FastQCFastQC Report
Fri 27 May 2016
SRR1233067_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233067_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78401973
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG1043100.13304512119867187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT584700.049.494271
GCGTGTT810250.032.801652
GTTTTTT10080500.031.426211
CGGCGAG42950.029.9597511
CGCGTGC33100.026.5929361
CGCGTAT21900.026.057311
GTCGCCG99350.025.93134544-45
CGCCGTA99400.025.53155546-47
CGCGTTT36050.025.141041
CCGTATC110250.022.98339748-49
TCGCCGT103100.022.50076146-47
CGTGTTT1213650.021.93641
GGTCGCC112650.021.28227844-45
CGTATCA114000.020.67883550-51
TGGTCGC119000.020.61187642-43
CGGACGT23100.019.8598231
CTGGAAT1441550.019.3506741
GTGGTCG130250.018.01654642-43
GGAAAAC2037250.017.4455761
GCCGTAT137600.017.27770848-49
Produced by FastQC (version 0.11.5)