64-65 18.945813477198485 29.996184260515523 30.544864994566595 20.513137267719394 66-67 19.468073918706914 28.58526142456579 31.006061818145586 20.94060283858171 68-69 20.56825994629603 29.48896694039707 29.287515780674784 20.655257332632118 70-71 19.873662362489405 30.184225880569876 29.47972348538802 20.462388271552694 72-73 19.20938276476967 30.355900559921285 30.108452259016342 20.3262644162927 74-75 19.749961659787953 28.97199528726481 30.871340961380504 20.40670209156673 76-77 20.51420340123202 28.99076254756716 29.626600819802622 20.8684332313982 78-79 20.19653511308573 30.28022595555025 28.791922680783642 20.731316250580374 80-81 20.038476418413925 29.61792568095817 30.80279967811278 19.540798222515125 82-83 19.872177625215315 29.72342823569371 30.321043566006033 20.083350573084942 84-85 19.876110306310917 30.08707334682233 29.476203492641467 20.56061285422528 86-87 20.39641511083725 29.901419762174715 29.316234888596863 20.385930238391175 88-89 20.20661300850249 29.460968017274496 29.932485253910002 20.399933720313008 90-91 20.01485824921876 30.14170335568312 29.350448143476587 20.492990251621535 92-93 19.57817918210343 30.223215649115552 29.743700080780695 20.454905088000324 94-95 19.37937879023577 29.986658141004973 29.38843891959562 21.245524149163632 96-97 19.65300786338267 29.402439432510143 29.742586937139592 21.201965766967596 98-99 20.133429613841468 29.595865723485577 29.561862455727038 20.708842206945917 100-101 20.673532788202014 30.075105717128025 28.253303893567622 20.99805760110234 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7914.0 1 8454.0 2 7944.0 3 7408.5 4 8601.0 5 10425.5 6 12395.5 7 14517.5 8 17651.0 9 21638.5 10 26470.0 11 32808.0 12 41808.0 13 53307.0 14 67724.0 15 85570.5 16 105787.5 17 128805.5 18 155180.0 19 185803.0 20 222743.0 21 268430.0 22 320970.5 23 384706.0 24 461054.5 25 548450.5 26 644314.5 27 762421.5 28 911475.5 29 1071544.5 30 1263092.5 31 1519509.0 32 1948429.5 33 2344557.0 34 2663426.0 35 3066474.5 36 3387165.0 37 3375304.0 38 3050045.5 39 2686435.5 40 2442756.5 41 2259366.0 42 2183405.5 43 2242573.0 44 2199692.0 45 2123407.0 46 2060675.5 47 1897708.5 48 1707368.0 49 1543007.0 50 1352420.0 51 1162413.0 52 1008066.5 53 871637.0 54 743389.0 55 630871.0 56 529072.0 57 433221.5 58 348194.0 59 271582.0 60 203832.5 61 149383.0 62 108280.0 63 76918.0 64 53393.0 65 37639.0 66 26655.5 67 18878.5 68 13178.5 69 9648.5 70 8297.5 71 8251.5 72 7419.5 73 7678.5 74 7502.5 75 5455.5 76 4617.0 77 4184.5 78 3685.0 79 3198.5 80 2547.0 81 1841.0 82 1347.0 83 1028.0 84 626.5 85 320.5 86 221.5 87 164.0 88 115.5 89 77.5 90 63.5 91 55.5 92 37.0 93 30.5 94 28.5 95 17.5 96 10.0 97 7.0 98 4.5 99 5.0 100 4.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 4.523752148748236 2 17.5027312644292 3 10.541740430075814 4 8.719307566885137 5 11.929570683485172 6 7.611536677675231 7 9.531780344682794 8 10.014729635270985 9 6.151519328313732 10-11 5.831016961899127 12-13 8.468436847844538 14-15 8.943295395401401 16-17 7.2046962436916635 18-19 4.883186992629941 20-21 5.678830563441883 22-23 5.3121471785433165 24-25 2.557881239923758 26-27 3.2910246860947456 28-29 3.3973308525017663 30-31 1.2895942282231323 32-33 1.9866540152871037 34-35 1.8474904333020588 36-37 1.901425973269497 38-39 1.7874806555179665 40-41 3.0989498861618117 42-43 1.602100619934849 44-45 1.9573854351033986 46-47 1.0686060233200299 48-49 1.1885860234119965 50-51 0.4662593518416593 52-53 