##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233069_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 97937573 Sequences flagged as poor quality 0 Sequence length 100 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.45692924205912 31.0 25.0 33.0 10.0 34.0 2 21.97278051805511 30.0 10.0 33.0 2.0 34.0 3 23.166980460093697 30.0 16.0 31.0 2.0 34.0 4 27.30327927362464 33.0 19.0 37.0 10.0 37.0 5 27.597881233997906 33.0 19.0 37.0 10.0 37.0 6 28.378146362683502 35.0 23.0 37.0 10.0 37.0 7 24.74420732276059 33.0 10.0 37.0 2.0 37.0 8 25.87684250660367 33.0 17.0 37.0 2.0 37.0 9 27.27873359696181 33.0 17.0 39.0 2.0 39.0 10-11 28.791555147073126 34.5 20.0 39.0 10.0 39.0 12-13 28.22286997555065 34.5 21.0 39.0 2.0 39.0 14-15 28.255938739670423 34.0 17.0 39.0 2.0 41.0 16-17 29.241317691219486 35.5 21.0 39.0 2.0 41.0 18-19 29.67852745340136 36.0 24.5 39.5 2.0 41.0 20-21 29.49467494462008 36.0 22.0 40.0 2.0 41.0 22-23 29.087537833922024 35.0 21.0 39.0 2.0 41.0 24-25 29.2522968483199 35.5 24.0 39.0 2.0 41.0 26-27 28.973868435559456 35.5 22.0 39.0 2.0 41.0 28-29 28.97579575511842 35.5 23.5 39.0 2.0 41.0 30-31 28.896316304468765 36.0 23.0 40.0 2.0 41.0 32-33 28.803102007643176 36.0 23.0 40.0 2.0 41.0 34-35 28.740863789834776 36.0 22.5 40.0 2.0 41.0 36-37 28.534647346223295 35.5 20.0 40.0 2.0 41.0 38-39 28.627402462791274 35.0 21.5 40.0 2.0 41.0 40-41 28.52189990964959 35.5 21.0 40.0 2.0 41.0 42-43 28.136754455820544 35.0 17.5 40.0 2.0 41.0 44-45 27.664734958257544 35.0 15.0 39.0 2.0 41.0 46-47 27.59023652750717 35.0 15.0 39.0 2.0 41.0 48-49 27.6189243733863 35.0 15.0 39.0 2.0 41.0 50-51 27.00620017406394 34.0 14.5 39.0 2.0 40.0 52-53 26.862357131312613 33.5 13.5 38.5 2.0 40.0 54-55 27.439497709423534 35.0 14.0 39.0 2.0 41.0 56-57 27.484110868256863 35.0 13.5 40.0 2.0 41.0 58-59 27.310340858661057 35.0 13.0 39.0 2.0 41.0 60-61 27.20195690371049 35.0 12.5 39.0 2.0 41.0 62-63 26.85576665249812 34.0 8.0 39.0 2.0 41.0 64-65 26.605385412195176 34.0 8.0 39.0 2.0 41.0 66-67 26.473390743509643 34.0 8.0 39.0 2.0 41.0 68-69 26.2226017281437 34.0 7.5 38.5 2.0 40.0 70-71 25.80664059339106 34.0 7.0 38.0 2.0 40.0 72-73 25.408849022631998 33.0 2.0 37.0 2.0 40.0 74-75 25.08298927317711 32.5 2.0 37.0 2.0 39.0 76-77 24.83535166324777 32.0 2.0 36.5 2.0 39.0 78-79 24.483515749364138 32.0 2.0 36.0 2.0 39.0 80-81 24.04350326304288 32.0 2.0 35.5 2.0 37.5 82-83 23.728774399994577 31.5 2.0 35.0 2.0 37.0 84-85 23.47450671970399 31.5 2.0 35.0 2.0 37.0 86-87 23.0834220743861 31.0 2.0 35.0 2.0 36.0 88-89 22.65288964226222 31.0 2.0 35.0 2.0 36.0 90-91 22.42756220332313 31.0 2.0 35.0 2.0 36.0 92-93 22.123215974526957 31.0 2.0 35.0 2.0 35.0 94-95 21.790928033309545 31.0 2.0 35.0 2.0 35.0 96-97 21.49411294886795 31.0 2.0 35.0 2.0 35.0 98-99 21.096952907950865 31.0 2.0 35.0 2.0 35.0 100 19.90989947239146 29.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.30867213785899494 1101 2 5.205078254872799 1101 3 3.858424094001645 1101 4 2.1911348719404664 1101 5 2.39234651666159 1101 6 1.5301645990880317 1101 7 4.740326347048658 1101 8 3.8394162892850225 1101 9 3.316706190320339 1101 10-11 0.1119861087977938 1101 12-13 1.8390343775103588 1101 14-15 2.602931095526184 1101 16-17 1.6713670069686906 1101 18-19 0.8985204164434748 1101 20-21 0.8956578920765281 1101 22-23 0.9592796432983945 1101 24-25 0.682813475777543 1101 26-27 0.42696487493961044 1101 28-29 0.13164114182317732 1101 30-31 0.011151442286035262 1101 32-33 -0.017963600542842073 1101 34-35 0.11229182596949627 1101 36-37 0.27142877702114987 1101 38-39 -0.04875761693137193 1101 40-41 -0.10039665095150596 1101 42-43 0.03470066351111001 1101 44-45 0.24024685138561708 1101 46-47 0.15855003619238772 1101 48-49 0.16708384566550194 1101 50-51 -0.03795392737347214 