##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233063_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 73939087 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.980615381415245 34.0 31.0 34.0 30.0 34.0 2 32.23008682809405 34.0 31.0 34.0 30.0 34.0 3 32.21304141881005 34.0 31.0 34.0 30.0 34.0 4 35.59585764157461 37.0 35.0 37.0 33.0 37.0 5 35.57000726828017 37.0 35.0 37.0 33.0 37.0 6 35.59959128248365 37.0 35.0 37.0 33.0 37.0 7 35.670888944030374 37.0 35.0 37.0 35.0 37.0 8 35.64532932358226 37.0 35.0 37.0 33.0 37.0 9 37.325230009940476 39.0 38.0 39.0 34.0 39.0 10-11 37.19320555040124 39.0 37.0 39.0 34.0 39.0 12-13 37.125992406425034 39.0 37.0 39.0 34.0 39.0 14-15 38.42893901300134 40.0 38.0 41.0 34.0 41.0 16-17 38.23575977479949 40.0 38.0 41.0 33.0 41.0 18-19 37.94470212351959 40.0 38.0 41.0 33.0 41.0 20-21 37.68931786377075 40.0 38.0 41.0 32.5 41.0 22-23 37.443939509288235 40.0 38.0 41.0 32.0 41.0 24-25 37.10343857775793 40.0 37.0 41.0 31.5 41.0 26-27 36.96638413996105 40.0 38.0 41.0 31.0 41.0 28-29 36.70981409332252 40.0 37.5 41.0 30.0 41.0 30-31 36.471414828262624 40.0 37.0 41.0 29.0 41.0 32-33 36.35705006068035 40.0 37.0 41.0 29.0 41.0 34-35 36.27896175537033 40.0 37.0 41.0 28.5 41.0 36-37 36.18794670131645 40.0 37.0 41.0 27.5 41.0 38-39 36.02725367030837 40.0 37.0 41.0 27.0 41.0 40-41 35.9289416300204 40.0 37.0 41.0 26.5 41.0 42-43 35.735767078108495 40.0 36.5 41.0 25.0 41.0 44-45 35.64910230768741 40.0 36.0 41.0 25.0 41.0 46-47 35.674482733334266 40.0 36.5 41.0 25.0 41.0 48-49 35.700566582597915 40.0 36.5 41.0 25.0 41.0 50-51 35.32159962294368 39.5 35.5 40.5 24.5 41.0 52-53 35.327279473981065 39.5 36.0 40.5 24.0 41.0 54-55 35.53279272301536 40.0 36.0 41.0 25.0 41.0 56-57 35.44575290062751 40.0 36.0 41.0 25.0 41.0 58-59 35.281556600773285 40.0 35.5 41.0 24.0 41.0 60-61 35.06422095122706 39.0 35.0 41.0 23.0 41.0 62-63 34.81046143428847 39.0 35.0 41.0 22.5 41.0 64-65 34.48574743017858 39.0 35.0 41.0 21.0 41.0 66-67 33.96109345385885 38.5 34.0 40.5 14.0 41.0 68-69 33.61143802735893 38.0 34.0 40.0 9.0 41.0 70-71 33.26544521032563 37.0 34.0 40.0 8.0 41.0 72-73 32.860258397564465 37.0 33.5 39.5 7.0 41.0 74-75 32.49982557263657 36.0 33.0 39.0 6.0 41.0 76-77 32.062618754002195 36.0 32.5 39.0 2.0 40.5 78-79 31.6968896370062 35.0 32.0 37.5 2.0 39.5 80-81 31.306949901883428 35.0 32.0 37.0 2.0 39.0 82-83 30.880134488271406 35.0 32.0 37.0 2.0 39.0 84-85 30.524471765522343 35.0 31.0 36.0 2.0 38.0 86-87 30.165177262737906 35.0 31.0 36.0 2.0 37.0 88-89 29.791334926816177 35.0 31.0 35.5 2.0 37.0 90-91 29.49596317709468 35.0 31.0 35.0 2.0 36.5 92-93 29.218982106176128 34.5 30.5 35.0 2.0 36.0 94-95 28.950110785111534 34.0 30.0 35.0 2.0 36.0 96-97 28.636220724499886 34.0 30.0 35.0 2.0 36.0 98-99 28.235713473172858 34.0 29.5 35.0 2.0 35.0 100-101 26.53266368436494 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28481508210833084 1101 2 0.2581754405737158 1101 3 0.27304713302141437 1101 4 0.22760086315682315 1101 5 0.21540232101120438 1101 6 0.22859503755783805 1101 7 0.22497377741478886 1101 8 0.2326274687992793 1101 9 0.28646229920379085 1101 10-11 0.3407066182487455 1101 12-13 0.35827576797672833 1101 14-15 0.5127051092132646 1101 16-17 0.5128081309303454 1101 18-19 0.5197119312739247 1101 20-21 0.5821008427366863 1101 22-23 0.5729492353503574 1101 24-25 0.5908969231204324 1101 26-27 0.5229787139923374 1101 28-29 0.5869524928742251 1101 30-31 0.5517250099719959 1101 32-33 0.5309777168118046 1101 34-35 0.5385942244564106 1101 36-37 0.561002566035377 1101 38-39 0.6653917824839937 1101 40-41 0.5599526660587699 1101 42-43 0.5989285221822769 1101 44-45 0.5357880431646649 1101 46-47 0.5453300644643235 