##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233063_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 73939087 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37941651889751 34.0 31.0 34.0 31.0 34.0 2 32.658351664526236 34.0 31.0 34.0 31.0 34.0 3 32.22873654093132 34.0 31.0 34.0 30.0 34.0 4 35.87147813983692 37.0 35.0 37.0 35.0 37.0 5 35.949404122341946 37.0 35.0 37.0 35.0 37.0 6 36.01179539855557 37.0 35.0 37.0 35.0 37.0 7 36.09305343464682 37.0 35.0 37.0 35.0 37.0 8 36.08501297831822 37.0 35.0 37.0 35.0 37.0 9 37.80355117179091 39.0 38.0 39.0 35.0 39.0 10-11 37.752078795617265 39.0 38.0 39.0 35.0 39.0 12-13 37.71877759323698 39.0 38.0 39.0 35.0 39.0 14-15 39.139735164703886 40.5 39.0 41.0 36.0 41.0 16-17 39.03264866524522 40.0 38.5 41.0 36.0 41.0 18-19 38.87844695052834 40.0 38.0 41.0 35.0 41.0 20-21 38.758999580289654 40.0 38.0 41.0 34.5 41.0 22-23 38.565655442837695 40.0 38.0 41.0 34.0 41.0 24-25 38.28879917735527 40.0 38.0 41.0 34.0 41.0 26-27 38.12201792537687 40.0 38.0 41.0 34.0 41.0 28-29 37.906397058973695 40.0 38.0 41.0 33.0 41.0 30-31 37.72167626305691 40.0 38.0 41.0 33.0 41.0 32-33 37.60821814042686 40.0 38.0 41.0 33.0 41.0 34-35 37.49555594323203 40.0 38.0 41.0 32.5 41.0 36-37 37.39612912044748 40.0 38.0 41.0 32.0 41.0 38-39 37.327698785352865 40.0 38.0 41.0 32.0 41.0 40-41 37.25399218819134 40.0 38.0 41.0 32.0 41.0 42-43 37.105049944692986 40.0 38.0 41.0 31.0 41.0 44-45 36.87270134942294 40.0 37.5 41.0 30.5 41.0 46-47 36.98165543347864 40.0 38.0 41.0 31.0 41.0 48-49 36.992265640228965 40.0 38.0 41.0 31.0 41.0 50-51 36.96463526794698 40.0 38.0 41.0 31.0 41.0 52-53 36.87356125995984 40.0 37.0 41.0 31.0 41.0 54-55 36.701430990079714 40.0 37.0 41.0 30.5 41.0 56-57 36.516476758767666 40.0 37.0 41.0 30.0 41.0 58-59 36.27931181379072 40.0 36.0 41.0 29.0 41.0 60-61 36.17509825351238 39.5 36.0 41.0 29.0 41.0 62-63 35.957278570399446 39.0 36.0 41.0 29.0 41.0 64-65 35.73240944941611 39.0 35.0 41.0 28.0 41.0 66-67 35.36551240482588 38.5 35.0 40.5 28.0 41.0 68-69 35.06291936631568 38.0 35.0 40.0 28.0 41.0 70-71 34.71933498448527 37.5 34.5 40.0 27.5 41.0 72-73 34.21053522070133 37.0 34.0 39.5 26.0 41.0 74-75 33.64603149616927 36.5 34.0 39.0 25.5 41.0 76-77 32.82536433672761 35.5 32.5 39.0 23.5 40.0 78-79 32.87187756997865 35.5 34.0 38.0 24.0 39.5 80-81 32.61017794282475 35.0 34.0 37.0 24.0 39.0 82-83 32.27546293613282 35.0 33.0 37.0 22.5 39.0 84-85 31.918096121473614 35.0 33.0 36.5 21.5 38.5 86-87 31.577212253107753 35.0 33.0 36.0 20.5 37.0 88-89 31.226214052386123 35.0 32.5 36.0 19.0 37.0 90-91 30.927695442060298 35.0 32.0 35.0 18.0 36.5 92-93 30.659882924981208 35.0 32.0 35.0 12.5 36.0 94-95 30.38780710803205 35.0 32.0 35.0 4.5 36.0 96-97 30.08802616808076 35.0 32.0 35.0 2.0 36.0 98-99 29.761659235797705 34.0 31.5 35.0 2.0 35.5 100-101 28.10281631554363 33.0 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3571985861098952 1101 2 0.3076500931400261 1101 3 0.7232655429021548 1101 4 0.3947487749320331 1101 5 0.29653670541181754 1101 6 0.24534470938747432 1101 7 0.19935505314707314 1101 8 0.1840509208042178 1101 9 0.3387843062131566 1101 10-11 0.3123727140170942 1101 12-13 0.302906293604309 1101 14-15 0.3884149685174023 1101 16-17 0.4069825369074991 1101 18-19 0.39854656140292377 1101 20-21 0.40633079037137776 1101 22-23 0.4288615160178182 1101 24-25 0.5256576820232368 1101 26-27 0.4735284510009805 1101 28-29 0.4351763564083484 1101 30-31 0.450680522106218 1101 32-33 0.43942007727751076 1101 34-35 0.4062603599517942 1101 36-37 0.3936561867699879 1101 38-39 0.4134618130821437 1101 40-41 0.3791443821889757 1101 42-43 0.3746194344645133 1101 44-45 0.4071695145316525 1101 46-47 0.451877081790947 1101 48-49 0.45620925716199423 1101 50-51 