FastQCFastQC Report
Fri 27 May 2016
SRR1233069_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233069_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97937573
Sequences flagged as poor quality0
Sequence length100
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC2020270.20628140335885187No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG1935420.1976177212396309No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA1594570.16281493926748625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT988900.080.9678341
GCGTGTT1156850.065.113362
CGTGTTT1726100.044.2117463
CGCGAGT48800.042.4609571
CGCGTAT38050.039.5050431
CGGCGAG127400.039.3449971
CCGCGTG56600.038.3023721
CTGGAAT3268800.035.6798171
CGCGTGC54300.035.513181
GTTTTTT12929650.032.464521
CGCGCGT36100.032.3490071
CGCGGGT58650.031.6538981
CGCGTGG55650.028.4100651
CTCGTGT142250.026.9860461
CGCGTGA43850.024.3099421
GCGCGTT43650.023.5982462
CGCGTTT114050.023.2617261
CTGGCGA136050.022.3613831
GGAAAAC6152200.020.6015931
GTGTTTT3496300.020.3293614
Produced by FastQC (version 0.11.5)