FastQCFastQC Report
Fri 27 May 2016
SRR1233063_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233063_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73939087
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC8883831.2015065860902503Illumina Single End PCR Primer 1 (100% over 50bp)
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1782900.24113091902257328No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG921480.12462691079753256No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA842220.11390727613393441No Hit
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA838950.11346502019966788No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT833430.1127184597234748No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN787670.10652958157300481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT91200.056.8119132
CGCGTGT486250.054.7401431
TCCGATC95600.054.023431
GCGTGTT613700.041.632272
AGAGCGT2532950.040.277959
CCGTATC1371000.040.20896548-49
CGCCGTA1372200.040.0760146-47
GTCGCCG1370600.039.6920144-45
CTGGAAT1959100.038.705991
TGGTCGC1387350.038.1140442-43
CGTATCA1358750.037.88260350-51
AAGAGCG2719700.037.7955138
TCGCCGT1350400.037.4073746-47
TCGGTGG1496250.036.7785938-39
GGTCGCC1394500.036.70430844-45
GTTTTTT9735950.036.2215041
GTGGTCG1394050.036.11870642-43
GCCGTAT1428100.036.06978248-49
TCTCGGT1535050.035.3385136-37
GGTGGTC1521300.034.8282340-41