FastQCFastQC Report
Fri 27 May 2016
SRR1233065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233065_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66320657
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG1025100.15456722631683217No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC964470.14542527828094345No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA907710.13686685884309016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT502600.060.639241
GCGTGTT642100.044.4796942
CGCGTTT38450.036.3894841
CGCGTGC31850.034.217711
CGGCGAG53750.031.1665781
GTTTTTT7247300.029.6008151
CGTGTTT948350.029.1013453
CGGACGT25550.024.5871351
CTGGAAT1711700.024.494761
GCGCGTT14000.022.0958251
CGGGGAT59850.017.811841
CGGCAAG85750.017.1494121
CGGGTTT154700.017.104461
GTGTTTT2307800.017.1037271
CCGATAG31000.016.4265731
CCCGTAT37350.016.3096371
GCGGGTT62500.016.2951621
CTGGCGA58350.016.1491341
CGATAGT28650.015.77856352
TATGGCG2454400.015.7335287
Produced by FastQC (version 0.11.5)