##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233069_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 97937573 Sequences flagged as poor quality 0 Sequence length 100 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.169186845175343 28.0 18.0 31.0 2.0 33.0 2 24.250015885118984 30.0 16.0 31.0 2.0 33.0 3 22.296237267386644 27.0 16.0 31.0 2.0 33.0 4 23.31418093237822 27.0 16.0 35.0 2.0 37.0 5 26.290530468832426 32.0 19.0 35.0 2.0 37.0 6 27.76251152353959 33.0 26.0 35.0 2.0 37.0 7 28.74028720315542 35.0 28.0 35.0 2.0 37.0 8 28.899791053633727 35.0 28.0 37.0 2.0 37.0 9 30.02841745935444 35.0 29.0 39.0 2.0 39.0 10-11 29.904489531305828 35.0 28.5 39.0 2.0 39.0 12-13 29.71938859971545 35.0 27.5 39.0 2.0 39.0 14-15 30.47643584143136 36.0 27.5 40.0 2.0 41.0 16-17 30.250077654058263 36.0 27.0 40.0 2.0 41.0 18-19 30.01838447640519 36.0 27.0 40.0 2.0 41.0 20-21 29.765904868808622 36.0 27.0 40.0 2.0 41.0 22-23 29.586377196625037 36.0 27.0 40.0 2.0 41.0 24-25 29.39569625132532 36.0 26.0 40.0 2.0 41.0 26-27 29.094737813239462 36.0 24.5 40.0 2.0 41.0 28-29 28.843656627063854 36.0 24.0 40.0 2.0 41.0 30-31 28.563275684807913 36.0 23.0 40.0 2.0 41.0 32-33 28.34174859019633 35.5 22.0 40.0 2.0 40.0 34-35 28.117510768824136 35.0 20.0 40.0 2.0 40.0 36-37 27.89134782316895 35.0 18.5 39.0 2.0 40.0 38-39 27.688891877073573 35.0 18.0 39.0 2.0 40.0 40-41 27.46624328234068 35.0 17.0 39.0 2.0 40.0 42-43 27.336427588419003 34.5 16.0 39.0 2.0 40.0 44-45 27.027645748378916 34.0 15.0 38.0 2.0 40.0 46-47 26.70605046543271 33.5 10.5 38.0 2.0 40.0 48-49 26.437546006985492 33.0 9.0 38.0 2.0 40.0 50-51 26.10723657609935 33.0 8.5 38.0 2.0 40.0 52-53 25.839183282599826 33.0 7.5 38.0 2.0 40.0 54-55 25.600435876637455 33.0 2.0 38.0 2.0 40.0 56-57 25.23931864740001 32.5 2.0 37.5 2.0 39.5 58-59 24.8441857089924 32.0 2.0 37.0 2.0 39.0 60-61 24.37481372445282 31.5 2.0 37.0 2.0 39.0 62-63 23.93535723516449 31.0 2.0 36.0 2.0 39.0 64-65 23.53655361155417 31.0 2.0 36.0 2.0 38.0 66-67 23.18824270844449 30.5 2.0 35.5 2.0 38.0 68-69 22.83317507265572 30.0 2.0 35.0 2.0 38.0 70-71 22.329310559901252 29.0 2.0 34.5 2.0 38.0 72-73 21.88051792441293 29.0 2.0 34.0 2.0 37.0 74-75 21.439291123744717 29.0 2.0 34.0 2.0 36.5 76-77 19.88946022278906 26.0 2.0 31.5 2.0 34.5 78-79 20.332013128403744 28.0 2.0 32.5 2.0 35.0 80-81 20.10418317697131 27.5 2.0 33.0 2.0 35.0 82-83 19.7458091237364 26.0 2.0 32.0 2.0 35.0 84-85 19.169347227953054 26.0 2.0 32.0 2.0 34.0 86-87 18.605192799703133 25.5 2.0 32.0 2.0 34.0 88-89 17.94323326758363 25.0 2.0 31.0 2.0 34.0 90-91 17.396711454142324 23.5 2.0 31.0 2.0 34.0 92-93 16.76344071238114 20.5 2.0 31.0 2.0 34.0 94-95 16.024641809328887 17.5 2.0 30.5 2.0 34.0 96-97 15.18765925004084 4.5 2.0 30.0 2.0 33.0 98-99 14.335823371894257 2.0 2.0 29.0 2.0 32.5 100 13.677766499278066 2.0 2.0 29.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -3.719167725365825 1101 2 -4.77942675835623 1101 3 -3.107470608677726 1101 4 -3.875749473842866 1101 5 -2.6225957825711106 1101 6 -1.6908411091737712 1101 7 -1.6511165280276892 1101 8 -1.5413256817703918 1101 9 -1.6555794352041282 1101 10-11 -1.702181713548427 1101 12-13 -1.5730892774776173 1101 14-15 -1.790828563292365 1101 16-17 -1.7791524821139966 1101 18-19 -1.847833342436715 1101 20-21 -1.8402294978853142 1101 22-23 -1.837603775525853 1101 24-25 -1.9570079911801201 1101 26-27 -2.0186476246464373 1101 28-29 -1.8640767271201426 1101 30-31 -2.044006395861608 1101 32-33 -1.8946738375405694 1101 34-35 -1.8835871255908856 1101 36-37 -1.8558678239394801 1101 38-39 -1.9628502750583294 1101 40-41 -1.859962601887485 1101 42-43 -1.8650988669784638 1101 44-45 -1.8662059499661012 1101 46-47 -1.7649961659424882 1101 48-49 -1.7627861649062773 