##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233068_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 44641464 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.759900974573775 31.0 31.0 34.0 27.0 34.0 2 31.384707858147305 31.0 31.0 34.0 28.0 34.0 3 29.59675614581099 31.0 30.0 34.0 16.0 34.0 4 32.16351352186837 35.0 32.0 37.0 19.0 37.0 5 34.100488348679605 35.0 33.0 37.0 28.0 37.0 6 34.95817280992397 36.0 35.0 37.0 32.0 37.0 7 35.3740192301937 37.0 35.0 37.0 33.0 37.0 8 35.50655316770077 37.0 35.0 37.0 33.0 37.0 9 37.20700425505758 39.0 37.0 39.0 33.0 39.0 10-11 37.169208821198154 39.0 37.0 39.0 33.0 39.0 12-13 37.182663588273 39.0 37.0 39.0 33.0 39.0 14-15 38.447405208305895 40.0 38.0 41.0 33.5 41.0 16-17 38.336598302421265 40.0 38.0 41.0 33.0 41.0 18-19 38.24421243219084 40.0 37.5 41.0 33.0 41.0 20-21 38.09252521377883 40.0 37.0 41.0 33.0 41.0 22-23 37.96280500567813 40.0 37.0 41.0 33.0 41.0 24-25 37.77039692291453 40.0 37.0 41.0 32.5 41.0 26-27 37.522816680026445 40.0 37.0 41.0 32.0 41.0 28-29 37.42323521244734 40.0 37.0 41.0 32.0 41.0 30-31 37.3171954665286 40.0 37.0 41.0 32.0 41.0 32-33 37.211787241117364 40.0 37.0 41.0 31.0 41.0 34-35 37.07716115672192 40.0 37.0 41.0 31.0 41.0 36-37 36.96608039109111 40.0 37.0 41.0 31.0 41.0 38-39 36.846969288462404 40.0 37.0 41.0 30.0 41.0 40-41 36.86594213173654 40.0 37.0 41.0 30.0 41.0 42-43 36.847011569781856 40.0 37.0 41.0 30.0 41.0 44-45 36.746372453197324 40.0 36.0 41.0 30.0 41.0 46-47 36.62907178626579 40.0 36.0 41.0 30.0 41.0 48-49 36.53468729206551 40.0 36.0 41.0 30.0 41.0 50-51 36.43700395892034 39.5 36.0 41.0 30.0 41.0 52-53 36.29461933640886 39.0 36.0 41.0 30.0 41.0 54-55 36.14241271298809 39.0 35.0 41.0 29.0 41.0 56-57 35.969183369523904 39.0 35.0 41.0 28.5 41.0 58-59 35.7518452015821 39.0 35.0 40.0 28.0 41.0 60-61 35.50740940082073 38.5 35.0 40.0 28.0 41.0 62-63 35.25166539117086 38.0 34.0 40.0 27.5 41.0 64-65 34.96694883259205 38.0 34.0 40.0 27.0 41.0 66-67 34.64579649538375 37.5 34.0 40.0 26.0 41.0 68-69 34.31197008906339 37.0 34.0 40.0 26.0 41.0 70-71 33.98289808551081 36.5 33.0 39.0 26.0 41.0 72-73 33.61584547272016 36.0 33.0 39.0 26.0 40.0 74-75 33.204472516851155 35.5 32.5 38.5 25.5 40.0 76-77 32.35288586189736 35.0 31.5 37.0 24.5 39.0 78-79 32.52219854393664 35.0 32.0 37.0 25.0 39.0 80-81 32.28685659816175 35.0 32.0 37.0 25.0 39.0 82-83 31.938162959888594 35.0 32.0 36.0 24.0 38.5 84-85 31.57483061039396 35.0 32.0 36.0 23.0 37.0 86-87 31.23040083318056 34.5 31.0 35.5 22.0 37.0 88-89 30.909515333099293 34.0 31.0 35.0 20.5 36.5 90-91 30.593261065542116 34.0 31.0 35.0 19.5 36.0 92-93 30.272557011123112 34.0 31.0 35.0 18.0 36.0 94-95 29.936169174469725 34.0 31.0 35.0 11.0 35.5 96-97 29.556809438418057 34.0 30.0 35.0 2.0 35.0 98-99 29.127658190152545 34.0 30.0 35.0 2.0 35.0 100-101 27.494897938383026 32.5 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.8237411295498624 1101 2 1.4114367965884824 1101 3 3.3001001059483386 1101 4 4.093427017784727 1101 5 1.9604911245821697 1101 6 1.0368284540701467 1101 7 0.6650527585622825 1101 8 0.5030975856388267 1101 9 0.5261611939035546 1101 10-11 0.46072777494966033 1101 12-13 0.454136993129687 1101 14-15 0.5457079419194955 1101 16-17 0.5524619455995321 1101 18-19 0.511735473566219 1101 20-21 0.5167208029130066 1101 22-23 0.5464956718350393 1101 24-25 0.5546431909474236 