FastQCFastQC Report
Fri 27 May 2016
SRR1233064_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233064_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61874740
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3930790.6352818613864075Illumina Single End PCR Primer 1 (100% over 50bp)
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC3538320.5718520999037733No Hit
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA863510.13955775814169077No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA752830.12167000620931902No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN659560.1065960034741156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT414900.064.487351
GCGTGTT509200.052.5485462
CTGGAAT3007250.046.8560521
CGTGTTT1215350.046.1663251
AGAGCGT1004250.044.882089
AAGAGCG1075000.042.206758
GTCGCCG587650.041.75536344-45
CCGTATC605900.041.18691648-49
CGCCGTA609000.040.85613346-47
TGGTCGC595100.040.00793542-43
TCGGTGG657000.038.07567638-39
CGTATCA600100.037.75768750-51
GGTCGCC602600.037.4801844-45
CGGAAGA1212650.037.4773455
TCGCCGT595650.037.41718346-47
TCTCGGT657800.037.3684236-37
GTGGTCG599850.037.10257342-43
GATCTCG697900.036.95658534-35
TCGGAAG1254650.036.320534
CGGCGAG54350.036.1383861
Produced by FastQC (version 0.11.5)