##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233065_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 66320657 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.487437375658086 33.0 31.0 34.0 30.0 34.0 2 31.755345005101503 33.0 31.0 34.0 30.0 34.0 3 31.742069925513555 34.0 31.0 34.0 30.0 34.0 4 35.171438877633555 37.0 35.0 37.0 32.0 37.0 5 35.15446683527276 37.0 35.0 37.0 32.0 37.0 6 35.18090375672846 37.0 35.0 37.0 32.0 37.0 7 35.27003592560912 37.0 35.0 37.0 33.0 37.0 8 35.27395860990943 37.0 35.0 37.0 32.0 37.0 9 36.84854257399772 39.0 37.0 39.0 33.0 39.0 10-11 36.738011212705565 39.0 37.0 39.0 32.0 39.0 12-13 36.66457767449439 39.0 37.0 39.0 32.0 39.0 14-15 37.87565432290576 40.0 38.0 41.0 32.0 41.0 16-17 37.66675586461696 40.0 37.5 41.0 32.0 41.0 18-19 37.436863570274944 40.0 37.0 41.0 32.0 41.0 20-21 37.28627182478002 40.0 37.0 41.0 31.0 41.0 22-23 37.066713045378904 40.0 37.0 41.0 31.0 41.0 24-25 36.787275117012186 40.0 36.0 41.0 30.0 41.0 26-27 36.582280201174726 40.0 36.0 41.0 30.0 41.0 28-29 36.44663720686603 40.0 36.0 41.0 29.5 41.0 30-31 36.2756630336156 40.0 36.0 41.0 28.5 41.0 32-33 36.19124869345006 40.0 36.0 41.0 28.0 41.0 34-35 36.06641986372361 40.0 36.0 41.0 27.0 41.0 36-37 35.93868297324015 40.0 36.0 41.0 27.0 41.0 38-39 35.80193289550796 40.0 36.0 41.0 26.5 41.0 40-41 35.65538361599765 39.0 35.0 41.0 26.0 41.0 42-43 35.52781552661639 39.0 35.0 41.0 25.5 41.0 44-45 35.3889859459625 39.0 35.0 41.0 25.0 41.0 46-47 35.33466581912782 39.0 35.0 41.0 25.0 41.0 48-49 35.3033237668318 39.0 35.0 41.0 24.0 41.0 50-51 34.958314910842944 38.5 34.5 40.5 24.0 41.0 52-53 35.0590369453065 39.0 35.0 40.5 24.0 41.0 54-55 35.247413238080554 39.0 35.0 41.0 25.0 41.0 56-57 35.15608421973263 39.0 35.0 41.0 24.5 41.0 58-59 34.94496436457196 39.0 35.0 41.0 23.5 41.0 60-61 34.73007518607664 39.0 34.0 41.0 23.0 41.0 62-63 34.48440092353126 38.5 34.0 40.5 23.0 41.0 64-65 34.198661851314284 38.0 34.0 40.0 22.0 41.0 66-67 33.92253672939338 38.0 34.0 40.0 22.0 41.0 68-69 33.504814993011905 37.0 33.0 40.0 20.0 41.0 70-71 33.211240157044884 37.0 33.0 39.5 20.0 41.0 72-73 32.85496878446183 36.0 32.5 39.0 19.0 41.0 74-75 32.46503725830098 36.0 32.0 39.0 18.0 40.5 76-77 32.04373003271063 35.0 32.0 38.0 17.5 40.0 78-79 31.51495173668138 35.0 31.0 37.0 10.5 39.0 80-81 31.176291008395772 35.0 31.0 37.0 10.5 39.0 82-83 30.826807716938028 35.0 31.0 36.0 7.5 38.5 84-85 30.454391909899204 35.0 31.0 36.0 7.0 37.0 86-87 30.105230803126695 35.0 30.5 35.5 2.0 37.0 88-89 29.72173969868845 34.0 30.0 35.0 2.0 36.5 90-91 29.41381953589513 34.0 30.0 35.0 2.0 36.0 92-93 29.128566639501173 34.0 30.0 35.0 2.0 36.0 94-95 28.845082407733084 34.0 29.0 35.0 2.0 36.0 96-97 28.468277372463305 34.0 29.0 35.0 2.0 35.0 98-99 27.966670949897257 34.0 29.0 35.0 2.0 35.0 100-101 26.115089918364347 32.5 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.44093740540999704 1101 2 0.4608532448850369 1101 3 0.4421748345840264 1101 4 0.4020940172031331 1101 5 0.41434158098714136 1101 6 0.4614576747114967 1101 7 0.4993813586618856 1101 8 0.4683919989914358 1101 9 0.5622711489097441 1101 10-11 0.5877372643711425 1101 12-13 0.576864260679379 1101 14-15 0.7053806971329237 1101 16-17 0.731736147060218 1101 18-19 0.6819012416644483 1101 20-21 0.7095544793207083 1101 22-23 0.7437357375654443 1101 24-25 0.7531730045579863 1101 26-27 0.6723153492646077 1101 28-29 0.6847731755713724 1101 30-31 0.6731011905243136 1101 32-33 0.5684964201454292 1101 34-35 0.6066501722204336 1101 36-37 0.6610199578720852 1101 38-39 0.7020807157340556 1101 40-41 0.650792863528757 1101 42-43 0.6086374431106023 1101 44-45 0.5473535975675432 