##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233064_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 61874740 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02343924192651 34.0 31.0 34.0 30.0 34.0 2 32.265965303450166 34.0 31.0 34.0 30.0 34.0 3 32.23007716880911 34.0 31.0 34.0 30.0 34.0 4 35.59882431829209 37.0 35.0 37.0 33.0 37.0 5 35.573494078520575 37.0 35.0 37.0 33.0 37.0 6 35.62073102852634 37.0 35.0 37.0 33.0 37.0 7 35.70134095108925 37.0 35.0 37.0 35.0 37.0 8 35.6735060220051 37.0 35.0 37.0 35.0 37.0 9 37.364685492011766 39.0 38.0 39.0 35.0 39.0 10-11 37.250073664956005 39.0 37.0 39.0 34.0 39.0 12-13 37.196675444616005 39.0 37.0 39.0 34.0 39.0 14-15 38.518101110404665 40.0 38.0 41.0 34.0 41.0 16-17 38.35952909862732 40.0 38.0 41.0 33.5 41.0 18-19 38.12577340931048 40.0 38.0 41.0 33.0 41.0 20-21 37.832569817667114 40.0 38.0 41.0 33.0 41.0 22-23 37.53353711870143 40.0 38.0 41.0 32.0 41.0 24-25 37.22957940510133 40.0 37.5 41.0 31.5 41.0 26-27 37.04673748932117 40.0 38.0 41.0 31.0 41.0 28-29 36.76442920002573 40.0 38.0 41.0 30.0 41.0 30-31 36.54995056948926 40.0 37.5 41.0 29.5 41.0 32-33 36.41685489102662 40.0 37.0 41.0 29.0 41.0 34-35 36.330139948870894 40.0 37.0 41.0 28.5 41.0 36-37 36.220773218602616 40.0 37.0 41.0 27.5 41.0 38-39 36.057475069794236 40.0 37.0 41.0 27.0 41.0 40-41 35.96861013880624 40.0 37.0 41.0 26.5 41.0 42-43 35.80837338468008 40.0 37.0 41.0 25.0 41.0 44-45 35.70963903040239 40.0 36.5 41.0 25.0 41.0 46-47 35.757841915780176 40.0 37.0 41.0 25.0 41.0 48-49 35.7694525342652 40.0 37.0 41.0 25.0 41.0 50-51 35.38206538726466 39.5 36.0 40.5 24.5 41.0 52-53 35.4359847411076 39.5 36.0 40.5 24.5 41.0 54-55 35.647654099233385 40.0 36.5 41.0 25.5 41.0 56-57 35.569681165528934 40.0 36.0 41.0 25.0 41.0 58-59 35.43038021654717 40.0 36.0 41.0 24.5 41.0 60-61 35.202568164326834 39.5 36.0 41.0 23.0 41.0 62-63 34.97743418396587 39.0 35.0 41.0 23.0 41.0 64-65 34.716559107965544 39.0 35.0 41.0 22.0 41.0 66-67 34.329690072233035 39.0 35.0 41.0 19.5 41.0 68-69 34.00196480825616 38.0 34.0 40.0 18.0 41.0 70-71 33.65656250838387 37.5 34.0 40.0 15.5 41.0 72-73 33.24339198839462 37.0 34.0 40.0 13.0 41.0 74-75 32.877918226403864 36.5 33.5 39.0 10.0 41.0 76-77 32.45782497025442 36.0 33.0 39.0 8.0 40.5 78-79 32.086196588462435 35.5 33.0 38.5 7.0 40.0 80-81 31.68707156587648 35.0 32.0 37.0 6.5 39.0 82-83 31.273526628152297 35.0 32.0 37.0 2.0 39.0 84-85 30.926152045244958 35.0 32.0 36.5 2.0 38.5 86-87 30.53033853556395 35.0 31.5 36.0 2.0 37.0 88-89 30.06666220334825 35.0 31.0 36.0 2.0 37.0 90-91 29.789435002070313 35.0 31.0 35.0 2.0 36.5 92-93 29.55013473996012 35.0 31.0 35.0 2.0 36.0 94-95 29.300613546012478 34.0 31.0 35.0 2.0 36.0 96-97 28.97850563089235 34.0 31.0 35.0 2.0 36.0 98-99 28.59317083190976 34.0 30.0 35.0 2.0 35.5 100-101 26.881238862579465 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.292298658059849 1101 2 0.24926121289217207 1101 3 0.2825395228587695 1101 4 0.23062943846181838 1101 5 0.23893238622578394 1101 6 0.25449212620527817 1101 7 0.22716707367884226 1101 8 0.26487250516436234 1101 9 0.3165175996731264 1101 10-11 0.35747412336546347 1101 12-13 0.37452193292151037 1101 14-15 0.5250249453416416 1101 16-17 0.5184376531485313 1101 18-19 0.4839391260986403 1101 20-21 0.5511757149271759 1101 22-23 0.5341224368238073 1101 24-25 0.5445099121595689 1101 26-27 0.43058382447026844 1101 28-29 0.48396993892562534 1101 30-31 0.44104696467975657 1101 32-33 0.42604129299970595 1101 34-35 0.46044976322101405 1101 36-37 0.4775265818362726 1101 38-39 0.5647301461412724 1101 40-41 0.4845333276179531 1101 42-43 0.5067165250834123 1101 44-45 0.4502055400963769 1101 46-47 