1101 52-53 0.22179106343758193 1101 54-55 0.01117306444573174 1101 56-57 -0.03664875074569096 1101 58-59 -0.04644587406780332 1101 60-61 -0.2780852754089018 1101 62-63 -0.24506387614026792 1101 64-65 -0.03666369640460587 1101 66-67 -0.20988760336495815 1101 68-69 -0.24304779190651615 1101 70-71 -0.17844759431984158 1101 72-73 -0.05455754117117806 1101 74-75 -0.17210283020176576 1101 76-77 -0.22619716429103676 1101 78-79 -0.3231188933769573 1101 80-81 -0.20668974306182264 1101 82-83 -0.2111103657553386 1101 84-85 -0.3218653580448674 1101 86-87 -0.2565696147199148 1101 88-89 -0.168745295350476 1101 90-91 -0.2458896967660742 1101 92-93 -0.34727737989783236 1101 94-95 -0.3688884959508272 1101 96-97 -0.5323997044574789 1101 98-99 -0.578081514782518 1101 100 -0.5014795967268206 1102 1 0.8620762515589817 1102 2 5.792503388153808 1102 3 3.704940616068221 1102 4 2.8581702800505653 1102 5 2.1318760311119824 1102 6 1.5336359516448574 1102 7 5.414324108181379 1102 8 4.536641181248083 1102 9 3.3811332671625003 1102 10-11 2.2981137967154055 1102 12-13 2.4408928826855387 1102 14-15 3.0653525632362566 1102 16-17 1.562122926648648 1102 18-19 1.1880070558871942 1102 20-21 1.2380035488104575 1102 22-23 1.433819004518348 1102 24-25 0.9666017440267005 1102 26-27 0.9890076566411459 1102 28-29 0.6615949584299479 1102 30-31 0.3721109958911839 1102 32-33 0.5065541689643283 1102 34-35 0.6186872335250939 1102 36-37 0.6693549600566726 1102 38-39 0.39696952055475165 1102 40-41 0.5735524125089917 1102 42-43 0.5786038138244294 1102 44-45 0.9966851362105089 1102 46-47 0.31844915076188585 1102 48-49 0.5890467007064579 1102 50-51 0.7502888138105206 1102 52-53 0.8740557561350677 1102 54-55 0.5152197672865384 1102 56-57 0.6034493109455532 1102 58-59 0.5352549352569014 1102 60-61 0.41108345180697725 1102 62-63 0.39472257960176194 1102 64-65 0.5212376624100408 1102 66-67 0.33029728974248584 1102 68-69 0.3088297646406488 1102 70-71 0.3068692997452622 1102 72-73 0.38043712348649095 1102 74-75 0.5181338647762068 1102 76-77 0.34697797721209866 1102 78-79 0.22074193142086784 1102 80-81 0.32826936149798414 1102 82-83 0.38927059174676515 1102 84-85 0.2793862909062952 1102 86-87 0.3799225043896719 1102 88-89 0.5240374532573107 1102 90-91 0.5242665827795996 1102 92-93 0.5001092853308258 1102 94-95 0.49039187719310107 1102 96-97 0.43120879953834645 1102 98-99 0.46055026826312684 1102 100 0.5082145328325218 1103 1 0.2546825714047074 1103 2 4.321629624339106 1103 3 3.7496117980846932 1103 4 2.3885563158558654 1103 5 2.3287659979100894 1103 6 1.756882137551223 1103 7 4.955752491381485 1103 8 3.8746584497245173 1103 9 0.4696659381437094 1103 10-11 1.016544775276067 1103 12-13 1.8180252153403629 1103 14-15 2.8141343104236185 1103 16-17 1.8464355771656855 1103 18-19 1.3652475979576408 1103 20-21 1.4309747909450259 1103 22-23 1.1952276049609267 1103 24-25 1.1603765388918745 1103 26-27 1.0592685765817755 1103 28-29 0.7491618567728082 1103 30-31 0.7605823423843034 1103 32-33 0.7161834021070987 1103 34-35 0.8191959805983551 1103 36-37 0.6819011600459639 1103 38-39 0.4971805458681722 1103 40-41 0.681856559048807 1103 42-43 0.9007150523366931 1103 44-45 1.017551861782426 1103 46-47 0.2147201252740487 1103 48-49 0.4277666764976651 1103 50-51 0.4667959635813048 1103 52-53 0.5542534711337233 1103 54-55 0.5299859953565367 1103 56-57 0.385740008550556 1103 58-59 0.35224833202460815 1103 60-61 0.3818723432400013 1103 62-63 0.7613407304086479 1103 64-65 0.5565105367409089 1103 66-67 0.502002925996667 1103 68-69 0.3503134791521667 1103 70-71 0.19323667567883263 1103 72-73 0.31905342325694974 1103 74-75 0.2618813079615663 1103 76-77 0.28322016496921876 1103 78-79 0.5642547069579571 1103 80-81 0.4895046810830088 1103 82-83 0.5719110772206726 