FastQCFastQC Report
Fri 27 May 2016
SRR1233065_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233065_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66320657
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG1030660.15540557748093478No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC921740.13898233848919803No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA841530.12688806746893355No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN697960.10524021195990263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT525500.060.633261
GCGTGTT684050.043.359852
CGCGTGC33900.037.730851
GTTTTTT8716000.036.8234181
CGCGTTT36250.030.8250521
CGGCGAG48500.030.6864411
CGTGTTT1013000.028.2982543
CTGGAAT1688050.024.700671
CGGACGT23350.024.0291881
CGCGGGT13800.018.606241
CTGGCGA60500.016.740491
GGAAAAC2636100.016.7119641
CAAAACG211500.016.4359269
CGCCGAT5250.016.302611
TATGGCG2315000.016.272447
CGGCAAG84750.015.9339181
TGGCGAG2316200.015.9328819
ATGGCGA2371700.015.8901448
CGCGTGG20000.015.6912641
CTCGCGT30400.015.1719751
Produced by FastQC (version 0.11.5)