##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233056_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27895744
Sequences flagged as poor quality	0
Sequence length	101
%GC	32
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18924818782392	34.0	31.0	34.0	31.0	34.0
2	32.41234827793085	34.0	33.0	34.0	31.0	34.0
3	32.49773187623173	34.0	33.0	34.0	31.0	34.0
4	35.601123956399945	37.0	37.0	37.0	35.0	37.0
5	35.62724876597663	37.0	37.0	37.0	35.0	37.0
6	35.673841644087354	37.0	37.0	37.0	35.0	37.0
7	35.559470756542645	37.0	37.0	37.0	35.0	37.0
8	35.4130129312916	37.0	37.0	37.0	35.0	37.0
9	36.92579294533245	39.0	39.0	39.0	34.0	39.0
10-11	37.124341046433464	39.0	39.0	39.0	34.5	39.0
12-13	37.2404575407632	39.0	39.0	39.0	35.0	39.0
14-15	38.69903566651601	41.0	40.0	41.0	36.0	41.0
16-17	38.413976590837656	41.0	39.5	41.0	35.0	41.0
18-19	37.99871362097387	41.0	38.5	41.0	34.0	41.0
20-21	37.64516884726215	41.0	38.5	41.0	33.5	41.0
22-23	37.326599623942634	41.0	38.0	41.0	33.5	41.0
24-25	36.81297446305788	41.0	37.0	41.0	30.5	41.0
26-27	36.48166119892698	41.0	37.0	41.0	28.5	41.0
28-29	35.96197511347968	41.0	37.0	41.0	24.0	41.0
30-31	35.352231813569844	41.0	36.0	41.0	15.5	41.0
32-33	35.05321390603527	40.0	35.0	41.0	11.5	41.0
34-35	34.85230363456161	40.0	35.0	41.0	8.5	41.0
36-37	34.67933724943848	40.0	35.0	41.0	7.0	41.0
38-39	34.67579409246084	40.0	35.0	41.0	6.0	41.0
40-41	34.63609110049189	40.0	35.0	41.0	2.0	41.0
42-43	34.49927562426727	40.0	35.0	41.0	2.0	41.0
44-45	34.3675715191536	40.0	35.0	41.0	2.0	41.0
46-47	34.086828998000556	40.0	35.0	41.0	2.0	41.0
48-49	33.976400396418896	40.0	35.0	41.0	2.0	41.0
50-51	33.36109942075751	39.5	34.5	40.5	2.0	41.0
52-53	33.42970178533328	39.5	35.0	40.5	2.0	41.0
54-55	33.56653174405386	40.0	35.0	41.0	2.0	41.0
56-57	33.51089574811125	40.0	35.0	41.0	2.0	41.0
58-59	33.13659531719247	39.0	35.0	41.0	2.0	41.0
60-61	33.04228388746326	39.0	35.0	41.0	2.0	41.0
62-63	32.769600785696916	39.0	34.5	41.0	2.0	41.0
64-65	32.37009849961342	38.0	34.0	41.0	2.0	41.0
66-67	31.418769078179096	37.5	32.5	40.5	2.0	41.0
68-69	30.917685830498016	37.0	32.0	40.0	2.0	41.0
70-71	30.5385008731081	36.5	31.0	40.0	2.0	41.0
72-73	30.130039980292334	36.0	31.0	39.0	2.0	41.0
74-75	29.833133004088367	35.5	31.0	39.0	2.0	41.0
76-77	29.395568890365496	35.0	30.0	38.5	2.0	40.5
78-79	28.808722613743516	35.0	29.0	37.0	2.0	39.5
80-81	28.502106450360312	35.0	29.0	37.0	2.0	39.0
82-83	28.05286670253355	35.0	27.5	36.5	2.0	39.0
84-85	27.479373412661083	35.0	25.5	36.0	2.0	38.0
86-87	27.089818808919382	35.0	24.5	36.0	2.0	37.0
88-89	26.649900447179327	34.5	22.0	35.0	2.0	37.0
90-91	26.294211636728527	34.0	19.0	35.0	2.0	36.0
92-93	25.909587283278768	34.0	11.0	35.0	2.0	36.0
94-95	25.410366936260957	34.0	2.0	35.0	2.0	36.0
96-97	24.83011516021942	34.0	2.0	35.0	2.0	35.0
98-99	24.232550259279694	33.5	2.0	35.0	2.0	35.0
100-101	22.693668682936007	31.0	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.09036390063733535
1101	2	0.10430240492735976
1101	3	0.10133810577815439
1101	4	0.16652613433743113
1101	5	0.10052279939220199
1101	6	0.057161644561141145
1101	7	-0.04238989047271957
1101	8	-0.05299433409062004
1101	9	0.09443365120591096
1101	10-11	0.057538303559987014
1101	12-13	0.014866249401528364
1101	14-15	0.05684268458139741
