FastQCFastQC Report
Fri 27 May 2016
SRR1233058_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233058_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67436362
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTATTTAAAACCCATAAGTGAAATGGGCAGCTTGGGTCTCTGTCAAA864450.12818751996141192No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC864230.12815489661200882Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT510000.047.863321
GCGTGTT614400.038.1869352
GTTTTTT7072200.037.8252681
CGTGTTT906400.025.9406223
CGCCGTA266800.023.52958746-47
GTCGCCG283150.022.22689844-45
CCGTATC291700.021.58219348-49
CTGGAAT1204100.021.360871
TGGTCGC333500.018.3394442-43
CGCTTCG65750.017.7862973
CGTATCA280750.017.49410250-51
CTTTATT1905750.017.3617061
TCGCCGT281500.017.26355446-47
TCCGATC62450.017.243471
CTCGCGT53050.017.0654141
TCTCGGT372600.016.96329936-37
GATCTCG388450.016.79453334-35
GCCGTAT297150.016.52535848-49
TCGGTGG388250.016.47108338-39
TATGGCG1281050.016.4641047
Produced by FastQC (version 0.11.5)