FastQCFastQC Report
Fri 27 May 2016
SRR1233057_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233057_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18133897
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC400960.22111077392796488TruSeq Adapter, Index 1 (100% over 50bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT376830.20780420226275687No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT301200.16609777810031676No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292740.16143248194251902No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA259370.14303048043120573No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC215450.11881064505880892No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT213230.11758641840747192No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG210720.11620227025663596TruSeq Adapter, Index 1 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT271300.077.163061
GCGTGTT367550.057.0612832
CGGTGGT25400.047.4338531
CGTGTTT509700.042.004843
CGCGCGT5850.039.0739171
GTTTTTT2757650.037.15731
CGGCCTT20250.029.6310541
CAAAACG171700.029.6247849
CCGAGAT25300.028.7986741
CCCGAAA28350.028.0520761
GCGCGTT7650.027.3161872
TATGCCG131600.027.12136546-47
TGCCGTC135800.026.12514348-49
CTCGTAT125050.026.11123742-43
ATCACGA141400.025.71119534-35
CGGGCTT22700.025.384061
GTGGTCG10750.025.1823623
CCGTCTT144750.024.95357950-51
CACGATC130200.024.87724536-37
CGATCTC130900.024.78049338-39
Produced by FastQC (version 0.11.5)