##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233061_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 67554522 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.759786073240218 31.0 31.0 34.0 27.0 34.0 2 31.377059584553052 31.0 31.0 34.0 28.0 34.0 3 29.588508138655765 31.0 30.0 34.0 16.0 34.0 4 32.198508443298586 35.0 32.0 37.0 19.0 37.0 5 34.128376098938276 35.0 33.0 37.0 28.0 37.0 6 34.995053758207334 36.0 35.0 37.0 32.0 37.0 7 35.39655163276856 37.0 35.0 37.0 33.0 37.0 8 35.52227890828685 37.0 35.0 37.0 33.0 37.0 9 37.208809989063354 39.0 37.0 39.0 33.0 39.0 10-11 37.163349138936994 39.0 37.0 39.0 33.0 39.0 12-13 37.16609947295608 39.0 37.0 39.0 33.0 39.0 14-15 38.397111647093 40.0 38.0 41.0 33.0 41.0 16-17 38.29951066784248 40.0 38.0 41.0 33.0 41.0 18-19 38.21589069936725 40.0 37.0 41.0 33.0 41.0 20-21 38.08440323950482 40.0 37.0 41.0 33.0 41.0 22-23 37.97729226771821 40.0 37.0 41.0 33.0 41.0 24-25 37.80966814479126 40.0 37.0 41.0 33.0 41.0 26-27 37.64581732219199 40.0 37.0 41.0 32.0 41.0 28-29 37.62739772623955 40.0 37.0 41.0 32.0 41.0 30-31 37.56323826849075 40.0 37.0 41.0 32.0 41.0 32-33 37.47154416990768 40.0 37.0 41.0 32.0 41.0 34-35 37.33253596258145 40.0 37.0 41.0 31.5 41.0 36-37 37.215354473235706 40.0 37.0 41.0 31.0 41.0 38-39 37.10473190084892 40.0 37.0 41.0 31.0 41.0 40-41 37.135565558438856 40.0 37.0 41.0 31.0 41.0 42-43 37.124254568776315 40.0 37.0 41.0 31.0 41.0 44-45 37.03188540805603 40.0 37.0 41.0 31.0 41.0 46-47 36.91459602807936 40.0 36.5 41.0 31.0 41.0 48-49 36.83844162201311 40.0 36.0 41.0 30.0 41.0 50-51 36.75614188344046 40.0 36.0 41.0 30.0 41.0 52-53 36.62887376362459 39.0 36.0 41.0 30.0 41.0 54-55 36.47467480415301 39.0 36.0 41.0 30.0 41.0 56-57 36.30367951534022 39.0 35.5 41.0 29.5 41.0 58-59 36.07263345005979 39.0 35.0 40.0 29.0 41.0 60-61 35.83734259861983 39.0 35.0 40.0 28.0 41.0 62-63 35.5727131634504 38.0 34.5 40.0 28.0 41.0 64-65 35.280552802964095 38.0 34.0 40.0 28.0 41.0 66-67 34.958024046117885 38.0 34.0 40.0 27.5 41.0 68-69 34.61449847872508 37.0 34.0 39.5 26.5 41.0 70-71 34.29137737811246 37.0 33.5 39.0 26.0 41.0 72-73 33.91198754244756 36.0 33.0 39.0 26.0 40.0 74-75 33.49831373982633 36.0 33.0 38.5 26.0 40.0 76-77 32.61934637032884 35.0 31.5 37.0 25.5 39.0 78-79 32.76488600570662 35.0 32.0 37.0 26.0 39.0 80-81 32.50905301350515 35.0 32.0 37.0 26.0 39.0 82-83 32.14065851875911 35.0 32.0 36.0 25.0 38.5 84-85 31.735217569891176 35.0 32.0 36.0 24.5 37.0 86-87 31.363418676842983 34.0 31.0 35.0 23.5 37.0 88-89 31.053609046334454 34.0 31.0 35.0 22.5 36.5 90-91 30.70690409888475 34.0 31.0 35.0 20.5 36.0 92-93 30.36835265446775 34.0 31.0 35.0 19.5 36.0 94-95 30.015832648479105 34.0 31.0 35.0 17.5 35.5 96-97 29.61357370717537 34.0 30.0 35.0 4.5 35.0 98-99 29.166902957288336 34.0 30.0 35.0 2.0 35.0 100-101 27.50530871197638 32.5 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.8132352451860712 1101 2 1.416509097875494 1101 3 3.3024445357861687 1101 4 4.071634716027862 1101 5 1.9201702344546874 1101 6 1.0101302986114504 1101 7 0.6682016864362552 1101 8 0.4813140914220071 1101 9 0.5228339031236047 1101 10-11 0.4544921651670961 1101 12-13 0.44386732808024476 1101 14-15 0.5584290851057929 1101 16-17 0.5600476514131572 1101 18-19 0.5123520608625682 1101 20-21 0.5305561486711952 1101 22-23 0.5311033754087049 1101 24-25 0.5419000487710122 1101 26-27 0.5797305539310997 1101 28-29 0.5338283578786118 1101 30-31 0.5249691058921968 1101 32-33 0.5087705723919882 1101 34-35 0.5210900767807018 1101 36-37 0.5624193983395216 1101 38-39 0.5951345552858882 1101 40-41 0.5336351029320028 1101 42-43 0.5295028523036152 1101 44-45 0.4342619422713412 1101 46-47 