FastQCFastQC Report
Fri 27 May 2016
SRR1233069_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233069_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97937573
Sequences flagged as poor quality0
Sequence length100
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC2706920.2763923913042035No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG2219700.2266443747794322No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA1920670.19611165982232376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT1202600.066.8954851
GCGTGTT1371300.057.067832
CGTGTTT1964000.040.9752583
CGGGAAT150800.039.616261
GGTAAAC467550.037.4371451
CGCTTGT152450.036.317851
CCGCGTG53400.036.0290761
GTAAACT919150.034.6642272
GCGGGTT83800.034.2979621
TAACTGA1201200.033.81294
CGCGTTT127800.033.7527851
CGGCGAG139600.031.7421671
TAAACTG1157500.031.2192863
CCGGAAT93700.030.2223341
CTGGAAT3622850.029.8848171
GATAACT937100.029.071362
GGATAAC508800.028.993461
CGGGTTT204850.027.946391
ATAACTG1020650.027.8338473
GCGCGTT53750.026.1675971
Produced by FastQC (version 0.11.5)