##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233066_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 67311696 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.14262426250558 31.0 31.0 34.0 28.0 34.0 2 31.301604954360382 31.0 31.0 34.0 28.0 34.0 3 30.397331468219132 31.0 31.0 34.0 25.0 34.0 4 33.13729967523029 35.0 33.0 37.0 22.0 37.0 5 34.59286604515209 35.0 35.0 37.0 30.0 37.0 6 35.026487447292965 37.0 35.0 37.0 32.0 37.0 7 35.268898543278425 37.0 35.0 37.0 33.0 37.0 8 35.359813025659015 37.0 35.0 37.0 33.0 37.0 9 36.96183884595628 39.0 37.0 39.0 33.0 39.0 10-11 36.90467078707985 39.0 37.0 39.0 33.0 39.0 12-13 36.82454780964069 39.0 37.0 39.0 32.5 39.0 14-15 38.02345494310528 40.0 37.0 41.0 33.0 41.0 16-17 37.960090576235075 40.0 37.0 41.0 33.0 41.0 18-19 37.80103250555446 40.0 37.0 41.0 32.0 41.0 20-21 37.96718354860647 40.0 37.0 41.0 33.0 41.0 22-23 37.956658780370056 40.0 37.0 41.0 33.0 41.0 24-25 37.872361097542395 40.0 37.0 41.0 33.0 41.0 26-27 37.64557582979339 40.0 37.0 41.0 32.0 41.0 28-29 37.50567631663894 40.0 37.0 41.0 32.0 41.0 30-31 37.127977506316284 39.0 36.5 40.5 31.0 41.0 32-33 37.25376736904683 39.5 37.0 41.0 31.0 41.0 34-35 37.14438105971955 39.5 36.0 41.0 31.0 41.0 36-37 37.03342053660333 39.0 36.0 41.0 31.0 41.0 38-39 36.914138985593226 39.0 36.0 41.0 30.5 41.0 40-41 36.762938784071046 39.0 36.0 41.0 30.0 41.0 42-43 36.618307344387816 39.0 36.0 40.0 30.0 41.0 44-45 36.536502125871266 39.0 35.5 40.0 30.0 41.0 46-47 36.52264633177568 39.0 35.5 40.5 30.0 41.0 48-49 36.432455505206704 39.0 35.0 40.0 30.0 41.0 50-51 36.24499950201819 39.0 35.0 40.0 30.0 41.0 52-53 36.0172780745266 39.0 35.0 40.0 29.0 41.0 54-55 35.78113661703013 38.0 35.0 40.0 28.0 41.0 56-57 35.55627444003194 38.0 34.0 40.0 28.0 41.0 58-59 35.25429353317736 38.0 34.0 40.0 28.0 41.0 60-61 34.90281114741188 38.0 34.0 40.0 26.5 41.0 62-63 34.5122698661463 37.0 33.5 40.0 26.0 41.0 64-65 33.952746585080845 36.5 33.0 39.5 25.5 41.0 66-67 31.190184615167027 35.5 28.0 39.0 15.0 40.0 68-69 29.390591755406074 35.0 25.0 38.0 9.5 40.0 70-71 31.01115829855186 34.0 27.5 37.5 16.5 40.0 72-73 31.951875102062502 35.0 31.0 37.0 22.0 39.0 74-75 31.771508060055417 35.0 31.0 37.0 21.5 39.0 76-77 30.137893814174582 33.5 28.5 35.5 20.0 37.5 78-79 31.03377862444589 34.0 30.5 36.0 20.0 39.0 80-81 31.000112692153827 34.0 31.0 36.0 20.0 37.5 82-83 30.676864069804452 34.0 30.0 35.0 19.0 37.0 84-85 30.339145696165495 34.0 30.0 35.0 18.0 37.0 86-87 30.000552407712323 34.0 30.0 35.0 15.5 36.0 88-89 29.614164943934853 34.0 29.5 35.0 9.0 36.0 90-91 29.22561804117965 34.0 29.0 35.0 4.5 36.0 92-93 28.807904973899333 34.0 29.0 35.0 2.0 35.0 94-95 28.36419837794609 34.0 29.0 35.0 2.0 35.0 96-97 27.59560738151658 33.0 27.5 35.0 2.0 35.0 98-99 26.84182913768805 33.0 26.5 35.0 2.0 35.0 100-101 23.28172202792216 29.0 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1062981330002266 1101 2 1.4147777753361162 1101 3 2.5003486193884186 1101 4 3.1761512159181677 1101 5 1.5110775003885522 1101 6 0.9815285402056659 1101 7 0.7383039755888987 1101 8 0.2629558797338447 1101 9 0.5195787430311185 1101 10-11 0.6125950008666052 1101 12-13 0.4963061098249355 1101 14-15 0.5063747596587405 1101 16-17 0.5120201894683802 1101 18-19 0.3867453419520075 1101 20-21 0.39998924208693154 1101 22-23 0.5081390061232725 1101 24-25 0.506008624565716 1101 26-27 0.5423607227570173 1101 28-29 0.5023213615408082 1101 30-31 0.5693772933853865 1101 32-33 0.5542838051475627 1101 34-35 0.47938690827310637 1101 36-37 0.2706393713734201 1101 38-39 0.4645459931647764 1101 40-41 0.3256747634896442 1101 42-43 0.020364456814654375 1101 44-45 0.08616097753279206 1101 46-47 0.4531236053257004 1101 48-49 0.21134366612108124 