1103 84-85 0.7018648153741367 1103 86-87 0.73007617679162 1103 88-89 0.788997223759317 1103 90-91 0.749741091388799 1103 92-93 0.5644968466622586 1103 94-95 0.7013908170609611 1103 96-97 0.7000270126219732 1103 98-99 0.7274996429331324 1103 100 0.6029259221829975 1104 1 0.9901942785676177 1104 2 1.7244724352287832 1104 3 2.3822478861566516 1104 4 2.9011575472603575 1104 5 1.9892936119614504 1104 6 2.5675716325781366 1104 7 4.531419243492838 1104 8 3.353083056463632 1104 9 2.501605556867368 1104 10-11 1.0621972749296447 1104 12-13 2.0661079916216636 1104 14-15 1.6621410375690076 1104 16-17 -0.3253763114647725 1104 18-19 -0.355821546052038 1104 20-21 1.428736884266769 1104 22-23 1.0302289419231556 1104 24-25 -0.1987780268851651 1104 26-27 0.17835544706067807 1104 28-29 1.2069512504074886 1104 30-31 1.111863261906894 1104 32-33 0.7743186098445314 1104 34-35 1.39676150087573 1104 36-37 1.2952586581853147 1104 38-39 1.1755294799404261 1104 40-41 1.3735894012630325 1104 42-43 1.2507370479831863 1104 44-45 1.413838129694156 1104 46-47 1.7063218837650922 1104 48-49 1.6476174283705483 1104 50-51 1.751376470501917 1104 52-53 1.894551981682504 1104 54-55 1.0533358276839202 1104 56-57 1.1119752933095306 1104 58-59 1.1922529256838814 1104 60-61 1.5588325947586128 1104 62-63 1.703701702101739 1104 64-65 1.9560105320408105 1104 66-67 2.0810826069633315 1104 68-69 1.66354126372104 1104 70-71 1.9566599699082943 1104 72-73 2.1631069263320946 1104 74-75 1.7094776930522926 1104 76-77 1.7791226069365145 1104 78-79 1.98409757421895 1104 80-81 2.194116193048611 1104 82-83 2.120466899699043 1104 84-85 2.037177920967082 1104 86-87 2.0126272340383515 1104 88-89 2.138453083984256 1104 90-91 1.9496650730437004 1104 92-93 1.951592321845233 1104 94-95 1.956255918295188 1104 96-97 1.9290786840922252 1104 98-99 1.883128232413405 1104 100 1.8063649438802685 1105 1 -8.855335538150868 1105 2 -19.94686820440578 1105 3 -21.15766717657697 1105 4 -12.20971854985945 1105 5 -12.54812060647542 1105 6 -7.385202259902254 1105 7 -22.811719436889803 1105 8 -12.4514343550847 1105 9 -25.32832301585941 1105 10-11 -13.894415971368135 1105 12-13 -7.403616226845553 1105 14-15 -21.113689417036944 1105 16-17 -10.931631679193899 1105 18-19 -4.443248705476819 1105 20-21 -4.948037670788921 1105 22-23 -5.359631554932111 1105 24-25 -5.020448863242528 1105 26-27 -3.397574905117313 1105 28-29 -3.7398115648861783 1105 30-31 -5.12212179205801 1105 32-33 -4.46686645819727 1105 34-35 -4.816641258429957 1105 36-37 -5.217347037964242 1105 38-39 -3.9791698891235363 1105 40-41 -2.651836307805194 1105 42-43 -4.265103167673274 1105 44-45 -4.742068241897247 1105 46-47 -5.7947891735542 1105 48-49 -4.688917671548079 1105 50-51 -5.639577573543406 1105 52-53 -5.742576921585229 1105 54-55 -5.145253520447916 1105 56-57 -4.571661919056261 1105 58-59 -3.3884001191066204 1105 60-61 -3.5173021772795927 1105 62-63 -2.8828773146197655 1105 64-65 -3.858181346614142 1105 66-67 -4.4341705008794925 1105 68-69 -2.925594083593726 1105 70-71 -4.031954857832133 1105 72-73 -3.8486805971100644 1105 74-75 -3.700824440513024 1105 76-77 -2.9640759497142497 1105 78-79 -3.496343016760054 1105 80-81 -2.8443935379336303 1105 82-83 -3.111936969403562 1105 84-85 -4.393109887595138 1105 86-87 -3.9332396279573842 1105 88-89 -5.769868490867196 1105 90-91 -4.805851098481508 1105 92-93 -6.4825962042198775 1105 94-95 -4.097290460186819 1105 96-97 -3.4529411000840184 1105 98-99 -3.093197707343599 1105 100 -2.959179544547336 1201 1 1.9219126413803131 1201 2 6.784805427250362 1201 3 5.085142705975819 1201 4 4.4733059650347435 1201 5 4.22470766632118 1201 6 1.663281512696468 1201 7 6.559900520927698 1201 8 5.5241055273810815 1201 9 5.301812846044655 1201 10-11 3.808794178380225 1201 