FastQCFastQC Report
Fri 27 May 2016
SRR1233063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233063_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73939087
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA8926731.2073086593563158TruSeq Adapter, Index 19 (97% over 40bp)
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1849190.25009640706004393No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT1669530.22579802750336908TruSeq Adapter, Index 19 (97% over 40bp)
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG982940.13293915841833426No Hit
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA900440.12178132521436193No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA896230.12121193760480163No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT810970.10968082416273277No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT747490.1010953786865126No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA741710.10031365412991915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT472250.058.171331
GCGTGTT597350.044.0073432
TGCCGTC1547350.039.9296350-51
TATGCCG1564600.039.71859448-49
CCGTCTT1548950.039.70637552-53
CTGGAAT1975450.039.285421
CTCGTAT1515150.038.0487644-45
CGTATGC1659850.037.53014846-47
CGATCTC1499750.037.13382740-41
AACGATC1524200.036.57103738-39
ATCTCGT1551000.036.5414942-43
GAAACGA1731950.035.37880736-37
GCCGTCT1544850.033.94605352-53
TGCTTGA1805300.033.90638460-61
ATGCCGT1557450.033.87080450-51
CGTCTTC1577700.033.29421254-55
AGTCACG1894200.032.88121428-29
TCACGTG1906450.032.69115430-31
TCTCGTA1483450.032.6327344-45
GTTTTTT8356050.032.614591
Produced by FastQC (version 0.11.5)