1101 50-51 0.39632335723573675 1101 52-53 0.3765507303965876 1101 54-55 0.4302518026703339 1101 56-57 0.48396595810811505 1101 58-59 0.3346646368709969 1101 60-61 0.343261629432277 1101 62-63 0.24615365226654262 1101 64-65 0.4553657303601639 1101 66-67 2.546077384185974 1101 68-69 3.7638454266745534 1101 70-71 2.3017712267502866 1101 72-73 0.31256093256926576 1101 74-75 0.3684622577848522 1101 76-77 -0.20144534125881108 1101 78-79 -0.2884311983195431 1101 80-81 -0.05449241257965909 1101 82-83 -0.24989585739474407 1101 84-85 0.4207113055047458 1101 86-87 0.5171462921891106 1101 88-89 0.5228366313987571 1101 90-91 0.14000299723462106 1101 92-93 0.6182649786788801 1101 94-95 0.7945565690736061 1101 96-97 0.9435062773731318 1101 98-99 0.9617921203720456 1101 100-101 0.9958048147067835 1102 1 1.014526020278037 1102 2 1.360131186421679 1102 3 2.431761343204208 1102 4 3.204857882801157 1102 5 1.4883659728322414 1102 6 0.8934595111505601 1102 7 0.6427701841941769 1102 8 0.5265343794340822 1102 9 0.32324432268080017 1102 10-11 0.46832383416595036 1102 12-13 0.4462932782731599 1102 14-15 0.48036635903388003 1102 16-17 0.5010246279279258 1102 18-19 0.4888135640495861 1102 20-21 0.499025847403189 1102 22-23 0.4892022447076556 1102 24-25 0.4550035066239886 1102 26-27 0.6076374018003818 1102 28-29 0.5632710522392799 1102 30-31 0.4109967196227515 1102 32-33 0.5422571112320753 1102 34-35 0.5165813025643757 1102 36-37 0.5134191246970445 1102 38-39 0.5392878571478192 1102 40-41 0.5379258373728106 1102 42-43 0.5216299433300762 1102 44-45 0.5627025866424162 1102 46-47 0.5568087976357674 1102 48-49 0.5051220935080352 1102 50-51 0.5653308157019552 1102 52-53 0.5185042022840349 1102 54-55 0.4538666623933878 1102 56-57 0.5170228430125619 1102 58-59 0.49865082897401436 1102 60-61 0.5438902058881254 1102 62-63 0.5937516997306318 1102 64-65 0.40032221414220714 1102 66-67 3.0366572886258147 1102 68-69 4.421602261670468 1102 70-71 2.520357738082449 1102 72-73 1.0379428384492009 1102 74-75 0.6367588969554738 1102 76-77 0.5859254422046227 1102 78-79 0.732808001042617 1102 80-81 0.6266704452783856 1102 82-83 0.6815386915511503 1102 84-85 0.621102907429389 1102 86-87 0.5800627941035614 1102 88-89 0.6227191001016337 1102 90-91 0.6906976827877251 1102 92-93 0.26455912223001476 1102 94-95 0.7077960853858123 1102 96-97 0.9461526457165981 1102 98-99 0.9842737842033529 1102 100-101 1.054364783548717 1103 1 0.8583517616421794 1103 2 1.2496506067060231 1103 3 2.0661612175278847 1103 4 1.5483512079735888 1103 5 0.9571280730894429 1103 6 0.7177713707792321 1103 7 0.5345364423455976 1103 8 0.44041699739791085 1103 9 0.45952916189887816 1103 10-11 0.47718186588232925 1103 12-13 0.4766587626169354 1103 14-15 0.4922717183097376 1103 16-17 0.4433180495264253 1103 18-19 0.44897382017748555 1103 20-21 0.4965412734143655 1103 22-23 0.5246380274722497 1103 24-25 0.4682564420385802 1103 26-27 0.5138937859246653 1103 28-29 0.5311551906793923 1103 30-31 0.3217113244769365 1103 32-33 0.4872519374477946 1103 34-35 0.5202481061737103 1103 36-37 0.4711265834854146 1103 38-39 0.47270718836664827 1103 40-41 0.50030068679105 1103 42-43 0.4574199684720597 1103 44-45 0.4583196845729347 1103 46-47 0.5554494847328968 1103 48-49 0.5692805967313745 1103 50-51 0.5228565670177119 1103 52-53 0.4729062467699592 1103 54-55 0.4489965559226121 1103 56-57 0.44338679325294805 1103 58-59 0.5509671527226132 1103 60-61 0.6076320502419819 1103 62-63 0.6266265326908069 1103 64-65 0.7002527138658721 1103 66-67 3.0680855461651753 1103 68-69 4.494578022817887 1103 70-71 2.5121082440841853 1103 72-73 1.108321691353293 1103 74-75 0.7815796547388238 1103 76-77 0.6520380793260294 1103 78-79 0.7146927054868435 1103 80-81 