0.47693249543026894 1101 48-49 0.49767469955900623 1101 50-51 0.5611829486037863 1101 52-53 0.6408349543467864 1101 54-55 0.5971145173522387 1101 56-57 0.6034796158929225 1101 58-59 0.5785241680501372 1101 60-61 0.5364164295852802 1101 62-63 0.5500669733823926 1101 64-65 0.5677548621143629 1101 66-67 0.5351339436259508 1101 68-69 0.5187467083257218 1101 70-71 0.6408389753231489 1101 72-73 0.6625815259410643 1101 74-75 0.6427764297617173 1101 76-77 0.5228113668426175 1101 78-79 0.6061074067167951 1101 80-81 0.6284902556621148 1101 82-83 0.6467599377811482 1101 84-85 0.6495986767860273 1101 86-87 0.6504991195007115 1101 88-89 0.6982150716785043 1101 90-91 0.725084293706427 1101 92-93 0.739972065267164 1101 94-95 0.7203488639300808 1101 96-97 0.7023709607584969 1101 98-99 0.8235215847323722 1101 100-101 1.2823177919093247 1102 1 1.6666913491415727 1102 2 1.313938212600295 1102 3 3.221608374639626 1102 4 4.011394107144607 1102 5 1.8702478928385062 1102 6 0.9808722064634736 1102 7 0.6067609618412746 1102 8 0.48811024554554194 1102 9 0.49881464369549633 1102 10-11 0.4264859883655916 1102 12-13 0.3933376940354947 1102 14-15 0.5120584869100568 1102 16-17 0.5080283880107714 1102 18-19 0.44332199933176497 1102 20-21 0.4641512384831685 1102 22-23 0.4623000867590079 1102 24-25 0.4964437094928016 1102 26-27 0.5008454815161656 1102 28-29 0.443985869148392 1102 30-31 0.4214278216470504 1102 32-33 0.4233052140916058 1102 34-35 0.4534136817931298 1102 36-37 0.4757012666589091 1102 38-39 0.4942432525785634 1102 40-41 0.4749351329112699 1102 42-43 0.49357830575235795 1102 44-45 0.44467004133617394 1102 46-47 0.3766169596313276 1102 48-49 0.3909043251806352 1102 50-51 0.4842160636671409 1102 52-53 0.44851393282267793 1102 54-55 0.5077615657179848 1102 56-57 0.5164168663654394 1102 58-59 0.5124005624689829 1102 60-61 0.4553931305244632 1102 62-63 0.42514971800419943 1102 64-65 0.41222322034486325 1102 66-67 0.4129181535313222 1102 68-69 0.41780662200355323 1102 70-71 0.5077622654341312 1102 72-73 0.5593796950432974 1102 74-75 0.4969897537153969 1102 76-77 0.43744766757075837 1102 78-79 0.46023527134104114 1102 80-81 0.5448516615557821 1102 82-83 0.5496564741289944 1102 84-85 0.5477128305620838 1102 86-87 0.4619153048009963 1102 88-89 0.5410523298247796 1102 90-91 0.6479338531939725 1102 92-93 0.5883895374083998 1102 94-95 0.6834600474154477 1102 96-97 0.7324367198030366 1102 98-99 0.7801177035815066 1102 100-101 1.2363385726348177 1103 1 1.7168081627793157 1103 2 1.322991593343982 1103 3 3.195572905351259 1103 4 4.043803044246754 1103 5 1.8536071617466163 1103 6 0.9392733790698031 1103 7 0.594018081500387 1103 8 0.4259858272216235 1103 9 0.38910407424598503 1103 10-11 0.34263623397533394 1103 12-13 0.33391111582176336 1103 14-15 0.474292569222321 1103 16-17 0.42406475099603824 1103 18-19 0.4060626319033531 1103 20-21 0.427252127585362 1103 22-23 0.3987960442370664 1103 24-25 0.4069573240759965 1103 26-27 0.4623685144870109 1103 28-29 0.43977114905175085 1103 30-31 0.3923529221587856 1103 32-33 0.434434267666326 1103 34-35 0.43792821403066995 1103 36-37 0.3740503296815447 1103 38-39 0.44359051899805024 1103 40-41 0.4481466769025886 1103 42-43 0.43063262623119414 1103 44-45 0.4290840903239257 1103 46-47 0.3620620051289336 1103 48-49 0.38947915910080155 1103 50-51 0.44367337374055893 1103 52-53 0.4909768745850229 1103 54-55 0.55221566577994 1103 56-57 0.5540413554668291 1103 58-59 0.5124991489281925 1103 60-61 0.40344308398562845 1103 62-63 0.31846320421902163 1103 64-65 0.3781126200876841 1103 66-67 0.3546513877460029 1103 68-69 0.4447840002273651 1103 70-71 0.5095624520517532 1103 72-73 0.4663499008965104 1103 