##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233060_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51912944 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.97211656884649 31.0 30.0 34.0 16.0 34.0 2 26.15633625016528 31.0 27.0 34.0 2.0 34.0 3 27.076651692109774 31.0 27.0 34.0 2.0 34.0 4 30.785381830781933 35.0 32.0 37.0 10.0 37.0 5 30.52335681443919 35.0 32.0 37.0 2.0 37.0 6 31.10774700043981 35.0 32.0 37.0 10.0 37.0 7 30.8902890192473 35.0 32.0 37.0 10.0 37.0 8 30.815811004669666 35.0 32.0 37.0 2.0 37.0 9 32.672958886708486 38.0 32.0 39.0 17.0 39.0 10-11 32.808518025870384 38.0 33.0 39.0 13.5 39.0 12-13 32.257765914797666 38.0 32.0 39.0 8.5 39.0 14-15 33.08569585265671 38.0 32.0 41.0 6.0 41.0 16-17 33.014665186393586 38.0 32.0 40.5 10.0 41.0 18-19 33.44465976539493 38.0 32.0 40.0 14.5 41.0 20-21 33.67236109745577 38.5 33.0 41.0 15.5 41.0 22-23 33.53687415608716 38.0 33.0 40.5 15.0 41.0 24-25 33.377657892027855 38.0 33.0 41.0 13.0 41.0 26-27 33.33127620348405 38.0 33.0 41.0 10.0 41.0 28-29 33.29310883428225 38.0 33.0 40.0 9.5 41.0 30-31 33.3269300234639 38.0 33.0 40.0 9.5 41.0 32-33 32.9960706524369 38.0 32.0 40.0 8.0 41.0 34-35 32.90162190570429 38.0 32.0 40.0 8.0 41.0 36-37 32.661026390643535 38.0 31.0 40.0 7.5 41.0 38-39 32.63300592237651 38.0 31.5 40.0 4.0 41.0 40-41 32.42458823949572 38.0 31.0 40.0 2.0 41.0 42-43 32.5381996732838 38.0 31.0 40.0 2.0 41.0 44-45 32.43546680766168 38.0 31.0 40.0 2.0 41.0 46-47 32.342694685163686 38.0 31.0 40.0 2.0 41.0 48-49 32.19507471970767 38.0 31.0 40.0 2.0 41.0 50-51 31.255474964008975 36.5 29.5 39.0 2.0 40.5 52-53 31.354566300458707 37.0 30.0 39.5 2.0 40.0 54-55 31.693283499776086 38.0 30.5 40.0 2.0 41.0 56-57 31.725187440727694 38.0 31.0 40.0 2.0 41.0 58-59 31.57601697565062 37.5 30.0 40.0 2.0 41.0 60-61 31.318187020947995 37.0 30.0 40.0 2.0 41.0 62-63 31.034027910264538 36.5 29.5 40.0 2.0 41.0 64-65 30.750577110402368 36.0 29.0 39.5 2.0 41.0 66-67 30.40234276638212 36.0 28.5 39.0 2.0 41.0 68-69 29.935779099717404 35.0 28.0 39.0 2.0 40.0 70-71 29.595673373869914 35.0 28.0 39.0 2.0 40.0 72-73 29.34598619565864 35.0 28.0 38.0 2.0 40.0 74-75 29.036080673829634 34.5 27.5 37.0 2.0 39.5 76-77 28.56270400499729 34.0 26.0 37.0 2.0 39.0 78-79 28.1135700953504 34.0 26.0 36.0 2.0 39.0 80-81 27.69256169521035 34.0 26.0 36.0 2.0 38.5 82-83 27.37722117050422 34.0 25.5 35.5 2.0 37.0 84-85 27.054161954290244 34.0 25.0 35.0 2.0 37.0 86-87 26.560857866199996 33.5 24.0 35.0 2.0 36.5 88-89 26.09915206311551 33.0 21.5 35.0 2.0 36.0 90-91 25.726150264180742 33.0 20.0 35.0 2.0 36.0 92-93 25.391617425896712 33.0 19.0 35.0 2.0 35.0 94-95 24.911622195805347 32.0 14.0 35.0 2.0 35.0 96-97 24.350001735597964 32.0 2.0 35.0 2.0 35.0 98-99 23.491906835412763 32.0 2.0 35.0 2.0 35.0 100-101 20.130767492207724 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.8392843855523573 1101 2 7.315388916286405 1101 3 6.341818403105595 1101 4 6.2626351142837855 1101 5 6.35537492720135 1101 6 5.759263691309098 1101 7 6.0360208610115365 1101 8 6.079964951890812 1101 9 5.869817608997362 1101 10-11 5.71297626830458 1101 12-13 6.1012475045051495 1101 14-15 6.577782383011527 1101 16-17 6.199385514627842 1101 18-19 5.476375023568423 1101 20-21 5.235629353687187 1101 22-23 5.07330169648867 1101 24-25 4.851044542954334 1101 26-27 4.552973256383638 1101 28-29 4.151388414456086 1101 30-31 3.7994167101331655 1101 32-33 3.9720635246051827 1101 34-35 3.696009452660295 1101 36-37 3.744991615079382 1101 38-39 3.4934037141592356 1101 40-41 3.8780280419709285 1101 42-43 3.7566642790020843 1101 44-45 3.6813455774217374 1101 46-47 3.553533841625427 1101 48-49 3.6156078995278023 1101 50-51 3.3939797322794156 1101 52-53 3.40161969999388 1101 54-55 3.579631595303141 1101 56-57 3.4781851075009698 1101 58-59 3.367337796226213 1101 60-61 3.3684463498305917 1101 62-63 3.3308315557936687 1101 64-65 3.35629123290094 1101 66-67 3.2879368667932205 1101 68-69 3.4362528632180585 1101 70-71 3.362122860581316 1101 72-73 3.1890646586097304 1101 74-75 3.0003411955382084 1101 76-77 3.0776044970417793 1101 78-79 3.091107053522464 1101 80-81 2.912420007151237 1101 82-83 2.8432092455292555 