1101	16-17	0.13661255441191855
1101	18-19	0.18057849622012156
1101	20-21	0.15181371420764833
1101	22-23	0.18958039762561896
1101	24-25	0.20114853657943854
1101	26-27	0.17065089406035128
1101	28-29	0.14355577446679035
1101	30-31	0.214258474906714
1101	32-33	0.22494486971046257
1101	34-35	0.20562335740532944
1101	36-37	0.14337237576578588
1101	38-39	0.10146021973002206
1101	40-41	0.07662187731791192
1101	42-43	0.15917129553848497
1101	44-45	0.18998937074292854
1101	46-47	0.17340591878601685
1101	48-49	0.13419649547679313
1101	50-51	0.021534717817488058
1101	52-53	0.06079558016240583
1101	54-55	0.03896474475794065
1101	56-57	0.039183481807242515
1101	58-59	0.1792041186248028
1101	60-61	0.037989760915401405
1101	62-63	0.02560117596302547
1101	64-65	-0.038433172677443395
1101	66-67	0.22040787513338955
1101	68-69	0.40468219857146437
1101	70-71	0.3432253929893996
1101	72-73	0.301649752946588
1101	74-75	0.2630972126695674
1101	76-77	0.3145256023892742
1101	78-79	0.42555471262569
1101	80-81	0.3023218636816978
1101	82-83	0.320773929670672
1101	84-85	0.44188015209888576
1101	86-87	0.40029017868530303
1101	88-89	0.4405469779934954
1101	90-91	0.41701160103709256
1101	92-93	0.41005812425218835
1101	94-95	0.5606017119430469
1101	96-97	0.5594959676937279
1101	98-99	0.5664544031013286
1101	100-101	0.5987098085402849
1102	1	0.14508503701078013
1102	2	0.12453652322597719
1102	3	0.11638503591983351
1102	4	0.22788538629301058
1102	5	0.13572169074774365
1102	6	0.14076393477328963
1102	7	0.07971823283957491
1102	8	0.032260482309808935
1102	9	0.18497137410145115
1102	10-11	0.16401219929137056
1102	12-13	0.14300418934375614
1102	14-15	0.14925284578153253
1102	16-17	0.23403288724042426
1102	18-19	0.25013797610350963
1102	20-21	0.26272447183608705
1102	22-23	0.33845198921700614
1102	24-25	0.35046354135482005
1102	26-27	0.3076274852800083
1102	28-29	0.23416846307735284
1102	30-31	0.2527567874441772
1102	32-33	0.26686603358010075
1102	34-35	0.22578606726793993
1102	36-37	0.1722100970830951
1102	38-39	0.20888235801229627
1102	40-41	0.19859903719845562
1102	42-43	0.27146208764234103
1102	44-45	0.2830711132530581
1102	46-47	0.27203387576884097
1102	48-49	0.26364144591907035
1102	50-51	0.1514384692193005
1102	52-53	0.20217778364032313
1102	54-55	0.2029709714746062
1102	56-57	0.24937060204569406
1102	58-59	0.36315904786169284
1102	60-61	0.28432546550438786
1102	62-63	0.2553398905170283
1102	64-65	0.23858404451411275
1102	66-67	0.45954981586034904
1102	68-69	0.5523670642746126
1102	70-71	0.4813288535907354
1102	72-73	0.5106254875024341
1102	74-75	0.3771951785796155
1102	76-77	0.3776796268040208
1102	78-79	0.5085162265067602
1102	80-81	0.40744528347234166
1102	82-83	0.4309769790574336
1102	84-85	0.5364982934934766
1102	86-87	0.4615510074339326
1102	88-89	0.5029189953166657
1102	90-91	0.4597144669960471
1102	92-93	0.5284007390362397
1102	94-95	0.6492308521451093
1102	96-97	0.6705959259802903
1102	98-99	0.73311914074716
1102	100-101	0.9107552554791596
1103	1	0.10378256575211964
1103	2	0.11285186082312038
1103	3	0.12676513205398265
1103	4	0.2261146451677547
1103	5	0.14977434407552437
1103	6	0.09105361350015073
1103	7	0.03217368950781463
1103	8	0.0354112894398142
1103	9	0.14698942052443442
1103	10-11	0.0989036661531415
