FastQCFastQC Report
Fri 27 May 2016
SRR1233058_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233058_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67436362
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTATTTAAAACCCATAAGTGAAATGGGCAGCTTGGGTCTCTGTCAAA923130.13688905697492995No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA864020.12812375614212404TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT488350.050.2333681
GCGTGTT593000.039.8407552
GTTTTTT6503450.035.7954981
CGTGTTT873750.027.104593
CTGGAAT1208300.021.5080811
AACGATC367600.018.17072138-39
CTCGTAT378750.018.13073544-45
TATGCCG395050.018.1056548-49
CGATCTC386300.017.58845740-41
CTTTATT1949550.017.4474181
CGCTTCG62250.017.3414444
GAAACGA422150.016.76250636-37
TGCCGTC428050.016.73134250-51
CGTATGC434600.016.48656546-47
TATGGCG1281600.016.4595177
TGGCGAG1292450.016.0638779
CCGTCTT447400.016.02032752-53
ATGGCGA1330950.015.920598
ATCTCGT433550.015.809031542-43
CTTTTAT1084650.015.1511121
Produced by FastQC (version 0.11.5)