Basic Statistics
Measure | Value |
---|---|
Filename | SRR1233063_2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 73939087 |
Sequences flagged as poor quality | 0 |
Sequence length | 101 |
%GC | 38 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 888383 | 1.2015065860902503 | Illumina Single End PCR Primer 1 (100% over 50bp) |
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC | 178290 | 0.24113091902257328 | No Hit |
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG | 92148 | 0.12462691079753256 | No Hit |
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA | 84222 | 0.11390727613393441 | No Hit |
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA | 83895 | 0.11346502019966788 | No Hit |
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT | 83343 | 0.1127184597234748 | No Hit |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | 78767 | 0.10652958157300481 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCGATCT | 9120 | 0.0 | 56.811913 | 2 |
CGCGTGT | 48625 | 0.0 | 54.740143 | 1 |
TCCGATC | 9560 | 0.0 | 54.02343 | 1 |
GCGTGTT | 61370 | 0.0 | 41.63227 | 2 |
AGAGCGT | 253295 | 0.0 | 40.27795 | 9 |
CCGTATC | 137100 | 0.0 | 40.208965 | 48-49 |
CGCCGTA | 137220 | 0.0 | 40.07601 | 46-47 |
GTCGCCG | 137060 | 0.0 | 39.69201 | 44-45 |
CTGGAAT | 195910 | 0.0 | 38.70599 | 1 |
TGGTCGC | 138735 | 0.0 | 38.11404 | 42-43 |
CGTATCA | 135875 | 0.0 | 37.882603 | 50-51 |
AAGAGCG | 271970 | 0.0 | 37.795513 | 8 |
TCGCCGT | 135040 | 0.0 | 37.40737 | 46-47 |
TCGGTGG | 149625 | 0.0 | 36.77859 | 38-39 |
GGTCGCC | 139450 | 0.0 | 36.704308 | 44-45 |
GTTTTTT | 973595 | 0.0 | 36.221504 | 1 |
GTGGTCG | 139405 | 0.0 | 36.118706 | 42-43 |
GCCGTAT | 142810 | 0.0 | 36.069782 | 48-49 |
TCTCGGT | 153505 | 0.0 | 35.33851 | 36-37 |
GGTGGTC | 152130 | 0.0 | 34.82823 | 40-41 |