1101 84-85 2.8348906052663985 1101 86-87 2.7824596644166455 1101 88-89 2.740308404679041 1101 90-91 2.78981822798038 1101 92-93 2.6722318658426865 1101 94-95 2.646464875927922 1101 96-97 2.705184648664744 1101 98-99 2.8123102665774766 1101 100-101 2.1846108152075736 1102 1 3.680442153482467 1102 2 7.166679354293084 1102 3 6.208773566526933 1102 4 6.112673095029162 1102 5 6.286562573285035 1102 6 5.655775151091721 1102 7 5.951429656635931 1102 8 6.04669447931192 1102 9 5.767490401895206 1102 10-11 5.549649150963575 1102 12-13 5.926249619489514 1102 14-15 6.448335862182464 1102 16-17 5.994640979013688 1102 18-19 5.173327812602864 1102 20-21 4.985484440861562 1102 22-23 4.87282705059549 1102 24-25 4.555529546260168 1102 26-27 4.306312255383588 1102 28-29 4.01609890197129 1102 30-31 3.6265935904890014 1102 32-33 3.8424513840723975 1102 34-35 3.560014478502719 1102 36-37 3.431097272887637 1102 38-39 3.338052215226419 1102 40-41 3.648929519355981 1102 42-43 3.4522108458484873 1102 44-45 3.5426313213686313 1102 46-47 3.392408279737257 1102 48-49 3.404962265754257 1102 50-51 3.2129467111775476 1102 52-53 3.2396240414696535 1102 54-55 3.3349220357465157 1102 56-57 3.3130108036664865 1102 58-59 3.2254206095542806 1102 60-61 3.220174490204009 1102 62-63 3.101729944156407 1102 64-65 3.1319688947421973 1102 66-67 3.1146521641003524 1102 68-69 3.3562729269969367 1102 70-71 3.2864195621798373 1102 72-73 3.118383476489093 1102 74-75 2.8794870883594044 1102 76-77 2.9431317032318525 1102 78-79 2.973721915934803 1102 80-81 2.815486677973709 1102 82-83 2.8136627415788205 1102 84-85 2.7666190303315155 1102 86-87 2.6936848304520176 1102 88-89 2.6282587766228254 1102 90-91 2.703135577892315 1102 92-93 2.608626373336133 1102 94-95 2.573226902689374 1102 96-97 2.508231800836498 1102 98-99 2.630662815175409 1102 100-101 2.1521844455824386 1103 1 3.4568435808050246 1103 2 6.951430759921713 1103 3 6.007736169065822 1103 4 5.981736921096218 1103 5 6.179184084097191 1103 6 5.5346411019907045 1103 7 5.852917862898714 1103 8 5.925935497968336 1103 9 5.666400551846333 1103 10-11 5.377438154055547 1103 12-13 5.820390960990178 1103 14-15 6.2318673536919 1103 16-17 5.815867495982566 1103 18-19 5.1070858684116445 1103 20-21 4.831757273509297 1103 22-23 4.72337586430497 1103 24-25 4.3340972036212975 1103 26-27 4.162865606100674 1103 28-29 3.84787353853298 1103 30-31 3.4347156624591477 1103 32-33 3.5072935565539396 1103 34-35 3.3207766400046914 1103 36-37 3.3006183530497175 1103 38-39 3.1306035879388148 1103 40-41 3.459728085904935 1103 42-43 3.1577339733739542 1103 44-45 3.225906383443643 1103 46-47 3.120293218419608 1103 48-49 3.221287129921258 1103 50-51 3.034546578359791 1103 52-53 2.9829556165718785 1103 54-55 3.1388874305924688 1103 56-57 3.0512346894447404 1103 58-59 2.945824089898263 1103 60-61 2.918413109034965 1103 62-63 2.847862246628484 1103 64-65 2.8461870798635083 1103 66-67 2.875821045122091 1103 68-69 3.089329940972732 1103 70-71 3.0637695481888976 1103 72-73 2.8817485595939054 1103 74-75 2.4224652370772546 1103 76-77 2.6320164816348175 1103 78-79 2.6497922214235885 1103 80-81 2.537725864113387 1103 82-83 2.5549965620335016 1103 84-85 2.5275232249926383 1103 86-87 2.445496559502139 1103 88-89 2.385102390908962 1103 90-91 2.438567842568883 1103 92-93 2.335727414842772 1103 94-95 2.2724033089487072 1103 96-97 2.247225009565561 1103 98-99 2.3325436757448728 1103 100-101 1.7748195740502304 1104 1 3.3627440948351612 1104 2 6.866961438473421 1104 3 5.852978161646664 1104 4 5.683595807666581 1104 5 5.958049612639488 1104 6 5.277323625506181 1104 7 5.7254926591047735 1104 8 5.536489394013774 1104 9 5.4839370277764665 1104 10-11 5.329095118180312 1104 12-13 5.747838153771486 1104 14-15 6.173531377237598 1104 16-17 5.635069325706873 1104 18-19 4.796031010376268 1104 20-21 4.5270507975497765 1104 22-23 4.447497370286996 1104 24-25 4.296806161979397 1104 26-27 4.044072080274688 1104 28-29 3.708000846436086 1104 30-31 3.368237156962792 1104 32-33 3.4639299072330587 1104 34-35 3.1714015154301087 1104 36-37 3.3160030102183775 1104 38-39 3.0801059296270026 1104 40-41 3.4256883991105127 1104 42-43 3.1590074784087285 