1101 48-49 0.5731851818518052 1101 50-51 0.5234693601580602 1101 52-53 0.5153727422405368 1101 54-55 0.5802509041664905 1101 56-57 0.55965816287965 1101 58-59 0.5680336264900774 1101 60-61 0.539499004279115 1101 62-63 0.6408536252720793 1101 64-65 0.5098866187941269 1101 66-67 0.5491476101594799 1101 68-69 0.6455782930304466 1101 70-71 0.628858729406339 1101 72-73 0.6687871736565185 1101 74-75 0.5661215068991012 1101 76-77 0.5242549686962334 1101 78-79 0.5721561666381767 1101 80-81 0.5741352879964765 1101 82-83 0.5880933797091181 1101 84-85 0.636073320230043 1101 86-87 0.6528353563111224 1101 88-89 0.5799674494540383 1101 90-91 0.5961349213473461 1101 92-93 0.5940685418520708 1101 94-95 0.5824934264606512 1101 96-97 0.6072416204606448 1101 98-99 0.6603630775160276 1101 100-101 0.8964362914280244 1102 1 0.23136032189706413 1102 2 0.21447006750882736 1102 3 0.25848725305399256 1102 4 0.21019078923824708 1102 5 0.21190605543535668 1102 6 0.2306456717792642 1102 7 0.2066360463594208 1102 8 0.23452300348452582 1102 9 0.3104732746413532 1102 10-11 0.340210409009039 1102 12-13 0.3407150902585201 1102 14-15 0.4353355109206589 1102 16-17 0.4685434893039968 1102 18-19 0.49836274485446097 1102 20-21 0.5159089755855675 1102 22-23 0.48489598860685845 1102 24-25 0.5133599491421208 1102 26-27 0.4618376862501279 1102 28-29 0.49566090758643355 1102 30-31 0.49855169743436534 1102 32-33 0.5253463017856319 1102 34-35 0.5003470674120649 1102 36-37 0.4918977653397434 1102 38-39 0.5889465673093781 1102 40-41 0.5766341780702859 1102 42-43 0.5867710846073066 1102 44-45 0.5321006805426336 1102 46-47 0.49220867581880157 1102 48-49 0.572519174095639 1102 50-51 0.5143569575999933 1102 52-53 0.477996058908694 1102 54-55 0.49868427806482885 1102 56-57 0.4873625452270147 1102 58-59 0.47689213272595055 1102 60-61 0.4701146636226312 1102 62-63 0.5551984893117279 1102 64-65 0.4866818406478757 1102 66-67 0.5005845647956377 1102 68-69 0.525881199382205 1102 70-71 0.5167872866922565 1102 72-73 0.5773917839254707 1102 74-75 0.499294556477885 1102 76-77 0.4930621221459859 1102 78-79 0.45637204875991344 1102 80-81 0.47885895679431556 1102 82-83 0.45191221024395034 1102 84-85 0.3974129106765787 1102 86-87 0.40710401287356035 1102 88-89 0.3843718793919102 1102 90-91 0.41993731947659896 1102 92-93 0.2963630236873307 1102 94-95 0.3606444896318308 1102 96-97 0.3960424259516877 1102 98-99 0.4486390051427911 1102 100-101 0.6306774836051368 1103 1 0.22879031917155146 1103 2 0.1831289400997136 1103 3 0.2170798438817485 1103 4 0.15973057344797326 1103 5 0.18965055828680022 1103 6 0.20025895905141056 1103 7 0.19583957013832531 1103 8 0.21466639702034485 1103 9 0.2700669762032675 1103 10-11 0.2892645022710383 1103 12-13 0.24095843884300194 1103 14-15 0.3878874244517121 1103 16-17 0.3758824998308512 1103 18-19 0.3544151941695475 1103 20-21 0.40046304553438716 1103 22-23 0.38651582749672997 1103 24-25 0.33793202685435375 1103 26-27 0.2820319869676595 1103 28-29 0.3167735724380236 1103 30-31 0.2916947059301691 1103 32-33 0.2580325609470506 1103 34-35 0.2654260826975161 1103 36-37 0.2755191966792907 1103 38-39 0.34085573973551675 1103 40-41 0.2616570663641724 1103 42-43 0.3042932272426242 1103 44-45 0.2315382481395858 1103 46-47 0.2550439265510889 1103 48-49 0.27202634289248095 1103 50-51 0.27145926244952534 1103 52-53 0.2402345842924163 1103 54-55 0.28633345105740915 1103 56-57 0.2457517431005627 1103 58-59 0.2796133891831829 1103 60-61 0.27249096162405095 1103 62-63 0.2975985922536353 1103 64-65 0.2696713351597353 1103 66-67 0.2806072751941002 1103 68-69 0.33795527394384095 1103 70-71 0.31437383357560833 1103 72-73 0.3605473008380642 1103 74-75 