##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233067_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 78401973 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.154167574328774 31.0 31.0 34.0 28.0 34.0 2 31.312013448947262 33.0 31.0 34.0 28.0 34.0 3 31.326272031444923 33.0 31.0 34.0 27.0 34.0 4 34.74849843383406 37.0 35.0 37.0 32.0 37.0 5 34.70966751053574 37.0 35.0 37.0 32.0 37.0 6 34.7893610279425 37.0 35.0 37.0 32.0 37.0 7 34.897339981482354 37.0 35.0 37.0 32.0 37.0 8 34.83075550917577 37.0 35.0 37.0 32.0 37.0 9 36.35483635086581 39.0 37.0 39.0 32.0 39.0 10-11 36.21121125995133 39.0 36.0 39.0 31.5 39.0 12-13 36.15708743707253 39.0 35.0 39.0 31.0 39.0 14-15 37.28860623188654 40.0 37.0 41.0 31.0 41.0 16-17 37.09346083037987 40.0 36.5 41.0 31.0 41.0 18-19 36.91406385908171 39.5 36.0 41.0 30.5 41.0 20-21 36.78121578649558 39.5 36.0 41.0 30.0 41.0 22-23 36.523663983558166 39.0 36.0 41.0 29.5 41.0 24-25 36.20092137859847 39.0 36.0 41.0 27.5 41.0 26-27 36.05003592830502 39.0 36.0 41.0 27.0 41.0 28-29 35.86894371905666 39.0 36.0 41.0 27.0 41.0 30-31 35.701303562858044 39.0 35.5 41.0 26.0 41.0 32-33 35.60327824913284 39.0 35.0 41.0 25.5 41.0 34-35 35.49388837446731 39.0 35.0 41.0 25.0 41.0 36-37 35.35986077799343 39.0 35.0 41.0 25.0 41.0 38-39 35.25957997893752 39.0 35.0 41.0 25.0 41.0 40-41 35.09823463881451 39.0 35.0 41.0 24.0 41.0 42-43 34.92633946214593 39.0 34.5 41.0 24.0 41.0 44-45 34.870399466860356 39.0 34.5 41.0 24.0 41.0 46-47 34.87798671214563 39.0 35.0 41.0 24.0 41.0 48-49 34.803668167891644 39.0 34.0 41.0 23.0 41.0 50-51 34.358387530630125 38.5 33.5 40.5 23.0 41.0 52-53 34.33376475742517 38.5 34.0 40.0 23.0 41.0 54-55 34.50819277851592 39.0 34.0 41.0 22.5 41.0 56-57 34.30510807425726 39.0 34.0 41.0 20.0 41.0 58-59 33.59770257950014 38.5 34.0 40.5 7.5 41.0 60-61 22.744697585607952 19.0 2.0 39.0 2.0 41.0 62-63 17.985977451103174 2.0 2.0 38.0 2.0 41.0 64-65 17.84705963330795 2.0 2.0 38.0 2.0 41.0 66-67 17.688442917118934 2.0 2.0 37.0 2.0 40.0 68-69 17.467660871238536 2.0 2.0 37.0 2.0 40.0 70-71 17.35585312374728 2.0 2.0 36.0 2.0 40.0 72-73 17.184671334737963 2.0 2.0 36.0 2.0 39.0 74-75 16.984495568753097 2.0 2.0 35.5 2.0 39.0 76-77 16.788931778540828 2.0 2.0 35.0 2.0 39.0 78-79 16.575654728740055 2.0 2.0 35.0 2.0 37.5 80-81 16.39733545991247 2.0 2.0 35.0 2.0 37.0 82-83 16.161435031743398 2.0 2.0 35.0 2.0 37.0 84-85 16.00085251043363 2.0 2.0 34.5 2.0 36.0 86-87 15.837569132348237 2.0 2.0 34.0 2.0 36.0 88-89 15.663909452890938 2.0 2.0 34.0 2.0 35.5 90-91 15.491588693820244 2.0 2.0 34.0 2.0 35.0 92-93 15.34519372363244 2.0 2.0 34.0 2.0 35.0 94-95 15.184799418249334 2.0 2.0 34.0 2.0 35.0 96-97 14.998742238285253 2.0 2.0 34.0 2.0 35.0 98-99 14.761177521642217 2.0 2.0 34.0 2.0 35.0 100-101 13.877758707679462 2.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5955674936351762 1101 2 0.6826039121532936 1101 3 0.6702590607548835 1101 4 0.6751560506300507 1101 5 0.6525859809644388 1101 6 0.6498859776470951 1101 7 0.6162602604396525 1101 8 0.6890416062444302 1101 9 0.7837891324712345 1101 10-11 0.8161366556149616 1101 12-13 0.874114746669548 1101 14-15 0.9957103245018359 1101 16-17 0.9676101169527627 1101 18-19 0.9775954977620671 1101 20-21 0.8987083103646327 1101 22-23 0.9081877324323742 1101 24-25 0.9596533261352462 1101 26-27 0.9450075379651466 1101 28-29 0.9473023146385202 1101 30-31 0.9066591094234013 1101 32-33 0.9491101731540965 1101 34-35 0.9287875073197185 1101 36-37 0.9184915379012466 1101 38-39 0.8869766949222466 1101 40-41 0.8247522250276305 1101 42-43 0.834193392371759 1101 44-45 0.8416260699261358 1101 46-47 0.9607807863410542 1101 48-49 0.9298421741326237 1101 50-51 0.9082734204220131 1101 52-53 0.8885272651068945 1101 54-55 0.8820294362416732 1101 56-57 0.8340749330692745 1101 58-59 0.8839641176811099 1101 60-61 -6.5468608947848494 1101 62-63 -15.90542700674333 1101 64-65 -15.766983652435545 1101 66-67 -15.608878518633396 1101 68-69 -15.40122797876732 1101 70-71 -15.289165991188707 1101 72-73 -15.114834590116935 1101 74-75 -14.907993079224479 1101 76-77 -14.715921541386024 1101 78-79 -14.507967538607222 1101 80-81 -14.32954641821215 1101 82-83 -14.092815117939487 1101 84-85 -13.935021802414779 1101 86-87 -13.772016154049021 1101 88-89 -13.601697877711162 1101 90-91 -13.432793201529668 1101 92-93 -13.280645142510823 1101 94-95 -13.119483051930374 1101 96-97 -12.92880672753013 1101 98-99 -12.682908886680854 1101 100-101 -11.79940027748225 1102 1 0.5180444837602067 1102 2 0.6431309017911175 1102 3 0.5943449978467399 1102 4 0.6120891401180515 1102 