0.7191784146796145 1103 82-83 0.7683528403872018 1103 84-85 0.7749817278491804 1103 86-87 0.8109609274703651 1103 88-89 0.8904520367522615 1103 90-91 0.8160272275977754 1103 92-93 0.8685433512710965 1103 94-95 0.7434856963313976 1103 96-97 0.8813398108392327 1103 98-99 0.9614939065420032 1103 100-101 1.111048886366465 1104 1 0.6500175367490542 1104 2 1.0359296147746768 1104 3 2.14007445832398 1104 4 1.0225633103280742 1104 5 0.7718562755971874 1104 6 0.6301540215893553 1104 7 0.42807420795128337 1104 8 0.35430337498419107 1104 9 0.3824589959146465 1104 10-11 0.3830287613715697 1104 12-13 0.3593866831736392 1104 14-15 0.37651762473576866 1104 16-17 0.41365773015865415 1104 18-19 0.40007783553629395 1104 20-21 0.48761215076933695 1104 22-23 0.4591578683759252 1104 24-25 0.404822319900525 1104 26-27 0.5043749444627039 1104 28-29 0.46652529749582783 1104 30-31 0.19272323641690292 1104 32-33 0.3774697686384698 1104 34-35 0.4265309604549188 1104 36-37 0.37553591716636703 1104 38-39 0.38657247261007655 1104 40-41 0.4409977994947667 1104 42-43 0.48998352817113044 1104 44-45 0.5016265443594037 1104 46-47 0.478094264586602 1104 48-49 0.45976891054088753 1104 50-51 0.4752013131870285 1104 52-53 0.4173020637274192 1104 54-55 0.37359060836174507 1104 56-57 0.47487029299801264 1104 58-59 0.5434733982240729 1104 60-61 0.4940187432551042 1104 62-63 0.542484661384421 1104 64-65 0.5604313617748744 1104 66-67 2.8982080189518733 1104 68-69 4.237996757858792 1104 70-71 2.1576758795201663 1104 72-73 0.7281502126560007 1104 74-75 0.39731921700419903 1104 76-77 0.25161125095636905 1104 78-79 0.42110221636886536 1104 80-81 0.3954324524512707 1104 82-83 0.33447427294827037 1104 84-85 0.4484958288900458 1104 86-87 0.46966940150183945 1104 88-89 0.4157584555687883 1104 90-91 0.5598440951482395 1104 92-93 0.605595633438309 1104 94-95 0.6041910740980896 1104 96-97 0.534925269995302 1104 98-99 0.587088163951087 1104 100-101 0.7557721141095506 1105 1 0.902712771822479 1105 2 1.2248663158773034 1105 3 2.257358482551993 1105 4 3.1189777134123204 1105 5 1.303111110483826 1105 6 0.8020966686408215 1105 7 0.48853535333511644 1105 8 0.2679816303775695 1105 9 0.3216982101626513 1105 10-11 0.2924948870982007 1105 12-13 0.31290908705569365 1105 14-15 0.3365894743623059 1105 16-17 0.3161755618877109 1105 18-19 0.2551790148606088 1105 20-21 0.31327447952169507 1105 22-23 0.2811216258709024 1105 24-25 0.2658754072165337 1105 26-27 0.3064942924251852 1105 28-29 0.22937528567852894 1105 30-31 0.11343835499869215 1105 32-33 0.19144131331423608 1105 34-35 0.1436922315634419 1105 36-37 0.21283967677935323 1105 38-39 0.18315418550387363 1105 40-41 0.23556560562739293 1105 42-43 0.15881845598531896 1105 44-45 0.11768965670201936 1105 46-47 0.104586898039841 1105 48-49 0.1824414651202062 1105 50-51 0.2203920658776184 1105 52-53 0.24319147190821866 1105 54-55 0.24472623917854008 1105 56-57 0.27514590064683375 1105 58-59 0.299588881761359 1105 60-61 0.1773170122376868 1105 62-63 0.07099953870842768 1105 64-65 0.1687241969529154 1105 66-67 2.426126152459627 1105 68-69 3.8479316864972724 1105 70-71 1.8520216757594419 1105 72-73 0.4940886076668356 1105 74-75 0.09260565500140316 1105 76-77 -0.06047376924320247 1105 78-79 0.10004438073593391 1105 80-81 0.047984009161197605 1105 82-83 0.06462460430220673 1105 84-85 0.08572822842696581 1105 86-87 0.2066404516733975 1105 88-89 0.19361276656591997 1105 90-91 0.19285696627639126 1105 92-93 0.29202620255771805 1105 94-95 0.3000401326228612 1105 96-97 0.3669521162202649 1105 98-99 0.38234509489151236 1105 100-101 0.35346274725836935 1106 1 0.1956681778479954 1106 2 0.2891948367156658 1106 3 -0.6457923311641487 1106 4 -2.5457021494669583 1106 5 -0.7213645990856108 1106 6 -0.07939034638638276 1106 7 0.06746201956388376 1106 8 0.15172234869082502 1106 9 0.21415020210070423 1106 10-11 0.26363301129692474 1106 12-13 0.27315028957326604 1106 14-15 0.33752756969280284 1106 16-17 0.2706662518434584 1106 18-19 0.294439801679772 1106 20-21 0.3411891879470801 1106 22-23 0.31814987970482633 1106 24-25 0.3391169311281814 1106 26-27 0.361638389263355 1106 28-29 0.3708441384687049 1106 30-31 0.15618709280528265 1106 32-33 0.2871745534953334 1106 34-35 0.2198715221771792 1106 36-37 0.2255872166049997 1106 38-39 0.3150009371697209 1106 40-41 0.3369370409860508 1106 42-43 0.4073213419138284 1106 44-45 0.3825296575704371 1106 46-47 0.41996393878663696 1106 48-49 0.34797106098481123 1106 50-51 0.37218984101126296 1106 52-53 0.35634685796708254 1106 54-55 0.2840794341002777 1106 56-57 0.36642873289029154 1106 58-59 0.45180217080460494 1106 60-61 0.4829722708093911 1106 62-63 0.48036211612154034 1106 64-65 0.4830466086150409 1106 66-67 2.8672898255308716 1106 68-69 4.251856400259403 1106 70-71 2.2121568666173737 1106 72-73 0.8101155556166297 1106 74-75 0.5593966804827666 1106 76-77 0.4302769158190749 1106 78-79 0.501724074170447 1106 80-81 0.5213486796738245 1106 82-83 0.5906577252664178 1106 84-85 0.5907266248000198 1106 86-87 0.5138862247923761 1106 88-89 0.5637109171968646 1106 90-91 0.6499391667974059 1106 92-93 0.6796755172145978 1106 94-95 0.7287249723266456 1106 96-97 0.8317990445072176 1106 98-99 0.836883962735989 1106 100-101 0.859577551744227 1107 1 -0.005721739147219296 1107 2 -0.06587189077038857 1107 3 -2.8242689882084804 1107 4 -1.8987496094803795 1107 5 -0.6246569525129217 1107 6 -0.21522036633342623 1107 7 -0.0699538653254379 1107 8 0.05787243140183307 1107 9 0.18953762797867313 1107 10-11 0.03871990855376595 1107 12-13 0.0711630617280008 1107 14-15 0.12442468729635436 1107 16-17 0.09385552673733599 1107 18-19 0.15578651352615225 1107 20-21 0.22744402830258537 1107 22-23 0.1697158126287519 1107 24-25 0.23635394404981724 1107 26-27 0.23740265569730212 1107 28-29 0.23886142252324305 1107 30-31 0.06553449401869926 1107 32-33 0.21393519653670978 1107 34-35 0.1452285018843682 1107 36-37 0.22749237608446293 1107 38-39 0.2288741223158297 1107 40-41 0.2286669292879111 1107 42-43 0.2557324656320077 1107 44-45 0.2705965207535712 1107 46-47 0.31452028906433327 1107 48-49 0.3308666885079177 1107 50-51 0.20222388891338028 1107 52-53 0.15377656991577737 1107 54-55 0.045066536475410146 1107 56-57 0.16957932249516006 1107 58-59 0.2635998744204926 1107 60-61 0.2327833921388205 1107 62-63 0.2206231343353977 1107 64-65 0.22716910768451015 1107 66-67 2.7754433721112513 1107 68-69 4.144168056106512 1107 70-71 2.12325883244387 1107 72-73 0.7241867105316793 1107 74-75 0.4794699710124313 1107 76-77 0.2787874670453263 1107 78-79 0.3329201008338565 1107 80-81 0.3683558856857907 1107 82-83 0.44304573560718197 1107 84-85 0.34720022259036654 1107 86-87 0.3069733147319056 1107 88-89 0.3601025657033148 1107 90-91 0.2959992444284545 1107 92-93 0.27932240263174535 1107 94-95 0.24514376348859201 1107 96-97 0.4764465259183801 1107 98-99 0.6476214771910733 1107 100-101 0.8522088740299658 1108 1 -0.5190950765250193 1108 2 0.299707865968859 1108 3 -4.135929832384534 1108 4 -5.475674123313489 1108 5 -2.419199016200942 1108 6 -0.9838955081333012 1108 7 -0.47537128277211593 1108 8 -0.21834852504540692 1108 9 -0.09941258113569518 1108 10-11 -0.04194630099038221 1108 12-13 -0.04659624518208716 1108 14-15 -0.0800188726733353 1108 16-17 -0.2068766767114809 1108 18-19 -0.1686827767003507 1108 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-0.8812162423008729 2316 54-55 -0.9676382092533302 2316 56-57 -0.8867251515744314 2316 58-59 -0.9084983161581093 2316 60-61 -0.990944494722676 2316 62-63 -1.0897460135780506 2316 64-65 -0.9740832392597198 2316 66-67 1.278910422268698 2316 68-69 2.775096723682477 2316 70-71 0.7466708399478996 2316 72-73 -0.6833664928844847 2316 74-75 -1.1245595350169388 2316 76-77 -1.1227064138955782 2316 78-79 -1.2605475914557118 2316 80-81 -1.322566245504973 2316 82-83 -1.484668429012988 2316 84-85 -1.5483156814355397 2316 86-87 -1.601296403743703 2316 88-89 -1.7273913168260542 2316 90-91 -1.7808542254479143 2316 92-93 -1.9522744090110429 2316 94-95 -2.099860232109293 2316 96-97 -2.613899085804583 2316 98-99 -2.9241400305662353 2316 100-101 -3.8007095929796115 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7218.0 3 1087.0 4 2277.0 5 4613.0 6 11254.0 7 30528.0 8 71826.0 9 136132.0 10 208814.0 11 229991.0 12 205315.0 13 197050.0 14 201434.0 15 222264.0 16 256619.0 17 295567.0 18 335772.0 19 372038.0 20 394185.0 21 411074.0 22 436305.0 23 487180.0 24 573524.0 25 699698.0 26 875722.0 27 1095808.0 28 1367279.0 29 1691713.0 30 2076924.0 31 2544879.0 32 3151062.0 33 3978055.0 34 5220299.0 35 7241991.0 36 1.0358913E7 37 1.2293922E7 38 8678964.0 39 944009.0 40 391.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.38913250580484 19.644825242030308 13.06413426437345 41.901907987791404 2 20.526421284699182 17.106914198833064 46.26622911158763 16.100435404880127 3 18.969309583285497 27.757225133652852 27.950193083829 25.323272199232655 4 23.140595060923737 30.303149693331154 23.03786105760877 23.518394188136337 5 20.3203675592187 30.910874201832893 30.774715901827264 17.99404233712114 6 14.948394703945656 39.300138864425584 27.0785466466333 18.672919784995464 7 12.268236414664102 21.79218898302607 46.55742443334068 19.382150168969147 8 14.938231537057096 20.376148299695195 41.48272240830182 23.202897754945887 9 17.12915198247277 25.169504888633572 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22.21213599423682 34-35 20.84971110159791 28.570471591923308 28.542154846393913 22.037662460084864 36-37 20.988106914322966 29.552718667735988 28.54601289923359 20.913161518707447 38-39 21.02219563810116 28.616239401135857 29.76745541421079 20.594109546552193 40-41 21.585874287425046 28.38978298732682 29.42731226288759 20.597030462360543 42-43 21.197670461311805 29.312445938072933 29.355291389478584 20.134592211136678 44-45 21.182230351171068 28.934361570684537 30.03935229919032 19.844055778954075 46-47 21.39881455371441 29.776549531599976 28.51523975862976 20.30939615605585 48-49 20.85218889745402 29.66725054736401 29.169517582798687 20.311042972383284 50-51 20.487093357445634 28.85870978499784 29.58240422288572 21.0717926346708 52-53 20.73671235841454 29.207448862785167 28.512188767815566 21.543650010984727 54-55 20.384938925644047 29.28004397575284 29.29684196220508 21.03817513639803 56-57 20.675930318014462 29.448595605693257 29.35384757181914 20.52162650447314 58-59 21.39276211373429 29.212706213790838 29.268626658879608 20.12590501359526 60-61 22.024414598021718 29.357484173329993 28.31049005213002 20.307611176518268 62-63 21.47437051652955 29.746860040489842 28.247165990290902 20.53160345268971 64-65 21.50231662562774 28.483933163710507 29.324132614337927 20.689617596323824 66-67 21.832675659451322 29.458823679373648 27.67619821734631 21.03230244382872 68-69 22.23295000578483 29.045512018803887 28.3607657476962 20.36077222771509 70-71 21.006969276676553 28.5541661119228 29.503425534994832 20.93543907640581 72-73 19.808155860476333 31.11483294958498 27.924112787880627 21.152898402058057 74-75 20.031856618680393 29.03412529624702 30.271225424563315 20.662792660509265 76-77 21.120646372840376 28.25034759884919 29.39466864714356 21.234337381166874 78-79 