1101 26-27 0.5990343605848452 1101 28-29 0.5438318911584474 1101 30-31 0.5219139024176584 1101 32-33 0.5116167682670323 1101 34-35 0.5226234988564826 1101 36-37 0.5614786609409563 1101 38-39 0.5868024804832501 1101 40-41 0.5105681344221367 1101 42-43 0.49677865984924097 1101 44-45 0.42371647164944903 1101 46-47 0.4559603773791707 1101 48-49 0.48921551024071874 1101 50-51 0.5309966217703632 1101 52-53 0.5897379666180385 1101 54-55 0.5835943135654205 1101 56-57 0.5647406799705337 1101 58-59 0.5165034783653937 1101 60-61 0.5016486571261254 1101 62-63 0.5146016114485548 1101 64-65 0.5241011489330205 1101 66-67 0.5030533075490808 1101 68-69 0.47633205939941803 1101 70-71 0.5521606975311428 1101 72-73 0.6134856413783396 1101 74-75 0.5877109563904952 1101 76-77 0.4542573893091628 1101 78-79 0.5579914258656018 1101 80-81 0.5696588553493385 1101 82-83 0.5596865770374038 1101 84-85 0.5924079453134148 1101 86-87 0.5847744080385873 1101 88-89 0.6508770311234109 1101 90-91 0.6896595072805205 1101 92-93 0.6829399426353895 1101 94-95 0.6854672530296675 1101 96-97 0.6512917503766644 1101 98-99 0.7599386974518154 1101 100-101 1.1878974487249785 1102 1 1.6872306741776448 1102 2 1.3204616984319628 1102 3 3.2164736784886117 1102 4 4.03318660158061 1102 5 1.9152596558004973 1102 6 0.9943497768134009 1102 7 0.6321422608745451 1102 8 0.5093203527010743 1102 9 0.49632004353041737 1102 10-11 0.428007015643324 1102 12-13 0.3904986249232323 1102 14-15 0.5195417831528104 1102 16-17 0.5175122157547207 1102 18-19 0.4588981764779305 1102 20-21 0.503506110187466 1102 22-23 0.4937888041994114 1102 24-25 0.5390743188949116 1102 26-27 0.5399541655869484 1102 28-29 0.48529800212328666 1102 30-31 0.4710071758512626 1102 32-33 0.49617499607625604 1102 34-35 0.5092821808750401 1102 36-37 0.5085007303746281 1102 38-39 0.5350175703761693 1102 40-41 0.5314544744630396 1102 42-43 0.549054386937236 1102 44-45 0.5093192176431955 1102 46-47 0.4801985518057421 1102 48-49 0.475450973008968 1102 50-51 0.5386362289920399 1102 52-53 0.5122642703599993 1102 54-55 0.5902343872553217 1102 56-57 0.5943757054609051 1102 58-59 0.6084265628202914 1102 60-61 0.5174130478810781 1102 62-63 0.5026969274197484 1102 64-65 0.4768990421871351 1102 66-67 0.5083394284522882 1102 68-69 0.4984137068276553 1102 70-71 0.5352739112347535 1102 72-73 0.5835130104896322 1102 74-75 0.5649948722666167 1102 76-77 0.47836940153551666 1102 78-79 0.5240123310974738 1102 80-81 0.575775175253618 1102 82-83 0.5751782531913747 1102 84-85 0.5793312379333706 1102 86-87 0.5067939472036791 1102 88-89 0.5431175155783627 1102 90-91 0.6458717064879202 1102 92-93 0.6061297453300902 1102 94-95 0.6986190331821653 1102 96-97 0.7047628121523353 1102 98-99 0.774903641983574 1102 100-101 1.211614603599518 1103 1 1.738056795329097 1103 2 1.3298198083003 1103 3 3.2014262226568775 1103 4 4.0708889904463135 1103 5 1.9271975483261556 1103 6 0.9758855025818036 1103 7 0.6399750059457077 1103 8 0.439219593848307 1103 9 0.41733999470815064 1103 10-11 0.36584519587596276 1103 12-13 0.33134624924865363 1103 14-15 0.47956666477340804 1103 16-17 0.43455191708831364 1103 18-19 0.42234212600445176 1103 20-21 0.4416037033364404 1103 22-23 0.4128693189126338 1103 24-25 0.45367176542064414 1103 26-27 0.5176666883898307 1103 28-29 0.4875697487028816 1103 30-31 0.4344490062419837 1103 32-33 