1101 46-47 0.6175929076881772 1101 48-49 0.7727120892530266 1101 50-51 0.6756786933450059 1101 52-53 0.6668160870996402 1101 54-55 0.6655223381947337 1101 56-57 0.6034886248844131 1101 58-59 0.6211802195047369 1101 60-61 0.6427640920466899 1101 62-63 0.6485291351322644 1101 64-65 0.624610043388337 1101 66-67 0.560587492182151 1101 68-69 0.67946907864971 1101 70-71 0.6230366084302759 1101 72-73 0.5826673159247449 1101 74-75 0.4877006869391707 1101 76-77 0.48523753206276155 1101 78-79 0.6443811090767646 1101 80-81 0.5914967472827932 1101 82-83 0.48898705331318837 1101 84-85 0.48296719995203574 1101 86-87 0.4748810417368041 1101 88-89 0.5359246916928697 1101 90-91 0.5014783582344613 1101 92-93 0.47192139159172797 1101 94-95 0.48147567967647475 1101 96-97 0.5380762308702352 1101 98-99 0.5620026599027419 1101 100-101 0.9705312257756908 1102 1 0.3870603998168214 1102 2 0.347777913534145 1102 3 0.3418393160946529 1102 4 0.36267284842249126 1102 5 0.3809613156161973 1102 6 0.42839359611972583 1102 7 0.4059548335807648 1102 8 0.42195317830887547 1102 9 0.49144071108496945 1102 10-11 0.4712771615858671 1102 12-13 0.4931487087352835 1102 14-15 0.6024863894869057 1102 16-17 0.6124580095137802 1102 18-19 0.6482415849757857 1102 20-21 0.6883512143443369 1102 22-23 0.6444552702942019 1102 24-25 0.6145086392810128 1102 26-27 0.5293302101350719 1102 28-29 0.5481307202701302 1102 30-31 0.5878973288114153 1102 32-33 0.5701860446817832 1102 34-35 0.5330905878437093 1102 36-37 0.5380170332936274 1102 38-39 0.5959383283084918 1102 40-41 0.5527469081569691 1102 42-43 0.5708408808071539 1102 44-45 0.5435839129313678 1102 46-47 0.5403415854901255 1102 48-49 0.6750205604318538 1102 50-51 0.4562535266500447 1102 52-53 0.4926519372981133 1102 54-55 0.5686974412849395 1102 56-57 0.5845020244071009 1102 58-59 0.5863618519323026 1102 60-61 0.5316712322835357 1102 62-63 0.5454515975921481 1102 64-65 0.5570311250511324 1102 66-67 0.5737357106705758 1102 68-69 0.5768846604535867 1102 70-71 0.5289684182163583 1102 72-73 0.4462726791873024 1102 74-75 0.4611877171274017 1102 76-77 0.41230248086852583 1102 78-79 0.5532418486167394 1102 80-81 0.5047045797229934 1102 82-83 0.4263790848277118 1102 84-85 0.41586821617680414 1102 86-87 0.34886501214631593 1102 88-89 0.44425034997675183 1102 90-91 0.42037859861907734 1102 92-93 0.3957991822557858 1102 94-95 0.38405148023293023 1102 96-97 0.38253589453016446 1102 98-99 0.4920453453475524 1102 100-101 0.8168859894544305 1103 1 0.30738783434054184 1103 2 0.25446601022207815 1103 3 0.3394580478682947 1103 4 0.3019205370941549 1103 5 0.337347040175068 1103 6 0.35903357626709465 1103 7 0.30271557234649293 1103 8 0.3119253992862383 1103 9 0.37795073292425485 1103 10-11 0.3606761821444593 1103 12-13 0.374292968726742 1103 14-15 0.5276684480302407 1103 16-17 0.5609235796037098 1103 18-19 0.5293427955962287 1103 20-21 0.5506475885238089 1103 22-23 0.5153508808609573 1103 24-25 0.4814096617934496 1103 26-27 0.3448494542448941 1103 28-29 0.4186057891445003 1103 30-31 0.5304709058851032 1103 32-33 0.5013101494188774 1103 34-35 0.46792900854034514 1103 36-37 0.4862792446634572 1103 38-39 0.456585721142595 1103 40-41 0.42745236279251486 1103 42-43 0.2843199627139583 1103 44-45 0.31610255134403076 1103 46-47 0.17862148843767045 1103 48-49 0.38692447311537137 1103 50-51 0.399161748804147 1103 52-53 0.4233737064859113 1103 54-55 0.3747784490060795 1103 56-57 0.4048823708048843 1103 58-59 0.5068430597852966 1103 60-61 0.49677616175053885 1103 62-63 0.4605454517757579 1103 64-65 0.3991545945800894 1103 66-67 0.39149762242590214 1103 68-69 0.49510921903618765 1103 70-71 