20.5534546976049 30.522944478470315 27.117678854485494 21.805921969439297 80-81 20.63967658885904 28.799576681387556 29.271965010990247 21.288781718763154 82-83 21.145454249648594 28.809304391165742 28.754852661182788 21.290388698002875 84-85 20.723133904156185 30.254000456958412 28.20988157154915 20.812984067336252 86-87 21.19681674426059 28.97436394838407 29.306974720465394 20.521844586889944 88-89 21.796091432153972 29.084235922504774 28.944119919879157 20.1755527254621 90-91 21.32143901793023 30.328799718799758 28.159968191470586 20.189793071799425 92-93 21.37635119603548 29.400690696308274 29.230044512137642 19.99291359551861 94-95 21.706732987326287 28.88991870007639 28.609053784325233 20.794294528272093 96-97 20.36811148956191 30.210338553731347 27.90272599739249 21.51882395931425 98-99 20.352115329258677 29.615812978475535 29.21639873700404 20.815672955261743 100-101 20.276327719931345 29.803443077607568 28.032533751331044 21.887695451130046 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7338.0 1 9647.0 2 9834.5 3 7731.5 4 8129.0 5 10379.5 6 11968.5 7 12433.0 8 13992.5 9 15319.5 10 16697.5 11 18454.0 12 20856.5 13 23422.5 14 26981.5 15 31741.0 16 35242.5 17 39561.0 18 48197.0 19 57804.5 20 67207.5 21 83795.0 22 106993.0 23 131201.0 24 165757.0 25 208797.0 26 257648.5 27 323090.0 28 418044.0 29 522268.5 30 652805.0 31 824299.5 32 1328612.0 33 1758343.5 34 2010324.0 35 2636226.5 36 3386939.0 37 3560315.0 38 3574451.5 39 3736677.0 40 3756481.0 41 3314718.5 42 2995958.0 43 3339416.5 44 3346058.5 45 3225363.0 46 3154623.5 47 2817487.0 48 2460247.0 49 2154430.0 50 1774738.0 51 1448327.5 52 1222947.5 53 1035751.5 54 889474.5 55 772082.0 56 652026.0 57 536345.0 58 447652.5 59 381326.0 60 303009.5 61 230673.5 62 179675.0 63 133192.0 64 87121.0 65 58389.0 66 42556.0 67 30716.5 68 23139.0 69 20017.0 70 23308.5 71 30993.0 72 31479.5 73 36643.0 74 38347.0 75 28267.0 76 22905.0 77 23648.0 78 22054.5 79 15172.5 80 10492.5 81 7164.0 82 5008.0 83 3119.5 84 1535.0 85 694.5 86 396.5 87 209.5 88 120.5 89 93.0 90 75.0 91 63.0 92 42.0 93 23.0 94 14.5 95 8.5 96 6.5 97 3.0 98 1.5 99 2.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16662334581496804 2 1.3964883606557767E-4 3 0.0 4 0.0 5 0.0015940766074294132 6 0.0 7 0.0 8 0.0 9 9.775418524590436E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.005532470909661822 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.004815656405389042 28-29 0.013240640972707031 30-31 0.09440632724511948 32-33 0.03747788497262051 34-35 0.0025270496824207193 36-37 3.3426583100803165E-5 38-39 2.9712518311825035E-6 40-41 7.428129577956259E-6 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 7.725254761074509E-5 54-55 8.468067718870135E-5 56-57 1.3370633240321266E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 7.027010580746621E-4 68-69 0.04151061652049296 70-71 0.008704282239449143 72-73 0.008397500487879551 74-75 0.008535663698029538 76-77 0.008945696450732723 78-79 0.007527666514300872 80-81 0.008049864023631198 82-83 0.010593998404081217 84-85 0.011853066367544803 86-87 0.019854647548919285 88-89 0.025401974717737016 90-91 0.03204272256042991 92-93 0.03729663861091838 94-95 0.04889492013393928 96-97 0.023765557771713255 98-99 0.0 100-101 0.08767421340861772 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6.7311696E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.39429147537425 #Duplication Level Percentage of deduplicated Percentage of total 1 69.95235461421603 12.167716455484143 2 10.58968163445541 3.6840001796227 3 4.603972826578666 2.4024853587063593 4 2.727211874232482 1.89751673022006 5 1.841217370560682 1.6013335806527331 6 1.3373505016776457 1.3957358658551369 7 1.064029468666261 