0.4510480454847823 1103 34-35 0.4732265377199596 1103 36-37 0.4091503858713921 1103 38-39 0.47292951728651644 1103 40-41 0.4638202659088151 1103 42-43 0.44134659867937387 1103 44-45 0.46464207461384177 1103 46-47 0.41447002041610403 1103 48-49 0.3946282472192806 1103 50-51 0.4538619393566634 1103 52-53 0.502898926591719 1103 54-55 0.5853964433435621 1103 56-57 0.5737610406030456 1103 58-59 0.4910904605433615 1103 60-61 0.44178659427057454 1103 62-63 0.3807236533093956 1103 64-65 0.421506615347667 1103 66-67 0.43355550449491886 1103 68-69 0.505628073509719 1103 70-71 0.5293816103160509 1103 72-73 0.5165646698385657 1103 74-75 0.5467705420487405 1103 76-77 0.4731443197948977 1103 78-79 0.5532951122789385 1103 80-81 0.5237653664830049 1103 82-83 0.5690462869460333 1103 84-85 0.550172518300986 1103 86-87 0.5100091440555907 1103 88-89 0.6011036718065164 1103 90-91 0.6267523878465404 1103 92-93 0.6599571564500195 1103 94-95 0.6508952841372384 1103 96-97 0.6851287161703645 1103 98-99 0.7018647938656706 1103 100-101 1.1570980275699654 1104 1 1.677392536405403 1104 2 1.2790340231964095 1104 3 3.139507031748707 1104 4 4.015006426006288 1104 5 1.853947643985741 1104 6 0.9103785041727406 1104 7 0.5154067308422512 1104 8 0.4048993950512525 1104 9 0.3843005162909776 1104 10-11 0.2748385672487643 1104 12-13 0.25177868359491384 1104 14-15 0.32925829594650935 1104 16-17 0.30718147850363664 1104 18-19 0.2960547735433039 1104 20-21 0.3108893222659219 1104 22-23 0.2973178354011168 1104 24-25 0.3511189401068364 1104 26-27 0.41058420092240056 1104 28-29 0.3808101679151292 1104 30-31 0.32385874646644197 1104 32-33 0.3276618277181669 1104 34-35 0.3212908805353081 1104 36-37 0.30131770640584676 1104 38-39 0.3706466254649996 1104 40-41 0.3665214509769896 1104 42-43 0.38168375393304643 1104 44-45 0.3556506532206001 1104 46-47 0.3173107211528219 1104 48-49 0.32244164430979083 1104 50-51 0.3824446370090655 1104 52-53 0.4105021265754232 1104 54-55 0.4195083746452468 1104 56-57 0.44148178007073824 1104 58-59 0.4522525993742903 1104 60-61 0.3780830492793186 1104 62-63 0.3406539773408781 1104 64-65 0.34015316759986547 1104 66-67 0.32225091684033913 1104 68-69 0.4393865797237453 1104 70-71 0.4673129849096611 1104 72-73 0.45211496755256064 1104 74-75 0.47891711866244435 1104 76-77 0.380538674223196 1104 78-79 0.3737467189096222 1104 80-81 0.38148252342301703 1104 82-83 0.40411931026977754 1104 84-85 0.4396140859348314 1104 86-87 0.4878584251678326 1104 88-89 0.5008813015883717 1104 90-91 0.4841873173865814 1104 92-93 0.4289035107162995 1104 94-95 0.4254586894279697 1104 96-97 0.5156200784205929 1104 98-99 0.5392116744855038 1104 100-101 1.011521575299696 1105 1 1.7080852540034748 1105 2 1.3735101439902024 1105 3 3.0539214913347195 1105 4 4.012503355866713 1105 5 1.9263569437316477 1105 6 0.9925757058716513 1105 7 0.6364628935689041 1105 8 0.4862954098866581 1105 9 0.4613976607526169 1105 10-11 0.46113153184650457 1105 12-13 0.4106946620514833 1105 14-15 0.5213289373125534 1105 16-17 0.4835721340941461 1105 18-19 0.4681876353762249 1105 20-21 0.5122726882034172 1105 22-23 0.543560867804203 1105 24-25 0.5598434976750397 1105 26-27 0.5627623126014285 1105 28-29 0.5447415433530836 1105 30-31 0.5484928753104796 1105 32-33 0.5272330977685655 1105 34-35 0.5543831282014651 1105 36-37 0.559058961136401 1105 38-39 0.6078088811844893 1105 