1104 44-45 3.059824169632215 1104 46-47 3.0148386642754197 1104 48-49 3.1650545363412164 1104 50-51 2.9101112549198227 1104 52-53 2.8527067587496973 1104 54-55 2.9549391864336982 1104 56-57 2.9007686408496127 1104 58-59 2.8767364850507313 1104 60-61 2.8114537608408057 1104 62-63 2.660318157142246 1104 64-65 2.764573049745703 1104 66-67 2.715908001352144 1104 68-69 2.9672854725898006 1104 70-71 2.9278355948832093 1104 72-73 2.7865811577635036 1104 74-75 2.515923864342966 1104 76-77 2.6792161607426586 1104 78-79 2.6575665797015233 1104 80-81 2.5441138882767724 1104 82-83 2.5278148558709113 1104 84-85 2.4485170639224236 1104 86-87 2.332932643240067 1104 88-89 2.365878272577607 1104 90-91 2.436963819752261 1104 92-93 2.2790845326776115 1104 94-95 2.302339886480919 1104 96-97 2.321044075934907 1104 98-99 2.383212177777576 1104 100-101 1.8549991373485746 1105 1 3.21188684356375 1105 2 6.734816582840793 1105 3 5.59055480709646 1105 4 5.527844842468308 1105 5 5.756478622798721 1105 6 5.212841013087637 1105 7 5.532435780064937 1105 8 5.501933306044446 1105 9 5.165824884395494 1105 10-11 4.876219051337763 1105 12-13 5.403373746870532 1105 14-15 5.780761574145107 1105 16-17 5.1819301518323115 1105 18-19 4.591808005240544 1105 20-21 4.500531946808039 1105 22-23 4.1383225240933825 1105 24-25 3.776932831656545 1105 26-27 3.540370004296342 1105 28-29 3.032405929883005 1105 30-31 3.0143784085187306 1105 32-33 3.034878752924371 1105 34-35 2.9297809449695684 1105 36-37 3.08074459914641 1105 38-39 3.042059167721213 1105 40-41 3.031945332065831 1105 42-43 2.9835075725049087 1105 44-45 2.8875219989052 1105 46-47 2.650366153754323 1105 48-49 2.7690257924926343 1105 50-51 2.545597817738223 1105 52-53 2.553143937911777 1105 54-55 2.8426125191479557 1105 56-57 2.87978146239017 1105 58-59 2.534422245029962 1105 60-61 2.5948633053007413 1105 62-63 2.4151764329720926 1105 64-65 2.3930501425077075 1105 66-67 2.361383372865699 1105 68-69 2.54834156037483 1105 70-71 2.4176459731934727 1105 72-73 2.3786483671170586 1105 74-75 2.1401990930683112 1105 76-77 2.2858064284254276 1105 78-79 2.3118862313907336 1105 80-81 2.0994495652689515 1105 82-83 2.128449559182581 1105 84-85 2.022771978149347 1105 86-87 1.7921206497410616 1105 88-89 1.8848556334092201 1105 90-91 1.8068456601549165 1105 92-93 1.8265705644480512 1105 94-95 1.7968024802251499 1105 96-97 1.7815315386824295 1105 98-99 1.7501784439998538 1105 100-101 1.1101665203386908 1106 1 3.2674717339454347 1106 2 6.633875865286644 1106 3 5.649330535265612 1106 4 5.575798047082625 1106 5 5.793432928475998 1106 6 5.222971167617647 1106 7 5.451727471776984 1106 8 5.601722022447735 1106 9 5.212478456082895 1106 10-11 5.0010162995553955 1106 12-13 5.491038563916458 1106 14-15 5.8458469043024515 1106 16-17 5.326096429078909 1106 18-19 4.7106396907280015 1106 20-21 4.518853944317804 1106 22-23 4.4539232538591165 1106 24-25 4.274337737998579 1106 26-27 3.9339052678161366 1106 28-29 3.511709249360912 1106 30-31 3.192952952387401 1106 32-33 3.3219670803145434 1106 34-35 3.011175764184838 1106 36-37 3.176305617592977 1106 38-39 3.0572827660143176 1106 40-41 3.3021091944465653 1106 42-43 3.118151624662456 1106 44-45 2.9092724601814766 1106 46-47 2.7736201670001783 1106 48-49 2.9033513488062184 1106 50-51 2.72951853496917 1106 52-53 2.75491306189965 1106 54-55 2.973105254214893 1106 56-57 2.889259301871718 1106 58-59 2.7128160585673022 1106 60-61 2.7607817603947 1106 62-63 2.6588401862833457 1106 64-65 2.604911296009245 1106 66-67 2.565468142984141 1106 68-69 2.687728072413858 1106 70-71 2.767426912590114 1106 72-73 2.5651070156372953 1106 74-75 2.3725657942984526 1106 76-77 2.488870443978808 1106 78-79 2.4711740862769283 1106 80-81 2.28031306869865 1106 82-83 2.3731522139629426 1106 84-85 2.270392456425906 1106 86-87 2.1760024430792804 1106 88-89 2.041362472092093 1106 90-91 2.1585614296006703 1106 92-93 2.1532179766363697 1106 94-95 2.2264579009754932 1106 96-97 2.181131910893207 1106 98-99 2.118154275272534 1106 100-101 1.554999362080597 1107 1 2.81029306706818 1107 2 6.018744503430593 1107 3 5.231184283724705 1107 4 4.573988859914973 1107 5 4.272648664644922 