5 0.625246228971271 1102 6 0.602683525508418 1102 7 0.6071705946591877 1102 8 0.6281571862843691 1102 9 0.6420065685491281 1102 10-11 0.6913191043105087 1102 12-13 0.7622355354794195 1102 14-15 0.8673724275038026 1102 16-17 0.8022003972809415 1102 18-19 0.8187237228662738 1102 20-21 0.7947874456836956 1102 22-23 0.76675760142885 1102 24-25 0.8080094354671274 1102 26-27 0.7691399308441689 1102 28-29 0.7341667214070924 1102 30-31 0.7029210610420051 1102 32-33 0.7732750553133982 1102 34-35 0.8107059471553342 1102 36-37 0.824566432352853 1102 38-39 0.7251925688120977 1102 40-41 0.7185460794910341 1102 42-43 0.7025789724434759 1102 44-45 0.7029446180350902 1102 46-47 0.7922101186368522 1102 48-49 0.771625472721702 1102 50-51 0.6429615380954274 1102 52-53 0.5655409562658988 1102 54-55 0.6748556304966016 1102 56-57 0.6199724387857088 1102 58-59 0.6901385297655054 1102 60-61 -6.7538676274482174 1102 62-63 -15.90542700674333 1102 64-65 -15.766983652435545 1102 66-67 -15.608878518633396 1102 68-69 -15.40122797876732 1102 70-71 -15.289165991188707 1102 72-73 -15.114834590116935 1102 74-75 -14.907993079224479 1102 76-77 -14.715921541386024 1102 78-79 -14.507967538607222 1102 80-81 -14.32954641821215 1102 82-83 -14.092815117939487 1102 84-85 -13.935021802414779 1102 86-87 -13.772016154049021 1102 88-89 -13.601697877711162 1102 90-91 -13.432793201529668 1102 92-93 -13.280645142510823 1102 94-95 -13.119483051930374 1102 96-97 -12.92880672753013 1102 98-99 -12.682908886680854 1102 100-101 -11.79940027748225 1103 1 0.46903521196464126 1103 2 0.5978003264203551 1103 3 0.5380709381094526 1103 4 0.5515908888239736 1103 5 0.5929538035502446 1103 6 0.5189423395611641 1103 7 0.5593125661416565 1103 8 0.5701272554211201 1103 9 0.666211307762616 1103 10-11 0.6933081993061236 1103 12-13 0.6844412446127919 1103 14-15 0.7988097410246908 1103 16-17 0.8132384918716511 1103 18-19 0.759797384862587 1103 20-21 0.7052592767069896 1103 22-23 0.7278171799608799 1103 24-25 0.8170733474884173 1103 26-27 0.7271743629524252 1103 28-29 0.7688600645894752 1103 30-31 0.7630647394462997 1103 32-33 0.7910000214434731 1103 34-35 0.7549356372025713 1103 36-37 0.7533030541045349 1103 38-39 0.7702416382256345 1103 40-41 0.7163659849383706 1103 42-43 0.7501290736056276 1103 44-45 0.7104319790092575 1103 46-47 0.7383930015735274 1103 48-49 0.7464169551247437 1103 50-51 0.732662312766827 1103 52-53 0.7979869657146921 1103 54-55 0.7856383331633694 1103 56-57 0.7052151360848384 1103 58-59 0.6772871480026481 1103 60-61 -6.684998330567737 1103 62-63 -15.90542700674333 1103 64-65 -15.766983652435545 1103 66-67 -15.608878518633396 1103 68-69 -15.40122797876732 1103 70-71 -15.289165991188707 1103 72-73 -15.114834590116935 1103 74-75 -14.907993079224479 1103 76-77 -14.715921541386024 1103 78-79 -14.507967538607222 1103 80-81 -14.32954641821215 1103 82-83 -14.092815117939487 1103 84-85 -13.935021802414779 1103 86-87 -13.772016154049021 1103 88-89 -13.601697877711162 1103 90-91 -13.432793201529668 1103 92-93 -13.280645142510823 1103 94-95 -13.119483051930374 1103 96-97 -12.92880672753013 1103 98-99 -12.682908886680854 1103 100-101 -11.79940027748225 1104 1 0.4491590275720405 1104 2 0.5066315875801877 1104 3 0.5100722002115567 1104 4 0.4769782218712635 1104 5 0.5428274309630439 1104 6 0.545537302551061 1104 7 0.5579236863797519 1104 8 0.5667501710984482 1104 9 0.6369615841718499 1104 10-11 0.679491141998291 1104 12-13 0.675763538989294 1104 14-15 0.7943467261527601 1104 16-17 0.7983223498368801 1104 18-19 0.7456436066226004 1104 20-21 0.6545658087550876 1104 22-23 0.7066148474264082 1104 24-25 0.7290603030899234 1104 26-27 0.6431832324386519 1104 28-29 0.6488813974032581 1104 30-31 0.6907436519794317 1104 32-33 0.6526443368461159 1104 34-35 0.6925663062138838 1104 36-37 0.6917671918891131 1104 38-39 0.6301216708955479 1104 40-41 0.5873893974874775 1104 42-43 0.6289123781626884 1104 44-45 0.6273426916818607 1104 46-47 0.6840753588903894 1104 48-49 0.649836579626367 1104 50-51 0.5839639766838758 1104 52-53 0.6494546155440091 1104 54-55 0.6698504017645064 1104 56-57 0.5679795905354013 1104 58-59 0.6001440472479089 1104 60-61 -5.880712303148641 1104 62-63 -15.90542700674333 1104 64-65 -15.766983652435545 1104 66-67 -15.608878518633396 1104 68-69 -15.40122797876732 1104 70-71 -15.289165991188707 1104 72-73 -15.114834590116935 1104 74-75 -14.907993079224479 1104 76-77 -14.715921541386024 1104 78-79 -14.507967538607222 1104 80-81 -14.32954641821215 1104 82-83 -14.092815117939487 1104 84-85 -13.935021802414779 1104 86-87 -13.772016154049021 