74-75 0.5090628286006762 1103 76-77 0.4521975454021785 1103 78-79 0.52239871241148 1103 80-81 0.49979402133787687 1103 82-83 0.5800760075600166 1103 84-85 0.5487138304262373 1103 86-87 0.5166610279577384 1103 88-89 0.5929457117329378 1103 90-91 0.6170503050412712 1103 92-93 0.6849923911120541 1103 94-95 0.7008319507222858 1103 96-97 0.7307150109961533 1103 98-99 0.7433625910555044 1103 100-101 1.2122425610262333 1104 1 1.675921456786007 1104 2 1.2789069196234557 1104 3 3.1532847498793153 1104 4 3.9809930090875447 1104 5 1.8233006806012853 1104 6 0.909930119735634 1104 7 0.5078683641868977 1104 8 0.4071758308864162 1104 9 0.3688231676648144 1104 10-11 0.28748009540395714 1104 12-13 0.24808414677049484 1104 14-15 0.33519244516786983 1104 16-17 0.3170348050833809 1104 18-19 0.29146333922547285 1104 20-21 0.3164513423541422 1104 22-23 0.282160403842596 1104 24-25 0.320010476299295 1104 26-27 0.3860340802181099 1104 28-29 0.34249095258097384 1104 30-31 0.2847736752983181 1104 32-33 0.3045882989681772 1104 34-35 0.2921834857936645 1104 36-37 0.2842565329671629 1104 38-39 0.32833613046138765 1104 40-41 0.31793909087033967 1104 42-43 0.35466201003630715 1104 44-45 0.32770306079384426 1104 46-47 0.2850015838906259 1104 48-49 0.2835694704023126 1104 50-51 0.3430761134194782 1104 52-53 0.36168409477411245 1104 54-55 0.35522618706824005 1104 56-57 0.39872624170329374 1104 58-59 0.4266590674790507 1104 60-61 0.371052705154824 1104 62-63 0.3213852074713017 1104 64-65 0.30137133323241727 1104 66-67 0.28095614582345974 1104 68-69 0.4146336484346449 1104 70-71 0.41917524875410095 1104 72-73 0.41298518534125606 1104 74-75 0.4457734552746757 1104 76-77 0.3323426301715813 1104 78-79 0.31168442773373073 1104 80-81 0.3544655975830011 1104 82-83 0.40165303762198334 1104 84-85 0.4105453088045863 1104 86-87 0.4687615565497083 1104 88-89 0.5184893844630523 1104 90-91 0.4951873596744356 1104 92-93 0.4421137217601654 1104 94-95 0.4624116015915938 1104 96-97 0.5453840260975191 1104 98-99 0.5796730056074253 1104 100-101 1.0487516891275526 1105 1 1.721999263169117 1105 2 1.3733472857923381 1105 3 3.065732079589619 1105 4 3.9860161664838465 1105 5 1.898089792313769 1105 6 0.9799135516713307 1105 7 0.6372568009981379 1105 8 0.5003238446796914 1105 9 0.46405343526157594 1105 10-11 0.4437471823700676 1105 12-13 0.44199143212001957 1105 14-15 0.5237032030477664 1105 16-17 0.49834549620939583 1105 18-19 0.4641908048576937 1105 20-21 0.5082104747445939 1105 22-23 0.5384035425831684 1105 24-25 0.5438873524561103 1105 26-27 0.5417099891331887 1105 28-29 0.5052021890030431 1105 30-31 0.5026469878498503 1105 32-33 0.49915933818117963 1105 34-35 0.5213699206634672 1105 36-37 0.5158167999053589 1105 38-39 0.587698104898216 1105 40-41 0.5859787240671039 1105 42-43 0.5849102777297972 1105 44-45 0.5436614354580058 1105 46-47 0.5261900369119061 1105 48-49 0.5483804736039488 1105 50-51 0.5449148544370885 1105 52-53 0.6262861095285359 1105 54-55 0.5913169981445066 1105 56-57 0.5546677899733865 1105 58-59 0.6258567952234557 1105 60-61 0.5768955434901031 1105 62-63 0.6130246301661586 1105 64-65 0.5506377409056213 1105 66-67 0.6163434778010526 1105 68-69 0.7000375028749914 1105 70-71 0.7192364420337185 1105 72-73 0.7303710557739009 1105 74-75 0.705483011888191 1105 76-77 0.6078033459065821 1105 78-79 0.6464469041896947 1105 80-81 0.6202075746976234 1105 82-83 0.6910027721217276 1105 84-85 0.7224543886231629 1105 86-87 0.6577386121802817 1105 88-89 0.7338752482746322 1105 90-91 0.791886778536103 1105 92-93 0.8441026749593021 1105 94-95 0.7751427709348384 1105 96-97 0.921497439815095 1105 98-99 0.9827264777952429 1105 100-101 1.3829051733139117 