FastQCFastQC Report
Fri 27 May 2016
SRR1233066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233066_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67311696
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTACAATTCCCCTATAGTGAGTCGTATTACAATTCCCCTATAGT2164770.3216038413294474No Hit
ATACGACTCACTATAGGGGAATTGTAATACGACTCACTATAGGGGAATTG2120950.31509382856732654No Hit
CGACTCACTATAGGGGAATTGTAATACGACTCACTATAGGGGAATTGTAA2110390.3135250076004622No Hit
CCTATAGTGAGTCGTATTACAATTCCCCTATAGTGAGTCGTATTACAATT1834410.27252470358197484No Hit
CTCACTATAGGGGAATTGTAATACGACTCACTATAGGGGAATTGTAATAC1522760.22622517192257344No Hit
GTCGTATTACAATCCCTATAGTGAGTCGTATTACAATCCCTATAGTGAGT1448850.2152449107804385No Hit
ATACGACTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAAT1411650.20971838237443907No Hit
CGACTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAATACG1370670.2036302873723461No Hit
CTATAGTGAGTCGTATTACAATTCCCCTATAGTGAGTCGTATTACAATTC1274950.18940987610830665No Hit
GTCGTATTACAATTTCCCTATAGTGAGTCGTATTACAATTTCCCTATAGT1266600.18816937846878795No Hit
GGGAATTGTAATACGACTCACTATAGGGGAATTGTAATACGACTCACTAT1263900.18776825947157832No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT1234660.18342428929438948No Hit
CTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAATACGACT1112650.16529816749826062No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC1109020.16475888529090102No Hit
CCCCTATAGTGAGTCGTATTACAATTCCCCTATAGTGAGTCGTATTACAA1053910.15657160087007763No Hit
GGGGAATTGTAATACGACTCACTATAGGGGAATTGTAATACGACTCACTA1034210.15364491781636286No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT1012960.15048796274573145No Hit
GGAAATTGTAATACGACTCACTATAGGGAAATTGTAATACGACTCACTAT922110.13699105130258493No Hit
GTCGTATTACAATTCCCTATAGTGAGTCGTATTACAATTCCCTATAGTGA921830.13694945377694837No Hit
ATACGACTCACTATAGGGAATTGTAATACGACTCACTATAGGGAATTGTA823400.12232643788978367No Hit
CGACTCACTATAGGGAATTGTAATACGACTCACTATAGGGAATTGTAATA807880.12002074646878605No Hit
CTCACTATAGGGGTAATACGACTCACTATAGGGGTAATACGACTCACTAT777110.11544947552651176No Hit
CTCACTATAGGGAAATTGTAATACGACTCACTATAGGGAAATTGTAATAC773470.11490870769323655No Hit
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG754500.11209047533135995No Hit
CGACTCACTATAGGGAAATTGTAATACGACTCACTATAGGGAAATTGTAA740970.110080423467565No Hit
CTCACTATAGGGAATTGTAATACGACTCACTATAGGGAATTGTAATACGA737690.10959313816725105No Hit
GGGAAATTGTAATACGACTCACTATAGGGAAATTGTAATACGACTCACTA694340.10315294982316298No Hit
GTCGTATTACCCCTATAGTGAGTCGTATTACCCCTATAGTGAGTCGTATT686240.10194959283153407No Hit
CGACTCACTATAGGGGTAATACGACTCACTATAGGGGTAATACGACTCAC682410.1013805981058626No Hit
ATACGACTCACTATAGGGAAATTGTAATACGACTCACTATAGGGAAATTG676630.10052190632665087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCA163250.046.09081
GATTCAC229900.032.6352962
ATTCGAC180050.030.4841211
CGTCTCA167450.030.051181
CGATAGT102100.029.5336692
GTTGTAT286150.029.1705321
CCGATAG106500.028.2246441
TATGACT310200.028.0907732
ATATGAC309500.027.8610941
TGACTCA370150.027.1591434
ATGACTC339000.026.5589923
GTTTTTT2579650.025.2979871
GTCTTAT216350.024.512011
CTCTCTA325550.024.2229961
ACTAGCG20250.024.17266362-63
TTCACTA376350.022.5731094
CGATATA44800.021.48352466-67
TTCGACT272850.021.0412622
CTTACTA243400.020.537291
CGGTGAT52200.020.5009021
Produced by FastQC (version 0.11.5)