0.28746810455510285 1103 76-77 0.29518813027870294 1103 78-79 0.26675333007970536 1103 80-81 0.28309738776373905 1103 82-83 0.2528439683555668 1103 84-85 0.3069146558980229 1103 86-87 0.3600494175705222 1103 88-89 0.3639162598431298 1103 90-91 0.3247245414785205 1103 92-93 0.2593502156906524 1103 94-95 0.2519122054788596 1103 96-97 0.3170139807534902 1103 98-99 0.33269440671671546 1103 100-101 0.5846682698999182 1104 1 0.3050923151886664 1104 2 0.25745614767902936 1104 3 0.3003410782056761 1104 4 0.24245002876244826 1104 5 0.2671037811622057 1104 6 0.297727623143885 1104 7 0.24314487371903937 1104 8 0.28261174894585395 1104 9 0.33942851863749723 1104 10-11 0.3891254682109846 1104 12-13 0.3885271645489681 1104 14-15 0.5186946395403638 1104 16-17 0.5580838713552794 1104 18-19 0.521678358635576 1104 20-21 0.5525581234609049 1104 22-23 0.552194603056094 1104 24-25 0.5551718547975213 1104 26-27 0.5025450965534546 1104 28-29 0.5247885517128239 1104 30-31 0.5429682635349025 1104 32-33 0.550301503204679 1104 34-35 0.5559930960089261 1104 36-37 0.5649438652217569 1104 38-39 0.6837396697294338 1104 40-41 0.6650041198738208 1104 42-43 0.6390912654616159 1104 44-45 0.6202105608839474 1104 46-47 0.6465300915617647 1104 48-49 0.592039648158611 1104 50-51 0.6009955176392552 1104 52-53 0.6232163645623885 1104 54-55 0.6305176928342533 1104 56-57 0.624542802536979 1104 58-59 0.6179967641644595 1104 60-61 0.6607568388933132 1104 62-63 0.6912898668618013 1104 64-65 0.6729994190762127 1104 66-67 0.6083340117138363 1104 68-69 0.6381276252915811 1104 70-71 0.6371709828963859 1104 72-73 0.6999270968442559 1104 74-75 0.6245535016232466 1104 76-77 0.6653740813449573 1104 78-79 0.5752331452781725 1104 80-81 0.5979471081989267 1104 82-83 0.5690199575554828 1104 84-85 0.5666360868073355 1104 86-87 0.6472983615253973 1104 88-89 0.6012134106729761 1104 90-91 0.5381942085762041 1104 92-93 0.6317024554838966 1104 94-95 0.6671303646507312 1104 96-97 0.7084712710268803 1104 98-99 0.7955229095954301 1104 100-101 1.0494670708175242 1105 1 0.28616842388370856 1105 2 0.19590495240321815 1105 3 0.2132854573809695 1105 4 0.20937225043451235 1105 5 0.2163879825038748 1105 6 0.20513412766724315 1105 7 0.221992078945199 1105 8 0.2332279226499807 1105 9 0.2779732229367795 1105 10-11 0.32239019926669243 1105 12-13 0.3483439164799407 1105 14-15 0.4119939196647948 1105 16-17 0.41053913161701416 1105 18-19 0.43980979985642676 1105 20-21 0.5197384466000372 1105 22-23 0.4549352774440294 1105 24-25 0.4794607292964699 1105 26-27 0.43429490869474563 1105 28-29 0.4328658323947039 1105 30-31 0.46221743781040914 1105 32-33 0.4194331165474807 1105 34-35 0.4637146187044152 1105 36-37 0.4789180259236403 1105 38-39 0.6177719982178473 1105 40-41 0.5663802526973711 1105 42-43 0.6312562094441034 1105 44-45 0.5088788968262605 1105 46-47 0.5197395181713276 1105 48-49 0.5201982352039138 1105 50-51 0.4708917696892385 1105 52-53 0.41702822691541996 1105 54-55 0.44166294253646043 1105 56-57 0.524020692511975 1105 58-59 0.5449713101222784 1105 60-61 0.5690553352162695 1105 62-63 0.5847485655612985 1105 64-65 0.5320722576686236 1105 66-67 0.4835862478880131 1105 68-69 0.5388913201503058 1105 70-71 0.5401807827011993 1105 72-73 0.5920197160279912 1105 74-75 0.5365873203347533 1105 76-77 0.5570703924265459 1105 78-79 0.513599119977453 1105 80-81 0.5035044950062435 1105 82-83 0.5837610733586835 1105 84-85 0.5617170659318518 1105 86-87 0.5572390223236283 1105 88-89 0.5588816523733442 1105 90-91 0.6106402325556708 1105 92-93 0.5841433011578196 1105 94-95 0.5303339195369468 1105 96-97 0.5824635181085434 1105 98-99 0.5696414140907855 1105 100-101 0.7609996334818057 1106 