1103	12-13	0.10687056978517262
1103	14-15	0.08947803424022283
1103	16-17	0.1523161043664416
1103	18-19	0.1952961309195942
1103	20-21	0.22598461041270212
1103	22-23	0.3024707655022283
1103	24-25	0.23305622462893893
1103	26-27	0.1955779270927991
1103	28-29	0.17363340350767942
1103	30-31	0.1946114370013703
1103	32-33	0.2890819406848948
1103	34-35	0.2605128443542526
1103	36-37	0.22247513968447663
1103	38-39	0.22517173168763094
1103	40-41	0.23196466016475625
1103	42-43	0.22173276377738915
1103	44-45	0.22142096758224739
1103	46-47	0.2256863319748632
1103	48-49	0.2302566936726933
1103	50-51	0.11221270888810864
1103	52-53	0.16286014631525347
1103	54-55	0.1851719148436075
1103	56-57	0.24917228731145968
1103	58-59	0.35808184067824556
1103	60-61	0.2472984158125371
1103	62-63	0.21621689806047328
1103	64-65	0.2520829740759254
1103	66-67	0.3877142717106814
1103	68-69	0.4468125323754748
1103	70-71	0.2946468910697071
1103	72-73	0.3461303659654824
1103	74-75	0.28068353902297005
1103	76-77	0.27681202556183493
1103	78-79	0.45228962225080593
1103	80-81	0.340923492653026
1103	82-83	0.34739012478746645
1103	84-85	0.5042273540019941
1103	86-87	0.4163103025080801
1103	88-89	0.4157334618501771
1103	90-91	0.3811511868247841
1103	92-93	0.4143770850377564
1103	94-95	0.5163875782107006
1103	96-97	0.6007485527500727
1103	98-99	0.5655523226469477
1103	100-101	0.6959530036928214
1104	1	0.10419764563960854
1104	2	0.1287325863155786
1104	3	0.09912316052221115
1104	4	0.2058491102466391
1104	5	0.12557123417547444
1104	6	0.09042325805612705
1104	7	0.06625441675211619
1104	8	0.017144309273732006
1104	9	0.1994319676351779
1104	10-11	0.13960191510198428
1104	12-13	0.1146981433729124
1104	14-15	0.11124010748380186
1104	16-17	0.16810491186429743
1104	18-19	0.13068757956450128
1104	20-21	0.14423356873935234
1104	22-23	0.2451321648340894
1104	24-25	0.22217278307443422
1104	26-27	0.2140915496340412
1104	28-29	0.1910172371189489
1104	30-31	0.1234433178418044
1104	32-33	0.1883266683581013
1104	34-35	0.17878287206665533
1104	36-37	0.12337687059525848
1104	38-39	0.133485150367882
1104	40-41	0.11826012946771414
1104	42-43	0.1320844824414067
1104	44-45	0.12396528738211288
1104	46-47	0.11000773344451176
1104	48-49	0.15834698206679576
1104	50-51	0.05810087392815433
1104	52-53	0.10742818497947582
1104	54-55	0.13283484987133676
1104	56-57	0.1308231788123635
1104	58-59	0.2548215583903257
1104	60-61	0.1634103923679291
1104	62-63	0.16885255110962305
1104	64-65	0.18420974666804568
1104	66-67	0.24228845889008355
1104	68-69	0.3098037221099261
1104	70-71	0.20349554350053367
1104	72-73	0.1897818429024376
1104	74-75	0.1358636952134198
1104	76-77	0.16873170036070206
1104	78-79	0.3343614484406494
1104	80-81	0.24150597595565415
1104	82-83	0.20783742166145913
1104	84-85	0.3329385263622946
1104	86-87	0.23223048462264018
1104	88-89	0.24779921517614767
1104	90-91	0.22407281655783606
1104	92-93	0.21127871033063883
1104	94-95	0.3034460844792264
1104	96-97	0.33642380715451736
1104	98-99	0.298687280843037
1104	100-101	0.41140244250192737
1105	1	0.14934071348087485
1105	2	0.14252153729744776
1105	3	0.13863850395291877
1105	4	0.2422221547365737
1105	5	0.14065782205632615
1105	6	0.09544666577631489
1105	7	0.1266078927334533
1105	8	0.1533822244383174
1105	9	0.27932193104410175