0.4456420586086054 1101 52-53 0.40011172235247017 1101 54-55 0.43838516397627814 1101 56-57 0.4484415606719949 1101 58-59 0.58728430039681 1101 60-61 0.4963571724713134 1101 62-63 0.36510378788629083 1101 64-65 0.4267593597574688 1101 66-67 0.36905216543214436 1101 68-69 0.4013396496418835 1101 70-71 0.4316846043352811 1101 72-73 0.3689785242827952 1101 74-75 0.40677233011830793 1101 76-77 0.4625001194947558 1101 78-79 0.504654450085603 1101 80-81 0.47588400977809187 1101 82-83 0.44144457740380005 1101 84-85 0.3726498030935197 1101 86-87 0.3752684204615946 1101 88-89 0.4281204950525037 1101 90-91 0.485296003602361 1101 92-93 0.44061612966576646 1101 94-95 0.42706692860472373 1101 96-97 0.5444887730112526 1101 98-99 0.5571562210620193 1101 100-101 0.9641191132564835 1102 1 0.324010585675893 1102 2 0.2728956274632779 1102 3 0.6986941716828952 1102 4 0.38652026189013355 1102 5 0.2852886709687965 1102 6 0.22230530897967782 1102 7 0.19790614280028507 1102 8 0.20197775539352136 1102 9 0.3006535796865677 1102 10-11 0.26760687538801164 1102 12-13 0.27989392800897406 1102 14-15 0.370955341670431 1102 16-17 0.3355217862365123 1102 18-19 0.32522219559859167 1102 20-21 0.34975307164815206 1102 22-23 0.3697017940118599 1102 24-25 0.4256850752867578 1102 26-27 0.4019878108605255 1102 28-29 0.3652834667722047 1102 30-31 0.36235328908417586 1102 32-33 0.4050679684765228 1102 34-35 0.3791669824840014 1102 36-37 0.3743027858754857 1102 38-39 0.3401206867598461 1102 40-41 0.41272399094046364 1102 42-43 0.39572111024844503 1102 44-45 0.38651082812952353 1102 46-47 0.42664131282563034 1102 48-49 0.37282667606326214 1102 50-51 0.43393690243325267 1102 52-53 0.4143801755676506 1102 54-55 0.45780692493414676 1102 56-57 0.42303757406951803 1102 58-59 0.5421025000933 1102 60-61 0.43674297892620473 1102 62-63 0.37573815020437706 1102 64-65 0.42397680150723716 1102 66-67 0.3914521175666792 1102 68-69 0.3631456804825888 1102 70-71 0.3494534472674147 1102 72-73 0.30629224918494913 1102 74-75 0.37076053215456994 1102 76-77 0.3805286177680429 1102 78-79 0.4027414408222967 1102 80-81 0.44356959046138655 1102 82-83 0.39168972773323674 1102 84-85 0.3141403738376276 1102 86-87 0.3490048728321469 1102 88-89 0.335263086110281 1102 90-91 0.4081344344614912 1102 92-93 0.42491489782258185 1102 94-95 0.4187285565294374 1102 96-97 0.4742390803359662 1102 98-99 0.504682938934824 1102 100-101 0.8909907917624444 1103 1 0.2663647578615098 1103 2 0.22616097857451933 1103 3 0.656331080344593 1103 4 0.37913208210932936 1103 5 0.25905963860841297 1103 6 0.202308190495323 1103 7 0.15584443292517847 1103 8 0.157354858701936 1103 9 0.2634164441915985 1103 10-11 0.24112728018847918 1103 12-13 0.25417739725912725 1103 14-15 0.3241604764354875 1103 16-17 0.3386857602358262 1103 18-19 0.35306814225011607 1103 20-21 0.3228596379951867 1103 22-23 0.3488421999566498 1103 24-25 0.3983595257763568 1103 26-27 0.37139425566110873 1103 28-29 0.3678791073100314 1103 30-31 0.3689919296054356 1103 32-33 0.3766768046440916 1103 34-35 0.407198801168029 1103 36-37 0.3579085198998797 1103 38-39 0.3718634396719054 1103 40-41 0.40880117732689314 1103 42-43 0.3693107981818642 1103 44-45 0.3713641746512977 1103 46-47 0.3938173437989647 1103 48-49 0.3878950477220471 1103 50-51 0.38096104717148904 1103 52-53 0.3699074717241544 1103 54-55 0.44625523082555674 1103 56-57 0.45125610852321785 1103 58-59 0.5956822812243843 1103 60-61 0.448927011289598 1103 62-63 0.4153738739183481 1103 64-65 0.43191218465063486 1103 66-67 0.3019725736817165 1103 68-69 0.316462497170356 1103 70-71 0.36851884519779077 1103 72-73 0.2643002655500055 1103 74-75 0.29328234999604774 1103 76-77 0.34373913250610855 1103 78-79 0.4056657782024331 1103 80-81 