0.47086763676859533 1101 48-49 0.5067485536435612 1101 50-51 0.4533009814436113 1101 52-53 0.4555695265162072 1101 54-55 0.5177627433738081 1101 56-57 0.5120415813552057 1101 58-59 0.5303003776664212 1101 60-61 0.4954431464073821 1101 62-63 0.5939309983662966 1101 64-65 0.507636281564686 1101 66-67 0.5154256060909859 1101 68-69 0.5559047067347365 1101 70-71 0.5623117565472882 1101 72-73 0.6231427183631197 1101 74-75 0.5439657914318161 1101 76-77 0.5038634755960771 1101 78-79 0.5576225125257182 1101 80-81 0.5671940472976367 1101 82-83 0.5417495241471428 1101 84-85 0.6022471887214813 1101 86-87 0.611310973749962 1101 88-89 0.5620539587655102 1101 90-91 0.5650055379526222 1101 92-93 0.5413443678829175 1101 94-95 0.516542844157609 1101 96-97 0.5751266168147815 1101 98-99 0.6637278397526778 1101 100-101 0.9293786606511638 1102 1 0.2002685293906552 1102 2 0.20316679864124154 1102 3 0.237866541548442 1102 4 0.21157513706762643 1102 5 0.19416751107040398 1102 6 0.19728525470826952 1102 7 0.179236703786529 1102 8 0.21685946047465876 1102 9 0.29992504869075987 1102 10-11 0.3025124182241754 1102 12-13 0.30607951610369355 1102 14-15 0.3955016888104339 1102 16-17 0.4458828754462232 1102 18-19 0.46230439201125506 1102 20-21 0.4876605316009588 1102 22-23 0.4630950162532841 1102 24-25 0.4813929201747129 1102 26-27 0.42311229485306967 1102 28-29 0.4647683368253013 1102 30-31 0.4744622308839155 1102 32-33 0.45793869167378887 1102 34-35 0.47490316754769424 1102 36-37 0.44086342675686296 1102 38-39 0.5471982200987497 1102 40-41 0.5186115949895225 1102 42-43 0.5393185338555497 1102 44-45 0.48348646089566927 1102 46-47 0.4846343380894709 1102 48-49 0.5353328429666888 1102 50-51 0.5248333083468353 1102 52-53 0.5086214633306128 1102 54-55 0.5316935927687325 1102 56-57 0.5098141375934517 1102 58-59 0.4551152676515571 1102 60-61 0.45574403689084164 1102 62-63 0.5334755612829554 1102 64-65 0.5164587667490466 1102 66-67 0.5480236624922483 1102 68-69 0.5425105022699768 1102 70-71 0.5561904034319909 1102 72-73 0.6658559798981827 1102 74-75 0.565994001396902 1102 76-77 0.5683961781404534 1102 78-79 0.5313642379529071 1102 80-81 0.57080789148851 1102 82-83 0.5448617507038378 1102 84-85 0.507892574095326 1102 86-87 0.5119905938463951 1102 88-89 0.4894228867777528 1102 90-91 0.5209810603503726 1102 92-93 0.3878649814909423 1102 94-95 0.43652429815359994 1102 96-97 0.48181147931246215 1102 98-99 0.5014018663743194 1102 100-101 0.7053286421470979 1103 1 0.26510490078896964 1103 2 0.21042051185863642 1103 3 0.24447292507046825 1103 4 0.19589470446015156 1103 5 0.22354112397295722 1103 6 0.248178643648167 1103 7 0.22318147655427367 1103 8 0.2446058610704469 1103 9 0.2738689555801841 1103 10-11 0.29973201109783787 1103 12-13 0.2581652222164337 1103 14-15 0.40280598932860556 1103 16-17 0.3895288703990971 1103 18-19 0.38443668661864194 1103 20-21 0.43094810787843585 1103 22-23 0.4038817525293936 1103 24-25 0.3473793330054207 1103 26-27 0.29164526106195154 1103 28-29 0.3140851525068058 1103 30-31 0.307655135281351 1103 32-33 0.2765972000228274 1103 34-35 0.2855428414424708 1103 36-37 0.3014913459995583 1103 38-39 0.3429117083738049 1103 40-41 0.29374324448185263 1103 42-43 0.3456077402474236 1103 44-45 0.275735306313031 1103 46-47 0.2990032183159812 1103 48-49 0.3219590597139401 1103 50-51 0.29098748724748447 1103 52-53 0.2795728569292706 1103 54-55 0.30383856749352134 1103 56-57 0.28593604207969747 1103 58-59 0.31528514068452296 1103 60-61 0.33846214856463774 1103 62-63 0.3194675260352753 1103 64-65 0.3028719110928293 1103 66-67 0.31445993267188044 1103 68-69 0.35430690076357507 1103 70-71 0.3294104914907834 1103 72-73 0.3891241919331776 