1.2955627101457974 8 0.8105797661422064 1.1279568573054586 9 0.6437833809496725 1.007834019771645 >10 5.247002947533491 18.346939245344924 >50 0.6507333477463664 7.902465393094676 >100 0.45704027132457864 15.400533622846503 >500 0.04261325628891067 5.107036954097481 >1k 0.026410043472010956 8.764644376830226 >5k 0.003327988208392842 4.01019206919376 >10k+ 0.002690707947010447 13.888046580828622 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCGTATTACAATTCCCCTATAGTGAGTCGTATTACAATTCCCCTATAGT 216477 0.3216038413294474 No Hit ATACGACTCACTATAGGGGAATTGTAATACGACTCACTATAGGGGAATTG 212095 0.31509382856732654 No Hit CGACTCACTATAGGGGAATTGTAATACGACTCACTATAGGGGAATTGTAA 211039 0.3135250076004622 No Hit CCTATAGTGAGTCGTATTACAATTCCCCTATAGTGAGTCGTATTACAATT 183441 0.27252470358197484 No Hit CTCACTATAGGGGAATTGTAATACGACTCACTATAGGGGAATTGTAATAC 152276 0.22622517192257344 No Hit GTCGTATTACAATCCCTATAGTGAGTCGTATTACAATCCCTATAGTGAGT 144885 0.2152449107804385 No Hit ATACGACTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAAT 141165 0.20971838237443907 No Hit CGACTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAATACG 137067 0.2036302873723461 No Hit CTATAGTGAGTCGTATTACAATTCCCCTATAGTGAGTCGTATTACAATTC 127495 0.18940987610830665 No Hit GTCGTATTACAATTTCCCTATAGTGAGTCGTATTACAATTTCCCTATAGT 126660 0.18816937846878795 No Hit GGGAATTGTAATACGACTCACTATAGGGGAATTGTAATACGACTCACTAT 126390 0.18776825947157832 No Hit ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 123466 0.18342428929438948 No Hit CTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAATACGACT 111265 0.16529816749826062 No Hit GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC 110902 0.16475888529090102 No Hit CCCCTATAGTGAGTCGTATTACAATTCCCCTATAGTGAGTCGTATTACAA 105391 0.15657160087007763 No Hit GGGGAATTGTAATACGACTCACTATAGGGGAATTGTAATACGACTCACTA 103421 0.15364491781636286 No Hit ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 101296 0.15048796274573145 No Hit GGAAATTGTAATACGACTCACTATAGGGAAATTGTAATACGACTCACTAT 92211 0.13699105130258493 No Hit GTCGTATTACAATTCCCTATAGTGAGTCGTATTACAATTCCCTATAGTGA 92183 0.13694945377694837 No Hit ATACGACTCACTATAGGGAATTGTAATACGACTCACTATAGGGAATTGTA 82340 0.12232643788978367 No Hit CGACTCACTATAGGGAATTGTAATACGACTCACTATAGGGAATTGTAATA 80788 0.12002074646878605 No Hit CTCACTATAGGGGTAATACGACTCACTATAGGGGTAATACGACTCACTAT 77711 0.11544947552651176 No Hit CTCACTATAGGGAAATTGTAATACGACTCACTATAGGGAAATTGTAATAC 77347 0.11490870769323655 No Hit CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG 75450 0.11209047533135995 No Hit CGACTCACTATAGGGAAATTGTAATACGACTCACTATAGGGAAATTGTAA 74097 0.110080423467565 No Hit CTCACTATAGGGAATTGTAATACGACTCACTATAGGGAATTGTAATACGA 73769 0.10959313816725105 No Hit GGGAAATTGTAATACGACTCACTATAGGGAAATTGTAATACGACTCACTA 69434 0.10315294982316298 No Hit GTCGTATTACCCCTATAGTGAGTCGTATTACCCCTATAGTGAGTCGTATT 68624 0.10194959283153407 No Hit CGACTCACTATAGGGGTAATACGACTCACTATAGGGGTAATACGACTCAC 68241 0.1013805981058626 No Hit ATACGACTCACTATAGGGAAATTGTAATACGACTCACTATAGGGAAATTG 67663 0.10052190632665087 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.01087181045029678 2.9712518311825035E-6 0.0 0.0 0.0 2 0.010940149242413977 1.782751098709502E-5 0.0 1.4856259155912518E-6 0.0 3 0.011088711833973103 1.782751098709502E-5 0.0 1.4856259155912518E-6 0.0 4 0.011770614129229489 1.9313136902686273E-5 0.0 2.9712518311825035E-6 0.0 5 0.011937004231775707 1.9313136902686273E-5 0.0 2.9712518311825035E-6 0.0 6 0.012170247500523534 2.525564056505128E-5 0.0 2.9712518311825035E-6 0.0 7 0.012296525703348792 2.9712518311825035E-5 0.0 4.456877746773755E-6 0.0 8 0.01260553589379177 3.565502197419004E-5 0.0 4.456877746773755E-6 0.0 9 0.012905632328741205 3.565502197419004E-5 0.0 