0.44089014594732134 1103 82-83 0.4183382095302619 1103 84-85 0.37964115622341765 1103 86-87 0.3924975662037937 1103 88-89 0.39784842936024134 1103 90-91 0.4460803155653217 1103 92-93 0.4827691423861431 1103 94-95 0.5001649700173729 1103 96-97 0.4914835700988327 1103 98-99 0.4976603993630171 1103 100-101 0.866254404268556 1104 1 0.35039712952873714 1104 2 0.3079763479465356 1104 3 0.7136378842271398 1104 4 0.4203308948826603 1104 5 0.32798471058747225 1104 6 0.2603628164457277 1104 7 0.17917658583904483 1104 8 0.22481816864618054 1104 9 0.3154317228441741 1104 10-11 0.3072670601098153 1104 12-13 0.3117497654080452 1104 14-15 0.34950181267019076 1104 16-17 0.4093250612403878 1104 18-19 0.3430744449365548 1104 20-21 0.36071387902404695 1104 22-23 0.42678923538023383 1104 24-25 0.48049600969804374 1104 26-27 0.39115795347074567 1104 28-29 0.42512605705265116 1104 30-31 0.42551346995868755 1104 32-33 0.47122304217130306 1104 34-35 0.45309997005767144 1104 36-37 0.4358059936329042 1104 38-39 0.4724519982987516 1104 40-41 0.4809244363880296 1104 42-43 0.4499240196722738 1104 44-45 0.4591790984231636 1104 46-47 0.3987843624212246 1104 48-49 0.3046915971696933 1104 50-51 0.4250183259304521 1104 52-53 0.4153014430296693 1104 54-55 0.4805004049065076 1104 56-57 0.536612736306175 1104 58-59 0.6531717852849894 1104 60-61 0.48864037366811885 1104 62-63 0.4836086022740673 1104 64-65 0.4920200128808432 1104 66-67 0.432266967312934 1104 68-69 0.4320568329865253 1104 70-71 0.5006720004783105 1104 72-73 0.42982555194661387 1104 74-75 0.42000370778079343 1104 76-77 0.4123697052526083 1104 78-79 0.44843560404810034 1104 80-81 0.4440205244924371 1104 82-83 0.4713965556523618 1104 84-85 0.4889254827684191 1104 86-87 0.5092761000170754 1104 88-89 0.5123752456109969 1104 90-91 0.554236156679913 1104 92-93 0.5406052687710208 1104 94-95 0.571625917824413 1104 96-97 0.5862448841825589 1104 98-99 0.62903210895713 1104 100-101 0.9906145399899593 1105 1 0.2716812964317228 1105 2 0.23088725944191424 1105 3 0.6638634790106366 1105 4 0.3791578084389329 1105 5 0.24544417994172818 1105 6 0.2084455337232498 1105 7 0.1686999680945931 1105 8 0.1795009801942271 1105 9 0.2943771990557309 1105 10-11 0.2245719583831729 1105 12-13 0.2562493831887167 1105 14-15 0.3356246236437812 1105 16-17 0.3341549706045015 1105 18-19 0.3271723133752218 1105 20-21 0.3205584859161732 1105 22-23 0.3307996120686454 1105 24-25 0.3634407321883799 1105 26-27 0.3595070667926521 1105 28-29 0.32897835984638135 1105 30-31 0.36195810681675056 1105 32-33 0.35839950632067286 1105 34-35 0.36156669361246685 1105 36-37 0.3468751392128411 1105 38-39 0.35472188772558866 1105 40-41 0.35549973245171174 1105 42-43 0.3771624509375684 1105 44-45 0.35330876262246136 1105 46-47 0.40841050698036696 1105 48-49 0.3749861082515551 1105 50-51 0.39675053611985334 1105 52-53 0.34622391163134836 1105 54-55 0.3985475203063089 1105 56-57 0.4461353883474075 1105 58-59 0.5773761046098471 1105 60-61 0.4352372855760436 1105 62-63 0.39354793732605486 1105 64-65 0.3943901781672139 1105 66-67 0.4254750831806575 1105 68-69 0.40682594307747877 1105 70-71 0.36691384424516826 1105 72-73 0.3339400863481785 1105 74-75 0.31902140018780045 1105 76-77 0.34831852837505295 1105 78-79 0.32713318285890836 1105 80-81 0.4086612229660105 1105 82-83 0.40855788393078285 1105 84-85 0.38294963921547165 1105 86-87 0.42323467614487953 1105 88-89 0.4147006731239742 1105 90-91 0.41020477954215195 1105 92-93 0.38993475876204897 1105 94-95 0.42623168318797156 1105 96-97 0.4744961498576039 1105 98-99 0.5790062220736125 1105 100-101 0.9431371453746387 1106 1 0.177009683403476 1106 2 0.14974383324165075 1106 3 0.5701389100236867 1106 4 0.3180112349019666 1106 5 0.18737229883888062 1106 6 