1101 50-51 -1.7433512237369548 1101 52-53 -1.641939723882679 1101 54-55 -1.8670954038099516 1101 56-57 -1.780396604015408 1101 58-59 -1.699503057513354 1101 60-61 -1.6118031917409255 1101 62-63 -1.6496594565618814 1101 64-65 -1.6484182693383325 1101 66-67 -1.712325988781302 1101 68-69 -1.633934650186582 1101 70-71 -1.570387404564073 1101 72-73 -1.6007173025839165 1101 74-75 -1.6181936927513618 1101 76-77 -1.5109531515960946 1101 78-79 -1.4886511607348254 1101 80-81 -1.551221436388012 1101 82-83 -1.6409841218675396 1101 84-85 -1.4724426101464445 1101 86-87 -1.4312857953558833 1101 88-89 -1.5210995398322495 1101 90-91 -1.4924359291179705 1101 92-93 -1.459584190669812 1101 94-95 -1.4801336020150409 1101 96-97 -1.3793066251641548 1101 98-99 -1.3013177105388554 1101 100 -1.1489473269284254 1102 1 -2.5471438951111303 1102 2 -4.301016570882375 1102 3 -3.2118474873435083 1102 4 -1.8027575402937153 1102 5 -1.0912705051271345 1102 6 -0.6037710882554528 1102 7 -0.5138613787387598 1102 8 -0.4753906075374985 1102 9 -0.46453819668869656 1102 10-11 -0.3824018506096607 1102 12-13 -0.30289008981079846 1102 14-15 -0.3267117463059783 1102 16-17 -0.4127803582717924 1102 18-19 -0.468537441696391 1102 20-21 -0.6595845381452605 1102 22-23 -0.43466959150674356 1102 24-25 -0.42043013079241476 1102 26-27 -0.440774323248192 1102 28-29 -0.37492406038943216 1102 30-31 -0.32623711414468204 1102 32-33 -0.3846130360906379 1102 34-35 -0.42982277388207635 1102 36-37 -0.43459205167516757 1102 38-39 -0.2685714351050308 1102 40-41 -0.5384323173722052 1102 42-43 -0.4791548670366126 1102 44-45 -0.2721933977794855 1102 46-47 -0.28095116807523013 1102 48-49 -0.359883581913234 1102 50-51 -0.3278755085695444 1102 52-53 -0.17178908498495815 1102 54-55 -0.15638467452140503 1102 56-57 -0.19063143725524156 1102 58-59 -0.19973527700940252 1102 60-61 -0.16291472942648966 1102 62-63 -0.19265339984701413 1102 64-65 -0.14738708756150132 1102 66-67 -0.11373946535562851 1102 68-69 -0.03309292716592438 1102 70-71 -0.0355039698470172 1102 72-73 -0.029156716286955486 1102 74-75 -0.05880416503248753 1102 76-77 -0.056741125519536695 1102 78-79 -0.09117178205208631 1102 80-81 -0.03939517431311401 1102 82-83 -0.04158081159268079 1102 84-85 0.0875786945028949 1102 86-87 0.08213954111929667 1102 88-89 0.0595535979014592 1102 90-91 0.07149068151499094 1102 92-93 0.1082326826196045 1102 94-95 0.10797067443902719 1102 96-97 0.19287833885887196 1102 98-99 0.20263568577535196 1102 100 0.2778536846733797 1103 1 -0.5143770541288575 1103 2 1.5489772878424652 1103 3 -0.9055311180325454 1103 4 -0.8417618485913074 1103 5 -0.5740301549807505 1103 6 -0.14431368571882786 1103 7 -0.16630075172487935 1103 8 -0.016729380873279354 1103 9 0.03982783530026168 1103 10-11 0.0469975308013133 1103 12-13 -0.048194789348823264 1103 14-15 -0.061382031251781655 1103 16-17 0.021883810674399484 1103 18-19 0.036734076966329354 1103 20-21 0.08325311107088851 1103 22-23 -0.09741045975856011 1103 24-25 -0.04318898911242641 1103 26-27 -0.08661665419445441 1103 28-29 -0.0728072574569083 1103 30-31 -0.03374273671793304 1103 32-33 -0.03972158093518274 1103 34-35 -0.35345120494103455 1103 36-37 -0.05847676139203628 1103 38-39 0.11653153447933562 1103 40-41 -0.0019492746814293582 1103 42-43 0.008234982880978237 1103 44-45 0.020239350218190566 1103 46-47 -0.30041682897307354 1103 48-49 -0.19492463221447665 1103 50-51 0.1201803316908645 1103 52-53 0.06426841500066871 1103 54-55 0.1536316118151504 1103 56-57 0.08936360541785149 1103 58-59 0.05292505328850439 1103 60-61 0.07144763279210764 1103 62-63 0.005164559622567566 1103 64-65 0.18227088577832262 1103 66-67 0.09418208730398092 1103 68-69 0.16709926852536228 1103 70-71 0.07919137126537024 1103 