40-41 0.6360386291021669 1105 42-43 0.6127155657473793 1105 44-45 0.5761835326412594 1105 46-47 0.5774176418491095 1105 48-49 0.6114368524236014 1105 50-51 0.5934818755650824 1105 52-53 0.6713922992746362 1105 54-55 0.6508143198393981 1105 56-57 0.6099727563288369 1105 58-59 0.6803466135277816 1105 60-61 0.63992622840113 1105 62-63 0.6479871464963196 1105 64-65 0.5672734441746954 1105 66-67 0.6515055478913325 1105 68-69 0.7430030865065973 1105 70-71 0.7429313422615635 1105 72-73 0.7561213164932141 1105 74-75 0.7198464182849236 1105 76-77 0.6352938710901128 1105 78-79 0.6831075879751083 1105 80-81 0.6674186325070792 1105 82-83 0.7179631230900085 1105 84-85 0.7404910427631144 1105 86-87 0.6914333153602037 1105 88-89 0.7366375028074792 1105 90-91 0.7584031397134403 1105 92-93 0.8119077136448105 1105 94-95 0.7756420010594773 1105 96-97 0.9135356233549423 1105 98-99 0.9656502527948909 1105 100-101 1.355856820656303 1106 1 1.67239458651434 1106 2 1.270105968788556 1106 3 3.138641728561481 1106 4 4.019993521169326 1106 5 1.8561829383683488 1106 6 0.9309275221322295 1106 7 0.5055287334376857 1106 8 0.35853678896888397 1106 9 0.3333543673059438 1106 10-11 0.2920445022177631 1106 12-13 0.21549916972474392 1106 14-15 0.294238220167621 1106 16-17 0.3183186027042879 1106 18-19 0.2846948648473173 1106 20-21 0.3036875242205568 1106 22-23 0.2964490484146509 1106 24-25 0.2751246643436005 1106 26-27 0.317699857361994 1106 28-29 0.283274286461193 1106 30-31 0.2227398492219237 1106 32-33 0.2430312840055464 1106 34-35 0.2747995390026645 1106 36-37 0.2991523316304665 1106 38-39 0.28156512353272234 1106 40-41 0.2643565017286065 1106 42-43 0.28508518771865 1106 44-45 0.22428903007311618 1106 46-47 0.24037531881413088 1106 48-49 0.3099643158033487 1106 50-51 0.31500204081712013 1106 52-53 0.3452849391135189 1106 54-55 0.3674074585473477 1106 56-57 0.3313853649656622 1106 58-59 0.3387303238265673 1106 60-61 0.2681683222902933 1106 62-63 0.29086717556842245 1106 64-65 0.3006115941106131 1106 66-67 0.27559803205100764 1106 68-69 0.368143109830676 1106 70-71 0.40609948132048856 1106 72-73 0.39578930197745166 1106 74-75 0.3878037578164637 1106 76-77 0.32176047353149784 1106 78-79 0.37649989480272694 1106 80-81 0.37741750898346993 1106 82-83 0.43959577187055743 1106 84-85 0.4773154500725916 1106 86-87 0.3815941080251868 1106 88-89 0.3840821974948554 1106 90-91 0.37179523828681127 1106 92-93 0.4480375350231789 1106 94-95 0.48486724664372005 1106 96-97 0.5289760958075611 1106 98-99 0.6175859265162451 1106 100-101 1.0668116050787937 1107 1 0.8798059942696064 1107 2 0.6882542907322673 1107 3 1.114678881277733 1107 4 -2.0474389638109862 1107 5 -0.4890490903359108 1107 6 -0.1428867157097642 1107 7 -0.0011830338222864611 1107 8 0.018932121537929447 1107 9 0.15231563690864647 1107 10-11 0.08420644087352969 1107 12-13 0.10668219506005272 1107 14-15 0.10634652112438658 1107 16-17 0.1518970640997992 1107 18-19 0.147589178923063 1107 20-21 0.2358360277312741 1107 22-23 0.21303253746761186 1107 24-25 0.20083347600585455 1107 26-27 0.1771649894316596 1107 28-29 0.18678221568340803 1107 30-31 0.16095865128836806 1107 32-33 0.12950187233651178 1107 34-35 0.06521838297388882 1107 36-37 -0.015978056759244907 1107 38-39 0.12290176431338296 1107 40-41 0.0638585825820428 1107 42-43 0.07044871159872201 1107 44-45 0.10119772840339891 