FastQCFastQC Report
Fri 27 May 2016
SRR1233057_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233057_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18133897
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT503410.27760717952682756No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338510.18667250619102999No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG302330.1667209204949162Illumina Single End PCR Primer 1 (100% over 50bp)
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT289990.15991598496451148No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA264780.14601384357703145No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC226880.12511375795285481Illumina Single End PCR Primer 1 (100% over 50bp)
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC208280.11485672384705836No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT204480.1127612007501752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT263550.075.140181
GCGTGTT358200.055.3118442
CGTGTTT487400.041.414163
ACCACCG22500.036.9718781
CCACCGA22900.036.7412382
GTTTTTT2782400.035.5865331
CGGCCTT22350.033.817041
CAAAACG182800.031.6366719
ACCGAGA26400.031.5146544
CGCCGTA105150.030.77946546-47
CGCGCGT5200.030.1666151
CCGTATC110350.029.40976148-49
GTCGCCG110950.029.27947244-45
CCCGAAA31950.026.0365331
CACCGAG31900.025.9306243
CGGGCCT21050.025.5177481
TCGGTGG128800.025.4984438-39
CGAGCTA32600.025.46195212-13
CCGAAAC37050.025.4034671
TCTCGGT130050.025.2153936-37
Produced by FastQC (version 0.11.5)