1107 6 4.400448161685066 1107 7 1.271395269196006 1107 8 3.67490160435867 1107 9 3.948352201935162 1107 10-11 4.644638700107926 1107 12-13 4.557059754972084 1107 14-15 4.68724262219482 1107 16-17 4.5884080041604065 1107 18-19 4.139884901867646 1107 20-21 4.242102664933526 1107 22-23 3.643080372011987 1107 24-25 3.4315804789816298 1107 26-27 3.215444635398633 1107 28-29 3.054346687411929 1107 30-31 2.8606600459524785 1107 32-33 3.110084536049442 1107 34-35 2.5452907960302475 1107 36-37 2.4569547924430815 1107 38-39 2.5146206452465023 1107 40-41 3.0040517226047285 1107 42-43 2.8089455625284394 1107 44-45 2.8550119233234525 1107 46-47 2.678072531517632 1107 48-49 2.8681516980664057 1107 50-51 2.573757462157179 1107 52-53 2.357378312201625 1107 54-55 2.696424534950424 1107 56-57 2.6885082907196107 1107 58-59 2.65679329212659 1107 60-61 2.648798521195822 1107 62-63 2.425929675094398 1107 64-65 2.397432613506819 1107 66-67 2.5664476165454495 1107 68-69 2.7650718672195786 1107 70-71 2.7616834054507926 1107 72-73 2.595510086258301 1107 74-75 2.3659926792092456 1107 76-77 2.491685645735263 1107 78-79 2.4701318069099933 1107 80-81 2.265801193450077 1107 82-83 2.25158504927413 1107 84-85 2.304485013650268 1107 86-87 2.140155521339249 1107 88-89 2.013782370096198 1107 90-91 2.0122573391473466 1107 92-93 2.080500894575408 1107 94-95 1.8782733995381058 1107 96-97 1.8770883490263728 1107 98-99 2.0429747837259633 1107 100-101 1.3963181220436347 1108 1 2.895358677391581 1108 2 6.354601989987415 1108 3 5.219561050495869 1108 4 5.196383203717179 1108 5 5.420372783463055 1108 6 4.795164176110948 1108 7 4.942928354999978 1108 8 4.87938925232832 1108 9 4.9267684655558845 1108 10-11 4.756917849597251 1108 12-13 5.1435679658356825 1108 14-15 5.4501453363492836 1108 16-17 5.0042303888382875 1108 18-19 4.43408684049291 1108 20-21 4.0510307187069685 1108 22-23 3.94187755237067 1108 24-25 3.6832502230509974 1108 26-27 3.525767884474142 1108 28-29 3.129597018611584 1108 30-31 2.756160913991259 1108 32-33 3.0131125615149443 1108 34-35 2.805739612280224 1108 36-37 2.823703600487466 1108 38-39 2.6434515514218404 1108 40-41 2.8368801676584496 1108 42-43 2.505518225813322 1108 44-45 2.6995864709226822 1108 46-47 2.484287957624428 1108 48-49 2.619985929402752 1108 50-51 2.3458253066932393 1108 52-53 2.384770200824562 1108 54-55 2.546313310657901 1108 56-57 2.4455668122438112 1108 58-59 2.3888988022910134 1108 60-61 2.3809562830041173 1108 62-63 2.307981538400302 1108 64-65 2.151949959198543 1108 66-67 2.2600226917175057 1108 68-69 2.3869059928779706 1108 70-71 2.3909441336580315 1108 72-73 2.2230408759083 1108 74-75 1.9972659092364182 1108 76-77 2.1826893390463233 1108 78-79 2.1061664931544044 1108 80-81 1.943974515142802 1108 82-83 1.9072242552855805 1108 84-85 1.815671927060034 1108 86-87 1.7531094584956186 1108 88-89 1.7532191916935993 1108 90-91 1.7802647813814012 1108 92-93 1.8810810143367576 1108 94-95 1.8819428833738172 1108 96-97 1.8866084137104444 1108 98-99 1.9801883123899877 1108 100-101 1.3700688230622475 1109 1 2.9278664882388306 1109 2 5.572158476112268 1109 3 5.0836747714130155 1109 4 5.107842534248061 1109 5 5.136416571667983 1109 6 4.78393692114582 1109 7 4.823208685757045 1109 8 4.866825399004409 1109 9 4.793289341598683 1109 10-11 4.724375010157779 1109 12-13 5.087518072569342 1109 14-15 5.256472315528544 1109 16-17 5.023856505434345 1109 18-19 4.488689138707272 1109 20-21 3.694331691647008 1109 22-23 3.8597295839173142 1109 24-25 3.464048071936652 1109 26-27 3.25311733776163 1109 28-29 3.1407045022490365 1109 30-31 2.7691190027755184 1109 32-33 2.813990219661342 1109 34-35 2.670424552845841 1109 36-37 2.9023781419812913 1109 38-39 2.694180396592806 1109 40-41 2.5390364630534066 1109 42-43 2.469200173554132 1109 44-45 2.6289441091275734 1109 46-47 2.2150529451525536 1109 48-49 2.42328667474564 1109 50-51 2.143176852904059 1109 52-53 2.2082002006015884 1109 54-55 2.425668134410948 1109 56-57 2.411249762528506 1109 58-59 2.1539024074533906 1109 60-61 1.9366427777302349 1109 62-63 2.05517517356159 1109 64-65 2.0508778790351023 1109 66-67 2.0827381670081984 1109 68-69 2.2505654392634895 1109 