FastQCFastQC Report
Fri 27 May 2016
SRR1233059_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233059_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79331550
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1648910.20785047058830944No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT1633700.20593320059925715No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT1351920.17041391476657144No Hit
GTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGT1273910.16058050044402258No Hit
ATACGACTCACTATAGGGAATTGTAATACGACTCACTATAGGGAATTGTA1129310.14235319995638557No Hit
CTCCTATAGTGAGTCGTATTACAATTCTCCTATAGTGAGTCGTATTACAA1034870.13044873067524837No Hit
CGACTCACTATAGGGAATTGTAATACGACTCACTATAGGGAATTGTAATA1017780.12829448056920606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT680900.062.7506521
GCGTGTT796300.052.658392
GTTTTTT8016850.038.306521
CGTGTTT1168850.036.133753
CTGGAAT2105250.035.395371
CCGAAAC113600.023.3310832
GAATATG3416050.023.0617664
GGAATAT3651850.022.5622623
TGGAATA3728850.022.2504922
CCCGAAA116750.021.7793481
CGGCGAG79350.021.570881
TATGGCG3056600.020.8064377
ATGGCGA3099650.020.6184548
TGGCGAG3042250.020.6062249
CGAAACC120350.020.5473373
CGCTTCG76650.019.4581684
CGAGCTA110900.018.91234212-13
GTGTTTT2350200.017.8729114
TACGGTA55300.017.1280779
CTAACGA108700.017.108594-95
Produced by FastQC (version 0.11.5)