0.46058311426126153 1103 72-73 0.4033377715341757 1103 74-75 0.4054510105283029 1103 76-77 0.33065546422732695 1103 78-79 0.4133585258827246 1103 80-81 0.3838380686656713 1103 82-83 0.4080288937512826 1103 84-85 0.2860907596382276 1103 86-87 0.2963325621867874 1103 88-89 0.37659472975625974 1103 90-91 0.2249130630645162 1103 92-93 0.2745560826872122 1103 94-95 0.3865117481049225 1103 96-97 0.41579383142677884 1103 98-99 0.34804561654833677 1103 100-101 0.6295912265402883 1104 1 0.29769064667265965 1104 2 0.31242384305232207 1104 3 0.31664981926753555 1104 4 0.30643998622602453 1104 5 0.2985186954474841 1104 6 0.35177873468071397 1104 7 0.3574257703431556 1104 8 0.38853224313202617 1104 9 0.4451563054600953 1104 10-11 0.400163473065291 1104 12-13 0.4338226302102939 1104 14-15 0.5414098688840383 1104 16-17 0.5025562704463127 1104 18-19 0.5561454567428754 1104 20-21 0.5628854513710806 1104 22-23 0.5702844603473025 1104 24-25 0.5664124388314136 1104 26-27 0.5362369915080336 1104 28-29 0.519293033410527 1104 30-31 0.4987417533253833 1104 32-33 0.46783725920244734 1104 34-35 0.42796231448290456 1104 36-37 0.3977125933131731 1104 38-39 0.5088082920572035 1104 40-41 0.5263170538542781 1104 42-43 0.5014837382188944 1104 44-45 0.5087206442581333 1104 46-47 0.4682474102998242 1104 48-49 0.5183887816731314 1104 50-51 0.4664587057144871 1104 52-53 0.4974480327377293 1104 54-55 0.5276285679666515 1104 56-57 0.43713227327522475 1104 58-59 0.5368531342603262 1104 60-61 0.5053285974615846 1104 62-63 0.4913148604627082 1104 64-65 0.4071768184989608 1104 66-67 0.41353567680695846 1104 68-69 0.5237546373341857 1104 70-71 0.41785453224046165 1104 72-73 0.4377332157398115 1104 74-75 0.4333852304497654 1104 76-77 0.3880920230813061 1104 78-79 0.5216638340407975 1104 80-81 0.49660789476409306 1104 82-83 0.3596943653611966 1104 84-85 0.32531597367068343 1104 86-87 0.33462805366916015 1104 88-89 0.4278994310518627 1104 90-91 0.3258166083860097 1104 92-93 0.3770416199294644 1104 94-95 0.44111492308317324 1104 96-97 0.42667631049553734 1104 98-99 0.5010953528832083 1104 100-101 0.7880114016950017 1105 1 0.23822768804938832 1105 2 0.22262855130280457 1105 3 0.21084264662360042 1105 4 0.2035436889716351 1105 5 0.21162140674077534 1105 6 0.2777379866580745 1105 7 0.29217433803938775 1105 8 0.2661986480479541 1105 9 0.3357939728543471 1105 10-11 0.2691897951096678 1105 12-13 0.35144138097589206 1105 14-15 0.4448332173884495 1105 16-17 0.47111880600256484 1105 18-19 0.48470150250846444 1105 20-21 0.4550577953310935 1105 22-23 0.41187241251024886 1105 24-25 0.47302770752341416 1105 26-27 0.43148002841554955 1105 28-29 0.3968547359630179 1105 30-31 0.4253613395055069 1105 32-33 0.378760381588755 1105 34-35 0.39242887658625847 1105 36-37 0.4268459853924753 1105 38-39 0.48167164676001306 1105 40-41 0.4371099133621428 1105 42-43 0.4008604817274062 1105 44-45 0.3188800455152574 1105 46-47 0.3694538700618466 1105 48-49 0.49085439315424395 1105 50-51 0.49478202836411356 1105 52-53 0.4757005815715374 1105 54-55 0.45905001349002106 1105 56-57 0.443092807858136 1105 58-59 0.4304303671538321 1105 60-61 0.4295573981735714 1105 62-63 0.3550162076592329 1105 64-65 0.4135600874276193 1105 66-67 0.3878601801752666 1105 68-69 0.4656341893235023 1105 70-71 0.4597729336719212 1105 72-73 0.3942796803798174 1105 74-75 0.37918374786605824 1105 76-77 0.3557681460085078 1105 78-79 0.5761921719055358 1105 80-81 0.4266924127895848 1105 82-83 0.3801670180106207 1105 84-85 0.29082293864544795 1105 86-87 0.3401760497044357 1105 88-89 0.34845656228463184 1105 90-91 0.292116977313281 1105 92-93 0.3610315820528456 1105 94-95 0.3607627850594781 