1106 1 1.6491972966015283 1106 2 1.2456889666872222 1106 3 3.1261019671967 1106 4 3.971854699531651 1106 5 1.7842860609289986 1106 6 0.8906109245043226 1106 7 0.4796958764378587 1106 8 0.3222711006887806 1106 9 0.309567278036063 1106 10-11 0.2507748723204344 1106 12-13 0.17854255592165202 1106 14-15 0.24443474436302637 1106 16-17 0.2830465847367094 1106 18-19 0.2828100912701075 1106 20-21 0.3074157182161059 1106 22-23 0.25379859301701657 1106 24-25 0.24146065633156866 1106 26-27 0.2938877957727897 1106 28-29 0.28402789332449174 1106 30-31 0.23734190397447463 1106 32-33 0.25100088244099794 1106 34-35 0.2763243798258088 1106 36-37 0.32957701731280764 1106 38-39 0.29044982752736104 1106 40-41 0.264041770371918 1106 42-43 0.2699947590824934 1106 44-45 0.23167135909685754 1106 46-47 0.24121499725161755 1106 48-49 0.3316522852361956 1106 50-51 0.3632901797896224 1106 52-53 0.3630731377868557 1106 54-55 0.3835223109866064 1106 56-57 0.37524597825369455 1106 58-59 0.3904157703989455 1106 60-61 0.3042442215998662 1106 62-63 0.3232587426924809 1106 64-65 0.32601370971374166 1106 66-67 0.28288043693960674 1106 68-69 0.4136833855995974 1106 70-71 0.4119716704720986 1106 72-73 0.4076008679132741 1106 74-75 0.4182735992867208 1106 76-77 0.31664776733432376 1106 78-79 0.42285167036362736 1106 80-81 0.4228838733959961 1106 82-83 0.4653499609923202 1106 84-85 0.5132516717679891 1106 86-87 0.4598461331018342 1106 88-89 0.4344296071734348 1106 90-91 0.42667797264540397 1106 92-93 0.4969544444470593 1106 94-95 0.533014372221853 1106 96-97 0.5960594365285736 1106 98-99 0.6978287518187543 1106 100-101 1.1520515862518543 1107 1 0.8967384374156673 1107 2 0.6992578919104204 1107 3 1.1162726043830027 1107 4 -1.9458449122101484 1107 5 -0.4428007752709249 1107 6 -0.08832850649667279 1107 7 0.00980684314983904 1107 8 0.05004821702567597 1107 9 0.17685553738931503 1107 10-11 0.10498889602374106 1107 12-13 0.1339457478185082 1107 14-15 0.14501914449897413 1107 16-17 0.17328588105758058 1107 18-19 0.1689393867499973 1107 20-21 0.24657151774590602 1107 22-23 0.24369828920585945 1107 24-25 0.25127472780697246 1107 26-27 0.23283252830890433 1107 28-29 0.22499322527390575 1107 30-31 0.1781520476953915 1107 32-33 0.17601159362813945 1107 34-35 0.09466053720867507 1107 36-37 0.05108049478408816 1107 38-39 0.16556501125538858 1107 40-41 0.13527610529632028 1107 42-43 0.14863118454774593 1107 44-45 0.1758295784998083 1107 46-47 0.1970853132258341 1107 48-49 0.19454605781911738 1107 50-51 0.2361469528019171 1107 52-53 0.1521972827163296 1107 54-55 0.21000410883668508 1107 56-57 0.24194240190318794 1107 58-59 0.18487080909372366 1107 60-61 0.17206541507594153 1107 62-63 0.21013287769050493 1107 64-65 0.19964630434795794 1107 66-67 0.14908829768580745 1107 68-69 0.22762534068866103 1107 70-71 0.22924181416331635 1107 72-73 0.2514911873438308 1107 74-75 0.27603138881750056 1107 76-77 0.18872600973832476 1107 78-79 0.2520793025204995 1107 80-81 0.34158067528379377 1107 82-83 0.34832458677455236 1107 84-85 0.3176807587230144 1107 86-87 0.3592412089468553 1107 88-89 0.39114046357468624 1107 90-91 0.37419257300366837 1107 92-93 0.3542214618774828 1107 94-95 0.4091896484520241 1107 96-97 0.35930625521941195 1107 98-99 0.45386644214513083 1107 100-101 0.9105522382196689 1108 1 0.6114898948485568 1108 2 0.5597317916007185 1108 3 -1.9749730439453792 1108 4 -2.189215562906959 1108 5 -0.7956320311076013 1108 6 -0.34882891904391045 1108 7 -0.12217064030322433 1108 8 -0.06449570295522733 1108 9 0.0301800166232411 1108 10-11 0.03472166769461893 1108 12-13 0.017219331337528843 1108 14-15 0.0366543706238005 1108 16-17 0.05644892849667826 1108 