72-73 0.2567600199622575 1103 74-75 0.2557359411176314 1103 76-77 0.25082600524002885 1103 78-79 0.3117563590934722 1103 80-81 0.2861070714865477 1103 82-83 0.342194578526545 1103 84-85 0.44819858789008293 1103 86-87 0.4322818870906744 1103 88-89 0.43829397826071315 1103 90-91 0.3927701795885987 1103 92-93 0.27618987488029134 1103 94-95 0.2099410492304017 1103 96-97 0.3804425530411528 1103 98-99 0.4134144207997288 1103 100 0.4202166806725476 1104 1 3.7333636480091847 1104 2 4.263814273978404 1104 3 6.375833481175988 1104 4 1.8614981696307673 1104 5 2.702441572231276 1104 6 2.409459222537695 1104 7 2.0317805488324865 1104 8 1.9033418596883571 1104 9 2.0165711148722103 1104 10-11 1.9890307744610034 1104 12-13 1.9778583655439554 1104 14-15 2.1069753576421384 1104 16-17 1.9866675632555335 1104 18-19 1.8559477862894553 1104 20-21 2.0265874404007036 1104 22-23 2.010539256952928 1104 24-25 1.9926255879074048 1104 26-27 2.0233602291564843 1104 28-29 2.163321221194714 1104 30-31 2.1674297791178816 1104 32-33 2.1123779521393153 1104 34-35 2.1495348604895383 1104 36-37 1.9990519472824388 1104 38-39 1.9322480232226695 1104 40-41 1.9527193923569754 1104 42-43 2.0543316720473612 1104 44-45 1.957158683236326 1104 46-47 1.8246839042901435 1104 48-49 1.9774007378293632 1104 50-51 2.027972114639997 1104 52-53 2.130688488712984 1104 54-55 2.066637603881368 1104 56-57 1.9903486111564774 1104 58-59 1.8346200302194262 1104 60-61 1.8458513583467706 1104 62-63 1.8647078382005269 1104 64-65 2.012438828888275 1104 66-67 1.6447232007932904 1104 68-69 1.7602343644159149 1104 70-71 1.9061888158596005 1104 72-73 1.8203162993655155 1104 74-75 1.618324057291158 1104 76-77 1.5271967416302985 1104 78-79 1.5984010193949665 1104 80-81 1.713083735298369 1104 82-83 1.7598419876435187 1104 84-85 1.8810332236257423 1104 86-87 1.5972655839879764 1104 88-89 1.3052482171286002 1104 90-91 1.3538606768126051 1104 92-93 1.1253228056362161 1104 94-95 1.009396482419639 1104 96-97 0.9511108250218498 1104 98-99 0.73758415788798 1104 100 0.7033147637548343 1105 1 2.05756955169349 1105 2 2.73597244985487 1105 3 0.5201020004859878 1105 4 0.841484685313695 1105 5 -0.4982147826304235 1105 6 -0.6474224104497495 1105 7 -0.274121500675399 1105 8 -0.14406375559396523 1105 9 -0.3533759491030999 1105 10-11 -0.4789143643353455 1105 12-13 -2.075005648488201 1105 14-15 -1.3673488795921713 1105 16-17 -0.5051613049474035 1105 18-19 -0.28150490381345605 1105 20-21 -0.34964358649899907 1105 22-23 -0.2395943293174838 1105 24-25 -0.11215174642538628 1105 26-27 -0.21980848136632858 1105 28-29 -0.21156826097727333 1105 30-31 -0.07968046622190528 1105 32-33 -0.05127982897183969 1105 34-35 -0.06042022561162241 1105 36-37 -0.011127622305934892 1105 38-39 -0.10663514963207632 1105 40-41 -0.18858390332376374 1105 42-43 -0.28213474601625066 1105 44-45 -0.33833390600710445 1105 46-47 -0.3883537636003851 1105 48-49 -0.535816840996997 1105 50-51 -0.5364645998073172 1105 52-53 -0.5052928126977427 1105 54-55 -0.35438064014835646 1105 56-57 -0.44566502070772884 1105 58-59 -0.3659497579638753 1105 60-61 -0.14142205682974662 1105 62-63 -0.3087467133989641 1105 64-65 -0.6691044652712854 1105 66-67 -0.5773560739355759 1105 68-69 -0.607615704666749 1105 70-71 -0.8159122265182752 1105 72-73 -0.6553441576402257 1105 74-75 -0.6247995928143091 1105 76-77 -0.9597667090800357 1105 78-79 -0.9219451390757172 1105 80-81 -0.9577662515589616 1105 82-83 -0.9053680634992816 1105 84-85 -1.2475299506273814 1105 86-87 -1.2051196159337039 1105 88-89 -1.0872389374118185 1105 90-91 -1.4170047211366885 1105 92-93 -1.4011143048755113 1105 94-95 -1.3258953797361475 1105 96-97 -1.4039452480000527 1105 98-99 -1.4348280974105059 1105 100 -1.4272068635928292 