0.1632032530969667 1106 7 0.105639437203493 1106 8 0.11323693414486513 1106 9 0.22056950513399443 1106 10-11 0.14841056732765168 1106 12-13 0.1374632352008689 1106 14-15 0.17024966151470977 1106 16-17 0.1237900682917612 1106 18-19 0.20566151363720309 1106 20-21 0.19810231885177387 1106 22-23 0.1856836518172429 1106 24-25 0.2340925176263653 1106 26-27 0.14851100360277059 1106 28-29 0.19008190586710327 1106 30-31 0.1836296277655194 1106 32-33 0.16898926340110165 1106 34-35 0.1990650369548277 1106 36-37 0.19405746343267083 1106 38-39 0.200405031549451 1106 40-41 0.16724414949101885 1106 42-43 0.20367629112775631 1106 44-45 0.174659011386872 1106 46-47 0.20476834097131302 1106 48-49 0.1719638854489105 1106 50-51 0.20218132625225138 1106 52-53 0.24187210458197228 1106 54-55 0.26760862430457877 1106 56-57 0.2939750932237146 1106 58-59 0.356775833940155 1106 60-61 0.23094504890806178 1106 62-63 0.20592811217105833 1106 64-65 0.26317050560105315 1106 66-67 0.2608635543997124 1106 68-69 0.21450084651942092 1106 70-71 0.22561551044827866 1106 72-73 0.2144173220410508 1106 74-75 0.22012840972994496 1106 76-77 0.21579803995659574 1106 78-79 0.24030945912828372 1106 80-81 0.31118756716357865 1106 82-83 0.24596850141412574 1106 84-85 0.2765656757686621 1106 86-87 0.27876354411069215 1106 88-89 0.26850563221075063 1106 90-91 0.32513732520432015 1106 92-93 0.3241576017662524 1106 94-95 0.32451727331007874 1106 96-97 0.32738670427349703 1106 98-99 0.3205473683958395 1106 100-101 0.6592360568758373 1107 1 0.13035515326737368 1107 2 0.09847508532182303 1107 3 0.5353250562019412 1107 4 0.2962525835561465 1107 5 0.1676122760331893 1107 6 0.11562813076452727 1107 7 0.1051794620821127 1107 8 0.04274559371588538 1107 9 0.14398364989403234 1107 10-11 0.11037946430730727 1107 12-13 0.1277753509215671 1107 14-15 0.16894789143136535 1107 16-17 0.10707677990274789 1107 18-19 0.14614667274891247 1107 20-21 0.14690733810477496 1107 22-23 0.17545344961449416 1107 24-25 0.225656587035715 1107 26-27 0.2049845293369046 1107 28-29 0.20099243831282365 1107 30-31 0.13031258865286333 1107 32-33 0.17926109946610325 1107 34-35 0.20045227342512106 1107 36-37 0.1518658386535634 1107 38-39 0.17440206193535346 1107 40-41 0.20135137473322118 1107 42-43 0.17088749198430264 1107 44-45 0.20850246697475683 1107 46-47 0.19816054466314625 1107 48-49 0.21570068532864894 1107 50-51 0.1808957370056632 1107 52-53 0.16027570599969465 1107 54-55 0.19167380319231597 1107 56-57 0.23825212978987764 1107 58-59 0.3806636671887489 1107 60-61 0.26605841093481075 1107 62-63 0.23819767198273922 1107 64-65 0.22806551443593293 1107 66-67 0.2247277032561925 1107 68-69 0.15382533610485183 1107 70-71 0.21740853075262834 1107 72-73 0.16982709231362492 1107 74-75 0.17587330988389027 1107 76-77 0.18091348981093347 1107 78-79 0.2434749279685775 1107 80-81 0.27465417971954764 1107 82-83 0.2636600796189015 1107 84-85 0.19012362526099125 1107 86-87 0.2316877852546959 1107 88-89 0.23899708930500196 1107 90-91 0.27560050376520806 1107 92-93 0.28911830508437575 1107 94-95 0.26037711822937126 1107 96-97 0.28180585230444777 1107 98-99 0.2873839708192918 1107 100-101 0.6118348518882577 1108 1 0.11491832921204548 1108 2 0.07467227504766072 1108 3 0.49610226087476406 1108 4 0.2677597517601882 1108 5 0.14482183280012606 1108 6 0.08543892464584246 1108 7 0.047474193320596214 1108 8 0.01659472874829504 1108 9 0.1344470976876906 1108 10-11 0.048501583720671704 1108 12-13 0.05703743687005414 1108 14-15 0.0726804399632286 1108 16-17 0.05338075445862245 1108 18-19 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2316 56-57 -1.4145869669257465 2316 58-59 -1.092497556086954 2316 60-61 -1.2797260827202521 2316 62-63 -1.2817388482947862 2316 64-65 -1.3306161150328961 2316 66-67 -1.286295413785993 2316 68-69 -1.2638286597607546 2316 70-71 -1.2443485185357517 2316 72-73 -1.3419534804583435 2316 74-75 -1.3946820159149595 2316 76-77 -1.2739695750854167 2316 78-79 -1.1261845148126781 2316 80-81 -1.2099111291637925 2316 82-83 -1.2281048717829108 2316 84-85 -1.1698633265808986 2316 86-87 -1.1553705202842792 2316 88-89 -1.370783481700272 2316 90-91 -1.459191786384043 2316 92-93 -1.358492090876613 2316 94-95 -1.3928854950311162 2316 96-97 -1.5214625920891827 2316 98-99 -1.6107348944747493 2316 100-101 -2.066633674707777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9805.0 3 1296.0 4 3154.0 5 6614.0 6 17393.0 7 55802.0 8 186917.0 9 425078.0 10 682338.0 11 577622.0 12 374540.0 13 248492.0 14 191904.0 15 166135.0 16 154862.0 17 154664.0 18 163045.0 19 179475.0 20 201517.0 21 230007.0 22 267096.0 23 314717.0 24 374240.0 25 458710.0 26 581121.0 27 770929.0 28 943078.0 29 911641.0 30 1073538.0 31 1341707.0 32 1689414.0 33 2164515.0 34 2874533.0 35 4067531.0 36 6529743.0 37 1.3294357E7 38 2.3533587E7 39 8701599.0 40 16371.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.938873305395088 20.092321114905413 8.398393933388704 40.570411646310795 2 16.386075028712295 17.679978906108037 53.91217645454497 12.021769610634697 3 19.583148205819523 24.275773591341032 35.687046617939444 20.4540315849 4 20.27301153788178 30.15456945650015 33.49503102570774 16.077387979910334 5 19.232286048942115 32.96809776353648 33.080676373500495 14.718939814020915 6 16.17943702175478 36.48780016883094 33.28048846065874 14.052274348755548 7 13.888647511187772 18.25890901802344 51.306857945101626 16.54558552568716 8 13.179163350304544 24.128562821084152 44.261035624660614 18.43123820395069 9 16.694017805021005 21.890082654721294 41.88409637123862 19.531803169019078 10-11 20.40909025560459 32.84052790643736 30.18676373431552 16.56361810364253 12-13 17.847409314102023 26.909294268131823 37.411094892204986 17.83220152556117 14-15 17.422115991234786 28.26006358450166 36.737766318375016 17.58005410588854 16-17 18.43611036793179 28.365432458821576 35.06959634313034 18.128860830116295 18-19 19.86232556727939 28.4475379583976 34.03529528943854 17.65484118488447 20-21 19.036999270099685 28.62357677012728 33.73340034004309 18.606023619729946 22-23 19.83488525678257 30.052019067542695 33.72799318594556 16.385102489729178 24-25 18.2716638356515 30.092230253862763 33.6416853863145 17.994420524171236 26-27 18.606178159069618 29.03117849177609 33.53364517216938 18.82899817698491 28-29 19.021705436421442 30.807751462420143 30.80913976749508 19.361403333663336 30-31 19.30195324159196 29.081631749118024 32.633009790883676 18.983405218406336 32-33 19.14253243125301 30.327296205097014 31.299005080105335 19.23116628354464 34-35 18.65263629154203 30.786552913365117 30.432585688394692 20.128225106698157 36-37 18.872683822508098 29.844805176696138 31.908973376907063 19.373537623888705 38-39 19.028668828437116 30.465604072173626 31.45756655069328 19.048160548695982 40-41 19.881806090586466 29.792084230725614 30.87995524743642 19.446154431251507 42-43 20.123316437307427 31.242003700190995 30.579944090583133 18.054735771918445 44-45 19.124431711809017 29.924010943809026 31.649698704428538 19.301858639953423 46-47 19.046457865081575 29.376241009610144 31.466293156782733 20.111007968525556 48-49 20.17303838693703 29.750448629435972 30.666447251815466 19.41006573181153 50-51 18.9443198516357 30.484194143554475 31.501035967704276 19.070450037105545 52-53 19.28667985851651 30.063738817873148 31.359166634015917 19.290414689594424 54-55 19.75127133254228 30.314985703941606 30.664093791815965 19.26964917170015 56-57 19.833996309757783 29.436494744341058 30.8170767983209 