FastQCFastQC Report
Fri 27 May 2016
SRR1233067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233067_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78401973
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG996860.12714731043822072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT552850.062.457861
GCGTGTT746900.042.7676932
CGCGTAT24500.035.933771
GCGCGTT18700.033.0825421
CGCGTTT45900.033.0731161
CGGCGAG44000.030.1750951
CGCGTGC35550.029.4496521
GTTTTTT8759300.028.016121
CGTGTTT1141200.027.2675841
GCGGGTT84350.026.4596961
TATGCCG180500.023.56233448-49
TGCCGTC190550.021.90853150-51
CTCGTAT194850.021.3777544-45
CGGGTTT205100.021.2533281
CGTATGC210850.020.31866646-47
CGGACGT25250.020.1659871
CTGGAAT1797450.020.044421
CGGGGAT75200.019.6173781
CCGTCTT225400.018.90069252-53
GTGTTTT2796800.018.6230771
Produced by FastQC (version 0.11.5)