1107 46-47 0.10578641589259519 1107 48-49 0.12549475608096117 1107 50-51 0.17586837568178026 1107 52-53 0.09874946426548803 1107 54-55 0.15034974860337513 1107 56-57 0.20002101986595733 1107 58-59 0.13453294402346927 1107 60-61 0.08819250323350047 1107 62-63 0.09616386855823578 1107 64-65 0.13215726121127602 1107 66-67 0.07624014935406365 1107 68-69 0.14608104421017032 1107 70-71 0.1376441021234882 1107 72-73 0.18770511019105385 1107 74-75 0.23556227227711446 1107 76-77 0.16338234652019423 1107 78-79 0.1678451983423983 1107 80-81 0.2610470167325474 1107 82-83 0.2755162534521247 1107 84-85 0.27889455152737597 1107 86-87 0.29328553988230865 1107 88-89 0.30862413657556687 1107 90-91 0.3018927893789609 1107 92-93 0.2813812563070499 1107 94-95 0.3449728474775071 1107 96-97 0.3183639110349752 1107 98-99 0.3648268017830638 1107 100-101 0.8269465738164286 1108 1 0.6151770595383255 1108 2 0.5689831084519668 1108 3 -1.9619872557433666 1108 4 -2.1874878068494787 1108 5 -0.7993510923906086 1108 6 -0.31907754728452886 1108 7 -0.09567127278107534 1108 8 -0.0335169712745369 1108 9 0.04002946115118533 1108 10-11 0.04985179474668655 1108 12-13 0.043107770944310175 1108 14-15 0.062312725709936956 1108 16-17 0.08279622946743359 1108 18-19 0.06956316170568044 1108 20-21 0.10205306251823743 1108 22-23 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-1.6543878100088065 2316 84-85 -1.6691792581390708 2316 86-87 -1.6789834568975586 2316 88-89 -1.710165208852704 2316 90-91 -1.6693054767239843 2316 92-93 -1.780950364236169 2316 94-95 -1.8172158598180168 2316 96-97 -1.8121071957539279 2316 98-99 -1.9454381334940436 2316 100-101 -2.312110810888843 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7698.0 3 707.0 4 2029.0 5 5082.0 6 14197.0 7 41193.0 8 110600.0 9 210497.0 10 282197.0 11 230881.0 12 162700.0 13 123489.0 14 109274.0 15 107121.0 16 109626.0 17 115211.0 18 123762.0 19 133824.0 20 145892.0 21 162522.0 22 186716.0 23 220877.0 24 267511.0 25 330532.0 26 417995.0 27 526752.0 28 662711.0 29 834503.0 30 1042718.0 31 1297221.0 32 1615527.0 33 2039308.0 34 2651640.0 35 3612359.0 36 5323716.0 37 8596459.0 38 1.0402118E7 39 2412412.0 40 1887.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.472394134957707 16.561096968880314 12.721830463483924 44.24467843267805 2 16.587595904147197 20.148694549513717 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30.075726190667666 19.797599791868283 46-47 20.62062381273129 29.152633100806625 29.921986865396665 20.304756221065425 48-49 20.908515638375675 29.394182977952344 29.32902489935751 20.36827648431447 50-51 20.364148451762247 29.237234468833726 30.175934642286823 20.222682437117207 52-53 20.471076433532005 29.402468810356236 30.166301346704373 19.960153409407383 54-55 20.319320164728246 29.66895137311154 29.962543179506962 20.04918528265325 56-57 20.914723974019747 28.981993461961185 29.712942155883955 20.390340408135113 58-59 20.869537839360174 29.030020701185787 29.22625651341172 20.87418494604232 60-61 21.226703133414198 29.20254964806746 29.30992094842756 20.26082627009079 62-63 20.167262413871818 30.36187018059452 29.37435896815529 20.096508437378375 64-65 20.187148620245914 29.775374046592518 29.715764077244383 20.32171325591718 66-67 20.363551052884333 29.438529346585057 29.77842033163121 20.4194992688994 68-69 21.146643928654196 29.703197277074544 