FastQCFastQC Report
Fri 27 May 2016
SRR1233060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51912944
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT924550.17809623742394573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT568850.060.3099941
GCGTGTT677150.049.0413442
GTTTTTT6028300.041.6307371
CGTGTTT912700.035.7086683
CGCGTGC29500.025.1851391
CGCGTTT38300.024.3907281
CTGGAAT773700.023.2724461
CAAAACG221900.020.4644479
TCTACGC59450.020.247957
GTGTTTT1728200.018.276984
TATGGCG830950.017.7130997
CTTTTAT860150.017.0592231
CGGCGAG37050.016.9565331
GTCCGTT64900.016.9552159
CTTTATT1443400.016.8347031
CCCGTAT68250.016.8090841
TGGCGAG858950.016.6962919
ATGGCGA885050.016.665188
TACGCAT59700.016.4417369
AGTATCG131350.016.18216328-29
Produced by FastQC (version 0.11.5)