1 0.16095893856463306 1106 2 0.09981374247654173 1106 3 0.1254076801615298 1106 4 0.10953850009378385 1106 5 0.07712805119871291 1106 6 0.1255171875218153 1106 7 0.13096879652938753 1106 8 0.1298375537757508 1106 9 0.19394969492313407 1106 10-11 0.20114254734842518 1106 12-13 0.23691177071732739 1106 14-15 0.23862675561651514 1106 16-17 0.3009866079859904 1106 18-19 0.301918352352736 1106 20-21 0.33930045398550845 1106 22-23 0.30116296981986324 1106 24-25 0.2855617672372617 1106 26-27 0.22164424648540404 1106 28-29 0.27410334779004586 1106 30-31 0.2660563224570325 1106 32-33 0.24297359567470522 1106 34-35 0.2239366982597062 1106 36-37 0.24415750778403122 1106 38-39 0.3487695816600933 1106 40-41 0.31360214417164656 1106 42-43 0.3249161718062368 1106 44-45 0.2653459509291025 1106 46-47 0.27962345050965354 1106 48-49 0.33429233066589603 1106 50-51 0.3135373927109626 1106 52-53 0.26313356967927604 1106 54-55 0.28475689700339757 1106 56-57 0.2863143999279103 1106 58-59 0.3054864868264815 1106 60-61 0.25576514611519485 1106 62-63 0.336531121818858 1106 64-65 0.3057782294834297 1106 66-67 0.25021638643801936 1106 68-69 0.29942730795068684 1106 70-71 0.28240911095848986 1106 72-73 0.27370575991881907 1106 74-75 0.325576461024248 1106 76-77 0.36376204168281134 1106 78-79 0.3279270285292206 1106 80-81 0.33803355046423533 1106 82-83 0.3250334593981421 1106 84-85 0.292264171195427 1106 86-87 0.3086357988229018 1106 88-89 0.3305185963607258 1106 90-91 0.3446439849860319 1106 92-93 0.2780992611143809 1106 94-95 0.22675846861172744 1106 96-97 0.30358734958277367 1106 98-99 0.3317100170088274 1106 100-101 0.5648633584506761 1107 1 -0.0034403192934107096 1107 2 -0.004357747908322551 1107 3 0.027096858464155105 1107 4 0.021676804627993818 1107 5 0.0093555142878472 1107 6 0.05446222136613699 1107 7 0.04953718997336409 1107 8 0.04980488166886232 1107 9 0.10884031606811817 1107 10-11 0.11365154851893067 1107 12-13 0.12724975127539295 1107 14-15 0.17538459056958544 1107 16-17 0.15286469627079668 1107 18-19 0.14500600344904058 1107 20-21 0.17547198206889192 1107 22-23 0.1391731161920049 1107 24-25 0.1970316711655542 1107 26-27 0.08270713127321727 1107 28-29 0.10366445635448684 1107 30-31 0.1568882789656243 1107 32-33 0.12657135400736763 1107 34-35 0.13969770153942562 1107 36-37 0.13145785482394245 1107 38-39 0.24919445959523756 1107 40-41 0.18290051990708633 1107 42-43 0.23509872357094252 1107 44-45 0.1653496853770804 1107 46-47 0.1281969478037226 1107 48-49 0.1294171041792893 1107 50-51 0.14376569094937253 1107 52-53 0.10481208110817164 1107 54-55 0.17792051133145037 1107 56-57 0.19545539866444983 1107 58-59 0.20098665391741832 1107 60-61 0.22035501330798724 1107 62-63 0.22538575179436293 1107 64-65 0.221851479793564 1107 66-67 0.1968337747524771 1107 68-69 0.1814273756564475 1107 70-71 0.2113656087479754 1107 72-73 0.20832208396397078 1107 74-75 0.2535013142571003 1107 76-77 0.2716418040593638 1107 78-79 0.21640800523326575 1107 80-81 0.2410788330548499 1107 82-83 0.30630781097205073 1107 84-85 0.27090328210712045 1107 86-87 0.26082892374751765 1107 88-89 0.19832254572419572 1107 90-91 0.2906981673711364 1107 92-93 0.23748215838678277 1107 94-95 0.24661624043985597 1107 96-97 0.32380617316051286 1107 98-99 0.28639712592111266 1107 100-101 0.5888665659387833 1108 1 -0.07532333874976516 1108 2 -0.04717746415113311 1108 3 -0.034038463723540247 1108 4 -0.03211077692265718 1108 5 -0.009899009237166467 1108 6 0.013440260704449258 1108 7 0.006446322200091004 1108 8 0.04596766580020528 1108 9 0.029249351850253902 1108 10-11 0.06594881926884 1108 12-13 0.034746639281884484 1108 14-15 0.005975807900590269 1108 16-17 