70-71 2.10106204336293 1109 72-73 1.964021752572382 1109 74-75 1.7429744682620907 1109 76-77 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-2.8048995289653185 2314 72-73 -3.9353687704796094 2314 74-75 -4.772010299928681 2314 76-77 -2.669063231682866 2314 78-79 -1.9290585311606208 2314 80-81 -4.669441565917605 2314 82-83 -3.136036348145417 2314 84-85 -2.3005437048505506 2314 86-87 -1.9996510824878122 2314 88-89 -3.885126497826608 2314 90-91 -3.965978812638024 2314 92-93 -2.8144613301956447 2314 94-95 -4.140677693538461 2314 96-97 -3.5855380972128543 2314 98-99 -3.385153101870756 2314 100-101 -3.7066796521379217 2315 1 -25.97373312397639 2315 2 -22.69620621784622 2315 3 -23.685417019006938 2315 4 -27.277254191614542 2315 5 -27.127122845773517 2315 6 -27.747592625410388 2315 7 -27.482722658710415 2315 8 -27.412930472850345 2315 9 -29.2483534557397 2315 10-11 -29.426508522209026 2315 12-13 -28.92215451369074 2315 14-15 -26.830230229176635 2315 16-17 -29.60440992033572 2315 18-19 -30.108631620998366 2315 20-21 -30.31712558592757 2315 22-23 -30.18605545992881 2315 24-25 -26.95505407409957 2315 26-27 -27.1355996855423 2315 28-29 -27.021461342205058 2315 30-31 -27.847039693281296 2315 32-33 -22.655052897796942 2315 34-35 -20.58467107857946 2315 36-37 -26.77190877191985 2315 38-39 -25.39276565318687 2315 40-41 -26.516365176772457 2315 42-43 -23.84469179416707 2315 44-45 -16.650889786835624 2315 46-47 -18.298663771733008 2315 48-49 -19.377166983961857 2315 50-51 -14.601518823695379 2315 52-53 -14.176361996185701 2315 54-55 -14.168188624696537 2315 56-57 -22.034159304180157 2315 58-59 -23.00824856035907 2315 60-61 -18.22138552184169 2315 62-63 -16.809095060705783 2315 64-65 -14.70374506383816 2315 66-67 -13.765636289595857 2315 68-69 -13.391287066227356 2315 70-71 -14.717624843785293 2315 72-73 -16.199142387256494 2315 74-75 -14.709826651892676 2315 76-77 -12.71593539820924 2315 78-79 -12.023726042883965 2315 80-81 -11.499576122776638 2315 82-83 -13.142593743963207 2315 84-85 -11.791104034671338 2315 86-87 -10.648809209436813 2315 88-89 -10.372585347648632 2315 90-91 -10.13733026753377 2315 92-93 -11.273930671201583 2315 94-95 -10.661913740249776 2315 96-97 -10.04253008958529 2315 98-99 -9.637153415775762 2315 100-101 -8.010425894896759 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2215398.0 3 60288.0 4 67650.0 5 105282.0 6 175100.0 7 279163.0 8 404988.0 9 568065.0 10 610291.0 11 535044.0 12 433524.0 13 361565.0 14 329699.0 15 338223.0 16 378494.0 17 407101.0 18 428766.0 19 454061.0 20 493192.0 21 539390.0 22 591224.0 23 662916.0 24 747725.0 25 844878.0 26 959464.0 27 1084812.0 28 1221744.0 29 1376574.0 30 1540302.0 31 1732685.0 32 2012409.0 33 2399050.0 34 2960208.0 35 3823899.0 36 5201405.0 37 7316820.0 38 6966314.0 39 1284745.0 40 486.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.323693697437577 14.399605629357847 11.396265604732768 44.880435068471805 2 16.612806775651997 18.34318066431763 51.21279347257346 13.831219087456917 3 20.297803864432396 21.49100142826476 36.00533943438939 22.20585527291345 4 21.29346261577082 29.00440779726678 31.888349058562554 17.81378052839985 5 20.669292068642804 31.032332472299167 33.27320169915966 15.025173759898367 6 16.583599441033062 33.3034048426357 34.34017324795364 15.772822468377596 7 13.899705080388019 16.942858957668964 51.87411576836901 17.283320193574006 8 15.326614803596367 21.130573264645612 43.02075842814549 20.52205350361253 9 17.524119357261103 20.539520008539753 41.665727802444415 20.270632831754728 10-11 19.897019328653244 31.313903779498798 31.720214017788688 17.06886287405927 12-13 18.12810204986805 25.27739627484221 38.474526940370026 18.11997473491971 14-15 18.830359536467185 25.918222415062793 37.28160289434464 17.969815154125385 16-17 19.621841453314115 26.33522879106007 35.46479806255527 18.57813169307054 18-19 20.00548645277711 26.781100174602447 34.29675410450517 18.916659268115275 20-21 19.89903115362726 27.31871830063108 33.780678164333175 19.001572381408486 22-23 19.87994762605273 27.886748912305954 34.22791625742368 18.00538720421764 24-25 19.780127910089675 28.085939384991743 33.42735166197269 