1201 1 2.410395651731225 1201 2 0.5139927727386784 1201 3 -0.3613683098614082 1201 4 0.08071268565984013 1201 5 0.7852384423840313 1201 6 1.300842477379895 1201 7 1.2357874409993208 1201 8 1.3391536669481816 1201 9 1.3847281578894624 1201 10-11 1.6063682111437387 1201 12-13 1.6422524972599675 1201 14-15 1.659260158843022 1201 16-17 1.6648393534894232 1201 18-19 1.4650510326671977 1201 20-21 1.641372979587885 1201 22-23 1.545598030113485 1201 24-25 1.6097322234373799 1201 26-27 1.5407811912799652 1201 28-29 1.6672699707964185 1201 30-31 1.612382344138389 1201 32-33 1.5544807933588167 1201 34-35 1.5349059386952746 1201 36-37 1.5129369889559285 1201 38-39 1.7064809309704216 1201 40-41 1.6701824264978384 1201 42-43 1.5489198253073297 1201 44-45 1.7455203281167684 1201 46-47 1.8526119328348472 1201 48-49 1.7000412278625952 1201 50-51 1.574892156544621 1201 52-53 1.7043803905193542 1201 54-55 1.6793557109015929 1201 56-57 1.652605782778295 1201 58-59 1.7125259413272111 1201 60-61 1.7217709575421196 1201 62-63 1.8254771850274771 1201 64-65 1.6983391145126276 1201 66-67 1.7642611832632298 1201 68-69 1.8588898977669963 1201 70-71 1.7733569337570039 1201 72-73 1.8734524830212962 1201 74-75 1.8584928899117301 1201 76-77 1.7263961494950735 1201 78-79 1.6947274948443685 1201 80-81 1.7062270130151234 1201 82-83 1.604362709830827 1201 84-85 1.5718490347321001 1201 86-87 1.7299297659107538 1201 88-89 1.692720722281635 1201 90-91 1.7916747962498718 1201 92-93 1.7864442740634026 1201 94-95 1.8005935355299556 1201 96-97 1.9096254667303825 1201 98-99 1.7858247460792 1201 100 1.80701270013558 1202 1 5.370110573525281 1202 2 4.07060518932974 1202 3 7.315327462037509 1202 4 10.080017703617944 1202 5 6.516031920498801 1202 6 4.963294922216981 1202 7 3.873328260250183 1202 8 3.7067184044263364 1202 9 3.8584386072045156 1202 10-11 3.7440058554816957 1202 12-13 3.910286612084935 1202 14-15 4.144323525043813 1202 16-17 3.992894509631892 1202 18-19 3.965943577374688 1202 20-21 3.87237147110865 1202 22-23 3.9168976497327783 1202 24-25 3.8939840059213147 1202 26-27 4.048540225484356 1202 28-29 4.080017798314451 1202 30-31 4.12638805797809 1202 32-33 4.034965424123424 1202 34-35 4.064724912995931 1202 36-37 4.180967238288176 1202 38-39 4.141333861945654 1202 40-41 4.152142845580045 1202 42-43 4.178149712792074 1202 44-45 4.1613391202956045 1202 46-47 4.223372665373592 1202 48-49 4.094691266195515 1202 50-51 4.230351751996874 1202 52-53 4.233765079848464 1202 54-55 4.195908145043493 1202 56-57 4.1634138924370205 1202 58-59 3.996119953326705 1202 60-61 4.042848554897198 1202 62-63 4.029515138287305 1202 64-65 4.04684801739587 1202 66-67 4.045395072100433 1202 68-69 4.085451629693878 1202 70-71 4.151814357498846 1202 72-73 4.04283319829679 1202 74-75 3.9557263600679597 1202 76-77 3.6406810613036313 1202 78-79 3.9217576763200412 1202 80-81 3.967104935127921 1202 82-83 3.9143830637784305 1202 84-85 3.8980301363449428 1202 86-87 3.9486990899649186 1202 88-89 4.017259033662846 1202 90-91 3.923498382467315 1202 92-93 3.8741382720079756 1202 94-95 3.9258321439544943 1202 96-97 3.8341933385593236 1202 98-99 3.6448954952272423 1202 100 3.6017358857327846 1203 1 1.8590154209442034 1203 2 -0.36422207666645434 1203 3 0.8455218564127271 1203 4 -0.27943465544099055 1203 5 0.32867376765832645 1203 6 0.8028189018281751 1203 7 0.6244609020746843 1203 8 0.6555007569233808 1203 9 0.774438563491497 1203 10-11 0.8350019982300765 1203 12-13 0.7694389735807796 1203 14-15 0.8369348020265512 1203 16-17 0.8333738930669234 1203 18-19 0.8460198635332574 1203 20-21 0.931285666981438 1203 22-23 0.8543269416174226 1203 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2306 80-81 -2.0982421876118806 