0.7426235753044548 54-55 0.8086326003148715 56-57 1.3415544665969579 58-59 0.0830187038563738 60-61 0.5194614673542309 62-63 1.2194664892515659 64-65 0.47177569262916175 66-67 0.004063501108929534 68-69 0.46800556130217874 70-71 0.03281044345001611 72-73 6.477209230619782E-4 74-75 6.328876194804062E-4 76-77 0.0022777361721925036 78-79 0.0035748261631588568 80-81 3.8319367585727714E-4 82-83 9.081278081606869E-4 84-85 2.3733285730515233E-4 86-87 0.46561657535312456 88-89 0.27532754035692786 90-91 3.947306897540554E-4 92-93 2.1096253982680208E-4 94-95 3.3045304090057663E-4 96-97 7.49081830869387E-4 98-99 0.0059984231539034856 100-101 5.537766670453554E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6.0674279E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.979380265491855 #Duplication Level Percentage of deduplicated Percentage of total 1 70.98840610962226 23.411536395303976 2 11.48029668906175 7.572261401384692 3 4.916941941632599 4.86473094109342 4 2.922881713959686 3.855793100629163 5 1.900672685286107 3.134150362414202 6 1.2946364590314219 2.561778485278028 7 0.9490248546217637 2.190877609338199 8 0.7543346993866519 1.9901992718822301 9 0.5757994530562792 1.7090558207104731 >10 3.744701598422158 23.1821184146835 >50 0.3002781702198404 6.810337754525711 >100 0.15522453015408574 9.3631984272977 >500 0.010202725948659884 2.2797103206028284 >1k 0.005676653265913787 3.4894016420328016 >5k 5.903152298993197E-4 1.3648116673395756 >10k+ 3.3140110103338543E-4 2.220038385483556 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 89927 0.14821272124222523 No Hit GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG 70353 0.11595193409714849 No Hit AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA 62199 0.10251296105224424 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.008520908835191927 0.0 0.0 3.296289684793782E-6 0.0 2 0.00855057544235507 0.0 0.0 1.483330358157202E-5 0.0 3 0.008576945759833421 0.0 0.0 1.648144842396891E-5 0.0 4 0.008960963508111897 0.0 0.0 2.6370317478350256E-5 0.0 5 0.00905490776412852 0.0 0.0 2.6370317478350256E-5 0.0 6 0.009173574192781095 0.0 0.0 2.966660716314404E-5 0.0 7 0.00924609256584656 0.0 0.0 3.131475200554093E-5 0.0 8 0.009836128419424646 3.296289684793782E-6 0.0 3.131475200554093E-5 0.0 9 0.009976220731028381 3.296289684793782E-6 0.0 4.285176590231917E-5 0.0 10-11 0.01044923830079629 3.296289684793782E-6 0.0 4.69721280083114E-5 0.0 12-13 0.011148051713972571 3.296289684793782E-6 0.0 5.686099706269274E-5 0.0 14-15 0.012053707304869665 3.296289684793782E-6 0.0 5.768506948389119E-5 0.0 16-17 0.01322224199812906 3.296289684793782E-6 0.0 5.768506948389119E-5 0.0 18-19 0.014313313883795802 3.296289684793782E-6 0.0 6.098135916868497E-5 0.0 20-21 0.015468663418316021 3.296289684793782E-6 0.0 6.510172127467719E-5 0.0 22-23 0.01668829060168972 3.296289684793782E-6 0.0 6.592579369587564E-5 0.0 24-25 0.017837047556840353 3.296289684793782E-6 0.0 7.74628075926539E-5 0.0 26-27 0.019050082160844466 3.296289684793782E-6 0.0 8.48794593834399E-5 0.0 28-29 0.02043534790087905 4.944434527190673E-6 0.0 9.064796633182901E-5 0.0 30-31 0.02182803029270443 6.592579369587564E-6 0.0 9.22961111742259E-5 0.0 32-33 0.023370693865187916 9.0647966331829E-6 0.0 1.030090526498057E-4 0.0 34-35 0.02516222730887334 9.888869054381346E-6 8.240724211984455E-7 1.0630534233459948E-4 0.0 36-37 0.027120223381640843 1.1537013896778237E-5 1.648144842396891E-6 1.0795348717699638E-4 