1105	10-11	0.20951792447875306
1105	12-13	0.14196737134242454
1105	14-15	0.10946065801113747
1105	16-17	0.1754694726900965
1105	18-19	0.13610740783148856
1105	20-21	0.13859771944144939
1105	22-23	0.20066065422098234
1105	24-25	0.16289875340184778
1105	26-27	0.17037307981602368
1105	28-29	0.09129615625386833
1105	30-31	0.046069709096968836
1105	32-33	0.1604730613364751
1105	34-35	0.11572733721276052
1105	36-37	0.13386183198725377
1105	38-39	0.12326661876102918
1105	40-41	0.10738335898496842
1105	42-43	0.13706628853243075
1105	44-45	0.09030642294782609
1105	46-47	0.0871136595592219
1105	48-49	0.15249340273559397
1105	50-51	-0.013644529710475695
1105	52-53	0.04604535774439711
1105	54-55	0.12944842446311355
1105	56-57	0.1499722559094181
1105	58-59	0.2990098728080923
1105	60-61	0.1998837075227371
1105	62-63	0.18577982425559725
1105	64-65	0.19761704172498895
1105	66-67	0.2394792649939852
1105	68-69	0.26119492894991936
1105	70-71	0.14221178960622538
1105	72-73	0.17919877157484976
1105	74-75	0.1301063408340326
1105	76-77	0.08789887869300372
1105	78-79	0.2644134891465342
1105	80-81	0.14261267112391351
1105	82-83	0.1505082957267625
1105	84-85	0.3239110445588729
1105	86-87	0.19282213570919282
1105	88-89	0.14680351100677314
1105	90-91	0.12416436291423238
1105	92-93	0.16362772734132847
1105	94-95	0.2580946133085824
1105	96-97	0.2666314584763718
1105	98-99	0.28293341707004416
1105	100-101	0.3072482966621166
1106	1	0.09881317990988236
1106	2	0.06691629812566902
1106	3	0.06796982144007302
1106	4	0.1754433273702105
1106	5	0.06021024765077243
1106	6	0.02401668290953296
1106	7	0.04739856152266242
1106	8	0.06886410235227913
1106	9	0.2423750420440598
1106	10-11	0.13496238895046275
1106	12-13	0.055680957696161215
1106	14-15	0.017762533638638445
1106	16-17	0.10075293718864486
1106	18-19	0.03012616766798004
1106	20-21	0.034349091809779964
1106	22-23	0.08598973248576414
1106	24-25	0.06637852798533572
1106	26-27	-0.021015943404485427
1106	28-29	-0.04839175233129822
1106	30-31	-0.10785655283351048
1106	32-33	-0.03151877428899752
1106	34-35	-0.05299005620197761
1106	36-37	-0.05990040307218436
1106	38-39	-0.032465511789418144
1106	40-41	-0.12059749722578061
1106	42-43	-0.09712088181718315
1106	44-45	-0.12966265258329912
1106	46-47	-0.08741617593572215
1106	48-49	-0.0632282380937852
1106	50-51	-0.26541753748121977
1106	52-53	-0.24687591753495042
1106	54-55	-0.15778045307077804
1106	56-57	-0.14839685392306734
1106	58-59	-0.0935499159384463
1106	60-61	-0.09670414583089126
1106	62-63	-0.14522085295557474
1106	64-65	-0.08586920728659209
1106	66-67	-0.02699228574867263
1106	68-69	-0.009031122266918601
1106	70-71	-0.0987416643619703
1106	72-73	-0.07456502500696871
1106	74-75	-0.11233785922645367
1106	76-77	-0.09884079916025712
1106	78-79	0.09762784129469182
1106	80-81	0.006772025768228218
1106	82-83	-0.02356690856952781
1106	84-85	0.15373367006641203
1106	86-87	0.012320293140938077
1106	88-89	-0.06064859280738588
1106	90-91	-0.05076106803942082
1106	92-93	-0.08079970215624144
1106	94-95	-0.028413623193213056
1106	96-97	-0.05002569034792259
1106	98-99	-0.02635785767195742
1106	100-101	0.08704607512418505
1107	1	-0.06346330849545012
1107	2	-0.06331752229436916
1107	3	-0.07656618020659067
1107	4	0.061871301478049645
1107	5	-0.045489601025835213