2306 82-83 -1.8769027862402439 2306 84-85 -1.9300630105135426 2306 86-87 -1.9129511521343616 2306 88-89 -1.7715517392380065 2306 90-91 -1.7677400543026298 2306 92-93 -1.9021712321243385 2306 94-95 -1.8642498309952913 2306 96-97 -1.836357702865282 2306 98-99 -1.8488376941825226 2306 100 -1.869343652080044 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 1.1043918E7 3 1842657.0 4 1232183.0 5 1144103.0 6 1188244.0 7 1083829.0 8 1006366.0 9 1097402.0 10 1149080.0 11 1105428.0 12 1044887.0 13 1009200.0 14 1009685.0 15 1038674.0 16 1077697.0 17 1117023.0 18 1158093.0 19 1198138.0 20 1238573.0 21 1277064.0 22 1336354.0 23 1424148.0 24 1546121.0 25 1714640.0 26 1952980.0 27 2307406.0 28 2690910.0 29 3042975.0 30 3679550.0 31 4185480.0 32 4910248.0 33 6161721.0 34 7979422.0 35 1.0283054E7 36 9333530.0 37 2281726.0 38 44901.0 39 163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.71627196431999 16.18550677728325 17.245291784096896 38.85292947429987 2 20.848112228884304 18.68396136027929 50.085759646752805 10.382166764083602 3 21.986924566660825 24.050427421312254 37.80428426932564 16.158363742701273 4 22.36610048342161 31.17882986025446 33.445246646437965 13.00982300988596 5 17.275318407048964 36.52343421308678 33.48519493613886 12.71605244372539 6 14.979619830915375 36.5448413654988 33.7327411592412 14.74279764434463 7 12.724973109831724 15.993915514836749 52.584637963615954 18.696473411715576 8 14.43566419495296 22.094402163450138 44.61576865761209 18.854164983984816 9 19.02863109242903 20.057090718078 41.059787235376525 19.85449095411644 10-11 19.780836822663375 34.264596423820095 29.65004319654494 16.304523556971596 12-13 17.476333998614464 28.939021637213465 37.04381897528563 16.54082538888644 14-15 17.680492668293496 27.949326842356946 37.85936094163135 16.510819547718203 16-17 18.388171180203294 28.17028327484689 35.724193420066946 17.71735212488287 18-19 21.336288467840397 28.174249531226252 31.92699125102887 18.56247074990448 20-21 18.926367599930312 30.56020736136099 32.68308277441201 17.830342264296686 22-23 18.661386648733668 31.200202085258272 34.37450656396178 15.763904702046277 24-25 18.382426369176507 29.770036315488696 35.19817650208089 16.649360813253907 26-27 19.264624644323085 30.125851883604483 32.7336480519637 17.87587542010873 28-29 19.43228000435081 30.906349688644568 30.048118288474544 19.613252018530076 30-31 18.76476905076595 29.96069760513793 31.918831445104484 19.355701898991633 32-33 19.665598508291055 29.962634968049056 32.35591563523695 18.01585088842294 34-35 16.952791060420832 31.626134032722764 31.050905936931695 20.370168969924713 36-37 18.121803111234673 29.74352035800661 31.756927632270692 20.377748898488026 38-39 19.35126353054273 28.471151388376313 32.537706391776226 19.639878689304734 40-41 20.91155798520292 29.30417977084756 31.22533012956592 18.5589321143836 42-43 20.366997919347725 31.198034825240807 30.11254343978141 18.322423815630064 44-45 19.162512006002643 30.738502676090253 30.832224039616797 19.26676127829031 46-47 19.056466046586337 29.794475781619152 31.099668326564778 20.049389845229733 48-49 20.611818505201356 29.97035194729757 30.120707649645855 19.29712189785522 50-51 19.83230155097723 30.34509443461551 30.905058832809555 18.9175451815977 52-53 18.667856729263427 31.088374478485786 32.607067556681784 17.636701235569006 54-55 19.658718038971926 30.804312344120042 31.510438905575977 18.02653071133205 56-57 20.94156752884787 28.768522191707806 30.85919494015481 19.430715339289517 58-59 19.64443187832526 29.609730777195914 29.94376791058886 20.802069433889965 60-61 21.235896752797967 29.800149329273395 30.387028562937846 18.576925354990788 62-63 17.4988363930956 34.849087975774474 29.64798905318604 