19.912432147580258 58-59 19.64252874358384 29.40939869962882 30.94717559144143 20.000896965345916 60-61 20.31588353085378 29.626084993689783 30.43854593820451 19.619485537251926 62-63 19.20290824171002 31.625117997572794 29.919206318076785 19.252767442640394 64-65 18.356815765576954 30.971414143441784 32.00996194284181 18.66180814813945 66-67 19.338313794192672 31.038037579193418 30.59296959386971 19.030679032744196 68-69 20.130286032690982 32.093492100496235 28.980724987785607 18.795496879027176 70-71 19.399221912502014 32.69862803414226 28.88612439932538 19.01602565403035 72-73 18.462205661887378 33.484487436612284 29.37057358309706 18.68273331840328 74-75 18.922148467089738 31.970866425500372 30.279105035727177 18.827880071682713 76-77 19.254909842736883 32.18970917936373 29.370719508150756 19.18466146974863 78-79 19.199114765912803 32.72439700453928 28.6084820948744 19.468006134673523 80-81 19.33785678028869 31.94256997619935 30.28355214080109 18.43602110271087 82-83 19.034972303568704 32.51853417055187 29.810323405814916 18.63617012006452 84-85 19.115767092521867 32.33080526044015 29.444395154604347 19.109032492433634 86-87 19.888645736710973 32.32663589033261 28.650429629458117 19.134288743498296 88-89 19.606730124566454 31.908234955285298 29.510823087083992 18.974211833064256 90-91 19.85374892609228 32.01504019234429 29.140414255351644 18.990796626211786 92-93 19.152342591041375 32.90057576265739 28.796871239050613 19.15021040725062 94-95 18.63994938326975 32.770629558535475 28.87002489488324 19.719396163311533 96-97 19.13249066482478 31.845664306286125 29.40783987140606 19.61400515748304 98-99 19.611501471012076 32.22672872151583 28.81853243942396 19.34323736804813 100-101 20.100317669032112 33.05250929207346 27.584363248892203 19.262809790002226 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5168.0 1 6981.0 2 8191.0 3 9322.0 4 13315.0 5 18644.5 6 24676.0 7 31527.0 8 39422.0 9 47780.0 10 56793.5 11 66220.5 12 75800.5 13 85726.5 14 97040.0 15 109887.5 16 123776.0 17 140460.5 18 161769.0 19 190111.0 20 230886.0 21 283658.0 22 349396.0 23 433184.0 24 545140.0 25 681705.5 26 834151.5 27 1022978.5 28 1301396.0 29 1610661.0 30 1934016.0 31 2318130.0 32 2874145.0 33 3403627.5 34 3743100.5 35 4184396.5 36 4462840.0 37 4292578.0 38 3841714.0 39 3281152.0 40 2974613.0 41 2826291.5 42 2626059.5 43 2506672.0 44 2379234.5 45 2215032.0 46 2053926.0 47 1873305.0 48 1703885.0 49 1553457.0 50 1376797.0 51 1204652.5 52 1049895.0 53 915912.5 54 791678.5 55 672580.0 56 567432.0 57 456568.0 58 356083.0 59 272969.0 60 196346.5 61 136081.0 62 93136.5 63 61917.5 64 40923.0 65 26064.0 66 17071.0 67 12114.0 68 8452.0 69 5408.0 70 3820.0 71 3856.0 72 3310.5 73 3901.5 74 4080.0 75 2156.0 76 1256.0 77 1118.5 78 985.0 79 526.5 80 270.0 81 156.0 82 96.0 83 50.0 84 27.5 85 14.0 86 8.0 87 4.0 88 2.5 89 3.0 90 2.5 91 2.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011704228914809295 2 0.004046574175307304 3 0.010904922318015639 4 0.20084911245928694 5 0.0019177948464524591 6 4.192640355432033E-5 7 1.3795139234002173E-4 8 1.8528765441748016E-4 9 7.57380193239335E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.3524646307845268E-6 18-19 1.825827251559111E-5 20-21 3.015996126649494E-4 22-23 1.9137374525601055E-4 24-25 8.452903942403292E-5 26-27 5.2746120600596546E-5 28-29 9.467252415491687E-6 30-31 0.0 32-33 9.332005952413235E-5 34-35 8.155361723630695E-4 36-37 4.6660029762066174E-4 38-39 0.0 40-41 1.1157833203972346E-4 42-43 2.7184539078768986E-4 44-45 6.485067904611805E-4 46-47 0.007892983585258499 48-49 2.7049292615690536E-6 50-51 8.11478778470716E-6 52-53 0.0 54-55 7.100439311618765E-4 56-57 4.179115709124187E-4 58-59 0.001204369753713621 60-61 0.005693876095602857 