1107	6	-0.06448297279816018
1107	7	-0.0012475681559536156
1107	8	0.003929072834743863
1107	9	0.12599487493751127
1107	10-11	0.025901319008575285
1107	12-13	-0.05049490477262708
1107	14-15	-0.09724608295827153
1107	16-17	-0.07127161823906647
1107	18-19	-0.11252842673697216
1107	20-21	-0.11274350616456985
1107	22-23	-0.07938723510839196
1107	24-25	-0.11191692088553395
1107	26-27	-0.1431079921757643
1107	28-29	-0.1888853133907844
1107	30-31	-0.23834524866730078
1107	32-33	-0.10001991696108803
1107	34-35	-0.14639896013571985
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2308	26-27	-0.5271419309240812
2308	28-29	-0.5700907990573683
2308	30-31	-0.6409886019798137
2308	32-33	-0.5788086790902298
2308	34-35	-0.6084725450916224
2308	36-37	-0.6100956652933505
2308	38-39	-0.6544021511120377
2308	40-41	-0.6177933224962331
2308	42-43	-0.6774489557767893
2308	44-45	-0.668062226729397
2308	46-47	-0.657291981697071
2308	48-49	-0.6362250883146103
2308	50-51	-0.5451448123935805
2308	52-53	-0.6274004159006665
2308	54-55	-0.748401019187753
2308	56-57	-0.6711286535834304
2308	58-59	-0.5930771357402662
2308	60-61	-0.6157861501712816
2308	62-63	-0.6495010555056666
2308	64-65	-0.695931024500478
2308	66-67	-0.4088743290151733
2308	68-69	-0.6553212827175621
2308	70-71	-0.6683125212125525
2308	72-73	-0.6641228355563946
2308	74-75	-0.685124369891831
2308	76-77	-0.7708166268820875
2308	78-79	-0.6203105924556063
2308	80-81	-0.8505395159043267
2308	82-83	-0.8312308952661738
2308	84-85	-0.8100976576234125
2308	86-87	-1.0911458326175456
2308	88-89	-1.2647430608932808
2308	90-91	-1.3091690603956287
2308	92-93	-1.532242676044973
2308	94-95	-1.701259789110658
2308	96-97	-2.0038226463524147
2308	98-99	-2.3228211008737603
2308	100-101	-2.7616642052400664
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	376359.0
3	78334.0
4	53780.0
5	47419.0
6	66035.0
7	107448.0
8	174728.0
9	313332.0
10	476211.0
11	483210.0
12	381334.0
13	291921.0
14	224348.0
15	192807.0
16	185774.0
17	187415.0
18	198047.0
19	199970.0
20	214626.0
21	232501.0
22	255376.0
23	261396.0
24	285106.0
25	400520.0
26	245531.0
27	259737.0
28	270232.0
29	293885.0
30	333521.0
31	363168.0
32	418228.0
33	526816.0
34	734760.0
35	1511332.0
36	2125761.0
37	4308821.0
38	7748610.0
39	3060244.0
40	7101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.327005330579137	17.82386624923988	9.804837341840459	42.04429107834052
2	15.62000092330687	21.19477847707065	50.61099796334012	12.574222636282355
3	17.32275010376953	23.315579946116305	40.170149360994	19.191520589120156
4	19.900196843967343	27.478201692061706	34.39503036247344	18.226571101497512
5	21.090787023371384	29.830384344731304	34.48403406413628	14.594794567761033
6	17.944150263327003	33.81760252162081	35.30659017558724	12.931657039464945
7	13.379785564547506	20.79575069460794	52.94654349577218	12.87792024507237
8	11.672994497992727	26.25972932338601	46.18085216454625	15.886424014075008
9	16.337356906687482	22.61892422801729	45.2420058832262	15.80171298206903
10-11	17.52796046008636	33.53968194496983	36.30265493115488	12.629702663788928
12-13	16.97268225996049	25.491403136098896	41.48835153265149	16.047563071289126
14-15	16.091967749680116	26.8666855311867	41.853205815786545	15.188140903346644