18-19 0.045236209237117464 1108 20-21 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-1.6546694680926066 2316 70-71 -1.6798480497885322 2316 72-73 -1.5773215228000197 2316 74-75 -1.6706872158465806 2316 76-77 -1.6774835082649489 2316 78-79 -1.7542348074705352 2316 80-81 -1.6757527655238462 2316 82-83 -1.7124739019709985 2316 84-85 -1.7105461665544865 2316 86-87 -1.7560986995364374 2316 88-89 -1.820271487461305 2316 90-91 -1.777426640938259 2316 92-93 -1.8731442829257752 2316 94-95 -1.9137953912064596 2316 96-97 -1.9982092642197493 2316 98-99 -2.1065900875626262 2316 100-101 -2.4523097598990304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7212.0 3 688.0 4 2087.0 5 5477.0 6 15664.0 7 46380.0 8 123178.0 9 230107.0 10 312684.0 11 259616.0 12 187162.0 13 147810.0 14 137161.0 15 140964.0 16 150470.0 17 163037.0 18 177543.0 19 196398.0 20 217566.0 21 244894.0 22 282844.0 23 334260.0 24 405253.0 25 504561.0 26 636524.0 27 808097.0 28 1019581.0 29 1287198.0 30 1614098.0 31 2007380.0 32 2498751.0 33 3150189.0 34 4089515.0 35 5549106.0 36 8164926.0 37 1.3100763E7 38 1.6279843E7 39 3053753.0 40 1782.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.560273827360465 21.29313415445 12.590693423813141 37.555898594376394 2 16.8393600645689 19.48947799971232 50.41679753013651 13.254364405582262 3 19.35892415854216 26.258341817206375 32.135010754299095 22.247723269952374 4 23.000004151608437 32.42220997827226 25.62424325815858 18.953542611960724 5 19.833433621406748 33.43428458257278 30.86528354341475 15.866998252605717 6 14.397674812674813 40.64806407658917 28.385604992691526 16.56865611804449 7 12.314353595985464 19.93642614481641 49.08892227756848 18.660297981629647 8 14.483635899311079 21.819343196596076 41.8970102401139 21.80001066397894 9 16.7979073258782 24.46242458794986 35.492131822056265 23.247536264115674 10-11 20.574512696196898 35.287701173989525 25.14151421486654 18.996271914947037 12-13 18.282969273322667 27.35966291050065 35.555677531105914 18.801690285070777 14-15 19.545504296514746 27.698498110903664 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19.562534459337854 40-41 20.879243247088798 28.930332186507897 30.67218110394705 19.518243462456258 42-43 21.138737593930387 29.731341961929143 30.12497164603442 19.00494879810605 44-45 20.918130544043006 30.258961396317314 29.909149911210047 18.913758148429636 46-47 20.458551899001918 29.460464636502525 30.396364355391235 19.68461910910432 48-49 20.151455519516745 30.40133862313736 29.53284635606184 19.914359501284057 50-51 18.979135845265844 30.048093597642513 31.217321025526612 19.755449531565038 52-53 19.282949650872347 30.10795858402777 30.48462251606358 20.1244692490363 54-55 19.11469713857429 30.869076313638903 30.023664551268396 19.992561996518408 56-57 19.645272537587207 30.213136753976677 30.22219685994535 19.919393848490767 58-59 20.218231337554723 29.493402174913296 29.999765592708975 20.288600894823002 60-61 20.770753427233977 30.164212608675268 29.328248210094245 19.736785753996507 62-63 20.025917203512556 30.46815821711739 29.827439072195766 19.67848550717429 64-65 20.82769403566322 28.863118774120615 30.447946484323552 19.86124070589261 66-67 20.199563574734412 30.913888599895355 29.042583607371377 19.84396421799886 68-69 20.625328634155114 29.898623806242284 30.149291332978546 19.326756226624056 70-71 20.97215321293228 29.4630022165647 29.421015258530392 20.143829311972624 72-73 19.081666411144553 32.2285758254451 28.835595731582202 19.854162031828142 74-75 19.20758209713442 29.539571146268333 31.398218424065625 19.85462833253162 76-77 19.997438216854455 30.450303923462812 29.6512824873212 19.900975372361533 