FastQCFastQC Report
Fri 27 May 2016
SRR1233059_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233059_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79331550
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC2396200.3020488065593071No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT2170580.2736086714554298No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT1724350.21735992804880278No Hit
GTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGT1548390.1951795975245662No Hit
ATACGACTCACTATAGGGAATTGTAATACGACTCACTATAGGGAATTGTA1543850.19460731575268606No Hit
CGACTCACTATAGGGAATTGTAATACGACTCACTATAGGGAATTGTAATA1318760.1662339888732793No Hit
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA1178960.14861174400348914No Hit
CTCCTATAGTGAGTCGTATTACAATTCTCCTATAGTGAGTCGTATTACAA996780.125647362241126No Hit
ATACGACTCACTATAGGAGAATTGTAATACGACTCACTATAGGAGAATTG982230.12381328739952768No Hit
ATACGACTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAAT941680.11870182796125878No Hit
ATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAAT901610.1136508740847746No Hit
GGGAATTGTAATACGACTCACTATAGGGAATTGTAATACGACTCACTATA878060.11068231995971338No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA866410.10921379955389754No Hit
CGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACG848470.10695240418219486No Hit
ATACGACTCACTATAGGGAAATTGTAATACGACTCACTATAGGGAAATTG825760.10408973479025684No Hit
GTCGTATTACAATTCCCTATAGTGAGTCGTATTACAATTCCCTATAGTGA816410.10291113686799264No Hit
CTCACTATAGGGAATTGTAATACGACTCACTATAGGGAATTGTAATACGA800380.10089050321089149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT733450.062.3581471
GCGTGTT840200.053.5783922
CGATTCA128500.038.572611
CGTGTTT1194950.037.8117643
CTGGAAT2369100.034.573151
CGCGTTT53000.031.9513031
ATTCGAC139250.031.5297781
TTCGACT146850.030.4284442
GTTTTTT7058800.030.2808691
GCGCGTT19400.029.9138661
CGTCTCA124400.026.6135791
CGGCGAG82650.026.1868321
TCGACTC189100.025.0363793
TACGGTA41650.024.5124179
CGCTTCG70150.023.9631254
TAGCGCG14900.023.44489164-65
GAATATG3921650.022.6796764
CCGAAAC114200.022.3707332
CGAGCTA95000.022.2207512-13
GGAATAT4224450.021.8015563
Produced by FastQC (version 0.11.5)