12-13 4.1110017445635805 1201 14-15 4.930362791550028 1201 16-17 3.7025207239880444 1201 18-19 3.1562193691316054 1201 20-21 3.13802940733121 1201 22-23 3.221235810903316 1201 24-25 2.9231585832438896 1201 26-27 2.842399971007705 1201 28-29 2.628743601383544 1201 30-31 2.4937576345726953 1201 32-33 2.3839700240863024 1201 34-35 2.1762768493670137 1201 36-37 2.395354318776267 1201 38-39 2.1262474196066847 1201 40-41 1.874264347123269 1201 42-43 2.343349119016125 1201 44-45 2.59428373003227 1201 46-47 2.5581016842381956 1201 48-49 2.373235985452599 1201 50-51 2.2980358916830497 1201 52-53 2.5407634060525517 1201 54-55 2.450550340353086 1201 56-57 2.2582656696660273 1201 58-59 2.1420526452352533 1201 60-61 2.063841349594842 1201 62-63 2.150451984137547 1201 64-65 2.193052685143787 1201 66-67 1.9890831739267192 1201 68-69 1.9303032400892803 1201 70-71 2.1125474596488374 1201 72-73 2.1223431991931214 1201 74-75 2.107065743888988 1201 76-77 2.016523281700323 1201 78-79 1.929185329938047 1201 80-81 2.0894421549123017 1201 82-83 1.989188270263842 1201 84-85 1.8186393737112212 1201 86-87 1.7601155358785014 1201 88-89 1.823551957254459 1201 90-91 1.6784693753667774 1201 92-93 1.6970591800370656 1201 94-95 1.6187989164628753 1201 96-97 1.4514492902336187 1201 98-99 1.451288928038963 1201 100 1.4264546363441113 1202 1 2.9857836819349153 1202 2 7.977247599655193 1202 3 6.694589255308308 1202 4 5.771030318411725 1202 5 5.211773469753716 1202 6 4.671268622609762 1202 7 8.293507411335781 1202 8 6.943768403999265 1202 9 6.7580691031982525 1202 10-11 5.319545729907681 1202 12-13 5.683377500883523 1202 14-15 6.5103927490519915 1202 16-17 5.432019103395845 1202 18-19 4.7390403197797255 1202 20-21 4.852022322686437 1202 22-23 4.856659095662337 1202 24-25 4.395240118733611 1202 26-27 4.398086239615839 1202 28-29 4.181629401033188 1202 30-31 4.070592056550801 1202 32-33 3.9801165730072583 1202 34-35 4.077225460727359 1202 36-37 4.132467768318445 1202 38-39 3.8785700312051326 1202 40-41 3.8742085571998466 1202 42-43 4.030587209492026 1202 44-45 4.409712657507367 1202 46-47 4.206263588218604 1202 48-49 4.050254712747041 1202 50-51 4.043205896499696 1202 52-53 4.418469375802513 1202 54-55 4.393061716142789 1202 56-57 4.187565108631251 1202 58-59 4.0671436479254055 1202 60-61 3.9573026545895296 1202 62-63 4.032975975463575 1202 64-65 4.143519354112723 1202 66-67 3.8859628334670226 1202 68-69 3.8960650290120498 1202 70-71 4.043099282326601 1202 72-73 4.0177395379451255 1202 74-75 3.9361868324750873 1202 76-77 3.9267320619421255 1202 78-79 3.743265299346625 1202 80-81 3.8360996259109896 1202 82-83 3.7255264515537654 1202 84-85 3.66478142980403 1202 86-87 3.7522332794201496 1202 88-89 3.7955945725267384 1202 90-91 3.6954232171337082 1202 92-93 3.7149247997627235 1202 94-95 3.7223365288698282 1202 96-97 3.5872020883416837 1202 98-99 3.453482875023802 1202 100 3.2940657510802858 1203 1 1.5834916136348767 1203 2 6.430947916915368 1203 3 4.927779815418717 1203 4 3.9202268399400317 1203 5 3.5511938572611577 1203 6 2.80790854436367 1203 7 6.339081519287728 1203 8 5.074880178682346 1203 9 4.846578373957925 1203 10-11 3.121029328399299 1203 12-13 3.2876011849567632 1203 14-15 4.184732516718444 1203 16-17 2.766650680907631 1203 18-19 2.1395119125458777 1203 20-21 2.215822962816773 1203 22-23 2.0578420689116044 1203 24-25 1.8549258291571604 1203 26-27 1.8769651673191134 1203 28-29 1.6288919066496312 1203 30-31 1.5963584627336331 1203 32-33 1.452760479039739 1203 34-35 1.4693291892831972 1203 36-37 1.4764431951974792 1203 38-39 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-1.903237583043456 2306 66-67 -2.2883611476477483 2306 68-69 -2.452251898553296 2306 70-71 -2.296786566703254 2306 72-73 -2.3651824132525157 