1105 96-97 0.38474758602659875 1105 98-99 0.3565893763292074 1105 100-101 0.6267083939962639 1106 1 0.47656779419948947 1106 2 0.44595845172238313 1106 3 0.43715458142824914 1106 4 0.4114215828555672 1106 5 0.4189966202605575 1106 6 0.4368548067386371 1106 7 0.4557207606068303 1106 8 0.42982471635365016 1106 9 0.5184339595625502 1106 10-11 0.530496625103936 1106 12-13 0.5801953582706076 1106 14-15 0.7437253809885718 1106 16-17 0.7489828368518516 1106 18-19 0.7364465315242015 1106 20-21 0.7691368039332644 1106 22-23 0.7205785990463127 1106 24-25 0.7545917063027403 1106 26-27 0.7344621726223295 1106 28-29 0.735309770619132 1106 30-31 0.8108423583840576 1106 32-33 0.7338714761548815 1106 34-35 0.7661201764782888 1106 36-37 0.7726535183250576 1106 38-39 0.795767482556677 1106 40-41 0.7773238137359968 1106 42-43 0.7336357571145271 1106 44-45 0.6576296027844322 1106 46-47 0.7444338371392121 1106 48-49 0.9095234403391927 1106 50-51 0.8825144815258028 1106 52-53 0.7784773890065821 1106 54-55 0.8064431539213572 1106 56-57 0.8291425867440978 1106 58-59 0.8403929692129424 1106 60-61 0.8224250155393449 1106 62-63 0.8181068972834282 1106 64-65 0.8576432665969023 1106 66-67 0.8324608662972679 1106 68-69 0.95664268340024 1106 70-71 0.8470829640339304 1106 72-73 0.8583775339749522 1106 74-75 0.8161531655402356 1106 76-77 0.8252961701801595 1106 78-79 1.000060993458451 1106 80-81 0.8964020361916347 1106 82-83 0.8561238251974466 1106 84-85 0.8971551944748981 1106 86-87 0.848897800573063 1106 88-89 0.8830120552910365 1106 90-91 0.8585688641932627 1106 92-93 0.9456653245179787 1106 94-95 0.9952850483588307 1106 96-97 0.9825681717148811 1106 98-99 1.0020771872594132 1106 100-101 1.2167879993934605 1107 1 0.15497593816615307 1107 2 0.1403120803399318 1107 3 0.07996412751912985 1107 4 0.19022720649260094 1107 5 0.208730910622684 1107 6 0.24300853046295146 1107 7 0.29459712659753734 1107 8 0.2817676554868811 1107 9 0.2889183809209612 1107 10-11 0.27562691256355265 1107 12-13 0.2752645077036888 1107 14-15 0.36122475040044577 1107 16-17 0.3386874043240482 1107 18-19 0.35113442862452615 1107 20-21 0.3361542154403807 1107 22-23 0.3165425567292104 1107 24-25 0.3394774013823465 1107 26-27 0.3226159626429137 1107 28-29 0.29311959623857575 1107 30-31 0.3397814398740948 1107 32-33 0.23807114796814943 1107 34-35 0.28771052563859456 1107 36-37 0.30354831745957256 1107 38-39 0.31247195551461004 1107 40-41 0.2949185085892978 1107 42-43 0.274392316982933 1107 44-45 0.2868817838752449 1107 46-47 0.25686130044091016 1107 48-49 0.3690049585227442 1107 50-51 0.32615441567459413 1107 52-53 0.29616646227422905 1107 54-55 0.30884192006733 1107 56-57 0.30697201652540684 1107 58-59 0.3128422029188158 1107 60-61 0.28672903623991175 1107 62-63 0.3128812995453245 1107 64-65 0.2906160609628259 1107 66-67 0.28895120994500445 1107 68-69 0.4262789642156548 1107 70-71 0.3389517057641811 1107 72-73 0.3121712548049729 1107 74-75 0.2622382313784115 1107 76-77 0.23682450926893495 1107 78-79 0.4429529361647049 1107 80-81 0.3762941155090722 1107 82-83 0.3422865001902302 1107 84-85 0.3245989784803065 1107 86-87 0.3050158902668336 1107 88-89 0.32615011986330344 1107 90-91 0.2687972709995101 1107 92-93 0.33207174482522817 1107 94-95 0.24074517470363688 1107 96-97 0.3401861511639197 1107 98-99 0.3694695371325345 1107 100-101 0.6200911490175471 1108 1 -0.03165401907950027 1108 2 -1.365534390949108E-4 1108 3 -0.04727797592039806 1108 4 0.0013902825780078842 1108 5 0.07544096340047446 1108 6 0.10434146714815284 1108 7 0.1086832010556833 1108 8 0.11493398411991507 1108 9 0.09973971923714231 1108 10-11 0.07659575794874485 1108 12-13 