FastQCFastQC Report
Fri 27 May 2016
SRR1233070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233070_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60674279
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1115240.18380770540347088No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA730000.12031457349497304No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG701390.11559923110087554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCA94550.053.7206421
ATTCGAC155150.046.3787041
CCGATAG69600.045.3039171
CGCGTTT61900.039.107371
TTCGTAT127600.038.949071
CGATAGT82000.038.9163862
GCGGGTT34600.037.662221
CGCGTGT657050.036.4879761
TTCGACT217600.034.240582
GCGTGTT686050.032.7125972
GCGCGTT33200.028.363421
CGGGTTT108200.027.4276641
CGTCTCA211700.027.1604481
CAATACG125100.027.027014
CGTGTTT828300.026.4294783
GTAAACT366250.026.2946512
CGGGAAT81400.025.2400861
CTGGAAT1586500.024.87211
ATGACTC326550.023.558123
CCGAAAC51150.022.9658641
Produced by FastQC (version 0.11.5)