1103 74-75 0.3121606712287601 1103 76-77 0.33008406604532325 1103 78-79 0.30079018763509424 1103 80-81 0.3179942597848644 1103 82-83 0.2809657234385128 1103 84-85 0.30294535555746194 1103 86-87 0.35164442433589826 1103 88-89 0.3731215041784708 1103 90-91 0.3420379153395281 1103 92-93 0.2947883861750249 1103 94-95 0.28111031943792497 1103 96-97 0.3411235600159337 1103 98-99 0.34650084576757223 1103 100-101 0.5802813853295028 1104 1 0.3202973433529337 1104 2 0.2560601810072498 1104 3 0.31018748787460027 1104 4 0.25572079015329763 1104 5 0.29672464258708686 1104 6 0.30442768629040984 1104 7 0.2289816237818485 1104 8 0.26403014898841093 1104 9 0.35566496577375517 1104 10-11 0.40028217590347737 1104 12-13 0.4032149321859393 1104 14-15 0.5154322721303899 1104 16-17 0.5457966901175482 1104 18-19 0.5043697966181213 1104 20-21 0.5590041185192334 1104 22-23 0.5474747694457562 1104 24-25 0.5539925714049474 1104 26-27 0.533367386590534 1104 28-29 0.5832637817925956 1104 30-31 0.5767930048780059 1104 32-33 0.572659911800045 1104 34-35 0.5732595989041656 1104 36-37 0.581880066586514 1104 38-39 0.671391288887051 1104 40-41 0.6812430303911086 1104 42-43 0.6302116481503859 1104 44-45 0.6080352827492064 1104 46-47 0.6568405597290763 1104 48-49 0.6198258664654972 1104 50-51 0.5988150468544049 1104 52-53 0.6131231641934107 1104 54-55 0.6351571580438602 1104 56-57 0.6642884760962673 1104 58-59 0.6422557533052426 1104 60-61 0.6869386278610463 1104 62-63 0.7152100968718145 1104 64-65 0.6849365799200129 1104 66-67 0.6494976648451001 1104 68-69 0.6808286236810588 1104 70-71 0.6909630266044431 1104 72-73 0.7545019629473231 1104 74-75 0.6751903357682281 1104 76-77 0.6976527907343311 1104 78-79 0.6161891976203933 1104 80-81 0.6593145035718528 1104 82-83 0.6206958599910095 1104 84-85 0.6125327118744579 1104 86-87 0.6876885686816152 1104 88-89 0.6548591449495902 1104 90-91 0.585279424334697 1104 92-93 0.6382410114837143 1104 94-95 0.7301776426660957 1104 96-97 0.7732087580223457 1104 98-99 0.8432265847915374 1104 100-101 1.0936693104272877 1105 1 0.24728299627658856 1105 2 0.1754265153649328 1105 3 0.19854585295068716 1105 4 0.18953508488041848 1105 5 0.2071345246665004 1105 6 0.20073214677941564 1105 7 0.2070866384443093 1105 8 0.20512555800861776 1105 9 0.2559561466975282 1105 10-11 0.311715274353098 1105 12-13 0.33021004317521374 1105 14-15 0.3846204136716622 1105 16-17 0.37255307908745294 1105 18-19 0.41934286780382024 1105 20-21 0.46594303805451176 1105 22-23 0.39498101385877504 1105 24-25 0.4026130195548916 1105 26-27 0.40446076715451085 1105 28-29 0.40849247336795713 1105 30-31 0.4568867563182053 1105 32-33 0.43146187367889866 1105 34-35 0.4457726415127041 1105 36-37 0.4713607409461602 1105 38-39 0.5666206407897789 1105 40-41 0.5019949596295348 1105 42-43 0.5895647402695374 1105 44-45 0.4703104791624 1105 46-47 0.49153420993720687 1105 48-49 0.469949253258541 1105 50-51 0.43083480931005624 1105 52-53 0.39093158060509836 1105 54-55 0.43056339546225075 1105 56-57 0.4972930506337434 1105 58-59 0.5163834714582904 1105 60-61 0.5423712338004023 1105 62-63 0.5703101689464702 1105 64-65 0.5264851196624463 1105 66-67 0.5106268013182458 1105 68-69 0.5839242592839469 1105 70-71 0.5860155494891401 1105 72-73 0.617740189139063 1105 74-75 0.5524454987969847 1105 76-77 0.5921324981587119 1105 78-79 0.52460482669904 1105 80-81 0.5274605564199994 1105 82-83 0.5889519229877251 1105 84-85 0.5754620681477469 1105 86-87 0.5980696002116161 1105 88-89 0.5985855241696534 1105 90-91 0.6296123150670176 1105 92-93 0.5759525781091206 1105 94-95 0.5585211139802766 1105 96-97 0.6150226503091005 1105 98-99 0.6066646752226035 1105 100-101 