78-79 19.501146610957587 31.270893911721004 29.27830030019828 19.949659177123127 80-81 19.704690670373882 29.928936736889373 30.391060701618855 19.97531189111789 82-83 20.397655101170137 30.672351057267548 29.185694459808587 19.744299381753727 84-85 19.9830110381401 30.42955503827901 29.92848369141151 19.658950232169378 86-87 20.62333739849421 29.857874651233562 29.440517690288743 20.078270259983483 88-89 20.35875999537085 30.667210553277247 29.165240781364716 19.808788669987184 90-91 19.787795739641933 30.45861268338968 30.023737456637505 19.72985412033088 92-93 20.48068791540339 30.617679931381602 29.059863905306305 19.841768247908703 94-95 19.80083984226625 30.904438381591305 29.369906854219845 19.924814921922604 96-97 19.51507745148735 30.869893016958887 29.28028887353708 20.33474065801668 98-99 19.516885310979777 31.025116405341908 29.716175578768222 19.741822704910096 100-101 19.726380484776392 31.5718304657434 28.97052025915321 19.731268790326993 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2793.0 1 3642.5 2 4053.0 3 4097.5 4 5500.5 5 7381.0 6 9342.5 7 11754.5 8 14721.5 9 18071.0 10 21562.5 11 25277.5 12 29421.0 13 33613.0 14 38090.5 15 43485.5 16 49223.0 17 56009.0 18 65385.0 19 76679.5 20 91664.5 21 114038.0 22 144489.0 23 184407.0 24 241177.0 25 315792.5 26 399875.0 27 518191.5 28 782987.0 29 1099387.0 30 1544241.0 31 1989635.0 32 3003736.0 33 3689457.0 34 3427783.5 35 3610498.0 36 3781336.0 37 3605872.0 38 3411480.0 39 3103400.5 40 3084767.0 41 2871221.5 42 2396081.0 43 2281746.0 44 2166041.0 45 2056914.0 46 1953530.5 47 1826525.0 48 1705442.0 49 1589561.5 50 1448973.0 51 1309624.0 52 1187318.0 53 1075443.5 54 955703.5 55 825008.5 56 707296.5 57 591918.0 58 485058.5 59 388564.0 60 295229.0 61 220634.0 62 164875.5 63 118710.0 64 82182.5 65 55704.5 66 37888.0 67 26602.0 68 18381.5 69 12242.0 70 8172.5 71 5954.0 72 4944.5 73 4490.5 74 3657.5 75 2456.5 76 1758.0 77 1501.0 78 1287.5 79 897.0 80 593.5 81 418.0 82 313.0 83 232.0 84 167.0 85 115.0 86 72.0 87 41.0 88 33.0 89 28.5 90 17.5 91 11.5 92 8.0 93 4.5 94 2.0 95 1.0 96 1.5 97 1.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022253136510979973 2 2.8125430300580028E-5 3 0.007093529578967342 4 0.16398902208204508 5 0.0011738666435978928 6 0.0010939312101120337 7 3.256628771646108E-5 8 0.0 9 0.0 10-11 1.4802858052936855E-6 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.810928867204479E-5 22-23 1.5468986665319014E-4 24-25 5.1069860282632154E-5 26-27 5.773114640645374E-5 28-29 7.57906332310367E-4 30-31 2.1464144176758443E-5 32-33 7.993543348585902E-5 34-35 6.609476120636306E-4 36-37 2.701521594660976E-4 38-39 3.90795452597533E-4 40-41 3.1530087652755504E-4 42-43 5.6991003503806895E-5 44-45 0.0010480423501479294 46-47 0.005469656050560168 48-49 5.1069860282632154E-5 50-51 0.0 52-53 7.401429026468428E-6 54-55 1.5320958084789646E-4 56-57 1.8429558275906386E-4 58-59 0.0017208322486539096 60-61 0.005357154329357848 62-63 0.006528060401345153 64-65 0.01576504382637775 66-67 0.010176224768491442 68-69 4.42605455782812E-4 70-71 3.130804478196145E-4 72-73 4.2928288353516886E-5 74-75 3.4120587812019453E-4 76-77 2.0724001274111598E-5 78-79 6.661286123821585E-6 80-81 2.0353929822788177E-4 82-83 7.549457606997797E-5 84-85 1.0436014927320484E-4 86-87 0.0 88-89 0.0 90-91 4.699907431807452E-4 92-93 0.005242432179447588 94-95 0.028981775638942425 96-97 0.004887163586177103 98-99 2.5090844399727974E-4 100-101 0.05364333715513523 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6.7554522E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.94183342594699 #Duplication Level Percentage of deduplicated Percentage of total 1 