1104 88-89 -13.601697877711162 1104 90-91 -13.432793201529668 1104 92-93 -13.280645142510823 1104 94-95 -13.119483051930374 1104 96-97 -12.92880672753013 1104 98-99 -12.682908886680854 1104 100-101 -11.79940027748225 1105 1 0.5414691856062319 1105 2 0.6518887294318354 1105 3 0.5904985015425694 1105 4 0.6137648416248709 1105 5 0.6511360200242322 1105 6 0.6097775086040684 1105 7 0.5840198533725456 1105 8 0.6245257481821866 1105 9 0.7355273313881057 1105 10-11 0.7724647954867407 1105 12-13 0.7688079834199257 1105 14-15 0.8877048989364766 1105 16-17 0.9155966645425337 1105 18-19 0.7840293249985422 1105 20-21 0.8043943001266669 1105 22-23 0.8721316856119259 1105 24-25 0.9339998466034558 1105 26-27 0.8569776567276151 1105 28-29 0.9165825237792689 1105 30-31 0.8993230865649693 1105 32-33 0.9047345161086966 1105 34-35 0.8877295679493074 1105 36-37 0.9292712328251298 1105 38-39 0.8794642989899515 1105 40-41 0.9010427188595784 1105 42-43 0.9047556147344409 1105 44-45 0.9057456901264302 1105 46-47 0.9612355612100814 1105 48-49 0.9236423789373731 1105 50-51 0.897117738563125 1105 52-53 0.8882098422552005 1105 54-55 0.9039323188804147 1105 56-57 0.8287057098645292 1105 58-59 0.9837720124315226 1105 60-61 -5.6904738509705055 1105 62-63 -15.90542700674333 1105 64-65 -15.766983652435545 1105 66-67 -15.608878518633396 1105 68-69 -15.40122797876732 1105 70-71 -15.289165991188707 1105 72-73 -15.114834590116935 1105 74-75 -14.907993079224479 1105 76-77 -14.715921541386024 1105 78-79 -14.507967538607222 1105 80-81 -14.32954641821215 1105 82-83 -14.092815117939487 1105 84-85 -13.935021802414779 1105 86-87 -13.772016154049021 1105 88-89 -13.601697877711162 1105 90-91 -13.432793201529668 1105 92-93 -13.280645142510823 1105 94-95 -13.119483051930374 1105 96-97 -12.92880672753013 1105 98-99 -12.682908886680854 1105 100-101 -11.79940027748225 1106 1 0.2570437009698239 1106 2 0.4281544752755373 1106 3 0.3823364558239959 1106 4 0.39399324693661697 1106 5 0.4159692281575573 1106 6 0.41995199547238116 1106 7 0.3764264192675171 1106 8 0.40700516985666724 1106 9 0.4507066706459284 1106 10-11 0.4536528931250743 1106 12-13 0.5322721005042226 1106 14-15 0.6496897814611202 1106 16-17 0.5427884105941416 1106 18-19 0.5393744280682142 1106 20-21 0.5295572700887377 1106 22-23 0.5502500413596181 1106 24-25 0.5710229652668133 1106 26-27 0.5593325721056601 1106 28-29 0.5150884085790253 1106 30-31 0.5125712354887995 1106 32-33 0.5201384792770298 1106 34-35 0.6002584079953692 1106 36-37 0.5798970361357192 1106 38-39 0.5132602189593456 1106 40-41 0.46055443242789096 1106 42-43 0.508729405439972 1106 44-45 0.5229712964708 1106 46-47 0.5738521592776209 1106 48-49 0.4808143628407038 1106 50-51 0.42559328114356987 1106 52-53 0.45152009621767775 1106 54-55 0.4299619209288821 1106 56-57 0.29141048402432546 1106 58-59 0.37532673834728314 1106 60-61 -6.7560237592014545 1106 62-63 -15.90542700674333 1106 64-65 -15.766983652435545 1106 66-67 -15.608878518633396 1106 68-69 -15.40122797876732 1106 70-71 -15.289165991188707 1106 72-73 -15.114834590116935 1106 74-75 -14.907993079224479 1106 76-77 -14.715921541386024 1106 78-79 -14.507967538607222 1106 80-81 -14.32954641821215 1106 82-83 -14.092815117939487 1106 84-85 -13.935021802414779 1106 86-87 -13.772016154049021 1106 88-89 -13.601697877711162 1106 90-91 -13.432793201529668 1106 92-93 -13.280645142510823 1106 94-95 -13.119483051930374 1106 96-97 -12.92880672753013 1106 98-99 -12.682908886680854 1106 100-101 -11.79940027748225 1107 1 0.16933495535146648 1107 2 0.21669607694808235 1107 3 0.21855101969991608 1107 4 0.2697549879807042 1107 5 0.277358701646115 1107 6 0.30596415246291997 1107 7 0.25731525207973505 1107 8 0.3454567246785132 1107 9 0.3208337153306502 1107 10-11 0.38286647632688897 1107 12-13 0.3978030490168507 1107 14-15 0.401236052219339 1107 16-17 0.3901029651422334 1107 18-19 0.37696267515472925 1107 20-21 0.347007025050722 1107 22-23 0.3084106475418622 1107 24-25 0.34208997551331066 1107 26-27 0.28957949559818275 1107 28-29 0.2972891050591002 1107 30-31 0.23877182093957572 1107 32-33 0.2763342819421908 1107 34-35 0.28690904408353646 1107 36-37 0.3015511088407976 1107 38-39 0.3015505224841988 1107 40-41 0.2789427139134588 1107 42-43 0.24009101822377943 1107 44-45 0.2255478752068285 1107 46-47 0.2688676586815575 1107 48-49 0.21319496727008413 1107 50-51 0.1783598342166144 1107 52-53 0.23241665861035443 1107 54-55 0.22013984971089684 1107 56-57 0.17288147681570365 1107 58-59 0.17056936316281934 1107 60-61 -6.992063662968789 1107 62-63 -15.90542700674333 1107 64-65 -15.766983652435545 1107 66-67 -15.608878518633396 1107 68-69 -15.40122797876732 1107 70-71 -15.289165991188707 1107 72-73 -15.114834590116935 1107 74-75 -14.907993079224479 1107 76-77 -14.715921541386024 1107 78-79 -14.507967538607222 1107 80-81 -14.32954641821215 1107 82-83 -14.092815117939487 1107 84-85 -13.935021802414779 1107 86-87 -13.772016154049021 1107 88-89 -13.601697877711162 1107 90-91 -13.432793201529668 1107 92-93 -13.280645142510823 1107 94-95 -13.119483051930374 1107 96-97 -12.92880672753013 1107 98-99 -12.682908886680854 1107 100-101 -11.79940027748225 1108 1 0.015627643953141757 1108 2 0.1480886410101938 1108 3 0.128030185659199 1108 4 0.18975919998920432 1108 5 0.24697719089181902 1108 6 0.2107508222431207 1108 7 0.172061956224546 1108 8 0.2616709467683975 1108 9 0.23760244729617597 1108 10-11 0.282570456088159 1108 12-13 0.2473134748421515 1108 14-15 0.2532990727023119 1108 16-17 0.2402584335635609 1108 18-19 0.2012781191177737 1108 20-21 0.2017843279252105 1108 22-23 0.14651277465887347 1108 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-0.08709425757221823 2312 58-59 0.4064793625382137 2312 60-61 10.995058720620484 2312 62-63 15.391689355805276 2312 64-65 15.239579314959013 2312 66-67 15.146819955790775 2312 68-69 15.09174838976394 2312 70-71 14.878461887961752 2312 72-73 14.647654192413725 2312 74-75 14.491289002737833 2312 76-77 14.280040169651965 2312 78-79 14.09284011918518 2312 80-81 13.913546612000236 2312 82-83 13.722799599380288 2312 84-85 13.451930037250195 2312 86-87 13.238124438233362 2312 88-89 13.054090336467722 2312 90-91 12.90047631686698 2312 92-93 12.814790095299527 2312 94-95 12.6587221529754 2312 96-97 12.322189383747165 2312 98-99 11.93660053599339 2312 100-101 11.055999064426215 2313 1 -0.2514672552072277 2313 2 -0.3000760251277619 2313 3 -0.27168877636899325 2313 4 -0.21600805599252482 2313 5 -0.22199128194411344 2313 6 -0.39947711648665774 2313 7 -0.23707745362973043 2313 8 -0.36830400520227613 2313 9 -0.31920845487996985 2313 10-11 -0.24923332816919697 2313 12-13 -0.2853298738021621 2313 14-15 -0.4140730136456767 2313 16-17 -0.5038063944984685 2313 18-19 -0.5696936426318686 2313 20-21 -0.5990234319976224 2313 22-23 -0.5644309008522299 2313 24-25 -0.4391159469628718 2313 26-27 -0.4151583034170656 2313 28-29 -0.3804976307984447 2313 30-31 -0.5389462053319036 2313 32-33 -0.44961585976933094 2313 34-35 -0.4822442042234272 2313 36-37 -0.43399151737634156 2313 38-39 -0.37960719041500823 2313 40-41 -0.5748749685952887 2313 42-43 -0.4538465735103898 2313 44-45 -0.5342659277794155 2313 46-47 -0.4843447629979565 2313 48-49 -0.486931333665261 2313 50-51 -0.4105464218990207 2313 52-53 -0.4799065397260378 2313 54-55 -0.4163518786446829 2313 56-57 -0.20315358014938312 2313 58-59 0.20807280251591465 2313 60-61 10.769663416207742 2313 62-63 15.411223588328617 2313 64-65 15.316021904952738 2313 66-67 15.055070779714612 2313 68-69 14.965166316662422 2313 70-71 14.853384824656192 2313 72-73 14.682917078368359 2313 74-75 14.43782111622303 2313 76-77 14.237259365559446 2313 78-79 13.999943492427871 2313 80-81 13.80440820913832 2313 82-83 13.621195083293795 2313 84-85 13.411896257828326 2313 86-87 13.088814409048906 2313 88-89 12.92790980620286 2313 90-91 12.747047943444572 2313 92-93 12.614475013806581 2313 94-95 12.513221797461867 2313 96-97 12.26783346329891 2313 98-99 11.898015822127226 2313 100-101 10.723596905759306 2314 1 -0.5773576713708692 2314 2 -1.1666833834592722 2314 3 -0.780634261807915 2314 4 -0.5047903321533909 2314 5 -0.6285235559252769 2314 6 -0.46917369446638446 2314 7 -0.3654090847746758 2314 8 -1.1769880371779209 2314 9 -2.1553838811832264 2314 10-11 -1.899198081525661 2314 12-13 -1.4356928281114065 2314 14-15 -1.81603064765617 2314 16-17 -1.4078141825626247 2314 18-19 -1.0819344096016437 2314 20-21 -1.013507572068157 2314 22-23 -0.7561575988031279 2314 24-25 -0.764308038033704 2314 26-27 -0.9802425892257602 2314 28-29 -0.9049464553153257 2314 30-31 -0.8590998973207107 2314 32-33 -0.9099533584601645 2314 34-35 -0.7937768248717916 2314 36-37 -0.7982240988162772 2314 38-39 -0.7716448949418435 2314 40-41 -0.8358710747180425 2314 42-43 -0.7388045878640952 2314 44-45 -0.7381520892295157 2314 46-47 -0.7606891383022756 2314 48-49 -0.7009549588867756 2314 50-51 -0.6242890617290371 2314 52-53 -0.7231377787769802 2314 54-55 -0.6989189242859624 2314 56-57 -0.5729682864914807 2314 58-59 -0.21878739347977216 2314 60-61 10.42665569383368 2314 62-63 15.06589468901727 2314 64-65 14.807992741530992 2314 66-67 14.725764243393577 2314 68-69 