FastQCFastQC Report
Fri 27 May 2016
SRR1233066_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233066_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67311696
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTACAATTCCCCTATAGTGAGTCGTATTACAATTCCCCTATAGT1751160.2601568678346777No Hit
CGACTCACTATAGGGGAATTGTAATACGACTCACTATAGGGGAATTGTAA1623610.24120770928131122No Hit
ATACGACTCACTATAGGGGAATTGTAATACGACTCACTATAGGGGAATTG1585920.2356083852054478No Hit
CCTATAGTGAGTCGTATTACAATTCCCCTATAGTGAGTCGTATTACAATT1452600.21580202049878522No Hit
CTCACTATAGGGGAATTGTAATACGACTCACTATAGGGGAATTGTAATAC1434090.21305212692902584No Hit
GTCGTATTACAATCCCTATAGTGAGTCGTATTACAATCCCTATAGTGAGT1172920.1742520348915291No Hit
CGACTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAATACG1079320.160346576321595No Hit
ATACGACTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAAT1056430.1569459786008066No Hit
CTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAATACGACT1039080.15436841763725578No Hit
CTATAGTGAGTCGTATTACAATTCCCCTATAGTGAGTCGTATTACAATTC1035480.15383359230764293No Hit
GTCGTATTACAATTTCCCTATAGTGAGTCGTATTACAATTTCCCTATAGT1013490.15056670091925778No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT990090.14709033627677426No Hit
CCCCTATAGTGAGTCGTATTACAATTCCCCTATAGTGAGTCGTATTACAA974640.14479504423718575No Hit
GGGAATTGTAATACGACTCACTATAGGGGAATTGTAATACGACTCACTAT941680.139898421219397No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC902110.1340197994714024No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT774740.11509738218451664No Hit
GTCGTATTACAATTCCCTATAGTGAGTCGTATTACAATTCCCTATAGTGA761240.11309178719846845No Hit
CTCACTATAGGGAAATTGTAATACGACTCACTATAGGGAAATTGTAATAC732410.10880872768381886No Hit
GGGGAATTGTAATACGACTCACTATAGGGGAATTGTAATACGACTCACTA728190.10818179354743936No Hit
CTCACTATAGGGGTAATACGACTCACTATAGGGGTAATACGACTCACTAT718400.10672736577607553No Hit
GGAAATTGTAATACGACTCACTATAGGGAAATTGTAATACGACTCACTAT694480.10317374858598125No Hit
CTCACTATAGGGAATTGTAATACGACTCACTATAGGGAATTGTAATACGA683300.10151281881235023No Hit
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG682530.10139842561684971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTT2670800.031.3299981
CTCGTAT178250.019.4291481
CTTCGGG167200.018.5561811
CCCGCGT90400.018.5266341
TTCGGGC165400.017.465592
CGCGGTT71500.016.9978641
CTCGCGT534300.016.708531
CGGGTTA69900.016.59092122-23
CGCTTCG99450.016.3329644
CTGGAAT478900.015.9282371
CTTTATT833000.015.2898471
CCGAAAC102150.014.8962731
TCGGGTT83800.014.64456122-23
CCCCGCG192900.014.5290018
CGGGCCC192350.014.5047924
CTTTTAT527850.014.4417771
TCGCGTC637350.014.3336462
CGATAGG261000.014.0792782-83
CGAAACC88950.014.0455092
CCTCGGT60050.013.8217031
Produced by FastQC (version 0.11.5)