28.93506693019124 20.21509186408002 70-71 20.723030391676268 29.96304309425807 28.89761757372825 20.416308940337412 72-73 20.04228828213657 30.55117985709445 29.112345367766938 20.294186493002037 74-75 20.2135152993705 29.37255687964715 30.081764536525846 20.332163284456506 76-77 20.796542881189055 29.62617747348759 29.132157913057082 20.445121732266276 78-79 20.466726191298886 30.38064635112877 28.666183050497246 20.486444407075098 80-81 20.465284248250835 29.800133873620084 29.786286848160632 19.948295029968456 82-83 20.467837862039694 30.0979980982911 29.241336424557662 20.192827615111547 84-85 20.346933694660017 30.151387236586512 29.138243879920285 20.363435188833183 86-87 20.81240100011057 30.191923141230316 28.63744231147975 20.358233547179367 88-89 20.506458082405835 30.052758014978608 29.114571927060588 20.326211975554966 90-91 20.458939220092162 30.449550145875175 28.847483635594518 20.24402699843815 92-93 20.321211301642915 30.660078753119702 28.75973105249688 20.258978892740505 94-95 20.20075678441698 30.490922832048124 28.655031430133565 20.65328895340133 96-97 20.166259711715185 30.348126090001905 28.820290400442822 20.66532379784009 98-99 20.46956467879918 30.30354314461498 28.783908236640933 20.442983939944913 100-101 20.62834847106923 30.928991572281276 28.050670688499235 20.391989268150258 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1881.0 1 3765.5 2 4909.0 3 4500.0 4 5558.0 5 7530.0 6 9186.5 7 10505.5 8 12734.5 9 15575.5 10 18539.5 11 21820.5 12 25573.0 13 29564.5 14 33947.5 15 38992.5 16 44713.0 17 51989.0 18 61358.5 19 73576.0 20 89932.5 21 110587.5 22 137647.5 23 174469.5 24 222266.0 25 283513.5 26 357021.0 27 448722.0 28 590074.0 29 738565.0 30 896539.5 31 1078785.0 32 1390691.0 33 1682170.5 34 1849235.5 35 2062145.5 36 2220750.5 37 2229292.5 38 2094306.0 39 1918393.0 40 1836716.5 41 1759048.0 42 1672862.0 43 1661440.5 44 1624248.0 45 1563994.5 46 1500471.5 47 1426154.0 48 1360049.5 49 1281391.5 50 1167710.5 51 1042040.0 52 930430.5 53 836372.0 54 743850.0 55 650816.0 56 556112.5 57 457486.5 58 375631.5 59 298641.5 60 220584.0 61 159243.5 62 113884.5 63 78333.0 64 52160.5 65 36784.5 66 26141.0 67 18440.5 68 13308.5 69 10058.0 70 10242.5 71 12395.5 72 12422.0 73 16042.0 74 16761.0 75 10735.5 76 8050.0 77 8149.0 78 7386.5 79 4771.0 80 2879.5 81 1721.5 82 1286.0 83 1036.0 84 779.0 85 617.5 86 480.0 87 290.5 88 171.5 89 146.5 90 119.0 91 82.0 92 48.5 93 31.0 94 19.0 95 7.0 96 3.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02233349694803916 2 3.584111847227949E-5 3 0.007392230684907645 4 0.16480418294525467 5 0.001209637748439433 6 0.0010617931347412799 7 3.808118837679696E-5 8 0.0 9 0.0 10-11 3.360104856776202E-6 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.7121782565195436E-5 22-23 1.310440894142719E-4 24-25 5.7121782565195436E-5 26-27 6.160192237423037E-5 28-29 7.616237675359392E-4 30-31 2.800087380646835E-5 32-33 7.392230684907645E-5 34-35 6.720209713552404E-4 36-37 2.7440856330338985E-4 38-39 4.323334915718714E-4 40-41 3.416106604389139E-4 42-43 6.160192237423037E-5 44-45 0.0011245150920677692 46-47 0.005614735215673034 48-49 6.944216704004151E-5 50-51 0.0 52-53 7.840244665811138E-6 54-55 1.377642991278243E-4 56-57 2.1392667588141824E-4 58-59 0.001757334840093954 60-61 0.005466890601974882 62-63 0.006683248560127867 64-65 0.016019859922156675 