62-63 0.007969397836897823 64-65 0.015335596448465749 66-67 0.010697995229505607 68-69 0.0037402409364346084 70-71 0.0018758684428981385 72-73 0.0013098619949148142 74-75 2.738740877338667E-4 76-77 1.0819717046276214E-5 78-79 9.169710196719091E-4 80-81 4.5983797446673907E-4 82-83 6.559453459304954E-5 84-85 3.651654503118222E-5 86-87 3.1106686508044114E-5 88-89 5.342235291598881E-5 90-91 8.11478778470716E-6 92-93 0.0010927914216738977 94-95 0.024443769504484143 96-97 0.0046119043909752366 98-99 2.6643553226455175E-4 100-101 0.06858010026550639 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7.3939087E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.75572538732972 #Duplication Level Percentage of deduplicated Percentage of total 1 76.70242909910789 25.891461332106143 2 9.916030631083252 6.694456138303921 3 3.8954158216040127 3.944777602305733 4 2.1455710973172364 2.8970123504012935 5 1.3410483196016598 2.2634029403806806 6 0.8949032287792866 1.8124864583344984 7 0.6791685377736183 1.6048078656960372 8 0.5404938098071878 1.4595808493922442 9 0.4308372082577191 1.3088900239732217 >10 2.94733985766976 19.401740663970923 >50 0.301634105803457 7.060309364457678 >100 0.18450618230653804 11.597368247049435 >500 0.012480544464751572 2.8582219513389977 >1k 0.00696404823681635 4.430907809289847 >5k 7.314458945684424E-4 1.7094603162005229 >10k+ 4.460622923134471E-4 5.0651160867991605 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA 892673 1.2073086593563158 TruSeq Adapter, Index 19 (97% over 40bp) CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 184919 0.25009640706004393 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT 166953 0.22579802750336908 TruSeq Adapter, Index 19 (97% over 40bp) GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG 98294 0.13293915841833426 No Hit AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA 90044 0.12178132521436193 No Hit AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA 89623 0.12121193760480163 No Hit ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 81097 0.10968082416273277 No Hit ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 74749 0.1010953786865126 No Hit GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA 74171 0.10031365412991915 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.4652398940224944 1.3524646307845268E-6 0.0 1.4877110938629793E-5 0.0 2 1.4739105447704541 9.467252415491687E-6 0.0 4.05739389235358E-5 0.0 3 1.4741905049490265 9.467252415491687E-6 0.0 7.168062543157992E-5 0.0 4 1.476082602967494 9.467252415491687E-6 0.0 8.250034247785613E-5 0.0 5 1.4763639156106971 9.467252415491687E-6 0.0 1.1360702898590024E-4 0.0 6 1.4767331384549014 1.3524646307845266E-5 0.0 1.6364822032492772E-4 0.0 7 1.4771037137637364 1.4877110938629793E-5 0.0 1.7446793737120394E-4 1.3524646307845268E-6 8 1.4792757719607763 2.9754221877259587E-5 0.0 1.866401190482647E-4 1.3524646307845268E-6 9 1.4798181102777208 3.1106686508044114E-5 0.0 1.879925836790492E-4 1.3524646307845268E-6 10-11 1.4811259435756896 3.9221474292751276E-5 0.0 1.9475490683297183E-4 1.3524646307845268E-6 12-13 1.4829936972308029 4.733626207745844E-5 0.0 2.3465261344111538E-4 1.3524646307845268E-6 14-15 1.4856242409376788 4.8688726708242965E-5 0.0 2.447960981719993E-4 1.3524646307845268E-6 16-17 1.489153497391711 4.8688726708242965E-5 0.0 3.083619358188721E-4 1.3524646307845268E-6 18-19 1.4944003839268398 4.8688726708242965E-5 0.0 3.442022485346621E-4 1.3524646307845268E-6 20-21 1.502828943505889 5.004119133902749E-5 0.0 3.983008337660431E-4 1.3524646307845268E-6 22-23 1.5128283907535942 5.274612060059654E-5 0.0 4.7268638845919205E-4 1.3524646307845268E-6 24-25 1.5192485403559284 5.4098585231381066E-5 0.0 