2306 74-75 -1.965277487262945 2306 76-77 -2.364775382273841 2306 78-79 -2.3203574500767274 2306 80-81 -2.294158275943282 2306 82-83 -2.280114557280033 2306 84-85 -2.2385044194993107 2306 86-87 -2.1812032672430455 2306 88-89 -2.200360470579618 2306 90-91 -2.351672695687629 2306 92-93 -2.61216127882523 2306 94-95 -1.9713335958654206 2306 96-97 -1.8357227986589422 2306 98-99 -1.718636558799659 2306 100 -1.6991324433906314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7035382.0 3 1167786.0 4 1020316.0 5 1055310.0 6 1164694.0 7 1292555.0 8 1437961.0 9 1572234.0 10 1527540.0 11 1352795.0 12 1168476.0 13 1026018.0 14 949222.0 15 911454.0 16 905069.0 17 921109.0 18 967626.0 19 1062226.0 20 1178745.0 21 1306723.0 22 1441473.0 23 1587021.0 24 1738883.0 25 1902839.0 26 2087750.0 27 2296664.0 28 2535073.0 29 2818886.0 30 3160192.0 31 3546698.0 32 3889856.0 33 4323998.0 34 4983940.0 35 5768380.0 36 6960999.0 37 8732363.0 38 8769034.0 39 2365164.0 40 5119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.52256752031871 15.658057807792677 14.34657353570218 42.47280113618643 2 18.989426895946643 18.753274718862176 50.875356751725455 11.381941633465727 3 19.220849943512924 25.36915395101072 36.46260711257479 18.94738899290157 4 19.241766429869603 33.9274239848208 31.755321168971406 15.075488416338192 5 17.281810890731556 35.43250182932674 34.01928140739166 13.266405872550044 6 15.004686870060132 35.52676101493103 34.32863211084854 15.139920004160295 7 12.737983749752049 15.887494470001304 52.34546791712298 19.029053863123664 8 14.456123518020558 21.93426056094194 44.39915989551263 19.21045602552487 9 18.91131461711686 19.967058039090645 40.806861420494684 20.314765923297806 10-11 19.525165781824004 33.77798773388556 29.943300107752048 16.753546376538388 12-13 17.347303885982544 28.550222657296736 37.224504149911084 16.877969306809632 14-15 17.47778574690057 27.606722050955206 38.04556153083682 16.869930671307397 16-17 18.136404981193586 27.7938790227616 36.02503913628957 18.044676859755242 18-19 20.970651980223355 27.912098150013488 32.265401684296926 18.85184818546623 20-21 18.77639529563621 30.4132827288905 32.9537946518551 17.856527323618195 22-23 18.3964329203364 30.638981429002456 34.79582703006628 16.16875862059487 24-25 18.163036774173975 29.39710154216276 35.53960419345546 16.900257490207814 26-27 19.1333298453844 29.880703971642973 32.963895845484956 18.022070337487673 28-29 19.41481281921791 30.640392308806042 30.36067716457928 19.584117707396764 30-31 18.45893044487218 29.779270733028234 32.20278099039056 19.559017831709028 32-33 19.294286754798065 29.838015123524368 32.76896922619581 18.09872889548176 34-35 16.718759279627882 31.400409194419943 31.500883313965016 20.37994821198716 36-37 17.88694725137571 29.572464975378367 32.11526708185495 20.42532069139097 38-39 19.063352830750894 28.508023748692402 32.685568240726575 19.743055179830137 40-41 20.79662831866021 29.100738342290033 31.48239308451668 18.620240254533073 42-43 20.1647884576034 30.80921920328816 30.52156466098415 18.50442767812429 44-45 19.081736598675906 30.388984004522644 31.22829496200209 19.300984434799357 46-47 18.951101070340968 29.638679233474 31.351987395067297 20.05823230111773 48-49 20.593785729944237 29.86709298863764 30.36227378241099 19.176847499007135 50-51 19.690313343801318 30.184489114362652 31.357214296114222 18.767983245721812 52-53 18.484559366562863 30.885224602047177 32.98152406884785 17.648691962542117 54-55 19.219969098911857 30.751510781115222 31.991522927710886 18.036997192262035 56-57 20.428686136412672 28.673902242606125 31.622554838288103 19.2748567826931 58-59 19.1934480489953 29.516930785767492 30.515099487216286 20.774521678020918 60-61 20.584327049693584 29.730039108652097 