0.8048076138169478 1106 1 0.16174758334531703 1106 2 0.1012030067246883 1106 3 0.13371110760822802 1106 4 0.12380592045530392 1106 5 0.10451159442366986 1106 6 0.12348755533339073 1106 7 0.1487468962386842 1106 8 0.14667205667367256 1106 9 0.19206371717645254 1106 10-11 0.19440754439546737 1106 12-13 0.22422802482924453 1106 14-15 0.22536165748545756 1106 16-17 0.28600005250675764 1106 18-19 0.28210711252100396 1106 20-21 0.34963231131272465 1106 22-23 0.3376959250003111 1106 24-25 0.32169994800166535 1106 26-27 0.29367161113133733 1106 28-29 0.35306260025951985 1106 30-31 0.34313769953272555 1106 32-33 0.33380064201403314 1106 34-35 0.31862412608524693 1106 36-37 0.3264380348443865 1106 38-39 0.42318859499656725 1106 40-41 0.39746776786977733 1106 42-43 0.4076464203779082 1106 44-45 0.35490104757980134 1106 46-47 0.3952604778574198 1106 48-49 0.4273777004971535 1106 50-51 0.38823306830966686 1106 52-53 0.34692309127638765 1106 54-55 0.3886704399437164 1106 56-57 0.3847260911496093 1106 58-59 0.39962769293977374 1106 60-61 0.37519581606981234 1106 62-63 0.43585746136992753 1106 64-65 0.4424609214159432 1106 66-67 0.40937503749471915 1106 68-69 0.44499517517050435 1106 70-71 0.4148458710173202 1106 72-73 0.39812491997316357 1106 74-75 0.4089185220389737 1106 76-77 0.45361208635574 1106 78-79 0.41146311079921816 1106 80-81 0.4363969651165327 1106 82-83 0.4153798066128864 1106 84-85 0.4038728947300534 1106 86-87 0.4251921037052604 1106 88-89 0.42216886305592993 1106 90-91 0.46084642046875146 1106 92-93 0.40978396889196134 1106 94-95 0.342647007924505 1106 96-97 0.4172953397949932 1106 98-99 0.4620938025008243 1106 100-101 0.6797909279938388 1107 1 0.003655071085646 1107 2 -0.006805464133776695 1107 3 0.017244447740026203 1107 4 0.013340161651321125 1107 5 0.015180701533154206 1107 6 0.05889929980754971 1107 7 0.06391506926231472 1107 8 0.07068936696791184 1107 9 0.10330747696178122 1107 10-11 0.1165475522868249 1107 12-13 0.14143565444963002 1107 14-15 0.16849148451596108 1107 16-17 0.1477644664937401 1107 18-19 0.14161012762670566 1107 20-21 0.17815715609420835 1107 22-23 0.1378557791602404 1107 24-25 0.2079908142752771 1107 26-27 0.12567115374100268 1107 28-29 0.18736209673261328 1107 30-31 0.22477361673472274 1107 32-33 0.16964328346805502 1107 34-35 0.177285537469146 1107 36-37 0.1643112257929502 1107 38-39 0.2871029554101199 1107 40-41 0.2175050246143826 1107 42-43 0.27285104458334075 1107 44-45 0.18989852773843552 1107 46-47 0.15535114166655717 1107 48-49 0.13410799621924951 1107 50-51 0.1595358028938918 1107 52-53 0.11941071366678102 1107 54-55 0.1960884760225312 1107 56-57 0.1995467552888215 1107 58-59 0.2125003866664983 1107 60-61 0.21697337766421043 1107 62-63 0.22593689203964828 1107 64-65 0.23607742677214816 1107 66-67 0.22197237749731613 1107 68-69 0.20254702299229166 1107 70-71 0.22142697809805867 1107 72-73 0.2027419897812308 1107 74-75 0.23945620423154423 1107 76-77 0.2685617747510989 1107 78-79 0.22365508466636896 1107 80-81 0.23966251115579595 1107 82-83 0.2951333887272405 1107 84-85 0.28531816812551725 1107 86-87 0.30400062948075046 1107 88-89 0.23274116326137673 1107 90-91 0.3152738747751407 1107 92-93 0.223606933578953 1107 94-95 0.2339596596845226 1107 96-97 0.3162365602063275 1107 98-99 0.28654583662552113 1107 100-101 0.6054375484110395 1108 1 -0.05837279520340388 1108 2 -0.0372406195507935 1108 3 -0.017255656174619105 1108 4 -0.030802479867318766 1108 5 -0.022114375345303472 1108 6 0.003888783297206544 1108 7 -0.0116110059371195 1108 8 0.009699879204717377 1108 9 0.004401679446097262 1108 10-11 0.069265181772316 1108 12-13 0.03149068482892403 1108 14-15 0.020486226076116054 