0.09575924489748644 1108 14-15 0.08529201344175164 1108 16-17 0.09044238764923307 1108 18-19 0.1680146686923436 1108 20-21 0.15961272145136007 1108 22-23 0.10300597472564021 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-1.3705063293863589 2316 62-63 -1.555644011930724 2316 64-65 -1.6013046202062569 2316 66-67 -1.5964955380997452 2316 68-69 -1.3404285789710926 2316 70-71 -1.3221302595496525 2316 72-73 -1.6409492175758942 2316 74-75 -1.5263789647309949 2316 76-77 -1.3351774460824295 2316 78-79 -1.1570260005066224 2316 80-81 -1.018553344862351 2316 82-83 -1.1811932912175465 2316 84-85 -1.4660674814860073 2316 86-87 -1.288145375422328 2316 88-89 -1.50354384982138 2316 90-91 -1.2134539134883475 2316 92-93 -1.4082171287543694 2316 94-95 -1.5847125281540748 2316 96-97 -1.5501283920796958 2316 98-99 -1.7203393131417428 2316 100-101 -1.8274979747372981 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 245620.0 3 37076.0 4 44003.0 5 66584.0 6 126903.0 7 227327.0 8 354660.0 9 522583.0 10 594792.0 11 498459.0 12 367978.0 13 288672.0 14 253011.0 15 236269.0 16 237499.0 17 242720.0 18 255310.0 19 273674.0 20 303166.0 21 340901.0 22 387645.0 23 442974.0 24 510240.0 25 589883.0 26 690210.0 27 814818.0 28 967654.0 29 1159000.0 30 1399112.0 31 1709307.0 32 2113094.0 33 2667086.0 34 3469353.0 35 4709393.0 36 6956278.0 37 1.1679485E7 38 1.5666315E7 39 4862312.0 40 9291.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.83134827402633 14.179956224248178 12.930960689844634 41.05773481188086 2 17.28296212245763 18.861082023255662 50.87018440430825 12.985771449978456 3 20.879665663149076 22.31623028986087 36.41597476219158 20.388129284798474 4 21.064960312217433 30.40473551704616 32.24396420045012 16.28633997028629 5 20.109688688703248 31.345610905679518 33.90106632339106 14.643634082226175 6 15.849909125211001 33.1442402006871 35.28757707011751 15.718273603984395 7 13.444525983666422 16.190238908980227 52.12180339992365 18.243431707429696 8 15.239012777364255 21.132167362115556 43.00896099032317 20.61985887019702 9 18.393667501482796 20.16282171226374 40.86671405222064 20.57679673403283 10-11 20.436130218950403 31.6067280573742 30.69687056195061 17.26027116172478 12-13 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31.30899266535185 20.498430599177723 38-39 20.278915793579333 27.808419909284588 31.847961894720957 20.06470240241513 40-41 20.918939928879713 28.29980890866835 30.98305821917314 19.798192943278796 42-43 20.7828495509227 29.261590423867222 30.676775822865316 19.278784202344763 44-45 20.471859558645164 28.985750415293808 30.68349627391055 19.85889375215048 46-47 20.286420994365976 28.8101593065582 30.62740818314 20.276011515935828 48-49 21.044056628395147 28.783259249080967 29.795229380798066 20.377454741725824 50-51 20.45417236852277 28.98070320242591 30.50344782859841 20.06167660045291 52-53 20.235223762023587 29.28594240041948 30.966535931133937 19.512297906422997 54-55 20.220542552780984 29.2794443202619 30.755868025669226 19.744145101287888 56-57 21.172523516796687 28.32913860952718 30.120324909563134 20.378012964112997 58-59 20.692730790763903 28.820759284143165 29.542608403894814 20.94390152119811 60-61 21.346917086475116 28.796284066975765 29.810296348435745 20.046502498113377 62-63 19.578272112937018 30.737335589269232 29.866818737045392 19.817573560748357 64-65 19.29588398333564 30.051877074852417 30.365897665952744 20.286341275859197 66-67 19.93500431926485 28.619357251422155 30.84508865470406 20.600549774608936 68-69 21.058892477893846 29.65283777649907 29.03333875048509 20.254930995122 70-71 20.04605835993939 30.498303974689662 29.244253496913803 