30.971633046332297 18.714000795322026 62-63 17.131591975737074 34.61323886675481 30.35387323940066 17.90129591810745 64-65 16.708681089647904 32.56476703359507 32.06602298762855 18.66052888912848 66-67 17.964113857657015 28.79003737457853 33.53098035706995 19.714868410694507 68-69 20.475305535988976 31.155802121834213 29.11377315346663 19.255119188710175 70-71 18.779991912285855 33.075685050956274 29.271906602245984 18.872416434511887 72-73 17.847832807528725 31.924677395442064 32.0149284153763 18.21256138165291 74-75 18.65538561850388 30.10136322997016 32.12827776051538 19.114973391010576 76-77 19.65293901115441 30.23130041302235 30.934535560034504 19.18122501578874 78-79 19.18259271564196 32.25883486452628 29.460777943140876 19.097794476690886 80-81 18.60739600348134 31.990961151336748 32.083884338428604 17.31775850675331 82-83 17.74862140518561 31.979073388906503 32.1374160504946 18.13488915541329 84-85 18.579950078326938 31.370291721781406 31.096625580256347 18.95313261963531 86-87 19.838195166227763 31.74610035793666 29.263823923744415 19.151880552091164 88-89 18.465935709811145 31.7103613147805 30.608263769513066 19.215439205895287 90-91 18.483434396666045 31.722243762366325 30.501602967712632 19.292718873254998 92-93 17.634200160454398 32.15620865109945 30.74634122195054 19.46324996649561 94-95 17.41909209854536 32.00659224791191 30.23252362876658 20.341792024776158 96-97 18.46548901219987 30.041228143649786 31.541200306808864 19.952082537341482 98-99 19.757906077853594 30.93331977042541 30.068658339239395 19.2401158124816 100 20.425293640954933 31.448954651769384 29.597970764319037 18.52778094295665 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6096.0 1 7848.5 2 8901.0 3 8930.0 4 11015.5 5 14690.5 6 18650.0 7 22816.5 8 28872.0 9 35991.0 10 44274.5 11 54137.5 12 65812.5 13 79336.0 14 95885.5 15 116252.0 16 140229.5 17 169748.5 18 207133.5 19 253709.0 20 312245.0 21 386412.0 22 478020.0 23 591520.5 24 733155.0 25 908500.5 26 1117253.5 27 1367916.5 28 1671676.0 29 2050650.0 30 2532028.5 31 3138087.0 32 3920939.5 33 4870956.0 34 5881418.5 35 6788644.5 36 7330056.0 37 7150340.5 38 6255096.5 39 5128185.5 40 4183791.5 41 3523781.5 42 3070109.0 43 2748723.0 44 2492791.5 45 2273564.0 46 2088684.5 47 1912991.0 48 1745481.5 49 1586532.5 50 1420720.5 51 1252101.5 52 1093548.0 53 945483.0 54 799518.5 55 661474.0 56 537239.5 57 422297.5 58 323116.5 59 241338.0 60 175702.0 61 126076.5 62 90685.5 63 64435.5 64 44257.5 65 30170.5 66 20634.5 67 14393.0 68 10347.5 69 7563.5 70 5639.0 71 4309.5 72 3482.5 73 2922.0 74 2493.5 75 1982.0 76 1582.5 77 1349.5 78 1122.5 79 996.0 80 869.5 81 671.0 82 538.5 83 435.5 84 314.0 85 204.0 86 156.0 87 122.5 88 93.0 89 77.0 90 68.5 91 61.0 92 51.5 93 31.5 94 23.0 95 20.0 96 9.0 97 6.5 98 4.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.04685842072071767 2 20.490902914247222 3 8.935078470854082 4 3.1011438276094507 5 2.7879136845672092 6 0.021933359528931762 7 17.943705833919328 8 5.972686294768607 9 6.153129810557997 10-11 0.008280785148719175 12-13 4.308644650608199 14-15 4.59888412795363 16-17 1.5425790671778234 18-19 0.03689033625532052 20-21 1.5816876532155844 22-23 0.08092859315596886 24-25 0.08036343722750817 26-27 0.07843057332041505 28-29 0.07363619272043835 30-31 0.07308226843644573 32-33 0.07104372496549409 34-35 0.03395683493198264 36-37 0.019100432476512358 38-39 0.01622155778763274 40-41 0.01825703808282037 42-43 0.05639510793268279 44-45 0.10502200212782484 46-47 0.15816299634053624 48-49 0.10156367669025247 50-51 0.08150191755313356 52-53 0.08004231430158067 54-55 0.07129235273167327 56-57 0.05716345452015643 58-59 0.02477547610864321 60-61 0.021708216110276696 62-63 0.01802270513687326 64-65 0.029340118526318803 66-67 