1108 16-17 0.04381076210827928 1108 18-19 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-1.497876077079546 2316 68-69 -1.3972811405044538 2316 70-71 -1.321089649401891 2316 72-73 -1.2586681875492687 2316 74-75 -1.2375156645628635 2316 76-77 -1.2218461331141093 2316 78-79 -1.199562071011627 2316 80-81 -1.244293625332645 2316 82-83 -1.096115184007953 2316 84-85 -1.2841728499086145 2316 86-87 -1.3619500080279856 2316 88-89 -1.531847154620273 2316 90-91 -1.4820296700660798 2316 92-93 -1.5860383606336903 2316 94-95 -1.6466908117011627 2316 96-97 -1.6191572276836617 2316 98-99 -1.617560742777183 2316 100-101 -1.9613770700622233 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 273752.0 3 40279.0 4 35764.0 5 48422.0 6 92382.0 7 183447.0 8 344029.0 9 606311.0 10 772442.0 11 630031.0 12 422121.0 13 286708.0 14 225925.0 15 198472.0 16 189044.0 17 191807.0 18 200919.0 19 217807.0 20 243444.0 21 275288.0 22 317288.0 23 372826.0 24 452206.0 25 487793.0 26 452967.0 27 528661.0 28 625385.0 29 749546.0 30 906943.0 31 1117665.0 32 1396283.0 33 1791368.0 34 2385591.0 35 3362989.0 36 5288397.0 37 1.0455048E7 38 1.8126993E7 39 7559222.0 40 19175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.971623667369183 17.420160566577707 6.871822905535642 46.73639286051747 2 16.60585429088453 16.879321922447524 53.33672921156721 13.178094575100733 3 22.386112561962936 22.01830177045846 31.566836013570704 24.028749654007903 4 23.164145618529048 28.408927283250012 31.61052832283593 16.816398775385014 5 19.033810945948375 32.85640574794768 34.12290456290041 13.986878743203537 6 16.125289431313984 36.68718806995931 34.051549244605624 13.135973254121078 7 13.788035554471865 19.827349181783752 51.28310269362186 15.101512570122516 8 13.094315763255409 25.56297826716176 45.21314745772892 16.1295585118539 9 15.724847720345696 21.4389673279496 45.05999792785501 17.776187023849687 10-11 20.407068032931218 31.44709796215304 31.59558503480592 16.55024897010982 12-13 18.05580455590213 27.545441160352514 37.19661548243758 17.202138801307772 14-15 17.741442185082867 28.742736276591668 37.54689917385334 15.968922364472121 16-17 17.89391860901237 29.15491893378804 36.23240069746776 16.71876175973183 18-19 18.402487278366998 30.21033595661457 33.975171057214006 17.41200570780443 20-21 18.680785145697463 29.13441523966447 34.234003696791255 17.95079591784681 22-23 20.24498216949595 29.315235440554112 34.81610749059321 15.623674899356729 24-25 17.72385648059244 31.52071416316864 34.355155838615666 16.40027351762326 26-27 17.631929496892145 30.635639409617166 34.433332591791185 17.299098501699508 28-29 18.68368359738427 31.035748166716576 31.499026682270838 18.781541553628323 30-31 19.02532334406141 28.85531332413483 33.01920975651678 19.100153575286978 32-33 19.610010177414818 30.39745444780884 32.55626973453208 17.436265640244258 34-35 17.148051698131493 33.14409622501231 30.955885246157983 18.75196683069822 36-37 16.86047522396839 30.791042746318258 33.44442495208232 18.904057077631027 38-39 18.682799487314792 29.215707334621914 32.683081343535164 19.418411834528133 40-41 20.408035667084864 29.282644452268492 31.514894194400057 18.794425686246587 42-43 19.114415660457894 31.5501215830793 31.422294994755497 17.913167761707317 44-45 19.3942290846361 30.419587183884207 32.184633436147806 18.001550295331892 46-47 18.137243167644314 29.863129899332392 32.05020064493051 19.949426288092788 48-49 19.194869595935852 30.286742965757522 30.13161863617294 20.38676880213368 50-51 19.038838576936865 29.447664227989957 31.653318255132984 19.860178939940194 52-53 18.275557094261686 30.385120605893402 33.50040600815282 17.838916291692094 54-55 