FastQCFastQC Report
Fri 27 May 2016
SRR1233060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51912944
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT522530.10065505050147032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT627900.052.7098121
GCGTGTT722150.045.2217142
GCGCGTT27000.039.4565731
CGCGTTT61650.037.18341
CGTGTTT960550.034.161063
GCGGGTT48000.033.489651
CGGGTTT126600.024.906631
GTTTTTT4969300.024.1868631
TTCGACT89300.023.9828322
CGCTTGT69950.023.2527071
CGATAGT33500.022.9638982
CTGGAAT849250.022.2213921
ATTCGAC89450.022.0117781
CGATTCA66400.021.8457491
CCGATAG34400.021.8440821
TTCGTAT88300.020.467181
GTTGTTT776500.020.309921
TCGACTC105950.020.2586653
GTGTTTT1948100.018.9523224
CCGAAAC66950.018.6116921
Produced by FastQC (version 0.11.5)