FastQCFastQC Report
Fri 27 May 2016
SRR1233068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233068_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44641464
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT466280.10444997950784052No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA456900.10234879393740312No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC453840.10166333254662079No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT453190.10151772800282716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT357750.045.9186481
GCGTGTT408300.038.2911072
ATTCGAC45750.029.8530561
CGATAGT35000.028.954852
CGCGTTT23150.028.1622621
CGTGTTT558900.027.4539223
GTTTTTT3773650.026.1733361
CCGATAG42400.023.232851
CTGGAAT933500.022.0327361
CGTCTCA67800.020.4249631
TTCGACT80650.019.939862
CGATTCA52150.018.3417051
TTCGTAT70800.017.4086251
CGGGTTT103250.017.0994381
CGGCGAG35500.016.7563571
GCGGGTT44150.016.3836561
TATGGCG1101950.016.338827
CGGGGAT42750.016.2522951
CGGACGT19350.016.2315861
TCGACTC90300.016.2282523
Produced by FastQC (version 0.11.5)