4.456877746773755E-6 0.0 10-11 0.01402208020430803 3.788346084757692E-5 0.0 4.456877746773755E-6 0.0 12-13 0.015899168548657576 4.0854712678759423E-5 0.0 5.942503662365007E-6 0.0 14-15 0.018172919012469986 4.30831515521463E-5 0.0 7.428129577956259E-6 0.0 16-17 0.02157945923692073 4.30831515521463E-5 0.0 7.428129577956259E-6 0.0 18-19 0.02456259607542796 4.30831515521463E-5 0.0 7.428129577956259E-6 0.0 20-21 0.027654183605773357 4.30831515521463E-5 0.0 7.428129577956259E-6 0.0 22-23 0.031329622120946116 4.30831515521463E-5 0.0 7.428129577956259E-6 0.0 24-25 0.03474061922314363 4.30831515521463E-5 0.0 7.428129577956259E-6 0.0 26-27 0.03872135386397038 4.531159042553318E-5 0.0 7.428129577956259E-6 0.0 28-29 0.04365734596852232 5.051128113010256E-5 0.0 7.428129577956259E-6 0.0 30-31 0.0485851671305385 5.199690704569381E-5 0.0 7.428129577956259E-6 0.0 32-33 0.05460640896642985 5.199690704569381E-5 0.0 7.428129577956259E-6 0.0 34-35 0.06126646994602543 5.199690704569381E-5 0.0 7.428129577956259E-6 0.0 36-37 0.0690006384625935 5.199690704569381E-5 0.0 7.428129577956259E-6 0.0 38-39 0.07863937940294952 5.199690704569381E-5 0.0 7.428129577956259E-6 0.0 40-41 0.0887460925067168 5.199690704569381E-5 0.0 8.170942535751885E-6 0.0 42-43 0.09977909336885524 5.199690704569381E-5 0.0 1.1142194366934388E-5 0.0 44-45 0.1121981832102403 5.273972000348944E-5 0.0 1.4113446198116892E-5 0.0 46-47 0.12565498275366588 5.3482532961285063E-5 0.0 1.8570323944890647E-5 0.0 48-49 0.14027428457604157 5.4968158876876315E-5 0.0 1.9313136902686273E-5 0.0 50-51 0.155519777721839 5.645378479246757E-5 0.0 1.9313136902686273E-5 0.0 52-53 0.17163139077642614 5.8682223665854445E-5 0.0 1.9313136902686273E-5 0.0 54-55 0.18910829404744162 6.462472732821946E-5 0.0 2.0798762818277525E-5 0.0 56-57 0.20611351109025688 6.536754028601508E-5 0.0 2.6741266480642532E-5 0.0 58-59 0.22268121130093052 6.61103532438107E-5 0.0 3.3426583100803165E-5 0.0 60-61 0.2402791930840667 6.833879211719758E-5 0.0 3.565502197419004E-5 0.0 62-63 0.2594519383377296 6.833879211719758E-5 0.0 3.565502197419004E-5 0.0 64-65 0.28033463902023803 7.20528569061757E-5 0.0 3.565502197419004E-5 0.0 66-67 0.3025870867969216 4.055758749564117E-4 0.0 3.565502197419004E-5 0.0 68-69 0.32887820862513995 4.0706150087200296E-4 0.0 3.8626273805372546E-5 0.0 70-71 0.3595600978468883 4.0854712678759425E-4 0.0 3.936908676316817E-5 0.0 72-73 0.3916028204073182 4.0854712678759425E-4 0.0 4.159752563655505E-5 0.0 74-75 0.42665244982090483 4.0854712678759425E-4 0.0 4.159752563655505E-5 0.0 76-77 0.4631305085523324 4.115183786187767E-4 0.0 4.159752563655505E-5 0.0 78-79 0.5042986882992816 4.115183786187767E-4 0.0 4.159752563655505E-5 0.0 80-81 0.5523052041356973 4.13004004534368E-4 0.0 4.382596450994193E-5 0.0 82-83 0.6044283893842164 4.13004004534368E-4 0.0 4.456877746773755E-5 0.0 84-85 0.6650746105104826 4.13004004534368E-4 0.0 4.679721634112443E-5 0.0 86-87 0.7370502148690474 4.13004004534368E-4 0.0 5.051128113010256E-5 0.0 88-89 0.8183288087110447 4.13004004534368E-4 0.0 5.051128113010256E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATTCA 16325 0.0 46.0908 1 GATTCAC 22990 0.0 32.635296 2 ATTCGAC 18005 0.0 30.484121 1 CGTCTCA 16745 0.0 30.05118 1 CGATAGT 10210 0.0 29.533669 2 GTTGTAT 28615 0.0 29.170532 1 CCGATAG 10650 0.0 28.224644 1 TATGACT 31020 0.0 28.090773 2 ATATGAC 30950 0.0 27.861094 1 TGACTCA 37015 0.0 27.159143 4 ATGACTC 33900 0.0 26.558992 3 GTTTTTT 257965 0.0 25.297987 1 GTCTTAT 21635 0.0 24.51201 1 CTCTCTA 32555 0.0 24.222996 1 ACTAGCG 2025 0.0 24.172663 62-63 TTCACTA 37635 0.0 22.573109 4 CGATATA 4480 0.0 21.483524 66-67 TTCGACT 27285 0.0 21.041262 2 CTTACTA 24340 0.0 20.53729 1 CGGTGAT 5220 0.0 20.500902 1 >>END_MODULE