0.045786743326019064 1108 18-19 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-1.5394015323458916 2316 64-65 -1.5269970007258635 2316 66-67 -1.4474607883509236 2316 68-69 -1.3456270756469522 2316 70-71 -1.279032531535627 2316 72-73 -1.2158064709529057 2316 74-75 -1.179272879081985 2316 76-77 -1.1655674125408169 2316 78-79 -1.1553673811562852 2316 80-81 -1.217125538511926 2316 82-83 -1.1228577838806402 2316 84-85 -1.3065774536846106 2316 86-87 -1.3928616366480533 2316 88-89 -1.5294637337116583 2316 90-91 -1.4742579609708173 2316 92-93 -1.624304140905938 2316 94-95 -1.7178399573495717 2316 96-97 -1.6841269448046248 2316 98-99 -1.6508819037947084 2316 100-101 -1.9566843584805333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 380094.0 3 57816.0 4 49956.0 5 66073.0 6 134300.0 7 260832.0 8 448387.0 9 724828.0 10 858588.0 11 667699.0 12 453261.0 13 329694.0 14 274129.0 15 247176.0 16 243185.0 17 249855.0 18 265953.0 19 292172.0 20 328003.0 21 375755.0 22 438787.0 23 521987.0 24 659871.0 25 692980.0 26 554378.0 27 648301.0 28 768852.0 29 922534.0 30 1116042.0 31 1377449.0 32 1723527.0 33 2216265.0 34 2956698.0 35 4176929.0 36 6576733.0 37 1.2632363E7 38 2.1116576E7 39 8112605.0 40 18454.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.725373709077843 19.216230756734895 8.29771140142226 40.760684132765 2 15.967805236962365 16.923144568403234 55.20990167866615 11.899148515968246 3 19.174351879023096 23.316365727731547 36.68272175248069 20.82656064076467 4 20.030936237226374 29.01418260124534 34.82852828606139 16.126352875466896 5 18.964326644181558 32.02610456530835 34.26792475088419 14.7416440396259 6 16.13619515196344 35.36839278292898 34.3790595563663 14.116352508741285 7 13.874760765016756 17.857158107667704 51.92615145495497 16.34192967236057 8 13.051757105726683 23.661745398258475 45.13071748970713 18.15578000630771 9 16.307994134547062 21.733257501511925 42.63489941244888 19.323848951492135 10-11 20.168027214573 32.12328773622632 31.301941322474253 16.40674372672643 12-13 17.82635674380051 26.224399085351628 38.33534704575624 17.613897125091622 14-15 18.04701286007777 26.872687737953466 38.46535207792639 16.614947324042372 16-17 19.00247995076392 27.143336023983984 36.019493048445995 17.834690976806105 18-19 19.807976305642654 28.10033433634327 34.741356058837184 17.35033329917689 20-21 18.99268247229731 29.142131448385822 34.1586917251865 17.70649435413036 22-23 20.801504794962483 28.692960443849948 34.1686567296067 16.336878031580866 24-25 19.020055186389502 30.073666574944806 33.76249402621539 17.1437842124503 26-27 18.584222107260732 30.5474346803564 33.10703394956841 17.76130926281446 28-29 19.67417489396353 30.64157588806421 31.15875929498903 18.525489922983226 30-31 19.403190488423448 28.987412974923586 32.82724627521202 18.782150261440947 32-33 19.81582324167269 29.41953029140147 32.50344141039504 18.2612050565308 34-35 18.57133288632397 31.439374764793882 30.732546457523966 19.256745891358186 36-37 17.89885569743439 30.237409734986453 32.504243033982675 19.35949153359648 38-39 18.965892327551494 28.53111103276803 32.67277154087147 19.830225098809002 40-41 20.63449178405002 28.85880380882378 31.231513849174792 19.27519055795141 42-43 20.281117586205237 30.170264038321776 31.580841310820656 17.967777064652335 44-45 20.78589301014411 29.67724542335743 31.146287757779596 18.390573808718866 46-47 19.003576120227443 29.290331326216368 31.71869841377049 19.9873941397857 48-49 20.143672547420092 29.508379204619445 29.976108782791982 20.371839465168485 50-51 19.655648875368076 29.62661684791355 30.88652918398414 19.831205092734233 52-53 18.571406814156617 30.811606771212773 32.402076907157564 