14.668597457641656 2314 70-71 14.371088711859496 2314 72-73 14.088976271079588 2314 74-75 13.931994330891008 2314 76-77 13.79691650911878 2314 78-79 13.67489448110079 2314 80-81 13.526535464459812 2314 82-83 13.274803456982433 2314 84-85 13.051643815094844 2314 86-87 12.818219422059252 2314 88-89 12.692154869062682 2314 90-91 12.419106176239504 2314 92-93 12.275186153036719 2314 94-95 12.18421764777667 2314 96-97 11.945252057546014 2314 98-99 11.485238705503734 2314 100-101 10.437247181540096 2315 1 -1.2174484259511402 2315 2 -2.6848162001980107 2315 3 -1.4463353714791012 2315 4 -2.043622209755995 2315 5 -1.6695418399280797 2315 6 -0.9768521834117792 2315 7 -0.8005406011716119 2315 8 -0.8565542878744736 2315 9 -1.0689904528584222 2315 10-11 -1.4428669473032372 2315 12-13 -1.7181275808616263 2315 14-15 -3.3857072277938443 2315 16-17 -2.8846949005876965 2315 18-19 -1.8994382247339274 2315 20-21 -2.999186162365376 2315 22-23 -3.914651125483502 2315 24-25 -2.523769510398921 2315 26-27 -1.6599524464296351 2315 28-29 -2.6883783437303777 2315 30-31 -1.927007048716618 2315 32-33 -1.9606926607789958 2315 34-35 -3.6542624908060333 2315 36-37 -3.9597377396458953 2315 38-39 -3.210047127541337 2315 40-41 -1.8394632616420452 2315 42-43 -2.5050550135226075 2315 44-45 -2.8573562885817516 2315 46-47 -1.886517707488828 2315 48-49 -1.4989729461088146 2315 50-51 -2.262552894532874 2315 52-53 -1.5689749901003367 2315 54-55 -1.4214328379611558 2315 56-57 -1.451902891162824 2315 58-59 -1.450321607888739 2315 60-61 9.689990651545159 2315 62-63 14.443806096239982 2315 64-65 14.408854877818964 2315 66-67 14.204741628565884 2315 68-69 14.201474420243859 2315 70-71 13.671182904816686 2315 72-73 13.4999471391937 2315 74-75 13.605062429511964 2315 76-77 13.477031714414863 2315 78-79 13.02292605092028 2315 80-81 12.798529597294337 2315 82-83 12.687966105724762 2315 84-85 12.415938925716015 2315 86-87 12.242507133776138 2315 88-89 12.034370159819035 2315 90-91 11.905638732848667 2315 92-93 11.722676673321528 2315 94-95 11.260784097909507 2315 96-97 11.009574642209742 2315 98-99 10.399356970951596 2315 100-101 9.471338247153964 2316 1 -3.446201785357289 2316 2 -2.497705498919057 2316 3 -3.824204007550332 2316 4 -2.6044569577927064 2316 5 -2.8330303683866838 2316 6 -1.881117084586819 2316 7 -1.7845116175400548 2316 8 -2.395216445908673 2316 9 -2.5683686053992147 2316 10-11 -4.80344708020634 2316 12-13 -4.6398314000946925 2316 14-15 -4.699433444232071 2316 16-17 -5.09969971691369 2316 18-19 -4.050712296391183 2316 20-21 -3.8992714974493694 2316 22-23 -3.9098508211136718 2316 24-25 -3.1282241300359317 2316 26-27 -4.12718744863291 2316 28-29 -3.913259730319538 2316 30-31 -4.385536034364868 2316 32-33 -4.264617179838012 2316 34-35 -4.799836656236884 2316 36-37 -3.8244782316918986 2316 38-39 -3.9894984293125475 2316 40-41 -4.196145919904282 2316 42-43 -4.593579326368026 2316 44-45 -4.483118795039189 2316 46-47 -5.704390895967556 2316 48-49 -5.38893706466434 2316 50-51 -4.329724882232355 2316 52-53 -4.217752459836426 2316 54-55 -4.894030410411812 2316 56-57 -4.607105635564508 2316 58-59 -3.981580044333654 2316 60-61 7.417402321720658 2316 62-63 11.670056435866016 2316 64-65 11.337464849514824 2316 66-67 10.92088882028823 2316 68-69 11.004109946924991 2316 70-71 11.104331394486454 2316 72-73 11.182569067171357 2316 74-75 11.335235957763377 2316 76-77 10.98256657657674 2316 78-79 10.387743032117871 2316 80-81 10.317443650119937 2316 82-83 9.708945747415868 2316 84-85 9.45148330174324 2316 86-87 10.304055165839214 2316 88-89 10.119254068508441 2316 90-91 8.877626750333556 2316 92-93 9.137881982838445 2316 94-95 8.872355828197993 2316 96-97 9.116314582675418 2316 98-99 9.052352684127237 2316 100-101 7.952349521947854 >>END_MODULE >>Per sequence quality scores warn #Quality Count 2 381605.0 3 63255.0 4 71994.0 5 107489.0 6 193923.0 7 330486.0 8 493042.0 9 692794.0 10 743279.0 11 631883.0 12 535717.0 13 510430.0 14 554176.0 15 654887.0 16 817861.0 17 1032697.0 18 1343290.0 19 1801909.0 20 2502303.0 21 3625513.0 22 5567733.0 23 1.0902753E7 24 1.0148228E7 25 410164.0 26 475885.0 27 557838.0 28 659516.0 29 784049.0 30 938035.0 31 1133882.0 32 1386889.0 33 1725353.0 34 2206743.0 35 2923461.0 36 4136375.0 37 6512246.0 38 8331057.0 39 2508026.0 40 5207.