66-67 0.010420805195815263 68-69 4.76014854709962E-4 70-71 3.2593017110729163E-4 72-73 4.368136313809063E-5 74-75 3.729716391021585E-4 76-77 2.9120908758727085E-5 78-79 2.240069904517468E-6 80-81 2.4080751473562787E-4 82-83 8.176255151488759E-5 84-85 1.1648363503490834E-4 86-87 0.0 88-89 1.120034952258734E-6 90-91 4.8161502947125575E-4 92-93 0.005249603821236687 94-95 0.02919819117043294 96-97 0.004943834279270053 98-99 2.6096814387628506E-4 100-101 0.053744877184135356 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.4641464E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.13505955808343 #Duplication Level Percentage of deduplicated Percentage of total 1 72.4490862364585 29.077483910289843 2 11.668917393196953 9.366653891086306 3 5.10869492744179 6.151133255308648 4 2.8570748398873773 4.586754754431262 5 1.7901644068117981 3.59241775430763 6 1.1794236517654881 2.840174310469207 7 0.8529394022161577 2.3962941595167093 8 0.6272521435775202 2.0139841712335413 9 0.4884999287857664 1.7645376362342626 >10 2.7124608737670917 19.533300305789243 >50 0.1689263145602722 4.6230166255162235 >100 0.08555243147136882 6.326757857284796 >500 0.006381872954807927 1.7396564633261506 >1k 0.004003180003964529 3.1168880384628723 >5k 4.0738725755180355E-4 1.1457529163886648 >10k+ 2.1500984362453612E-4 1.7251939503547495 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 46628 0.10444997950784052 No Hit GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA 45690 0.10234879393740312 No Hit CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 45384 0.10166333254662079 No Hit ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 45319 0.10151772800282716 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.03253925543302075 2.240069904517468E-6 0.0 8.960279618069873E-6 0.0 2 0.03321575654418502 1.1200349522587342E-5 0.0 1.7920559236139745E-5 0.0 3 0.03343976353463677 1.1200349522587342E-5 0.0 1.7920559236139745E-5 0.0 4 0.03433803156634827 1.1200349522587342E-5 0.0 2.2400699045174684E-5 0.0 5 0.03456651869660905 1.1200349522587342E-5 0.0 2.912090875872709E-5 0.0 6 0.03506157414550742 1.1200349522587342E-5 0.0 3.136097866324456E-5 0.0 7 0.03526094036700947 1.1200349522587342E-5 0.0 3.584111847227949E-5 0.0 8 0.03559471078278257 1.568048933162228E-5 0.0 4.25613281858319E-5 0.0 9 0.03612784742005773 1.568048933162228E-5 0.0 4.928153789938431E-5 0.0 10-11 0.03756037212399665 1.904059418839848E-5 0.0 5.264164275616051E-5 0.0 12-13 0.039881084545076745 2.352073399743342E-5 0.0 5.936185246971291E-5 0.0 14-15 0.04263301042277646 2.688083885420962E-5 0.0 6.496202723100659E-5 0.0 16-17 0.04600095552421847 2.688083885420962E-5 0.0 7.728241170585265E-5 0.0 18-19 0.04937226073051726 2.912090875872709E-5 0.0 8.064251656262886E-5 0.0 20-21 0.052623722196924363 3.58411184722795E-5 0.0 8.288258646714633E-5 0.0 22-23 0.055763180168105594 3.808118837679696E-5 0.0 8.960279618069874E-5 0.0 24-25 0.05864054996045828 3.808118837679696E-5 0.0 9.85630757987686E-5 0.0 26-27 0.06133871416044957 4.25613281858319E-5 0.0 1.0640332046457974E-4 0.0 28-29 0.06393719524968984 4.704146799486684E-5 0.0 1.176036699871671E-4 0.0 30-31 0.06656255717778431 4.816150294712557E-5 0.0 1.2880401950975443E-4 0.0 32-33 0.06921928008454203 4.928153789938431E-5 0.0 1.444845088413767E-4 0.0 34-35 0.07197680613700302 5.152160780390177E-5 