18.004086577943884 64-65 16.945512293465946 32.98798941332077 31.301378484475812 18.765119808737467 66-67 18.280907804745038 28.96140034332561 32.97948162381146 19.77821022811789 68-69 21.01203775317698 31.25741381835518 28.616915849726222 19.113632578741623 70-71 19.015764859909535 33.39670554880718 28.669526665981376 18.918002925301916 72-73 18.179681666025736 32.2630348780921 31.255594252277913 18.30168920360425 74-75 19.055803635077584 30.343070310268892 31.38573065899799 19.21539539565553 76-77 19.77624005146204 30.432619497357138 30.591446677155826 19.199693774024993 78-79 19.32490729744424 32.360226684447895 29.17856828175453 19.136297736353335 80-81 18.66939335754291 32.39227550740173 31.582791132199628 17.355540002855737 82-83 17.782146092831987 32.34845933970498 31.781067274508207 18.088327292954826 84-85 18.6352061253451 31.67425244745513 30.815331172905086 18.87521025429468 86-87 19.909581685937717 31.878286914989218 29.073511333534707 19.138620065538355 88-89 18.441212597596266 31.893598199576118 30.51423593768563 19.150953265141986 90-91 18.61660935670986 31.90445261401203 30.13011807389533 19.34881995538278 92-93 17.59316796419655 32.3672714431439 30.407763716594165 19.631796876065387 94-95 17.240713779785512 32.21787066686827 29.908232595977303 20.633182957368916 96-97 18.349189720734298 30.063894610505553 31.463492834137902 20.123422834622247 98-99 19.612282159418562 31.006950831062845 29.939483231202836 19.44128377831576 100 20.052880962930946 31.86686007471472 29.770391725222144 18.309867237132188 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1085.0 1 2210.0 2 3390.5 3 3906.5 4 5345.5 5 7912.0 6 10191.5 7 12783.0 8 16971.5 9 22164.0 10 28584.0 11 36358.0 12 45714.0 13 57200.0 14 71736.5 15 89586.0 16 110936.5 17 136787.0 18 169715.0 19 211618.0 20 262940.5 21 327113.5 22 407736.0 23 507524.5 24 631797.5 25 787031.0 26 975955.0 27 1200840.0 28 1471504.0 29 1807826.5 30 2239622.0 31 2809941.5 32 3591469.0 33 4605886.0 34 5756436.0 35 6872111.0 36 7618122.0 37 7539478.5 38 6640313.5 39 5456340.5 40 4437229.5 41 3710726.5 42 3217064.0 43 2874210.0 44 2606112.0 45 2375766.5 46 2179699.5 47 1997784.0 48 1826144.0 49 1658299.5 50 1481093.5 51 1302433.5 52 1133951.0 53 975631.0 54 821188.5 55 678675.5 56 547881.0 57 423321.5 58 317328.5 59 231947.5 60 165187.5 61 117179.0 62 82958.5 63 58005.5 64 40228.5 65 28064.0 66 19937.0 67 14940.5 68 11488.5 69 8784.0 70 7124.0 71 5989.0 72 5067.5 73 4526.0 74 3860.0 75 2914.0 76 2329.5 77 2046.0 78 1675.0 79 1237.5 80 940.5 81 764.0 82 614.5 83 476.0 84 356.5 85 238.5 86 176.5 87 133.5 88 85.5 89 50.5 90 27.0 91 18.0 92 14.0 93 7.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012304777044046212 2 0.00466317457141806 3 0.018289201428342523 4 0.08562495213149707 5 0.01158799391526682 6 0.010736431052870792 7 0.00599259285300035 8 0.003707463733045539 9 0.0061947624534253055 10-11 0.005076703299560017 12-13 0.007959662222791656 14-15 0.008988378750206522 16-17 0.003409825154642131 18-19 0.00531001518691912 20-21 0.014124303447870818 22-23 0.014853339279706269 24-25 0.0025536675286000807 26-27 0.004689722094706186 28-29 0.006543964490522958 30-31 0.02665932920351212 32-33 0.024062266684921833 34-35 0.012414030312962728 36-37 0.016137830983416344 38-39 0.004840328236436898 40-41 0.018019131431815244 42-43 0.029951732620533696 44-45 0.007548686141119711 46-47 0.013056786694111769 48-49 0.0028696851615875756 50-51 0.025079751567868645 52-53 0.01130465015709548 54-55 0.013334514630049081 56-57 0.005843518299151644 58-59 0.045655613704047984 60-61 0.03997699636685912 62-63 0.0895800225721338 64-65 0.026201894956085957 66-67 0.021048101733131572 68-69 0.006559280369343031 70-71 