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.73980275405361 13.401817520689367 13.9534068268733 37.904972898383726 2 17.52450904844506 18.890763846574156 50.057564375652774 13.527162729328005 3 21.301021677062504 21.70639937647223 37.32346377685881 19.669115169606453 4 21.457682683058987 29.61213801800508 33.38016327466702 15.550016024268917 5 20.569788370862252 30.289923874945323 34.49575543789708 14.644532316295344 6 16.290771628093847 32.129612164432544 36.10078461068403 15.47883159678958 7 13.87659005765347 15.80799925040023 52.500844187925075 17.81456650402122 8 15.776319950340447 20.906075700808657 42.8941290548696 20.423475293981298 9 18.627991859706793 19.866059009776595 41.03699845699969 20.468950673516918 10-11 20.459559448678803 30.956303927754625 31.61133355271834 16.972803070848233 12-13 18.077297248474146 25.351724477815623 38.896244526998174 17.674733746712057 14-15 18.750543433339722 25.395123247901573 38.2657495784785 17.588583740280203 16-17 19.579162493088848 25.919442570465645 35.852798305476064 18.648596630969447 18-19 20.522944820071743 26.286390409007787 34.30025205597633 18.89041271494414 20-21 19.76796984958651 27.099498693278345 34.082632549395775 19.049898907739365 22-23 19.8998985722686 27.451013651216886 34.986858349490475 17.662229427024037 24-25 20.06012165877488 27.493905059138996 34.039830258622736 18.406143023463382 26-27 20.432647092510514 27.989422852430245 32.723974098121026 18.85395595693821 28-29 20.886277458612494 28.289214609314634 31.292167309418716 19.53234062265416 30-31 20.120177539706084 28.12238757064901 32.14456722500602 19.612867664638888 32-33 20.70170128396289 28.11976763278639 31.83921498503774 19.339316098212976 34-35 19.618797051628466 29.098351702044223 31.041558761500742 20.241292484826566 36-37 19.825850598453734 28.41973614405941 31.409340446798673 20.345072810688187 38-39 20.479648363512425 27.620355866574126 31.93351888631406 19.966476883599395 40-41 21.04244974234721 28.02579433530834 31.04411440906274 19.88764151328171 42-43 20.934536817949486 28.90789550741032 30.81820587893492 19.33936179570528 44-45 20.642869010329534 28.739866187915332 30.735734573579958 19.881530228175173 46-47 20.47440441516709 28.58176535746845 30.64335749645291 20.300472730911558 48-49 21.137030159668722 28.522196968048654 29.8062172169266 20.53455565535603 50-51 20.731062673186692 28.726062292131395 30.306596338799224 20.236278695882685 52-53 20.479114431489045 29.045177414622326 30.70014930565701 19.77555884823162 54-55 20.306734905037267 28.985232669926088 30.695341523532438 20.012690901504207 56-57 21.09688796763922 28.226172245961962 30.1298528561538 20.547086930245023 58-59 20.757695862912723 28.748431990864542 29.567648815959686 20.92622333026305 60-61 23.305688821634515 28.920199786678037 27.81144786590815 19.9626635257793 62-63 21.8887998926489 30.2251137702503 28.001507962653356 19.884578374447443 64-65 21.576367199620645 29.876164114642823 28.352522266157383 20.194946419579153 66-67 21.469463167210773 28.732985932005526 29.313810902066745 20.483739998716956 68-69 22.293703695807952 29.5685164104245 27.925486216704705 20.212293677062842 70-71 22.276519529944625 30.28245337131882 27.395293858673163 20.04573324006339 72-73 23.678490631363385 30.116020886108952 26.213829032965403 19.991659449562263 74-75 23.162516726438522 29.330482490995685 27.196338087288918 20.31066269527687 76-77 22.254417163588588 29.24361924330185 27.914584237181828 20.58737935592774 78-79 23.98203898982991 29.959485025557225 25.73008562079364 20.328390363819228 80-81 21.97740460431009 29.826278236234515 28.48560485883699 19.710712300618404 82-83 20.910004281157697 29.988646273389 28.990191897781724 20.111157547671578 84-85 22.48823797091512 30.143218466672245 27.21286414743207 20.15567941498057 86-87 22.768220413450848 30.299756924296204 26.607520842994308 20.324501819258632 88-89 22.383566662943068 30.362293566059606 27.109667095707234 20.14447267529009 90-91 22.169465409179825 30.469474599220515 27.1106282649955 20.25043172660416 92-93 21.116290361661402 30.777863906669374 27.65221907933849 20.453626652330737 94-95 22.196039239948913 30.85183023275913 26.308623624907572 20.643506902384384 96-97 22.481620721432932 30.138915585217347 26.894887251191534 20.484576442158183 98-99 21.464143988350486 30.612418137066477 27.583108490340415 20.34032938424262 100-101 21.42063607410491 31.249149919727813 26.95824094890537 20.3719730572619 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 68630.0 1 53033.5 2 34923.0 3 34826.0 4 41497.0 5 51026.0 6 63033.0 7 78827.5 8 99042.5 9 123781.5 10 155367.0 11 196804.0 12 251161.5 13 320673.5 14 408480.5 15 518280.5 16 649979.5 17 808857.0 18 1006166.5 19 1255084.0 20 1553988.0 21 1873212.0 22 2176615.0 23 2424289.0 24 2566237.5 25 2615940.0 26 2624325.5 27 2622666.0 28 2627182.0 29 2641863.0 