FastQCFastQC Report
Fri 27 May 2016
SRR1233062_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233062_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70004595
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1510020.21570298349701186No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG1458680.20836917919459427No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA1160550.1657819747403724No Hit
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA1019310.14560615628159837No Hit
GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT812660.11608666545388914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT568900.068.569511
GCGTGTT712450.050.8541152
GTTTTTT10046750.036.0284581
CGTGTTT1111750.032.0704153
CGGCGAG83300.031.2548981
CGCGTGC36900.030.7283211
CTGGAAT2242600.028.422511
CGCGTTT52550.024.7256581
CTGGCGA82750.024.6996361
CGCGAGG32800.022.1065881
CGGCAAG110350.020.4623221
CGCGTAT36350.017.9394721
GGAAAAC3746350.017.9154681
TCCGATT208400.017.6491262
CCGAAAC116900.017.484131
CCCGTAT48100.016.9970471
ATCCGAT191250.016.793911
CGCGTCT64650.016.4848441
TATGGCG3477800.016.3852487
CCGATTA217750.016.1480163
Produced by FastQC (version 0.11.5)