18.706581042945892 26-27 20.10322730344537 28.24886473253136 32.652885778105585 18.995022185917687 28-29 20.397743272876916 28.28785747387319 31.527588457851085 19.786810795398807 30-31 19.815038518144394 28.696308823942946 31.547451793215366 19.941200864697297 32-33 20.126708795397217 28.647271530791624 31.316862615450887 19.90915705836027 34-35 19.853448654394278 29.264042199199807 30.627844845957796 20.254664300448123 36-37 19.959128850601402 28.763765306307796 31.08294554653515 20.194160296555648 38-39 20.193646093766 28.273733627744253 31.389995965101292 20.142624313388456 40-41 20.44263605103239 28.556102029478286 31.0294578881886 19.971804031300724 42-43 20.388792414970663 28.99829798149333 31.054698341624903 19.558211261911104 44-45 20.373844994019315 28.66660984127539 31.116155786202327 19.84338937850297 46-47 20.02532047273454 29.006042626838084 30.726693184248884 20.241943716178493 48-49 20.465624513800694 28.882133434042938 30.371477749505864 20.280764302650503 50-51 20.10443397824816 28.850448711143343 30.83303028122239 20.2120870293861 52-53 20.330252745051993 29.095434878228893 30.650748346959777 19.923564029759337 54-55 20.126560776633514 29.231685694019717 30.435437382590518 20.20631614675625 56-57 20.764338679235454 28.74886393181553 30.133714440671678 20.353082948277333 58-59 20.352254826024968 29.128099415096447 29.877243937355292 20.64240182152329 60-61 20.629792853813537 29.098514430860494 29.789043028392225 20.482649686933737 62-63 19.983928498547694 30.083021296344057 29.749018549601765 20.18403165550648 64-65 20.010642305813313 29.53049123872164 30.00685664799941 20.452009807465636 66-67 20.27229665834024 29.023618872520927 30.132620177253273 20.571464291885558 68-69 20.87724495942543 29.348805097215546 29.30433188692986 20.469618056429166 70-71 20.214425132127563 29.707603292502082 29.42927342662213 20.648698148748224 72-73 20.033604310453175 29.76804099260093 29.568353769715884 20.630000927230014 74-75 20.22032813709619 29.196622870125815 29.989249347194423 20.593799645583573 76-77 20.469673998222316 29.26543335808136 29.399679123542427 20.8652135201539 78-79 20.360757269815657 29.884335216933835 29.005922996993718 20.748984516256794 80-81 20.305075464873166 29.606079329780215 29.81630027699162 20.272544928354996 82-83 20.26411400497489 29.682270302244028 29.40093154623539 20.65268414654569 84-85 20.15433303776112 29.773052012695732 29.254738286063354 20.817876663479787 86-87 20.5139940756411 30.01952607257683 28.845650917243702 20.620828934538363 88-89 20.337295167063985 29.710318143174625 29.348056399342326 20.604330290419067 90-91 20.30025955568432 30.00772786994872 29.020733393013597 20.67127918135337 92-93 20.246378145770212 30.056815613045888 29.027756195128823 20.669050046055077 94-95 20.069394656748006 29.99124310154301 28.93797754431849 21.001384697390495 96-97 20.05887972396686 29.861510038770444 29.16401762327196 20.915592613990736 98-99 20.486416755490424 29.76616988933327 28.917830911284568 20.829582443891734 100-101 20.955233837613353 30.25441639589794 27.907242799121263 20.88310696736744 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3243.0 1 18373.5 2 25783.5 3 19203.0 4 22810.5 5 31152.0 6 35445.0 7 36624.0 8 42821.5 9 50516.5 10 59420.0 11 69579.5 12 81967.0 13 95728.0 14 112455.5 15 131770.0 16 151448.0 17 173192.0 18 198742.5 19 225545.5 20 256941.5 21 298568.0 22 344686.0 23 395515.0 24 461058.0 25 538404.5 26 623649.0 27 737629.5 28 870766.0 29 1022936.5 30 1203237.5 31 1360527.0 32 1569125.0 33 1803978.0 34 2007808.0 35 2201220.0 36 2343903.5 37 2369089.0 38 2271806.5 39 2130386.5 40 2038922.0 41 1947760.0 42 1801541.5 43 1716634.0 44 1660687.0 45 1601965.0 46 1540339.0 47 1463004.0 48 1388810.0 49 1321461.0 50 1225010.5 51 1108281.5 52 1008057.5 53 918813.0 54 826144.0 55 727973.5 56 636329.0 57 538830.0 58 450028.5 59 374107.5 60 294313.5 61 224636.5 62 171815.5 63 127716.5 64 92005.5 65 66271.5 66 48296.0 67 35941.0 68 26875.0 69 20226.0 70 16188.5 71 14053.0 72 11973.0 73 12155.0 74 11584.0 75 8417.5 76 7338.5 77 6807.0 78 6183.5 79 5503.5 80 4484.5 81 3302.0 82 2423.5 83 1711.0 84 941.0 85 468.0 86 304.5 87 209.0 88 157.5 89 122.5 90 109.5 91 85.5 92 65.5 93 51.5 94 31.0 95 18.5 96 11.0 97 5.0 98 4.5 99 3.5 100 1.