20.211384168457148 72-73 19.620924911401072 30.215180881785397 30.013004871601655 20.150889335211875 74-75 20.065718884444465 29.237229672401917 30.24368775190297 20.453363691250647 76-77 20.68918697980242 29.315276050895278 29.39180597838506 20.603730990917242 78-79 20.48971186224651 30.274110578041576 28.830857356384126 20.40532020332779 80-81 20.1333570000709 30.006311549676635 30.39088671871949 19.469444731532974 82-83 19.92021246265836 30.0648739154356 30.00693607654758 20.00797754535846 84-85 20.128017592491958 30.12156990600748 29.394004921803997 20.35640757969657 86-87 20.61208070976579 30.378929123288557 28.685663315922476 20.323326851023175 88-89 20.170506369561608 30.183923648258244 29.456511135824755 20.189058846355397 90-91 20.10419150482491 30.485560111793035 29.072270150384583 20.337978232997475 92-93 19.780927358506254 30.77893004271169 28.996639420968418 20.44350317781364 94-95 19.553255333361243 30.736663588334036 28.745912123789193 20.96416895451553 96-97 19.932346716847647 29.95614754136132 29.322038804747528 20.789466937043503 98-99 20.56599549664071 30.236473466150283 28.7849718275064 20.412559209702607 100-101 20.8158295600221 30.968120034367054 27.744769659035732 20.47128074657512 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 73465.0 1 45973.5 2 15813.0 3 13438.0 4 14646.0 5 17376.0 6 20561.0 7 24082.0 8 28423.0 9 33158.0 10 38278.0 11 43844.5 12 50359.0 13 57589.5 14 65604.0 15 74727.0 16 85319.5 17 98282.0 18 115308.5 19 135975.5 20 161328.5 21 194928.0 22 237546.5 23 293197.0 24 365064.0 25 453044.0 26 556115.5 27 686028.0 28 850953.0 29 1048076.0 30 1294657.0 31 1589821.5 32 2007682.5 33 2494520.5 34 2967647.5 35 3428483.5 36 3736552.5 37 3722404.5 38 3397923.5 39 2987789.5 40 2720977.0 41 2536337.0 42 2354487.0 43 2272394.0 44 2208782.0 45 2123248.0 46 2045881.5 47 1958687.5 48 1867526.0 49 1765019.5 50 1625996.0 51 1463821.0 52 1317067.0 53 1189979.0 54 1060936.0 55 926149.5 56 793938.5 57 655821.0 58 524299.0 59 406431.0 60 297656.5 61 212681.5 62 150648.5 63 102557.0 64 68973.0 65 46407.0 66 30936.0 67 21418.5 68 15330.0 69 10526.0 70 7826.5 71 7036.5 72 6150.5 73 6987.5 74 6904.5 75 4652.0 76 3746.5 77 3327.0 78 2903.0 79 2175.0 80 1439.5 81 863.0 82 603.0 83 472.0 84 324.5 85 220.5 86 195.5 87 151.0 88 105.0 89 80.5 90 75.5 91 70.0 92 57.0 93 45.0 94 25.5 95 18.5 96 15.5 97 11.0 98 5.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.370504471932478 2 0.29881790827253113 3 0.2788904820409122 4 0.27771588571566774 5 0.275834119073941 6 0.20451244926599563 7 0.2087811042040793 8 0.20877808855240984 9 0.2261708595558696 10-11 0.21021203092122565 12-13 0.217012325435799 14-15 0.2135450799288674 16-17 0.22761007931510693 18-19 0.23346270529256066 20-21 0.2212214212534113 22-23 0.19756137216795064 24-25 0.18118186012542065 26-27 0.16925495777280974 28-29 0.16608098439073063 30-31 0.1863235462218054 32-33 0.20403522238930777 34-35 0.21185631499398444 36-37 0.20242712613658215 38-39 0.20288098171283192 40-41 0.18347752495877717 42-43 0.1751890063453382 44-45 0.1726716911142783 46-47 0.17576951929170426 48-49 0.2075914296204876 50-51 0.21177866196349654 52-53 0.22758972366633823 54-55 0.23988604334845479 56-57 0.23999988419897592 58-59 0.2245665328677308 60-61 0.2044770153588798 62-63 0.19771893996767853 64-65 0.1923164000018878 66-67 0.19339147982204097 68-69 0.3949862860978594 70-71 0.20626755853760617 72-73 0.2274374332570318 74-75 0.22369048605776026 76-77 0.20815761822142384 78-79 0.20654726022994616 80-81 0.19497092738390695 82-83 0.1750314385456103 84-85 0.1694992255580339 86-87 0.19292405381327873 88-89 0.1716863269312908 90-91 