FastQCFastQC Report
Fri 27 May 2016
SRR1233062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233062_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70004595
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1539610.21992984889063352No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG1265810.1808181305812854No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA1129550.16135369399680122No Hit
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA968830.13839520105787342No Hit
GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT758780.10839002782603056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT525500.065.392721
GCGTGTT623650.051.0962072
CGCGTTT74100.041.7214551
GCGCGTT33000.034.0143621
CGTGTTT966950.033.1665153
GCGGGTT55100.032.5427321
GTAAACT479150.032.2913862
CGGCGAG81950.028.4968471
GGTAAAC274000.028.0861531
CGCGTGC37150.027.9101351
CCGGAAT80400.026.9757211
CTGGAAT2320400.026.2983251
CGGGAAT90350.026.2685851
CGGGTTT161900.025.5292021
TAAACTG680700.024.272013
CTGGCGA74150.023.8614081
GTCGTTT110650.023.555561
GTTTTTT7690750.023.3899121
GATAACT412750.022.620572
CGGCAAG107250.022.0849461
Produced by FastQC (version 0.11.5)