0.030065580653096235 68-69 0.06438335979593858 70-71 0.08598691740094479 72-73 0.1008698673797032 74-75 0.10177758846443949 76-77 0.06293243554238372 78-79 0.03111063411791918 80-81 0.02111293895346988 82-83 0.025445290542374373 84-85 0.03226494085165864 86-87 0.03308382983923851 88-89 0.04475657161730973 90-91 0.03584119855614556 92-93 0.02936309234454891 94-95 0.024590153974920332 96-97 0.028340502168662074 98-99 0.05407475229144181 100 0.06844053609537577 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9.7937573E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.22095050783884 #Duplication Level Percentage of deduplicated Percentage of total 1 89.92339603609072 62.24582946511026 2 5.6073766044493505 7.762958768308037 3 1.7672751325417981 3.669973934502304 4 0.848509625570119 2.3493857118805623 5 0.47200547459096653 1.6336333798045135 6 0.2946477851791689 1.2237479853078947 7 0.19923556354797425 0.9653892558628993 8 0.1435134977055781 0.794731257750773 9 0.10567342438089812 0.6583333391157598 >10 0.5625702788549677 6.9928152249520235 >50 0.04293439775414655 2.0670038979795877 >100 0.028572508560219777 3.770127052061314 >500 0.0026009360327869774 1.234663262772383 >1k 0.001445334838708136 1.8287067229169012 >5k 1.3763735397555066E-4 0.6634630237429104 >10k+ 1.0576254867324594E-4 2.139237717932014 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 202027 0.20628140335885187 No Hit GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG 193542 0.1976177212396309 No Hit AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA 159457 0.16281493926748625 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0026527102116365494 3.063175764014491E-6 0.0 2.0421171760096608E-6 2.0421171760096608E-6 2 0.002672110324808641 9.189527292043473E-6 0.0 5.105292940024152E-6 2.0421171760096608E-6 3 0.0027201000784448683 1.0210585880048303E-5 0.0 6.126351528028982E-6 2.0421171760096608E-6 4 0.0030856390529505973 1.0210585880048303E-5 0.0 1.2252703056057964E-5 2.0421171760096608E-6 5 0.0031397551581148534 1.0210585880048303E-5 0.0 1.2252703056057964E-5 5.105292940024152E-6 6 0.0032285872552712735 1.0210585880048303E-5 0.0 1.7357995996082118E-5 5.105292940024152E-6 7 0.003291892887727573 1.4294820232067626E-5 0.0 2.34843475241111E-5 5.105292940024152E-6 8 0.003675810916817389 2.144223034810144E-5 0.0 3.675810916817389E-5 5.105292940024152E-6 9 0.0037707693655018386 2.552646470012076E-5 0.0 4.390551928420771E-5 5.105292940024152E-6 10-11 0.004220545673517966 2.654752328812559E-5 0.0 5.0542400106239107E-5 5.105292940024152E-6 12-13 0.004896486458777164 2.7058052582128007E-5 0.0 5.6668751634268084E-5 5.105292940024152E-6 14-15 0.005739370323175152 2.8589640464135252E-5 0.0 6.636880822031397E-5 5.105292940024152E-6 16-17 0.006803313371876184 3.165281622814974E-5 0.0 8.423733351039851E-5 6.636880822031397E-6 18-19 0.00793107258232752 3.420546269816182E-5 0.0 9.036368503842749E-5 8.168468704038643E-6 20-21 0.009132348011115204 4.645816575421978E-5 0.0 1.0108480021247821E-4 8.168468704038643E-6 22-23 0.01027082833674059 5.105292940024152E-5 0.0 1.1078485679852409E-4 1.0210585880048303E-5 24-25 0.011373061082491802 5.207398798824635E-5 5.105292940024152E-7 1.2303755985458207E-4 1.0210585880048303E-5 26-27 0.012416072430138737 5.462663445825842E-5 1.0210585880048304E-6 1.3018496997061588E-4 1.174217376205555E-5 28-29 0.013583142396228258 5.7179280928270504E-5 1.0210585880048304E-6 1.3631132149864486E-4 1.480534952607004E-5 30-31 0.014675675085393428 6.0752985986287405E-5 1.0210585880048304E-6 1.449903194966859E-4 1.5315878820072454E-5 32-33 0.015738597075506455 6.330563245629948E-5 1.0210585880048304E-6 1.5111667102471487E-4 1.6336937408077286E-5 34-35 0.01691689868606403 6.432669104430432E-5 