0.003828969705018114 72-73 0.008553918321010465 74-75 0.0059481768044221395 76-77 0.006097761887564847 78-79 0.027407254619225656 80-81 0.019280138788001208 82-83 0.006481679916654663 84-85 0.0047729383696285795 86-87 0.0033904250414700395 88-89 0.0029181854445178056 90-91 0.002341287342295076 92-93 0.0049924659660496185 94-95 0.0017449891269002552 96-97 7.683465874736349E-4 98-99 0.0033822565727660006 100 2.5424358841320274E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9.7937573E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.6662024168105 #Duplication Level Percentage of deduplicated Percentage of total 1 92.79473645625221 69.28630575457113 2 4.128463643975193 6.165134042229898 3 1.231116207698627 2.7576831588792547 4 0.5703481231935297 1.7034291365766423 5 0.3224458187003536 1.2037902383767394 6 0.2050897975014951 0.9187965800321585 7 0.13305295887996452 0.6954191411920897 8 0.09733549619680588 0.5814137489097119 9 0.06712459392186332 0.451074466522445 >10 0.39471063772382897 5.355720796802967 >50 0.030901325615392786 1.5962788528127478 >100 0.02112373337610166 3.0720597899118514 >500 0.001919286229984008 0.9847883957735886 >1k 0.0013682816005865252 1.9855534909137167 >5k 1.4820885583076874E-4 0.7706729997125056 >10k+ 1.1543027792747202E-4 2.4718794067823424 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 270692 0.2763923913042035 No Hit GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG 221970 0.2266443747794322 No Hit AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA 192067 0.19611165982232376 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0022238656046745207 0.0 0.0 1.1231644468053134E-5 0.0 2 0.002252455245138656 9.189527292043473E-6 0.0 2.2463288936106267E-5 0.0 3 0.002347413693823105 1.1231644468053134E-5 0.0 2.34843475241111E-5 0.0 4 0.0026812998521006846 1.2252703056057964E-5 0.0 3.7779167756178725E-5 0.0 5 0.00279667947254523 1.2252703056057964E-5 0.0 3.982128493218838E-5 0.0 6 0.0029549435536859792 1.3273761644062796E-5 0.0 4.9010812224231855E-5 0.0 7 0.0030560283538984575 1.8379054584086947E-5 0.0 5.7179280928270504E-5 1.0210585880048304E-6 8 0.0034573043789843556 2.0421171760096607E-5 0.0 7.555833551235745E-5 1.0210585880048304E-6 9 0.0036104631671850804 2.0421171760096607E-5 0.0 8.474786280440092E-5 1.0210585880048304E-6 10-11 0.004267003839272187 2.144223034810144E-5 0.0 9.597950727245406E-5 1.0210585880048304E-6 12-13 0.005221693619056703 2.2463288936106267E-5 1.0210585880048304E-6 1.1027432750452168E-4 1.5315878820072457E-6 14-15 0.006331584304217953 2.2973818230108683E-5 1.0210585880048304E-6 1.2201650126657723E-4 2.552646470012076E-6 16-17 0.007665597349446265 2.34843475241111E-5 2.0421171760096608E-6 1.409060851446666E-4 3.5737050580169065E-6 18-19 0.008991952455264539 2.450540611211593E-5 2.0421171760096608E-6 1.5366931749472696E-4 4.594763646021737E-6 20-21 0.010390802720831156 3.0121228346142496E-5 2.0421171760096608E-6 1.6285884478677045E-4 5.105292940024152E-6 22-23 0.011603309794086893 3.165281622814974E-5 2.552646470012076E-6 1.74601018548826E-4 5.105292940024152E-6 24-25 0.012648363258909837 3.216334552215216E-5 3.5737050580169065E-6 1.878747801928888E-4 6.126351528028982E-6 26-27 0.01359386351140231 3.3694933404159404E-5 4.0842343520193216E-6 1.9706430748493226E-4 6.126351528028982E-6 28-29 0.014582248224590985 3.573705058016906E-5 4.0842343520193216E-6 2.118696570110023E-4 7.147410116033813E-6 30-31 0.015465974432509164 3.726863846217631E-5 4.0842343520193216E-6 2.2565394794906753E-4 7.657939410036229E-6 32-33 0.01631447411914118 3.828969705018114E-5 4.0842343520193216E-6 2.3382241665310615E-4 8.168468704038643E-6 34-35 0.01723342684834553 3.982128493218838E-5 4.0842343520193216E-6 