FastQCFastQC Report
Fri 27 May 2016
SRR1233061_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233061_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67554522
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA3784730.5602482095869171No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT2339610.34632914729231595No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT2176420.32217236323572834No Hit
ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA2128460.3150729125135398No Hit
CTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTCACT1617220.23939478100370543No Hit
TTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACAA1445190.21392942429523817No Hit
GTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGT1413700.20926800429436834No Hit
CGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTC1285170.1902418908389286No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC1256210.18595498314679806No Hit
ATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACA1192540.17653000342449318No Hit
GTCGTATTACAATTTCCCTATAGTGAGTCGTATTACAATTTCCCTATAGT1001230.14821065568341968No Hit
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG912760.13511456716398643No Hit
ATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAAT889380.1316536589512098No Hit
GGAAATTGTAATACGACTCACTATAGGGAAATTGTAATACGACTCACTAT861030.1274570486932022No Hit
CTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACGACT781780.11572578368624975No Hit
ATACGACTCACTATAGGGAAATTGTAATACGACTCACTATAGGGAAATTG727510.10769227262092092No Hit
ATTTAATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTT710110.1051165753197099No Hit
ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT694890.10286358032405292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAC135900.055.673371
TTCGACT166500.046.602692
TTCGTAT137500.046.0968631
CCGATAG81100.044.885951
CGATAGT88250.044.797892
CGATTCA107600.037.899931
CGTCTCA155750.036.9069941
CGTCGTA59100.033.0115741
TCGACTC270100.030.0662943
GTTGTAT345400.029.881821
CGCACTA86150.028.770922
GTTTTTT3773250.026.6044561
CGCGTGT606450.021.5307391
GATAGTG206050.021.1261043
TGTATTA528300.020.5046983
GTACGAC57400.020.2277581
GCGTGTT630550.020.1221682
GTCTTAT296800.020.0729061
ATATGAC286200.020.0349031
TATGACT302200.019.9144062
Produced by FastQC (version 0.11.5)