18.214909507473042 54-55 18.819812529968807 30.36954677019698 31.48676891134286 19.323871788491353 56-57 19.721930895921396 30.153087968743147 31.179844301296583 18.945136834038873 58-59 19.533014689420323 30.364909989142248 30.385236676528056 19.716838644909377 60-61 20.07488932630237 31.336429201564986 29.772466154104087 18.816215318028558 62-63 18.461687954552687 33.25808450258906 29.974454807825257 18.305772735032996 64-65 18.226474264967496 32.534550431508826 30.623822532062235 18.615152771461442 66-67 18.693793059249614 31.513459444512915 30.79969816407767 18.9930493321598 68-69 20.325350922109816 31.51504595534867 29.30090851588044 18.85869460666107 70-71 19.72745705623493 31.85014557362416 29.313257176870618 19.109140193270292 72-73 18.878028723869196 32.57222497910386 29.812587102852532 18.737159194174417 74-75 19.30065970058272 31.15606464031399 30.691179568115956 18.852096090987335 76-77 19.66975996670099 31.454139226522752 29.706283516574 19.169817290202253 78-79 19.535593232307754 32.10686728553765 28.952815140219073 19.404724341935513 80-81 19.731456775759877 31.37473877502558 30.55671417623574 18.337090272978802 82-83 19.407223924817785 32.045463835336996 30.048192079022517 18.4991201608227 84-85 19.445823995984295 31.88482183019037 29.68819241735426 18.981161756471078 86-87 20.250277569560705 31.92113845205465 28.877094659541054 18.951489318843592 88-89 20.034071807379327 31.459242570165202 29.709754884265223 18.796930738190248 90-91 20.294767136502383 31.596251597078695 29.365204763244545 18.743776503174377 92-93 19.64868055827533 32.464336729029824 29.014039753468378 18.872942959226467 94-95 19.18702911413485 32.30958818166864 29.12435673007504 19.37902597412147 96-97 19.73348077486068 31.407748079551762 29.629772202203185 19.228998943384365 98-99 20.259880598008944 31.737186459960615 29.036899297794672 18.966033644235768 100-101 20.979578660935136 32.463693230310675 27.73335188630594 18.823376222448246 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 90574.0 1 54888.5 2 17444.0 3 17142.0 4 20697.0 5 26225.0 6 32976.5 7 40403.0 8 49138.5 9 58259.5 10 67972.0 11 78748.5 12 89755.5 13 101156.5 14 113986.5 15 128351.0 16 144018.0 17 162391.5 18 185233.0 19 215955.0 20 257975.0 21 311169.0 22 377664.5 23 461070.0 24 571836.5 25 707300.0 26 858702.5 27 1046026.5 28 1319674.0 29 1619732.5 30 1933260.5 31 2311351.0 32 2856240.0 33 3367316.0 34 3693829.5 35 4120081.0 36 4393594.5 37 4236633.5 38 3809637.5 39 3272166.5 40 2961852.5 41 2796928.0 42 2582975.0 43 2449823.0 44 2315016.5 45 2159209.5 46 2016443.0 47 1852725.0 48 1697020.5 49 1555757.5 50 1382937.5 51 1212637.0 52 1056797.0 53 920515.5 54 795855.5 55 675198.5 56 568114.5 57 457495.5 58 358428.0 59 276270.0 60 201434.0 61 142815.5 62 100478.5 63 68016.5 64 45643.5 65 29891.0 66 19764.5 67 13906.0 68 9882.0 69 6699.5 70 4975.5 71 4708.0 72 3996.0 73 4253.5 74 4263.5 75 2698.0 76 1994.0 77 1683.0 78 1388.0 79 984.0 80 638.0 81 408.0 82 310.5 83 238.0 84 158.5 85 123.5 86 99.5 87 79.0 88 66.5 89 49.5 90 42.0 91 27.5 92 17.0 93 14.0 94 10.0 95 7.5 96 9.0 97 9.5 98 5.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36745111553784804 2 0.3094953011794695 3 0.2829071990028765 4 0.28404056436347397 5 0.2807932967849603 6 0.21776438759650898 7 0.23145268212467918 8 0.22867607223767855 9 0.22832984129219774 10-11 0.22963091226701243 12-13 0.2375712321143484 14-15 0.23267395768627763 16-17 0.24257332255130498 18-19 0.24402384086782136 20-21 0.23330826359811555 22-23 0.20848377530006557 24-25 0.2014800372095479 26-27 0.17951127797939945 28-29 0.17604288243375252 30-31 0.19686204672773414 32-33 0.2096610957611635 34-35 