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 4.048959350099659 2 16.71034491898591 3 9.754382259653777 4 7.966783775545459 5 11.182588681543471 6 6.947569762177233 7 8.929015853926527 8 9.327785378536806 9 5.585932479575806 10-11 5.34003426968041 12-13 7.9312223171161325 14-15 8.277952797283081 16-17 6.595905059824771 18-19 4.588164947840369 20-21 5.14958369534966 22-23 4.616810019481846 24-25 2.638619377856898 26-27 3.134511115378084 28-29 2.8478475426090264 30-31 1.2880275100560663 32-33 1.7155605738715183 34-35 1.8637413435847523 36-37 1.903704401738418 38-39 1.8420733372393596 40-41 2.778899420537583 42-43 1.65328708770591 44-45 1.7618245653723663 46-47 1.1191120272431476 48-49 1.2419744486076536 50-51 0.4676820100975202 52-53 0.7841762932959456 54-55 0.8184374979773831 56-57 1.3391217034425942 58-59 0.08189094419303208 60-61 0.5201843301354668 62-63 1.2619819827594443 64-65 0.47304098184067545 66-67 0.0036869417384612206 68-69 0.4693164772161641 70-71 0.03233971858733344 72-73 4.979490278956246E-4 74-75 5.441802722650443E-4 76-77 0.002050548317968636 78-79 0.003320944387203315 80-81 3.5058693646809936E-4 82-83 8.427570588175465E-4 84-85 1.5410414789806563E-4 86-87 0.46721102929550673 88-89 0.31544926444549165 90-91 3.688868040309947E-4 92-93 1.945564867213079E-4 94-95 3.0339254117431677E-4 96-97 6.482005720962387E-4 98-99 0.005844399809034139 100-101 4.854280658789068E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.1912944E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.927474808233605 #Duplication Level Percentage of deduplicated Percentage of total 1 76.12963282145714 30.39663996638804 2 10.142116563483528 8.098982071813147 3 4.265871469219545 5.109764268672776 4 2.3219837961376846 3.7084379810165617 5 1.4784244422739763 2.95148773373855 6 1.0106463507298324 2.421153402527916 7 0.7302841253851955 2.0410880713419215 8 0.5263675449735061 1.6813221513441148 9 0.44637995365161304 1.6040541918892735 >10 2.61160728974892 19.459979975946737 >50 0.20592454107163932 5.656048836749175 >100 0.11680825334975263 8.646285273777622 >500 0.008474373036549812 2.3118106679845725 >1k 0.004940508840351044 3.612684047949849 >5k 3.7358827255881493E-4 1.0118926731676574 >10k+ 1.6437836807894855E-4 1.288368685692362 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 92455 0.17809623742394573 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.008412160173385658 0.0 0.0 5.778905546177462E-6 0.0 2 0.008566264321283724 3.852603697451642E-6 0.0 3.0820829579613134E-5 0.0 3 0.008651021602627661 5.778905546177462E-6 0.0 3.659973512579059E-5 0.0 4 0.009020871557583018 5.778905546177462E-6 0.0 5.201014991559716E-5 0.0 5 0.009142228574052745 5.778905546177462E-6 1.926301848725821E-6 7.705207394903283E-5 0.0 6 0.009344490268168956 7.705207394903283E-6 1.926301848725821E-6 9.631509243629103E-5 0.0 7 0.009456215775395054 7.705207394903283E-6 1.926301848725821E-6 1.0979920537737178E-4 0.0 8 0.009652698563965088 7.705207394903283E-6 1.926301848725821E-6 1.117255072260976E-4 0.0 9 0.009897338898753267 9.631509243629104E-6 1.926301848725821E-6 1.2328331831845253E-4 0.0 10-11 0.010612960035554909 1.5410414789806567E-5 1.926301848725821E-6 1.2713592201590417E-4 0.0 12-13 0.011882392953865225 2.4078773109072758E-5 1.926301848725821E-6 1.309885257133558E-4 0.0 14-15 0.013477370884610203 2.5041924033435667E-5 1.926301848725821E-6 1.3676743125953328E-4 1.926301848725821E-6 16-17 0.015341067923252436 2.696822588216149E-5 1.926301848725821E-6 1.4736209142752528E-4 1.926301848725821E-6 18-19 0.017325158827440033 2.79313768065244E-5 1.926301848725821E-6 2.0033539226748537E-4 1.926301848725821E-6 20-21 0.01945372237028206 2.889452773088731E-5 1.926301848725821E-6 2.1767210890601775E-4 1.926301848725821E-6 22-23 0.02137617161531043 3.563658420142768E-5 1.926301848725821E-6 2.427140329394534E-4 1.926301848725821E-6 24-25 0.02321193727714614 4.237864067196805E-5 1.926301848725821E-6 2.7449801344342945E-4 1.926301848725821E-6 26-27 0.024732752586715173 4.334179159633097E-5 1.926301848725821E-6 