18.756196926319685 31.217064381059412 31.583855671683423 18.442883020937476 56-57 19.450324475254604 30.37691475881028 31.69528366867695 18.47747709725817 58-59 19.45176005927286 29.59433931648819 30.655823570411684 20.29807705382727 60-61 20.305728305258164 30.195029748396866 30.95632263749581 18.54291930884916 62-63 18.110829579478512 33.89426255760564 30.204239754587974 17.79066810832787 64-65 17.47224950699757 32.55340534765079 32.06462265542324 17.9097224899284 66-67 18.042609517128696 31.06093954745769 32.50968998054279 18.386760954870823 68-69 20.781539058701444 30.671917719491876 29.588005864898896 18.958537356907783 70-71 20.076654151959257 31.114820774250713 29.510941483010445 19.29758359077959 72-73 18.720227384364094 32.86734422867209 30.26328257498243 18.149145811981384 74-75 19.023619843081836 31.15435583560083 31.806970591746648 18.01505372957069 76-77 18.725654336108096 32.04379028336203 30.43886648060674 18.791688899923134 78-79 18.68455465582323 32.329565950779546 29.38473764007586 19.601141753321368 80-81 19.615075427894606 30.482930241910566 32.21453621856566 17.687458111629166 82-83 18.532887330532553 32.78131839757019 31.006638322061008 17.679155949836247 84-85 18.06816041957119 32.70765894530857 30.76412454626436 18.46005608885588 86-87 19.78946052074237 31.826587286601082 29.840489151681094 18.543463040975457 88-89 19.75712239779115 30.5234832185404 31.185564719450483 18.533829664217972 90-91 20.39685471392141 31.034800602812123 30.485781271777384 18.082563411489083 92-93 18.447141760588885 33.17496637101243 30.173384815108335 18.20450705329035 94-95 17.66561275071739 32.84661372440388 30.762585300824423 18.72518822405431 96-97 18.362402417643526 30.553334239469944 32.331629898552684 18.752633444333853 98-99 18.96904074372608 31.478388263106233 30.62850231851196 18.924068674655732 100-101 20.13877901739006 32.0817009673814 29.09235797786743 18.68716203736111 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 73005.0 1 42143.5 2 11050.0 3 12132.5 4 15216.0 5 19558.5 6 24810.5 7 31084.0 8 38319.5 9 46222.5 10 54807.0 11 63640.0 12 72766.0 13 82757.0 14 94152.0 15 106889.0 16 121393.5 17 138948.0 18 160956.5 19 188515.5 20 225014.0 21 272744.5 22 331465.5 23 404407.5 24 494516.0 25 607651.5 26 733382.5 27 886200.0 28 1099427.5 29 1332113.0 30 1587877.0 31 1942416.0 32 2475471.5 33 3018732.5 34 3480886.5 35 4046086.0 36 4464965.5 37 4367984.0 38 3799006.0 39 3054807.0 40 2507417.5 41 2168067.0 42 1913443.0 43 1750933.5 44 1609298.0 45 1483869.5 46 1386737.5 47 1282856.5 48 1176137.5 49 1071848.5 50 954492.5 51 833719.0 52 716287.5 53 617272.5 54 526058.5 55 435040.5 56 356459.0 57 285247.5 58 222280.5 59 168046.0 60 121539.0 61 86512.0 62 62241.5 63 43568.5 64 28552.5 65 17742.5 66 11413.0 67 7973.5 68 5598.0 69 3889.0 70 3373.0 71 4767.0 72 4483.5 73 4886.0 74 5035.0 75 2740.0 76 2056.0 77 1750.0 78 1462.5 79 957.5 80 562.5 81 318.0 82 215.5 83 146.5 84 94.5 85 63.0 86 45.0 87 48.0 88 44.0 89 31.0 90 19.5 91 16.0 92 16.5 93 12.5 94 14.0 95 10.0 96 3.5 97 4.5 98 3.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.367540938353842 2 0.3104982744169915 3 0.28340159489963107 4 0.2849757429283743 5 0.2816141126411198 6 0.21840091772506845 7 0.23163087230750382 8 0.2294700551468984 9 0.22886237582574082 10-11 0.23019086625656934 12-13 0.23815938458892918 14-15 0.23301108012736702 16-17 0.24338930555506175 18-19 0.2449012311001226 20-21 0.23389108382516033 22-23 0.20918067696122844 24-25 0.20226994085146863 26-27 0.18030297985898608 28-29 0.1764775092388267 30-31 0.1976436587854753 32-33 0.21039280326672888 34-35 0.21077987560028533 36-37 0.20510389215372865 38-39 0.1994311410439866 