4.916208932901755E-4 1.3524646307845268E-6 26-27 1.5254340373448214 5.4098585231381066E-5 0.0 5.200226505366506E-4 2.02869694617679E-6 28-29 1.5345672309965095 5.6127282177557856E-5 0.0 5.531580339908715E-4 2.7049292615690536E-6 30-31 1.5455127272534486 5.680351449295012E-5 0.0 8.926266563177876E-4 2.7049292615690536E-6 32-33 1.5579195615439505 5.680351449295012E-5 0.0 0.0010522174827503616 2.7049292615690536E-6 34-35 1.569712376892076 5.815597912373465E-5 0.0 0.001417382933062184 2.7049292615690536E-6 36-37 1.5826574921056302 5.9508443754519174E-5 0.0 0.0015032644371170015 2.7049292615690536E-6 38-39 1.5977482924559239 5.9508443754519174E-5 0.0 0.001530313729732692 2.7049292615690536E-6 40-41 1.6163041071902877 6.28896053314805E-5 0.0 0.0015648015778176975 2.7049292615690536E-6 42-43 1.637717679689499 6.424206996226501E-5 0.0 0.0015850885472794654 2.7049292615690536E-6 44-45 1.6578085417798032 6.559453459304955E-5 0.0 0.00160740421368741 2.7049292615690536E-6 46-47 1.6794527094985634 6.627076690844181E-5 0.0 0.0016966668793191887 2.7049292615690536E-6 48-49 1.703907974952409 6.627076690844181E-5 0.0 0.0017859295449509675 2.7049292615690536E-6 50-51 1.7314137243809895 7.032816080079539E-5 0.0 0.001871811049005785 2.7049292615690536E-6 52-53 1.7603355042780011 7.235685774697217E-5 0.0 0.0020009714212457074 2.7049292615690536E-6 54-55 1.7896068962820708 7.438555469314897E-5 0.0 0.0020516888449001267 2.7049292615690536E-6 56-57 1.824127203518215 7.776671627011028E-5 0.0 0.0020726520466772874 2.7049292615690536E-6 58-59 1.861817687848918 7.911918090089482E-5 0.0 0.002092939016139055 2.7049292615690536E-6 60-61 1.9008268251946363 7.979541321628708E-5 0.0 0.0021375703489549445 4.05739389235358E-6 62-63 1.9441611444296032 7.979541321628708E-5 0.0 0.0021936976311325023 4.05739389235358E-6 64-65 1.9900401258673912 7.979541321628708E-5 0.0 0.0022295379438482922 4.05739389235358E-6 66-67 2.045909087300469 7.979541321628708E-5 0.0 0.0022484724486792756 4.05739389235358E-6 68-69 2.1038574631033784 8.047164553167934E-5 0.0 0.0022971611753875186 4.05739389235358E-6 70-71 2.1727831180820503 8.11478778470716E-5 0.0 0.002354640922195861 4.05739389235358E-6 72-73 2.249574707353365 8.11478778470716E-5 0.0 0.002399948487327143 4.733626207745844E-6 74-75 2.3342119439478606 8.11478778470716E-5 0.0 0.0024553995371893083 5.409858523138107E-6 76-77 2.4305892497698816 8.182411016246388E-5 0.0 0.00250070710232059 5.409858523138107E-6 78-79 2.5406724321602727 8.385280710864066E-5 0.0 0.002549395829028833 5.409858523138107E-6 80-81 2.67877124314505 8.520527173942519E-5 0.0 0.0026460970501299263 5.409858523138107E-6 82-83 2.8267038244602616 8.655773637020971E-5 0.0 0.0028029829473009316 5.409858523138107E-6 84-85 3.0033695168564902 8.655773637020971E-5 0.0 0.0030619799240961683 5.409858523138107E-6 86-87 3.2196211727634667 8.85864333163865E-5 0.0 0.0031823492762359912 5.409858523138107E-6 88-89 3.4774286569159285 8.993889794717104E-5 0.0 0.003304747325321991 5.409858523138107E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 47225 0.0 58.17133 1 GCGTGTT 59735 0.0 44.007343 2 TGCCGTC 154735 0.0 39.92963 50-51 TATGCCG 156460 0.0 39.718594 48-49 CCGTCTT 154895 0.0 39.706375 52-53 CTGGAAT 197545 0.0 39.28542 1 CTCGTAT 151515 0.0 38.04876 44-45 CGTATGC 165985 0.0 37.530148 46-47 CGATCTC 149975 0.0 37.133827 40-41 AACGATC 152420 0.0 36.571037 38-39 ATCTCGT 155100 0.0 36.54149 42-43 GAAACGA 173195 0.0 35.378807 36-37 GCCGTCT 154485 0.0 33.946053 52-53 TGCTTGA 180530 0.0 33.906384 60-61 ATGCCGT 155745 0.0 33.870804 50-51 CGTCTTC 157770 0.0 33.294212 54-55 AGTCACG 189420 0.0 32.881214 28-29 TCACGTG 190645 0.0 32.691154 30-31 TCTCGTA 148345 0.0 32.63273 44-45 GTTTTTT 835605 0.0 32.61459 1 >>END_MODULE