30 2680950.0 31 2730732.5 32 2800804.5 33 2875721.5 34 2936988.5 35 2975281.5 36 2936700.5 37 2761059.0 38 2458754.0 39 2143494.0 40 1905774.0 41 1724587.0 42 1582753.5 43 1489521.0 44 1414089.5 45 1353897.0 46 1303487.5 47 1239672.0 48 1172321.5 49 1106542.5 50 1026330.0 51 937387.0 52 844553.5 53 757110.5 54 671266.5 55 583996.5 56 496691.0 57 408200.5 58 325069.0 59 248150.0 60 179914.0 61 126990.5 62 89659.0 63 62423.5 64 42851.5 65 29114.0 66 19730.5 67 13417.5 68 9361.0 69 6613.0 70 4554.0 71 3165.5 72 2324.5 73 1911.0 74 1521.0 75 1081.5 76 819.5 77 649.5 78 542.0 79 428.0 80 312.5 81 247.5 82 217.5 83 157.0 84 114.5 85 104.0 86 91.0 87 69.0 88 50.0 89 36.0 90 27.0 91 21.5 92 18.5 93 18.5 94 15.5 95 12.0 96 9.0 97 8.0 98 5.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.3318015989214965 2 0.27387958718844996 3 0.2508036373013215 4 0.2494796910276735 5 0.24536882509321542 6 0.19950518337083176 7 0.19414817532716938 8 0.20462622796495183 9 0.2060509370089449 10-11 0.19893632013571905 12-13 0.20459179005609973 14-15 0.20535962787569134 16-17 0.21649327625976963 18-19 0.2230874980658969 20-21 0.2079616032111845 22-23 0.17774170045440055 24-25 0.2114423829614594 26-27 0.15956677518817033 28-29 0.15616316186328627 30-31 0.1735932079158263 32-33 0.18886769596984504 34-35 0.19551357463925048 36-37 0.19933618252183527 38-39 0.19512646703419056 40-41 0.17864473895318936 42-43 0.17356514739750234 44-45 0.16939497173113233 46-47 0.17223673695048464 48-49 0.19665385206568717 50-51 0.20246429257590237 52-53 0.2298685774144995 54-55 0.23133027532355596 56-57 0.24030836570911296 58-59 0.21861949826186133 60-61 26.66367337464837 62-63 45.516789481815714 64-65 46.15087046342571 66-67 47.40196589185326 68-69 47.55940580730028 70-71 45.58029553669523 72-73 39.848507383863925 74-75 42.40539201736671 76-77 47.196021457266134 78-79 40.741467054662 80-81 45.96002003674066 82-83 48.6639717599964 84-85 45.42321084190063 86-87 45.16889900206976 88-89 44.83409939186097 90-91 45.79589023862958 92-93 47.641325939590836 94-95 44.21433756520388 96-97 44.93004276818391 98-99 48.52239305253198 100-101 40.90686531574914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7.8401973E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.03712179770192 #Duplication Level Percentage of deduplicated Percentage of total 1 75.21036901330226 32.368378116755956 2 10.439621660367258 8.985825378383058 3 4.4617245718488565 5.7605935147937615 4 2.479252176021983 4.267995114667024 5 1.5742070188428652 3.387466960236882 6 1.094365591555558 2.8259007152994395 7 0.7700993236231959 2.320000087096955 8 0.571728516693761 1.968443984653508 9 0.4578629705833887 1.7734593988489418 >10 2.695301909813557 21.01946280986465 >50 0.1666768744620322 4.896759896110619 >100 0.07206453161567504 5.4371696649776755 >500 0.004116312970847036 1.2013764629839145 >1k 0.0022822545868042385 1.8397849788255274 >5k 2.1126494115883626E-4 0.6114342996829537 >10k+ 1.1600877083565264E-4 1.3359486168192822 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG 104310 0.13304512119867187 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.07852863600766782 1.2754781056338979E-6 0.0 8.928346739437284E-6 0.0 2 0.07920719035986505 3.8264343169016936E-6 0.0 3.061147453521355E-5 0.0 3 0.07927734165567492 3.8264343169016936E-6 0.0 3.316243074648134E-5 6.377390528169488E-6 4 0.07968421917137213 3.8264343169016936E-6 0.0 4.209077748591863E-5 6.377390528169488E-6 5 0.07980666506951298 3.8264343169016936E-6 0.0 4.974364611972201E-5 6.377390528169488E-6 6 0.079987782960513 3.8264343169016936E-6 0.0 6.249842717606099E-5 6.377390528169488E-6 7 0.08009364764328061 3.8264343169016936E-6 1.2754781056338979E-6 6.760033959859658E-5 6.377390528169488E-6 8 0.08079516060137926 5.101912422535591E-6 1.2754781056338979E-6 8.290607686620335E-5 6.377390528169488E-6 9 0.08099923709828068 5.101912422535591E-6 1.2754781056338979E-6 9.183442360564064E-5 6.377390528169488E-6 10-11 0.08166312345226312 5.101912422535591E-6 1.2754781056338979E-6 1.0012503129226097E-4 6.377390528169488E-6 12-13 0.08280084992248855 6.377390528169488E-6 1.2754781056338979E-6 1.1160433424296605E-4 7.652868633803387E-6 14-15 0.08411395463223865 7.652868633803387E-6 1.2754781056338979E-6 1.2627233245775588E-4 8.928346739437286E-6 16-17 0.08533650039648874 8.290607686620336E-6 1.2754781056338979E-6 1.5433285078170163E-4 1.0203824845071183E-5 18-19 0.08678671900259449 8.928346739437284E-6 1.2754781056338979E-6 1.6772537089085755E-4 1.0203824845071183E-5 20-21 0.08832367011988333 8.928346739437284E-6 