70.48456916614964 19.694680907401928 2 11.5308327940257 6.443852183862261 3 5.186832624313489 4.3478883959050485 4 2.94167426572505 3.28783089305137 5 1.9081065826820103 2.665799814612684 6 1.386956782451886 2.32524692305548 7 0.9846234798472846 1.925852968281837 8 0.7546805712008119 1.686972704823329 9 0.594440192046296 1.4948773945060987 >10 3.744836447740381 19.556653355554886 >50 0.29359690374889863 5.644664533218876 >100 0.16356688217984053 8.520203392325481 >500 0.012906220659989346 2.498620973092298 >1k 0.009776677329075747 5.486475479893903 >5k 0.001187507891140168 2.302857885764511 >10k+ 0.0014129020084556827 12.117522194650114 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA 378473 0.5602482095869171 No Hit ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 233961 0.34632914729231595 No Hit ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 217642 0.32217236323572834 No Hit ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA 212846 0.3150729125135398 No Hit CTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTCACT 161722 0.23939478100370543 No Hit TTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACAA 144519 0.21392942429523817 No Hit GTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGT 141370 0.20926800429436834 No Hit CGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTC 128517 0.1902418908389286 No Hit GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC 125621 0.18595498314679806 No Hit ATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACA 119254 0.17653000342449318 No Hit GTCGTATTACAATTTCCCTATAGTGAGTCGTATTACAATTTCCCTATAGT 100123 0.14821065568341968 No Hit CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG 91276 0.13511456716398643 No Hit ATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAAT 88938 0.1316536589512098 No Hit GGAAATTGTAATACGACTCACTATAGGGAAATTGTAATACGACTCACTAT 86103 0.1274570486932022 No Hit CTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACGACT 78178 0.11572578368624975 No Hit ATACGACTCACTATAGGGAAATTGTAATACGACTCACTATAGGGAAATTG 72751 0.10769227262092092 No Hit ATTTAATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTT 71011 0.1051165753197099 No Hit ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT 69489 0.10286358032405292 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.006202397524180543 1.332257224764317E-5 0.0 1.7763429663524228E-5 0.0 2 0.006285293529276989 3.5526859327048455E-5 0.0 3.996771674292951E-5 0.0 3 0.006479210969770462 5.921143221174742E-5 0.0 4.2928288353516886E-5 0.0 4 0.00718530729889555 6.0691718017041106E-5 0.0 5.4770574795866366E-5 0.0 5 0.00737034302455726 6.0691718017041106E-5 0.0 6.365228962762849E-5 0.0 6 0.007701927044943047 6.0691718017041106E-5 0.0 7.105371865409691E-5 0.0 7 0.007814428766145366 7.25340044593906E-5 0.0 7.697486187527166E-5 0.0 8 0.008042392780160593 7.993543348585902E-5 0.0 9.029743412291481E-5 0.0 9 0.008443550233395183 9.17777199282085E-5 0.0 1.0213972056526431E-4 0.0 10-11 0.009524158871259573 1.4062715150290012E-4 0.0 1.0658057798114537E-4 0.0 12-13 0.011381177414000503 1.7171315341406752E-4 0.0 1.1694257861820116E-4 0.0 14-15 0.013622330123215141 1.865160114670044E-4 0.0 1.228637218393759E-4 0.0 16-17 0.016161020279293812 1.9243715468817912E-4 0.0 1.3766657989231276E-4 0.0 18-19 0.018928414592290357 2.0057872661729439E-4 0.0 1.4802858052936858E-4 0.0 20-21 0.021584787469889878 2.146414417675844E-4 0.0 1.5320958084789646E-4 0.0 22-23 0.02427742734971909 2.2204287079405285E-4 0.0 1.6579201019289278E-4 0.0 24-25 0.02694268194215037 2.368457288469897E-4 0.0 1.7615401082994857E-4 0.0 26-27 0.029574630103962544 2.516485868999266E-4 0.0 1.8059486824582963E-4 