3.149503522666717E-4 1.926301848725821E-6 28-29 0.026325804215611426 4.9120697142508425E-5 1.926301848725821E-6 3.621447475604543E-4 1.926301848725821E-6 30-31 0.027683847018963134 5.971535731050044E-5 1.926301848725821E-6 3.7081310587972047E-4 1.926301848725821E-6 32-33 0.029070784350045724 6.4531111932315E-5 1.926301848725821E-6 4.1511804840041434E-4 2.8894527730887315E-6 34-35 0.030537663207850438 7.31994702515812E-5 1.926301848725821E-6 5.393645176432298E-4 3.852603697451642E-6 36-37 0.032018026178596226 8.37941304195732E-5 1.926301848725821E-6 6.44347968398787E-4 3.852603697451642E-6 38-39 0.033545583544635806 1.0016769613374268E-4 1.926301848725821E-6 6.896160618438437E-4 3.852603697451642E-6 40-41 0.03531778124546356 1.0979920537737179E-4 1.926301848725821E-6 7.031001747849245E-4 3.852603697451642E-6 42-43 0.037133320737887644 1.2424646924281545E-4 1.926301848725821E-6 7.165842877260053E-4 3.852603697451642E-6 44-45 0.03915882713182285 1.4254633680571072E-4 1.926301848725821E-6 7.30068400667086E-4 3.852603697451642E-6 46-47 0.041431863313319314 1.868512793264046E-4 1.926301848725821E-6 7.618523811710621E-4 3.852603697451642E-6 48-49 0.04391775584909999 2.234510144521952E-4 1.926301848725821E-6 7.676312867172395E-4 3.852603697451642E-6 50-51 0.046619394191937946 2.4078773109072757E-4 2.8894527730887315E-6 7.791890978095944E-4 4.815754621814552E-6 52-53 0.04959167794452189 2.5716129680489707E-4 3.852603697451642E-6 7.917100598263122E-4 5.778905546177462E-6 54-55 0.052675687204331925 2.5812444772925995E-4 3.852603697451642E-6 8.080836255404817E-4 6.742056470540372E-6 56-57 0.055932100479602925 2.6582965512416323E-4 3.852603697451642E-6 8.148256820110222E-4 9.631509243629104E-6 58-59 0.059549695351510015 2.6871910789725195E-4 3.852603697451642E-6 8.273466440277401E-4 9.631509243629104E-6 60-61 0.06361515540324586 2.7064540974597784E-4 3.852603697451642E-6 8.417939078931836E-4 9.631509243629104E-6 62-63 0.06823539038741475 2.725717115947036E-4 3.852603697451642E-6 8.629832282291677E-4 9.631509243629104E-6 64-65 0.07321006491174917 2.7449801344342945E-4 3.852603697451642E-6 8.938040578087808E-4 9.631509243629104E-6 66-67 0.07886183453591072 2.7546116436779234E-4 3.852603697451642E-6 9.149933781447649E-4 9.631509243629104E-6 68-69 0.08526100927737791 2.7738746621651817E-4 3.852603697451642E-6 9.284774910858456E-4 1.1557811092354924E-5 70-71 0.0924961990211921 2.7738746621651817E-4 4.815754621814552E-6 9.390721512538376E-4 1.3484112941080745E-5 72-73 0.10054525129609293 2.79313768065244E-4 5.778905546177462E-6 9.496668114218296E-4 1.733671663853239E-5 74-75 0.11006021927787413 2.822032208383327E-4 5.778905546177462E-6 9.718192826821766E-4 1.9263018487258208E-5 76-77 0.1208744778566209 2.860558245357844E-4 5.778905546177462E-6 9.997506594887008E-4 1.9263018487258208E-5 78-79 0.13377106873384026 2.870189754601473E-4 5.778905546177462E-6 0.0010170873761272334 2.1189320335984026E-5 80-81 0.14935870329372958 2.927978810063248E-4 5.778905546177462E-6 0.0010353872436901286 2.1189320335984026E-5 82-83 0.16787239036183346 2.9953993747686516E-4 5.778905546177462E-6 0.0010459819038581206 2.311562218470985E-5 84-85 0.19178164890821836 3.0050308840122805E-4 5.778905546177462E-6 0.0010604291677235643 2.311562218470985E-5 86-87 0.2214669235480076 3.0050308840122805E-4 6.742056470540372E-6 0.001117255072260976 2.4078773109072758E-5 88-89 0.25761686719212074 3.0050308840122805E-4 7.705207394903283E-6 0.0011422969962944117 2.696822588216149E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 56885 0.0 60.309994 1 GCGTGTT 67715 0.0 49.041344 2 GTTTTTT 602830 0.0 41.630737 1 CGTGTTT 91270 0.0 35.708668 3 CGCGTGC 2950 0.0 25.185139 1 CGCGTTT 3830 0.0 24.390728 1 CTGGAAT 77370 0.0 23.272446 1 CAAAACG 22190 0.0 20.464447 9 TCTACGC 5945 0.0 20.24795 7 GTGTTTT 172820 0.0 18.27698 4 TATGGCG 83095 0.0 17.713099 7 CTTTTAT 86015 0.0 17.059223 1 CGGCGAG 3705 0.0 16.956533 1 GTCCGTT 6490 0.0 16.955215 9 CTTTATT 144340 0.0 16.834703 1 CCCGTAT 6825 0.0 16.809084 1 TGGCGAG 85895 0.0 16.696291 9 ATGGCGA 88505 0.0 16.66518 8 TACGCAT 5970 0.0 16.441736 9 AGTATCG 13135 0.0 16.182163 28-29 >>END_MODULE