40-41 0.1835886502310959 42-43 0.1787393369248905 44-45 0.16900919502853667 46-47 0.17944560251889544 48-49 0.20136488654336165 50-51 0.2057673292849392 52-53 0.23146925546677044 54-55 0.2417513188742288 56-57 0.24442122908314443 58-59 0.222844572761033 60-61 0.2129285714978358 62-63 0.2052396502999447 64-65 0.19922427148784788 66-67 0.20348529949378372 68-69 0.20422065611912069 70-71 0.2168631334854902 72-73 0.24840104378620417 74-75 0.25697724143972156 76-77 0.23548139353797687 78-79 0.21641787908926968 80-81 0.20572530890634855 82-83 0.19244363693487843 84-85 0.1801874238178617 86-87 0.2018529694023765 88-89 0.1773922605573777 90-91 0.17170334776356233 92-93 0.21854071629230282 94-95 0.2184332410932151 96-97 0.22051082558084287 98-99 0.20969623468316798 100-101 0.1890739258055872 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6.187474E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.42839440624197 #Duplication Level Percentage of deduplicated Percentage of total 1 78.75523549252878 35.77723899511064 2 10.722365145596717 9.741996656038197 3 3.7994449319350454 5.178080486782274 4 1.8817092813799345 3.4193212556965524 5 1.1139475247455575 2.530242375099909 6 0.7348797901884971 2.003064536991564 7 0.512179641301234 1.6287249126315997 8 0.37885395505805625 1.376858151419364 9 0.2866721013284838 1.1720747955974875 >10 1.601363066143133 13.264679989982394 >50 0.12365898300589963 3.874275069671957 >100 0.07712182883949259 6.753404983014331 >500 0.006866240148921201 2.145875155341912 >1k 0.004741187435265818 4.187548123515953 >5k 5.357832938328275E-4 1.6920635714811578 >10k+ 4.250470711521277E-4 5.254550941624708 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 393079 0.6352818613864075 Illumina Single End PCR Primer 1 (100% over 50bp) CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 353832 0.5718520999037733 No Hit AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA 86351 0.13955775814169077 No Hit GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA 75283 0.12167000620931902 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 65956 0.1065960034741156 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.7520855845212441 0.0 0.0 1.9394020888006962E-5 0.0 2 0.7574997486858127 1.6161684073339137E-6 0.0 2.585869451734262E-5 0.0 3 0.7575433852328107 1.6161684073339137E-6 0.0 3.3939536554012187E-5 0.0 4 0.7577292445996541 1.6161684073339137E-6 0.0 4.363654699801567E-5 0.0 5 0.7578520733986115 1.6161684073339137E-6 0.0 5.171738903468524E-5 1.6161684073339137E-6 6 0.7579991447236788 1.6161684073339137E-6 0.0 6.626290470069046E-5 1.6161684073339137E-6 7 0.7580961148281189 1.6161684073339137E-6 0.0 6.949524151535829E-5 1.6161684073339137E-6 8 0.7582722771845183 1.6161684073339137E-6 0.0 7.919225195936176E-5 1.6161684073339137E-6 9 0.7585454096453577 1.6161684073339137E-6 0.0 8.404075718136351E-5 1.6161684073339137E-6 10-11 0.7590197550729103 1.6161684073339137E-6 0.0 1.010105254583696E-4 1.6161684073339137E-6 12-13 0.7598116775925039 1.6161684073339137E-6 0.0 1.2282879895737743E-4 3.2323368146678273E-6 14-15 0.7609413793092302 1.6161684073339137E-6 0.0 1.406066514380505E-4 3.2323368146678273E-6 16-17 0.7623522943288328 1.6161684073339137E-6 0.0 1.5353599869672178E-4 3.2323368146678273E-6 18-19 0.7642609892178941 1.6161684073339137E-6 0.0 1.6808151436272702E-4 3.2323368146678273E-6 20-21 0.7665599887773266 3.2323368146678273E-6 0.0 1.8989978786173483E-4 3.2323368146678273E-6 22-23 0.7690820195769712 3.2323368146678273E-6 0.0 2.1010189295340878E-4 3.2323368146678273E-6 24-25 0.771484453914473 3.2323368146678273E-6 0.0 2.2787974543408182E-4 3.2323368146678273E-6 26-27 