0.0 1.7920559236139744E-4 0.0 36-37 0.07468281058166014 5.152160780390177E-5 0.0 1.9600611664527849E-4 0.0 38-39 0.07777186697998972 5.152160780390177E-5 1.120034952258734E-6 2.1168660597690076E-4 0.0 40-41 0.08121597445818533 5.376167770841924E-5 2.240069904517468E-6 2.2736709530852303E-4 0.0 42-43 0.08469704308980548 5.600174761293671E-5 2.240069904517468E-6 2.6096814387628506E-4 0.0 44-45 0.08832483630017152 5.824181751745418E-5 2.240069904517468E-6 2.82248807969201E-4 0.0 46-47 0.09255072817504373 5.824181751745418E-5 2.240069904517468E-6 2.956892273963058E-4 0.0 48-49 0.09719215301720391 5.824181751745418E-5 2.240069904517468E-6 3.012894021575995E-4 0.0 50-51 0.10204526446534101 5.824181751745418E-5 2.240069904517468E-6 3.337704157731028E-4 0.0 52-53 0.10715038377773631 5.936185246971291E-5 2.240069904517468E-6 3.5169097500924255E-4 0.0 54-55 0.11240110763392527 6.048188742197165E-5 2.240069904517468E-6 3.6849149929312354E-4 0.0 56-57 0.11829137144785395 6.048188742197165E-5 2.240069904517468E-6 3.774517789111934E-4 0.0 58-59 0.12450420532803314 6.608206218326531E-5 2.240069904517468E-6 3.9089219833829826E-4 0.0 60-61 0.1311684132939726 6.944216704004152E-5 2.240069904517468E-6 4.0545265271766176E-4 0.0 62-63 0.13871072866248293 6.944216704004152E-5 2.240069904517468E-6 4.1217286243121415E-4 0.0 64-65 0.14690154426835106 7.280227189681771E-5 2.240069904517468E-6 4.1889307214476655E-4 0.0 66-67 0.15582598276794865 8.288258646714633E-5 2.240069904517468E-6 4.323334915718714E-4 0.0 68-69 0.16624902803366842 8.288258646714633E-5 2.240069904517468E-6 4.368136313809063E-4 0.0 70-71 0.17805307639552323 8.51226563716638E-5 2.240069904517468E-6 4.4801398090349367E-4 2.240069904517468E-6 72-73 0.19176230421117013 8.624269132392252E-5 2.240069904517468E-6 4.5025405080801115E-4 2.240069904517468E-6 74-75 0.20758279791182477 9.072283113295746E-5 2.240069904517468E-6 4.558542255693048E-4 2.240069904517468E-6 76-77 0.2257318442782253 9.296290103747495E-5 2.240069904517468E-6 4.6705457509189213E-4 2.240069904517468E-6 78-79 0.24732499812282144 9.52029709419924E-5 2.240069904517468E-6 4.748948197577033E-4 2.240069904517468E-6 80-81 0.27393142841372764 9.632300589425114E-5 2.240069904517468E-6 4.939354139461018E-4 2.240069904517468E-6 82-83 0.3057695419666344 9.744304084650989E-5 2.240069904517468E-6 5.006556236596541E-4 2.240069904517468E-6 84-85 0.346494012830762 9.856307579876861E-5 2.240069904517468E-6 5.062557984209478E-4 4.480139809034936E-6 86-87 0.39728311777588654 9.856307579876861E-5 2.240069904517468E-6 5.174561479435351E-4 4.480139809034936E-6 88-89 0.45991883241105175 9.968311075102734E-5 2.240069904517468E-6 5.275364625138638E-4 4.480139809034936E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 35775 0.0 45.918648 1 GCGTGTT 40830 0.0 38.291107 2 ATTCGAC 4575 0.0 29.853056 1 CGATAGT 3500 0.0 28.95485 2 CGCGTTT 2315 0.0 28.162262 1 CGTGTTT 55890 0.0 27.453922 3 GTTTTTT 377365 0.0 26.173336 1 CCGATAG 4240 0.0 23.23285 1 CTGGAAT 93350 0.0 22.032736 1 CGTCTCA 6780 0.0 20.424963 1 TTCGACT 8065 0.0 19.93986 2 CGATTCA 5215 0.0 18.341705 1 TTCGTAT 7080 0.0 17.408625 1 CGGGTTT 10325 0.0 17.099438 1 CGGCGAG 3550 0.0 16.756357 1 GCGGGTT 4415 0.0 16.383656 1 TATGGCG 110195 0.0 16.33882 7 CGGGGAT 4275 0.0 16.252295 1 CGGACGT 1935 0.0 16.231586 1 TCGACTC 9030 0.0 16.228252 3 >>END_MODULE