1.2754781056338979E-6 1.7665371763029485E-4 1.0203824845071183E-5 22-23 0.08972350734081654 8.928346739437284E-6 1.2754781056338979E-6 2.0088780163733888E-4 1.4667998214789826E-5 24-25 0.09096964945002087 8.928346739437284E-6 1.2754781056338979E-6 2.270351028028338E-4 4.017756032746778E-5 26-27 0.09221515382017235 9.566085792254234E-6 2.5509562112677957E-6 2.4808049154579313E-4 4.081529938028473E-5 28-29 0.09349573383822879 1.147930295070508E-5 2.5509562112677957E-6 2.74865531764105E-4 4.272851653873557E-5 30-31 0.09477248742196832 1.2117042003522028E-5 2.5509562112677957E-6 2.927222252429795E-4 4.400399464436948E-5 32-33 0.09621951733280998 1.2754781056338977E-5 2.5509562112677957E-6 3.080279625105863E-4 4.974364611972201E-5 34-35 0.09790378617129955 1.3392520109155925E-5 2.5509562112677957E-6 3.7626604116199985E-4 5.0381385172538955E-5 36-37 0.09942606929037359 1.4030259161972876E-5 3.1886952640847446E-6 4.330248168627083E-4 5.420781948944065E-5 38-39 0.10116709690456388 1.4030259161972876E-5 3.8264343169016936E-6 4.8340620203524723E-4 5.8671992859159296E-5 40-41 0.10319829578778586 1.5943476320423723E-5 3.8264343169016936E-6 5.172063718345456E-4 6.0585210017610145E-5 42-43 0.10546163168623321 1.913217158450847E-5 3.8264343169016936E-6 5.4973106352821E-4 6.504938338732878E-5 44-45 0.10812801866606087 2.1683127795776263E-5 3.8264343169016936E-6 5.950105362782132E-4 7.078903486268132E-5 46-47 0.11089070424286389 2.295860590141016E-5 3.8264343169016936E-6 6.581467025070912E-4 7.461546917958302E-5 48-49 0.11393654595911763 2.295860590141016E-5 3.8264343169016936E-6 6.887581770423048E-4 8.290607686620335E-5 50-51 0.1160946549138502 2.4871823059861005E-5 3.8264343169016936E-6 7.225583468416031E-4 8.290607686620335E-5 52-53 0.11815965396687148 2.5509562112677954E-5 3.8264343169016936E-6 7.365886060035759E-4 8.290607686620335E-5 54-55 0.12045232586174841 2.5509562112677954E-5 3.8264343169016936E-6 7.506188651655488E-4 8.290607686620335E-5 56-57 0.12280175653232604 2.5509562112677954E-5 3.8264343169016936E-6 7.723019929613251E-4 8.290607686620335E-5 58-59 0.12546559255594245 2.5509562112677954E-5 3.8264343169016936E-6 7.99087033179637E-4 8.60947721302881E-5 60-61 0.12847699636334406 2.5509562112677954E-5 3.8264343169016936E-6 8.277852905563996E-4 9.566085792254232E-5 62-63 0.1319137721189746 2.5509562112677954E-5 3.8264343169016936E-6 8.564835479331623E-4 1.0395146560916266E-4 64-65 0.13587285615886224 2.5509562112677954E-5 3.8264343169016936E-6 8.737025023592199E-4 1.0714016087324742E-4 66-67 0.14065845001120061 2.6785040218311854E-5 3.8264343169016936E-6 8.909214567852776E-4 1.0777789992606437E-4 68-69 0.14656008720596866 2.6785040218311854E-5 3.8264343169016936E-6 8.9666110826063E-4 1.084156389788813E-4 70-71 0.15385199553587764 2.7422779271128803E-5 3.8264343169016936E-6 9.419405810106335E-4 1.0969111708451521E-4 72-73 0.16289003339239944 2.806051832394575E-5 3.8264343169016936E-6 9.827558803909182E-4 1.147930295070508E-4 74-75 0.17415569376041085 2.806051832394575E-5 3.8264343169016936E-6 0.0010140050939789487 1.1543076855986775E-4 76-77 0.1884340334139295 2.806051832394575E-5 3.8264343169016936E-6 0.0010382391779859927 1.186194638239525E-4 78-79 0.20632771575786746 2.806051832394575E-5 3.8264343169016936E-6 0.00106183552294022 1.2180815908803723E-4 80-81 0.22935009556455932 2.86982573767627E-5 3.8264343169016936E-6 0.0010816054335775452 1.2372137624648807E-4 82-83 0.25873711111836434 3.061147453521355E-5 3.8264343169016936E-6 0.0011007376051620538 1.256345934049389E-4 84-85 0.2981009164144377 3.1249213588030494E-5 3.8264343169016936E-6 0.0011313490796972673 1.3009876677465756E-4 86-87 0.3495747485844521 3.1886952640847445E-5 3.8264343169016936E-6 0.0011677002057078334 1.3647615730282705E-4 88-89 0.41346344689565406 3.1886952640847445E-5 3.8264343169016936E-6 0.0012314741109895283 1.3775163540846096E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 58470 0.0 49.49427 1 GCGTGTT 81025 0.0 32.80165 2 GTTTTTT 1008050 0.0 31.42621 1 CGGCGAG 4295 0.0 29.959751 1 CGCGTGC 3310 0.0 26.592936 1 CGCGTAT 2190 0.0 26.05731 1 GTCGCCG 9935 0.0 25.931345 44-45 CGCCGTA 9940 0.0 25.531555 46-47 CGCGTTT 3605 0.0 25.14104 1 CCGTATC 11025 0.0 22.983397 48-49 TCGCCGT 10310 0.0 22.500761 46-47 CGTGTTT 121365 0.0 21.9364 1 GGTCGCC 11265 0.0 21.282278 44-45 CGTATCA 11400 0.0 20.678835 50-51 TGGTCGC 11900 0.0 20.611876 42-43 CGGACGT 2310 0.0 19.859823 1 CTGGAAT 144155 0.0 19.350674 1 GTGGTCG 13025 0.0 18.016546 42-43 GGAAAAC 203725 0.0 17.445576 1 GCCGTAT 13760 0.0 17.277708 48-49 >>END_MODULE