FastQCFastQC Report
Fri 27 May 2016
SRR1233061_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233061_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67554522
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA4286720.6345570767268548No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT2814720.41665900618762425No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT2383800.3528705302659088No Hit
ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA2192460.3245467416674194No Hit
CTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTCACT1901200.2814319373024355No Hit
GTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGT1586930.23491099529947085No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC1427280.21127823241795718No Hit
TTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACAA1411940.20900747399263667No Hit
CGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTC1402340.2075863996195547No Hit
ATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACA1289700.19091246030872663No Hit
GTCGTATTACAATTTCCCTATAGTGAGTCGTATTACAATTTCCCTATAGT1122400.16614727878616328No Hit
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG1016550.1504784535371296No Hit
GGAAATTGTAATACGACTCACTATAGGGAAATTGTAATACGACTCACTAT938900.13898403425902414No Hit
CTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACGACT915240.1354816780436993No Hit
ATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAAT897700.13288525674121415No Hit
ATTTAATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTT856700.12681608493951005No Hit
ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT851970.12611590975360612No Hit
ATACGACTCACTATAGGGAAATTGTAATACGACTCACTATAGGGAAATTG753760.11157802285981684No Hit
GTCGTATTACACCTATAGTGAGTCGTATTACACCTATAGTGAGTCGTATT736830.10907189899145464No Hit
CTCACTATAGGGAAATTGTAATACGACTCACTATAGGGAAATTGTAATAC709190.1049803890256229No Hit
CCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCC700000.103620006370558No Hit
ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT699460.10354007093707214No Hit
GGGAAATTGTAATACGACTCACTATAGGGAAATTGTAATACGACTCACTA683790.10122046308017693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA61700.031.8001961
GTTTTTT4305850.031.4061151
CTCGTAT72600.030.6223721
CGCGTGT530800.021.0542641
CCGATCT39350.020.7477282
TACGACG92800.019.70261854-55
GCGTGTT594400.018.1380542
TCCGATC44400.017.6427171
CCGTCGT18150.017.469141
CGGACGT16100.017.1449031
CGGTCGT17800.016.764831
CTGGAAT791800.016.6863821
CGACTCG77700.016.05760454-55
CGGGTCG13900.015.8331161
ATACGAG108250.015.20384554-55
CGTGTTT713350.015.0647943
TATGGCG740250.014.9449287
ACTCACG93900.014.90768254-55
TGGCGAG747400.014.7619639
CCCGCGT26750.014.5023621
Produced by FastQC (version 0.11.5)