0.15439910373626123 92-93 0.20518569350119675 94-95 0.20264274523094666 96-97 0.20059210209573164 98-99 0.2058310429584556 100-101 0.17222085118969796 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6.6320657E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.93464370412101 #Duplication Level Percentage of deduplicated Percentage of total 1 75.62853134483257 33.227125785011665 2 10.790528081532235 9.481560132828623 3 4.526882856177384 5.966609561293409 4 2.4213423636342166 4.25523256127854 5 1.492999660456679 3.279720406626892 6 1.0386518673338028 2.7379679834358353 7 0.725054574019209 2.2298510062904024 8 0.5058260854149063 1.7778631071163349 9 0.40343038977682466 1.5952113390833509 >10 2.2472199569398974 17.734689573607362 >50 0.13789742165005503 4.144163185774732 >100 0.07154612102917532 5.88089074979565 >500 0.006000693709627479 1.8138980401734797 >1k 0.0035892208690844027 2.9984721037753492 >5k 3.2717469341706244E-4 0.9905576737472017 >10k+ 1.7218793053250814E-4 1.8861867901612608 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG 103066 0.15540557748093478 No Hit CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 92174 0.13898233848919803 No Hit AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA 84153 0.12688806746893355 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 69796 0.10524021195990263 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.010321067838637364 1.5078258347169269E-6 0.0 1.3570432512452342E-5 6.0313033388677075E-6 2 0.01035725565867057 9.046955008301562E-6 0.0 4.523477504150781E-5 6.0313033388677075E-6 3 0.010416060866224531 9.046955008301562E-6 0.0 5.1266078380375514E-5 6.0313033388677075E-6 4 0.010719133859002633 9.046955008301562E-6 0.0 9.650085342188332E-5 6.0313033388677075E-6 5 0.010797540802407914 9.046955008301562E-6 0.0 1.0705563426490181E-4 1.5078258347169268E-5 6 0.010963401644226775 9.046955008301562E-6 0.0 1.2062606677735415E-4 1.6586084181886197E-5 7 0.01102522250345017 9.046955008301562E-6 0.0 1.23641718446788E-4 1.6586084181886197E-5 8 0.011171481609417711 9.046955008301562E-6 0.0 1.296730217856557E-4 1.6586084181886197E-5 9 0.011337342451236573 9.046955008301562E-6 0.0 1.341964992898065E-4 1.6586084181886197E-5 10-11 0.012041497116049378 1.2062606677735415E-5 0.0 1.4173562846339112E-4 1.733999709924466E-5 12-13 0.013288469081360277 1.2062606677735415E-5 0.0 1.5681388681056037E-4 1.8847822933961587E-5 14-15 0.014806849696920223 1.2816519595093878E-5 0.0 1.6661475473622042E-4 2.0355648768678513E-5 16-17 0.016454149421348464 1.3570432512452342E-5 1.5078258347169269E-6 1.85462577670182E-4 2.186347460339544E-5 18-19 0.018187395218355572 1.3570432512452342E-5 1.5078258347169269E-6 2.0732605227357745E-4 2.412521335547083E-5 20-21 0.01986786711114759 1.4324345429810805E-5 1.5078258347169269E-6 2.2391213645546365E-4 2.5633039190187757E-5 22-23 0.02131387208664112 1.5832171264527733E-5 1.5078258347169269E-6 2.495451756456514E-4 2.638695210754622E-5 24-25 0.02277646314631654 1.733999709924466E-5 1.5078258347169269E-6 3.0835038319961157E-4 3.015651669433854E-5 26-27 0.024128229007140265 1.8847822933961587E-5 1.5078258347169269E-6 3.294599448856485E-4 4.297303628943242E-5 28-29 0.02547396356462512 1.960173585132005E-5 1.5078258347169269E-6 3.505695065716855E-4 4.900433962830013E-5 30-31 0.026797080734589224 2.1109561686036977E-5 1.5078258347169269E-6 3.701712424230056E-4 5.1266078380375514E-5 32-33 0.0282513787521737 2.1109561686036977E-5 1.5078258347169269E-6 3.7695645867923176E-4 5.277390421509244E-5 34-35 0.029793884581089114 2.5633039190187757E-5 2.2617387520753904E-6 3.942964557784764E-4 5.277390421509244E-5 36-37 