0.21017029869465387 36-37 0.20433711874208024 38-39 0.19860740233376156 40-41 0.18306623126141658 42-43 0.1781892438028076 44-45 0.16815463247470178 46-47 0.17872076240270587 48-49 0.2006678821987618 50-51 0.20504986760250368 52-53 0.23079470808180252 54-55 0.2407623724106845 56-57 0.24365394179130181 58-59 0.22201247900180318 60-61 0.21217059388358422 62-63 0.2045325498812286 64-65 0.19845795499205987 66-67 0.20285684620368655 68-69 0.20377381722335844 70-71 0.21629290607821544 72-73 0.2478398198235799 74-75 0.25638536759319197 76-77 0.23469318738003891 78-79 0.2157458341350631 80-81 0.20508232675364252 82-83 0.1917706936251458 84-85 0.17973713957274046 86-87 0.20094243251881105 88-89 0.1769713494027861 90-91 0.17120105905554392 92-93 0.21780969516164028 94-95 0.2178245722725789 96-97 0.21976941641164704 98-99 0.20891724021423203 100-101 0.18879391897278905 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7.3939087E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.099799904014205 #Duplication Level Percentage of deduplicated Percentage of total 1 78.86925382694335 28.47164281731562 2 9.145572223545075 6.60306654555375 3 3.498137336103292 3.7884617361027044 4 1.8744241552998815 2.706653477663063 5 1.2101533685997679 2.1843147229810187 6 0.82548957008599 1.788000498177297 7 0.6312043470850132 1.5950445439819037 8 0.47537492451338836 1.3728751723455344 9 0.38969018488752005 1.2660963929097997 >10 2.617378817699775 18.360162880923124 >50 0.2769152554878769 6.919762118949301 >100 0.16769578715439018 11.304757244089659 >500 0.01138332032570761 2.796041653928152 >1k 0.006256016599549511 4.345987181205159 >5k 6.538139199597772E-4 1.6345560109548267 >10k+ 4.1705174958362747E-4 4.862577002919448 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 888383 1.2015065860902503 Illumina Single End PCR Primer 1 (100% over 50bp) CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 178290 0.24113091902257328 No Hit GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG 92148 0.12462691079753256 No Hit AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA 84222 0.11390727613393441 No Hit AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA 83895 0.11346502019966788 No Hit ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 83343 0.1127184597234748 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 78767 0.10652958157300481 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.4300785185513583 1.3524646307845268E-6 0.0 2.02869694617679E-5 0.0 2 1.4387275298652253 1.3524646307845266E-5 0.0 3.651654503118222E-5 0.0 3 1.4388925305501812 1.3524646307845266E-5 0.0 4.733626207745844E-5 0.0 4 1.4404924962083991 1.4877110938629793E-5 0.0 5.680351449295012E-5 0.0 5 1.440726472589525 1.4877110938629793E-5 0.0 6.627076690844181E-5 0.0 6 1.440990203192528 1.7582040200198847E-5 0.0 8.655773637020971E-5 0.0 7 1.4412985651283468 1.8934504830983374E-5 0.0 9.60249887857014E-5 0.0 8 1.4432298846211071 3.1106686508044114E-5 0.0 1.0549224120119309E-4 0.0 9 1.4436789028785275 3.1106686508044114E-5 0.0 1.1090209972433119E-4 0.0 10-11 1.4447906288050323 3.651654503118222E-5 0.0 1.1834065519364609E-4 0.0 12-13 1.4464413118869048 4.395510050049712E-5 0.0 1.4268501854776758E-4 0.0 14-15 1.4488554612528555 4.4631332815889384E-5 0.0 1.535047355940438E-4 0.0 16-17 1.4522136309310933 4.4631332815889384E-5 0.0 2.0692708851003262E-4 0.0 18-19 1.4572698199532812 4.4631332815889384E-5 0.0 2.2721405797180048E-4 0.0 20-21 1.4662069062335055 4.598379744667391E-5 0.0 2.5696827984906005E-4 0.0 22-23 1.4767020317683932 4.733626207745844E-5 0.0 3.266202083344632E-4 0.0 24-25 1.4828773852725554 4.733626207745844E-5 0.0 3.387923900115239E-4 