2.5475411770720514E-4 8.168468704038643E-6 36-37 0.018141658462375823 4.1352872814195626E-5 6.126351528028982E-6 2.813016409953308E-4 8.168468704038643E-6 38-39 0.019149953818030592 4.2373931402200465E-5 6.126351528028982E-6 3.0070175416742253E-4 9.189527292043473E-6 40-41 0.020416576996450587 4.390551928420771E-5 6.126351528028982E-6 3.1857027945750706E-4 9.189527292043473E-6 42-43 0.021835337904483297 4.4926577872212535E-5 6.126351528028982E-6 3.28270336043553E-4 9.189527292043473E-6 44-45 0.023197940590175742 4.798975363622703E-5 6.636880822031397E-6 3.4205462698161816E-4 9.189527292043473E-6 46-47 0.024671838661960717 5.0542400106239107E-5 7.147410116033813E-6 3.5839156438969547E-4 9.189527292043473E-6 48-49 0.026417848847448976 5.258451728224877E-5 7.147410116033813E-6 3.691126795637462E-4 9.189527292043473E-6 50-51 0.028613635340953365 5.462663445825842E-5 7.147410116033813E-6 3.8800226344183554E-4 9.189527292043473E-6 52-53 0.030943691038780387 5.615822234026567E-5 7.147410116033813E-6 4.0536025943791766E-4 9.189527292043473E-6 54-55 0.033366152538821844 5.7179280928270504E-5 7.147410116033813E-6 4.2476037261000946E-4 9.189527292043473E-6 56-57 0.03668816665489556 5.871086881027775E-5 7.147410116033813E-6 4.4467101507610367E-4 9.189527292043473E-6 58-59 0.04035683016159691 6.279510316229708E-5 7.147410116033813E-6 4.6151848177818333E-4 1.0210585880048303E-5 60-61 0.04427361090518345 7.760045268836711E-5 7.147410116033813E-6 4.7581330201025096E-4 1.1231644468053134E-5 62-63 0.04929824021675522 8.117415774638401E-5 7.657939410036229E-6 4.987871202403597E-4 1.2252703056057964E-5 64-65 0.05478643012728118 8.627945068640817E-5 8.168468704038643E-6 5.17676704118449E-4 1.2252703056057964E-5 66-67 0.061551964331401185 9.802162444846372E-5 9.70005658604589E-6 5.360557587025359E-4 1.3273761644062796E-5 68-69 0.07010741424029365 1.1282697397453376E-4 1.0210585880048303E-5 5.559664011686302E-4 1.3273761644062796E-5 70-71 0.08100619360865721 1.1486909115054341E-4 1.0210585880048303E-5 5.917034517487992E-4 1.3273761644062796E-5 72-73 0.09359227229369876 1.1844279620856032E-4 1.0210585880048303E-5 6.294826195049779E-4 1.3273761644062796E-5 74-75 0.10885505606719496 1.2048491338456998E-4 1.0210585880048303E-5 6.662407286731518E-4 1.3273761644062796E-5 76-77 0.12838688579713936 1.2405861844258688E-4 1.0210585880048303E-5 7.024883085473234E-4 1.378429093806521E-5 78-79 0.1532950995222232 1.2661126491259896E-4 1.1231644468053134E-5 7.223989510134175E-4 1.5315878820072454E-5 80-81 0.1840989055344469 1.276323235006038E-4 1.174217376205555E-5 7.652834117096205E-4 1.582640811407487E-5 82-83 0.22243301863320628 1.2865338208860864E-4 1.4294820232067626E-5 8.035731087598015E-4 1.6336937408077286E-5 84-85 0.2750726730792073 1.2916391138261105E-4 1.4294820232067626E-5 8.413522765159802E-4 1.6336937408077286E-5 86-87 0.34698123466874153 1.2967444067661345E-4 1.4294820232067626E-5 8.847472665061856E-4 1.6336937408077286E-5 88 0.4112619780765856 1.2967444067661345E-4 1.4294820232067626E-5 9.107842605003087E-4 1.6336937408077286E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 120260 0.0 66.895485 1 GCGTGTT 137130 0.0 57.06783 2 CGTGTTT 196400 0.0 40.975258 3 CGGGAAT 15080 0.0 39.61626 1 GGTAAAC 46755 0.0 37.437145 1 CGCTTGT 15245 0.0 36.31785 1 CCGCGTG 5340 0.0 36.029076 1 GTAAACT 91915 0.0 34.664227 2 GCGGGTT 8380 0.0 34.297962 1 TAACTGA 120120 0.0 33.8129 4 CGCGTTT 12780 0.0 33.752785 1 CGGCGAG 13960 0.0 31.742167 1 TAAACTG 115750 0.0 31.219286 3 CCGGAAT 9370 0.0 30.222334 1 CTGGAAT 362285 0.0 29.884817 1 GATAACT 93710 0.0 29.07136 2 GGATAAC 50880 0.0 28.99346 1 CGGGTTT 20485 0.0 27.94639 1 ATAACTG 102065 0.0 27.833847 3 GCGCGTT 5375 0.0 26.167597 1 >>END_MODULE