FastQCFastQC Report
Fri 27 May 2016
SRR1233068_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233068_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44641464
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT570110.1277086253264454No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA499160.11181532935389395No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT485580.10877331442355923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT350100.038.8439641
GCGTGTT412800.031.1816462
GTTTTTT4175150.030.3454021
CGCGTTT18850.022.1557581
CGTGTTT570650.022.1360653
CTGGAAT755350.020.995541
CTCGTAT31350.020.458361
CGGCGAG28950.017.2598151
CGCCGTA43550.017.23834446-47
CTCGCGT64450.016.7785741
TATGGCG815350.016.4283547
TGGCGAG797400.016.3027299
CGGACGT19050.016.0508791
ATGGCGA840100.015.9620248
CGCTTCG36750.014.6007693
TCGCGTC74800.014.3983482
CGCGTGC19750.014.3491581
GCGCGTT6400.013.983351
CTAACGA38650.013.96953394-95
GTCGCCG56250.013.87251144-45
Produced by FastQC (version 0.11.5)