FastQCFastQC Report
Fri 27 May 2016
SRR1233064_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233064_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61874740
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG4133750.6680836153816565TruSeq Adapter, Index 5 (100% over 49bp)
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC3572450.577368082678004No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1100290.17782539369054318TruSeq Adapter, Index 5 (100% over 50bp)
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA937020.15143821210400238No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA753480.12177505715579573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT408350.068.950171
GCGTGTT503650.055.641162
CTGGAAT3010500.047.5168761
CGTGTTT1210750.047.1898351
TGCCGTC714950.040.9129848-49
TATGCCG731650.040.1414346-47
CCGTCTT735850.039.81548750-51
CTCGTAT721000.039.50262542-43
CGTATGC752400.039.1071344-45
ATCTCGT754400.037.38450240-41
CGGAAGA1307300.035.722135
AGATCGG1295900.035.4498141
TCGGAAG1338300.034.9146274
ATCGGAA1352650.034.167813
GATCGGA1371150.033.850572
AGTGATC856100.032.77229736-37
GTGTTTT3203300.032.388611
GCCGTCT718050.032.22728750-51
ATGCCGT722050.032.0750648-49
TCTCGTA705450.031.60050242-43
Produced by FastQC (version 0.11.5)