0.7738295142735145 3.2323368146678273E-6 0.0 2.4000100848908616E-4 4.8485052220017406E-6 28-29 0.7767103344595873 4.848505222001741E-6 0.0 2.707082082284305E-4 4.8485052220017406E-6 30-31 0.77973660980232 6.464673629335655E-6 0.0 2.9737498694944013E-4 4.8485052220017406E-6 32-33 0.7831370281313506 8.080842036669568E-6 0.0 3.240417656704497E-4 6.464673629335655E-6 34-35 0.7866360327332285 1.1313178851337396E-5 0.0 3.353549445217871E-4 6.464673629335655E-6 36-37 0.7902667550603041 1.2121263055004352E-5 0.0 3.458600391694575E-4 6.464673629335655E-6 38-39 0.7946433391073644 1.292934725867131E-5 0.0 3.579813022244619E-4 6.464673629335655E-6 40-41 0.801294680187747 1.292934725867131E-5 0.0 3.8060765992713663E-4 6.464673629335655E-6 42-43 0.8090660259744121 1.292934725867131E-5 0.0 3.9757742820414275E-4 6.464673629335655E-6 44-45 0.8152187791011324 1.292934725867131E-5 0.0 4.056582702408123E-4 6.464673629335655E-6 46-47 0.8224010315033243 1.6161684073339136E-5 0.0 4.137391122774819E-4 8.080842036669568E-6 48-49 0.8306168236020063 1.6161684073339136E-5 0.0 4.2586037533248625E-4 8.080842036669568E-6 50-51 0.8401126210792966 1.6161684073339136E-5 0.0 4.3394121736915585E-4 8.080842036669568E-6 52-53 0.8497304392713407 1.6969768277006093E-5 0.0 4.452543962204932E-4 8.080842036669568E-6 54-55 0.8596399758609086 1.777785248067305E-5 0.0 4.646484171085002E-4 8.080842036669568E-6 56-57 0.8734331651332999 1.777785248067305E-5 0.0 4.7919393277450536E-4 8.080842036669568E-6 58-59 0.8877120130120951 1.8585936684340008E-5 0.0 5.123253851248506E-4 8.080842036669568E-6 60-61 0.9014421717166003 2.1010189295340877E-5 0.0 5.309113218091906E-4 8.080842036669568E-6 62-63 0.918165474311488 2.1818273499007835E-5 0.0 5.389921638458602E-4 8.080842036669568E-6 64-65 0.9345114015832632 2.2626357702674792E-5 0.0 5.470730058825297E-4 8.080842036669568E-6 66-67 0.9576169532187125 2.2626357702674792E-5 0.0 5.624266057522019E-4 8.080842036669568E-6 68-69 0.9793431051185023 2.2626357702674792E-5 0.0 5.696993635852046E-4 8.080842036669568E-6 70-71 1.0090660582977804 2.4242526110008704E-5 0.0 5.785882898255411E-4 8.080842036669568E-6 72-73 1.039961541656579 2.7474862924676534E-5 0.0 5.971742265098812E-4 8.888926240336525E-6 74-75 1.071053389476869 2.7474862924676534E-5 0.0 6.157601631942211E-4 9.697010444003481E-6 76-77 1.1065678821438278 3.070719973934436E-5 0.0 6.408107735078967E-4 9.697010444003481E-6 78-79 1.1458827948206327 3.232336814667827E-5 0.0 6.585886259885699E-4 1.0505094647670439E-5 80-81 1.2025949523181834 3.3939536554012187E-5 0.0 6.804068994875777E-4 1.1313178851337396E-5 82-83 1.2546323426975208 3.3939536554012187E-5 0.0 7.022251729865855E-4 1.1313178851337396E-5 84-85 1.316135631438613 3.55557049613461E-5 0.0 7.183868570599247E-4 1.1313178851337396E-5 86-87 1.3993658801636983 3.7171873368680016E-5 0.0 7.369727937442647E-4 1.1313178851337396E-5 88-89 1.506109763047085 3.7171873368680016E-5 0.0 7.531344778176038E-4 1.1313178851337396E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 41490 0.0 64.48735 1 GCGTGTT 50920 0.0 52.548546 2 CTGGAAT 300725 0.0 46.856052 1 CGTGTTT 121535 0.0 46.166325 1 AGAGCGT 100425 0.0 44.88208 9 AAGAGCG 107500 0.0 42.20675 8 GTCGCCG 58765 0.0 41.755363 44-45 CCGTATC 60590 0.0 41.186916 48-49 CGCCGTA 60900 0.0 40.856133 46-47 TGGTCGC 59510 0.0 40.007935 42-43 TCGGTGG 65700 0.0 38.075676 38-39 CGTATCA 60010 0.0 37.757687 50-51 GGTCGCC 60260 0.0 37.48018 44-45 CGGAAGA 121265 0.0 37.477345 5 TCGCCGT 59565 0.0 37.417183 46-47 TCTCGGT 65780 0.0 37.36842 36-37 GTGGTCG 59985 0.0 37.102573 42-43 GATCTCG 69790 0.0 36.956585 34-35 TCGGAAG 125465 0.0 36.32053 4 CGGCGAG 5435 0.0 36.138386 1 >>END_MODULE