0.0 28-29 0.03237459070467555 2.575697301211013E-4 0.0 1.8873644017494492E-4 0.0 30-31 0.03555424461444638 2.723725881740381E-4 0.0 1.9687801210406018E-4 0.0 32-33 0.03914171726357564 2.9975787557197137E-4 0.0 2.0279915532523491E-4 0.0 34-35 0.043111103650470654 3.2566287716461086E-4 0.0 2.1908229918346546E-4 0.0 36-37 0.04755640192376759 3.419460210228414E-4 0.0 2.597901588290418E-4 0.0 38-39 0.05252646151504114 3.6118973649165926E-4 0.0 2.8421487461638764E-4 0.0 40-41 0.05823814429476683 3.796933090578304E-4 0.0 2.9457687525344346E-4 0.0 42-43 0.06472179612195317 4.3224345514575617E-4 0.0 3.308438774831387E-4 0.0 44-45 0.07217725558031482 4.544477422251615E-4 0.0 3.382453065096072E-4 0.0 46-47 0.08126176956740216 5.040373167024999E-4 0.0 3.7229188003136193E-4 0.0 48-49 0.0907962904392988 5.425247476401357E-4 0.0 3.804334519604772E-4 0.0 50-51 0.10102136463936492 5.64729034719541E-4 7.401429026468427E-7 3.9523631001341406E-4 0.0 52-53 0.1127814952195206 5.995157511439426E-4 1.4802858052936855E-6 4.1299973967693824E-4 0.0 54-55 0.12434030693015635 6.157988950021732E-4 1.4802858052936855E-6 4.2336174031399405E-4 0.0 56-57 0.13756147960013693 6.202397524180543E-4 1.4802858052936855E-6 4.3224345514575617E-4 1.4802858052936855E-6 58-59 0.1507056774082422 6.269010385418759E-4 1.4802858052936855E-6 4.396448841722246E-4 2.960571610587371E-6 60-61 0.1643479913898288 6.579870404530433E-4 1.4802858052936855E-6 4.581484567383957E-4 4.440857415881057E-6 62-63 0.18046904395237967 6.846321849483296E-4 1.4802858052936855E-6 4.640695999595704E-4 4.440857415881057E-6 64-65 0.19697867153882015 6.898131852668575E-4 1.4802858052936855E-6 4.7147102898603883E-4 4.440857415881057E-6 66-67 0.21495304192959874 6.935138997800918E-4 1.4802858052936855E-6 4.7813231510986044E-4 4.440857415881057E-6 68-69 0.23630246395644694 6.979547571959728E-4 1.4802858052936855E-6 4.8183302962309465E-4 4.440857415881057E-6 70-71 0.25817442687256376 7.001751859039133E-4 2.960571610587371E-6 4.929351731627974E-4 4.440857415881057E-6 72-73 0.2818441968992098 7.090569007356754E-4 2.960571610587371E-6 4.973760305786784E-4 4.440857415881057E-6 74-75 0.30940119745055705 7.11277329443616E-4 2.960571610587371E-6 5.062577454104405E-4 4.440857415881057E-6 76-77 0.33669840784307525 7.134977581515564E-4 3.700714513234214E-6 5.26981746684552E-4 4.440857415881057E-6 78-79 0.36772889903654415 7.171984726647906E-4 5.1810003185278994E-6 5.358634615163142E-4 4.440857415881057E-6 80-81 0.40522897934204904 7.208991871780249E-4 5.921143221174742E-6 5.662093205248348E-4 4.440857415881057E-6 82-83 0.446025656135943 7.290407591071401E-4 5.921143221174742E-6 5.7435089245395E-4 4.440857415881057E-6 84-85 0.49702002184250527 7.334816165230212E-4 5.921143221174742E-6 5.817523214804185E-4 4.440857415881057E-6 86-87 0.5571188853945261 7.357020452309617E-4 6.661286123821585E-6 5.891537505068869E-4 4.440857415881057E-6 88-89 0.627372509570862 7.357020452309617E-4 7.401429026468428E-6 5.921143221174742E-4 4.440857415881057E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCGAC 13590 0.0 55.67337 1 TTCGACT 16650 0.0 46.60269 2 TTCGTAT 13750 0.0 46.096863 1 CCGATAG 8110 0.0 44.88595 1 CGATAGT 8825 0.0 44.79789 2 CGATTCA 10760 0.0 37.89993 1 CGTCTCA 15575 0.0 36.906994 1 CGTCGTA 5910 0.0 33.011574 1 TCGACTC 27010 0.0 30.066294 3 GTTGTAT 34540 0.0 29.88182 1 CGCACTA 8615 0.0 28.77092 2 GTTTTTT 377325 0.0 26.604456 1 CGCGTGT 60645 0.0 21.530739 1 GATAGTG 20605 0.0 21.126104 3 TGTATTA 52830 0.0 20.504698 3 GTACGAC 5740 0.0 20.227758 1 GCGTGTT 63055 0.0 20.122168 2 GTCTTAT 29680 0.0 20.072906 1 ATATGAC 28620 0.0 20.034903 1 TATGACT 30220 0.0 19.914406 2 >>END_MODULE