FastQCFastQC Report
Fri 27 May 2016
SRR1233056_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233056_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27895744
Sequences flagged as poor quality0
Sequence length101
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4154721.4893741496910784Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2041990.7320077213212166Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1517640.5440399797187699No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1493040.5352214301937959No Hit
CAAGCAGAAGACGGCATACGAGATCTGAAGAGCGTCGTGTAGGGAAAGAG1397370.5009258760045977Illumina Single End PCR Primer 1 (96% over 30bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1171210.4198525767945103No Hit
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGAGATC806820.28922691576177356Illumina Single End PCR Primer 1 (97% over 44bp)
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT644860.23116787994613086No Hit
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT628550.22532110991554838No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG582480.2088060458254851Illumina Single End PCR Primer 1 (98% over 50bp)
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT542540.19448844956420594Illumina Single End PCR Primer 1 (100% over 43bp)
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA480350.17219472619192375No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC395260.1416918652537104No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT393670.14112188583319377No Hit
CAAGCAGAAGACGGCATACGAGATCTGATCGTCGTGTAGGGAAAGAGTGT380190.1362896074756063Illumina PCR Primer Index 9 (96% over 32bp)
AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA332810.11930493769945695No Hit
ATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTT314860.11287026436720958No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA312570.11204935061061645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT706450.066.5106351
AGACGGC423850.065.4871759
AAGACGG451000.061.7879648
GAAGACG485550.057.434527
GAGCGGC95100.053.5351339
CAAGCAG526000.053.1394961
GCGTGTT916100.050.932312
ATCGGGA67750.046.231032
GCAGAAG606600.045.9468734
AGAAGAC613650.045.2359126
CCGAGAT309100.042.546331
ACCACCG147100.042.0355341
CCACCGA151350.040.6672442
CGTGTTT1170050.040.5534633
CGGCGAC32400.040.1936571
AAGCAGA700500.040.1807172
AGCAGAA696550.040.1260763
ATCGTCG59050.039.6355628-29
ACCGAGA160800.038.744234
AGAGCGG130750.038.604988
Produced by FastQC (version 0.11.5)