0.0 38-39 0.029510033403116337 1.4009231160373574E-5 1.648144842396891E-6 1.104257044405917E-4 0.0 40-41 0.03210338931262784 1.7305520845167354E-5 1.648144842396891E-6 1.1124977686179015E-4 0.0 42-43 0.03495303174513207 1.81295932663658E-5 1.648144842396891E-6 1.1289792170418704E-4 0.0 44-45 0.03816526604296361 1.81295932663658E-5 1.648144842396891E-6 1.1866642865257615E-4 0.0 46-47 0.0418274438827695 1.9777738108762692E-5 1.648144842396891E-6 1.3185158739175128E-4 0.0 48-49 0.04597417630624008 2.0601810529961136E-5 1.648144842396891E-6 1.3597194949774354E-4 0.0 50-51 0.05071259272813114 2.1425882951159583E-5 1.648144842396891E-6 1.44212673709728E-4 1.648144842396891E-6 52-53 0.055942156313056476 2.1425882951159583E-5 1.648144842396891E-6 1.4915710823691864E-4 2.4722172635953365E-6 54-55 0.061589524615529426 2.1425882951159583E-5 1.648144842396891E-6 1.5080525307931554E-4 3.296289684793782E-6 56-57 0.06787307682716756 2.1425882951159583E-5 1.648144842396891E-6 1.532774703429109E-4 3.296289684793782E-6 58-59 0.07471864643006307 2.3074027793556474E-5 1.648144842396891E-6 1.681107739244829E-4 3.296289684793782E-6 60-61 0.08245833460995886 2.3074027793556474E-5 1.648144842396891E-6 1.8376814992725334E-4 3.296289684793782E-6 62-63 0.09094957683798764 2.3898100214754918E-5 1.648144842396891E-6 1.8871258445444405E-4 4.1203621059922276E-6 64-65 0.1001956694038342 2.4722172635953365E-5 1.648144842396891E-6 1.936570189816347E-4 4.944434527190673E-6 66-67 0.11019496416265614 2.4722172635953365E-5 1.648144842396891E-6 2.0189774319361915E-4 4.944434527190673E-6 68-69 0.12147651560886286 2.4722172635953365E-5 1.648144842396891E-6 2.0931439498440518E-4 4.944434527190673E-6 70-71 0.1347564426764758 2.5546245057151813E-5 1.648144842396891E-6 2.134347570903974E-4 4.944434527190673E-6 72-73 0.14944306136707453 2.8018462320747147E-5 1.648144842396891E-6 2.1590697435399273E-4 4.944434527190673E-6 74-75 0.16633654600164266 2.966660716314404E-5 1.648144842396891E-6 2.224995537235803E-4 4.944434527190673E-6 76-77 0.1855094149532457 2.966660716314404E-5 1.648144842396891E-6 2.2661991582957253E-4 4.944434527190673E-6 78-79 0.20831067477538545 3.131475200554093E-5 1.648144842396891E-6 2.3074027793556476E-4 4.944434527190673E-6 80-81 0.23597725817228088 3.213882442673938E-5 1.648144842396891E-6 2.3403656762035853E-4 4.944434527190673E-6 82-83 0.2691057935768796 3.378696926913627E-5 1.648144842396891E-6 2.42277291832343E-4 4.944434527190673E-6 84-85 0.31047917355556875 3.543511411153316E-5 1.648144842396891E-6 2.546383781503197E-4 4.944434527190673E-6 86-87 0.3633063361164951 3.7083258953930046E-5 1.648144842396891E-6 2.6205502994110566E-4 4.944434527190673E-6 88-89 0.4266124035853809 3.79073313751285E-5 1.648144842396891E-6 2.6864760931069327E-4 4.944434527190673E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 58600 0.0 43.918774 1 GCGTGTT 64485 0.0 36.411522 2 GTTTTTT 525705 0.0 33.914566 1 CTGGAAT 141530 0.0 27.70403 1 CGTGTTT 80465 0.0 27.67763 3 CGCGTGC 4340 0.0 26.482471 1 CGCGTTT 4440 0.0 20.436329 1 CGGCGAG 7025 0.0 19.570215 1 CCCGCGT 4070 0.0 19.051388 1 CCGGCAT 7155 0.0 18.29234 1 CTCGCGT 20740 0.0 18.00375 1 CGCGTAT 3725 0.0 17.715643 1 CGCGGTT 2225 0.0 17.548107 1 CCGAAAC 5365 0.0 17.220331 1 TATGGCG 209620 0.0 17.08267 7 CGGCAAG 6910 0.0 16.951319 1 ATGGCGA 212370 0.0 16.783113 8 CCGATCT 3705 0.0 16.614838 2 TGGCGAG 212725 0.0 16.55708 9 GAATATG 263720 0.0 16.013016 4 >>END_MODULE