1.0210585880048304E-6 1.7051678419680667E-4 1.7357995996082118E-5 36-37 0.018075800183449512 6.534774963230914E-5 1.0210585880048304E-6 1.888958387808936E-4 2.0931701054099023E-5 38-39 0.019300559959761307 6.738986680831881E-5 1.0210585880048304E-6 2.0370118830696367E-4 2.2463288936106267E-5 40-41 0.020832658371062553 6.994251327833089E-5 1.0210585880048304E-6 2.1391177418701198E-4 2.2463288936106267E-5 42-43 0.022457673113872242 7.147410116033812E-5 1.0210585880048304E-6 2.2973818230108683E-4 2.2463288936106267E-5 44-45 0.02406635091927385 7.351621833634779E-5 1.0210585880048304E-6 2.4505406112115927E-4 2.2463288936106267E-5 46-47 0.025934888135322694 7.555833551235745E-5 1.0210585880048304E-6 2.573067641772173E-4 2.2463288936106267E-5 48-49 0.027951989375926235 7.606886480635987E-5 1.0210585880048304E-6 2.664962914692607E-4 2.2463288936106267E-5 50-51 0.03042601433466194 7.760045268836711E-5 1.0210585880048304E-6 2.7772793593731384E-4 2.2973818230108683E-5 52-53 0.03297763974608601 8.015309915837918E-5 1.0210585880048304E-6 2.8640693393535495E-4 2.34843475241111E-5 54-55 0.03570080300029489 8.372680421639609E-5 1.0210585880048304E-6 2.9712804910940563E-4 2.34843475241111E-5 56-57 0.039248981593611676 8.474786280440092E-5 1.0210585880048304E-6 3.1754922086950224E-4 2.34843475241111E-5 58-59 0.04309888299968389 8.678997998041058E-5 1.0210585880048304E-6 3.318440411015699E-4 2.4505406112115928E-5 60-61 0.04722855445886943 1.041479759764927E-4 1.0210585880048304E-6 3.435862148636254E-4 2.5015935406118344E-5 62-63 0.05237468974241377 1.1027432750452168E-4 1.0210585880048304E-6 3.660495037997317E-4 2.552646470012076E-5 64-65 0.05797111186326825 1.1589014973854825E-4 1.0210585880048304E-6 3.757495603857776E-4 2.756858187613042E-5 66-67 0.064893378560647 1.3529026291064002E-4 1.0210585880048304E-6 3.8749173414783314E-4 2.8079111170132836E-5 68-69 0.0734743549342396 1.5264825890672214E-4 1.0210585880048304E-6 3.961707321458742E-4 2.8589640464135252E-5 70-71 0.08427051791450867 1.541798467887294E-4 1.0210585880048304E-6 4.1046555237794183E-4 2.961069905214008E-5 72-73 0.09664881117689123 1.6030619831675837E-4 1.0210585880048304E-6 4.268024897860191E-4 3.063175764014491E-5 74-75 0.11191772130191546 1.6234831549276802E-4 1.0210585880048304E-6 4.405867807240843E-4 3.063175764014491E-5 76-77 0.13115905986357249 1.6847466702079702E-4 1.0210585880048304E-6 4.661132454242051E-4 3.063175764014491E-5 78-79 0.15601264695419806 1.7000625490280427E-4 1.0210585880048304E-6 4.875554757723065E-4 3.063175764014491E-5 80-81 0.18641517694133589 1.7357995996082116E-4 1.0210585880048304E-6 5.110398232964176E-4 3.063175764014491E-5 82-83 0.2241289969478823 1.7562207713683084E-4 1.0210585880048304E-6 5.365662879965383E-4 3.063175764014491E-5 84-85 0.27650317616100206 1.7919578219484773E-4 1.0210585880048304E-6 5.488189910525963E-4 3.063175764014491E-5 86-87 0.34855060171850494 1.8021684078285254E-4 1.0210585880048304E-6 5.615822234026568E-4 3.063175764014491E-5 88 0.4139575727489183 1.8276948725286463E-4 1.0210585880048304E-6 5.789402193987389E-4 3.063175764014491E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 98890 0.0 80.967834 1 GCGTGTT 115685 0.0 65.11336 2 CGTGTTT 172610 0.0 44.211746 3 CGCGAGT 4880 0.0 42.460957 1 CGCGTAT 3805 0.0 39.505043 1 CGGCGAG 12740 0.0 39.344997 1 CCGCGTG 5660 0.0 38.302372 1 CTGGAAT 326880 0.0 35.679817 1 CGCGTGC 5430 0.0 35.51318 1 GTTTTTT 1292965 0.0 32.46452 1 CGCGCGT 3610 0.0 32.349007 1 CGCGGGT 5865 0.0 31.653898 1 CGCGTGG 5565 0.0 28.410065 1 CTCGTGT 14225 0.0 26.986046 1 CGCGTGA 4385 0.0 24.309942 1 GCGCGTT 4365 0.0 23.598246 2 CGCGTTT 11405 0.0 23.261726 1 CTGGCGA 13605 0.0 22.361383 1 GGAAAAC 615220 0.0 20.601593 1 GTGTTTT 349630 0.0 20.329361 4 >>END_MODULE