0.0 26-27 1.4889107299904853 4.733626207745844E-5 0.0 3.5164080400397696E-4 0.0 28-29 1.4977254182216235 4.8688726708242965E-5 0.0 3.6854661188878354E-4 1.3524646307845268E-6 30-31 1.5082482692814425 5.342235291598881E-5 0.0 6.228099624762746E-4 1.3524646307845268E-6 32-33 1.5201364333860385 5.477481754677333E-5 0.0 7.228923451543295E-4 1.3524646307845268E-6 34-35 1.5314558861133896 5.545104986216559E-5 0.0 9.588974232262295E-4 1.3524646307845268E-6 36-37 1.5439120853629151 5.6127282177557856E-5 0.0 0.0010150247054037873 1.3524646307845268E-6 38-39 1.5583868380738863 5.680351449295012E-5 0.0 0.0010413977657040855 1.3524646307845268E-6 40-41 1.5763847611480515 5.680351449295012E-5 0.0 0.0010772380784198755 1.3524646307845268E-6 42-43 1.5972309747346487 5.815597912373465E-5 0.0 0.0010975250478816433 2.02869694617679E-6 44-45 1.616696321933215 5.883221143912691E-5 0.0 0.0011130783911356655 2.7049292615690536E-6 46-47 1.6375986628019898 5.9508443754519174E-5 0.0 0.0011888164104595991 2.7049292615690536E-6 48-49 1.66126814630535 5.9508443754519174E-5 0.0 0.0012584683389450023 3.3811615769613166E-6 50-51 1.6879793227633444 6.018467606991144E-5 0.0 0.0013484072368921732 4.05739389235358E-6 52-53 1.7159658733681686 6.221337301608823E-5 0.0 0.0014660716597704269 4.05739389235358E-6 54-55 1.744368306846959 6.221337301608823E-5 0.0 0.0015242276388941615 4.05739389235358E-6 56-57 1.7778789992362225 6.424206996226501E-5 0.0 0.001556010557717598 4.05739389235358E-6 58-59 1.8143948680350894 6.897569617001086E-5 0.0 0.001574268830233189 4.733626207745844E-6 60-61 1.8521090204968313 7.032816080079539E-5 0.0 0.00160740421368741 5.409858523138107E-6 62-63 1.8939204645575352 7.168062543157992E-5 0.0 0.001661502798918791 5.409858523138107E-6 64-65 1.9380574715508727 7.235685774697217E-5 0.0 0.0016885520915344817 5.409858523138107E-6 66-67 1.9913750084579758 7.438555469314897E-5 0.0 0.0017068103640500727 5.409858523138107E-6 68-69 2.047055977307375 7.57380193239335E-5 0.0 0.0017602327169660616 5.409858523138107E-6 70-71 2.1132496537318617 7.709048395471802E-5 0.0 0.001805540282097343 5.409858523138107E-6 72-73 2.1870015787454884 7.979541321628708E-5 0.0 0.0018454379887054865 5.409858523138107E-6 74-75 2.2680304397050506 7.979541321628708E-5 0.0 0.0019062988970907904 5.409858523138107E-6 76-77 2.3603388556853564 8.317657479324839E-5 0.0 0.0019556638561144257 5.409858523138107E-6 78-79 2.4659048062089273 8.520527173942519E-5 0.0 0.001998266491984138 5.409858523138107E-6 80-81 2.5982373842403543 8.588150405481744E-5 0.0 0.002059803632684834 5.409858523138107E-6 82-83 2.740139325766898 8.926266563177877E-5 0.0 0.0021855828433477954 5.409858523138107E-6 84-85 2.909706607548454 8.926266563177877E-5 0.0 0.0024114444366888114 5.409858523138107E-6 86-87 3.1173369776664943 9.196759489334782E-5 0.0 0.002488534920643529 5.409858523138107E-6 88-89 3.3651010594707502 9.39962918395246E-5 0.0 0.0025967320911062914 5.409858523138107E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGATCT 9120 0.0 56.811913 2 CGCGTGT 48625 0.0 54.740143 1 TCCGATC 9560 0.0 54.02343 1 GCGTGTT 61370 0.0 41.63227 2 AGAGCGT 253295 0.0 40.27795 9 CCGTATC 137100 0.0 40.208965 48-49 CGCCGTA 137220 0.0 40.07601 46-47 GTCGCCG 137060 0.0 39.69201 44-45 CTGGAAT 195910 0.0 38.70599 1 TGGTCGC 138735 0.0 38.11404 42-43 CGTATCA 135875 0.0 37.882603 50-51 AAGAGCG 271970 0.0 37.795513 8 TCGCCGT 135040 0.0 37.40737 46-47 TCGGTGG 149625 0.0 36.77859 38-39 GGTCGCC 139450 0.0 36.704308 44-45 GTTTTTT 973595 0.0 36.221504 1 GTGGTCG 139405 0.0 36.118706 42-43 GCCGTAT 142810 0.0 36.069782 48-49 TCTCGGT 153505 0.0 35.33851 36-37 GGTGGTC 152130 0.0 34.82823 40-41 >>END_MODULE