0.03145852430261661 2.864869085962161E-5 3.0156516694338538E-6 4.1314427871243795E-4 5.428173004980937E-5 38-39 0.03328299356262408 2.864869085962161E-5 3.0156516694338538E-6 4.22945146638098E-4 5.5789555884526294E-5 40-41 0.03534042191409534 2.9402603776980074E-5 3.0156516694338538E-6 4.500860116630027E-4 5.6543468801884754E-5 42-43 0.03772504847170015 3.166434252905547E-5 3.0156516694338538E-6 4.666720958448889E-4 5.805129463660169E-5 44-45 0.040490401052571 3.241825544641393E-5 3.0156516694338538E-6 4.779807896052658E-4 5.880520755396015E-5 46-47 0.04365457356672446 3.3172168363772394E-5 3.0156516694338538E-6 5.066294804648875E-4 5.880520755396015E-5 48-49 0.04721907383999528 3.392608128113086E-5 3.0156516694338538E-6 5.179381742252643E-4 6.634433672754477E-5 50-51 0.051134897532755144 3.467999419848932E-5 3.0156516694338538E-6 5.360320842418675E-4 6.785216256226171E-5 52-53 0.05517360903104443 3.618782003320625E-5 3.0156516694338538E-6 5.488486038369613E-4 6.935998839697864E-5 54-55 0.05932766920568956 3.7695645867923174E-5 3.0156516694338538E-6 5.66188600936206E-4 7.463737881848787E-5 56-57 0.06371167282012903 3.8449558785281634E-5 3.0156516694338538E-6 5.918216401263938E-4 7.689911757056327E-5 58-59 0.06827661553473452 3.92034717026401E-5 3.0156516694338538E-6 6.091616372256385E-4 7.765303048792174E-5 60-61 0.07325168687638303 3.92034717026401E-5 3.0156516694338538E-6 6.29517285994317E-4 8.142259507471405E-5 62-63 0.07915331719346508 3.995738461999857E-5 3.0156516694338538E-6 6.498729347629954E-4 8.745389841358176E-5 64-65 0.08578548912746747 4.071129753735703E-5 3.0156516694338538E-6 6.626894543580894E-4 8.745389841358176E-5 66-67 0.09335552873066381 4.2219123372073954E-5 3.0156516694338538E-6 6.807833643746924E-4 8.745389841358176E-5 68-69 0.10230145940803934 4.2219123372073954E-5 3.0156516694338538E-6 7.034007518954464E-4 9.197737591773253E-5 70-71 0.11300626892161217 4.372694920679088E-5 3.0156516694338538E-6 7.199868360773326E-4 9.197737591773253E-5 72-73 0.1253463155529355 4.448086212414934E-5 3.0156516694338538E-6 7.282798781682757E-4 9.499302758716639E-5 74-75 0.1403657686925508 4.523477504150781E-5 3.0156516694338538E-6 7.43358136515445E-4 9.650085342188332E-5 76-77 0.15866172737100598 4.523477504150781E-5 3.0156516694338538E-6 7.82561608218085E-4 9.650085342188332E-5 78-79 0.18043397851140106 4.523477504150781E-5 3.0156516694338538E-6 8.436285545241205E-4 9.650085342188332E-5 80-81 0.20761857048551252 4.523477504150781E-5 3.0156516694338538E-6 8.798163745573268E-4 9.650085342188332E-5 82-83 0.24172785260556148 4.523477504150781E-5 3.0156516694338538E-6 9.167581075078915E-4 9.725476633924178E-5 84-85 0.2875175075542451 4.6742600876224734E-5 3.0156516694338538E-6 9.318363658550608E-4 9.800867925660025E-5 86-87 0.34695826369753846 4.975825254565859E-5 3.0156516694338538E-6 9.514381017063809E-4 9.800867925660025E-5 88-89 0.41921026204550416 5.1266078380375514E-5 3.0156516694338538E-6 9.650085342188333E-4 9.800867925660025E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 52550 0.0 60.63326 1 GCGTGTT 68405 0.0 43.35985 2 CGCGTGC 3390 0.0 37.73085 1 GTTTTTT 871600 0.0 36.823418 1 CGCGTTT 3625 0.0 30.825052 1 CGGCGAG 4850 0.0 30.686441 1 CGTGTTT 101300 0.0 28.298254 3 CTGGAAT 168805 0.0 24.70067 1 CGGACGT 2335 0.0 24.029188 1 CGCGGGT 1380 0.0 18.60624 1 CTGGCGA 6050 0.0 16.74049 1 GGAAAAC 263610 0.0 16.711964 1 CAAAACG 21150 0.0 16.435926 9 CGCCGAT 525 0.0 16.30261 1 TATGGCG 231500 0.0 16.27244 7 CGGCAAG 8475 0.0 15.933918 1 TGGCGAG 231620 0.0 15.932881 9 ATGGCGA 237170 0.0 15.890144 8 CGCGTGG 2000 0.0 15.691264 1 CTCGCGT 3040 0.0 15.171975 1 >>END_MODULE