16-17	18.049242254127353	27.61739662798506	40.320958308438016	14.012402809449576
18-19	17.562331370829686	28.04181448266106	39.876726949373605	14.519127197135658
20-21	18.419508629982495	29.89876351655509	37.92280887274801	13.75891898071441
22-23	20.170701613312513	28.81814035946822	37.65193860461184	13.359219422607424
24-25	16.460465351715612	31.68198340819156	37.06898523674163	14.788566003351194
26-27	17.893735605749725	31.157765349323952	36.73576361997451	14.212735424951815
28-29	18.125314663376674	32.183838876399605	35.21806909627376	14.472777363949962
30-31	19.06199937525283	30.35505625841259	36.219340830838284	14.363603535496294
32-33	16.799183182581334	31.301500483634797	36.506916194175574	15.392400139608291
34-35	18.24314539800894	32.50649523916713	34.43716647268374	14.813192890140185
36-37	16.18502344546821	31.338298890443173	36.247925175827525	16.22875248826109
38-39	16.106142444421046	30.46411989401451	36.71411787192567	16.715619789638776
40-41	19.02824429153516	30.47006342506039	35.42699237371073	15.074699909693717
42-43	18.097892499774645	30.660621006124	36.668487183153516	14.57299931094784
44-45	18.917813883328623	30.217109308458056	35.993261028996336	14.871815779216982
46-47	16.929338880248775	31.807759021020736	34.76911965732289	16.493782441407596
48-49	16.139464619507933	30.727672632007387	35.27051826676167	17.862344481723017
50-51	17.81103185480518	30.342466703918802	36.97931445197858	14.86718698929744
52-53	16.34013470065373	32.60524231342053	36.675316638384224	14.37930634754151
54-55	16.191603448929477	33.330046641332075	34.164281484826	16.314068424912456
56-57	16.170074621533953	31.797726140501105	37.27384713133811	14.758352106626834
58-59	15.83124037772464	35.1743820584488	33.84234565795939	15.152031905867167
60-61	15.798470003479759	35.53576280203713	33.465615156272385	15.200152038210732
62-63	15.820688069952766	36.51621438961948	33.101745030035715	14.561352510392043
64-65	16.257694915759753	35.775970354132106	32.993642889375174	14.97269184073297
66-67	15.765772999918124	36.26085238632409	32.77659953997224	15.196775073785545
68-69	17.02123804030392	35.260596485335824	32.90376569727614	14.81439977708411
70-71	15.850811129604187	36.03635424163708	32.47472632636533	15.63810830239341
72-73	16.172254175851393	35.602863213578814	33.11669315848595	15.10818945208384
74-75	16.391760334479695	34.77172360056072	33.236708079913555	15.599807985046036
76-77	16.195860732016346	35.16282304070951	33.274659477896066	15.366656749378071
78-79	16.47243904240164	35.67741100574212	32.69050123281003	15.159648719046215
80-81	16.575714919093034	36.14894444113052	32.59386987491712	14.681470764859327
82-83	16.87626793535243	35.1897479414781	32.631963499521646	15.302020623647822
84-85	16.836295888003562	35.13738153031516	32.56290995500962	15.463412626671651
86-87	17.23607551030007	35.62090869488909	32.00586619951775	15.137149595293103
88-89	17.27591742329951	34.87930499234286	32.7033767429322	15.141400841425433
90-91	17.053227790386867	34.96200050635521	32.22207036544955	15.762701337808377
92-93	18.11484391492825	34.648410990773364	31.888626159781957	15.348118934516428
94-95	18.264029039054584	34.72365105786653	31.109633701626997	15.902686201451882
96-97	17.750935323259846	35.35602849047409	31.748373160117776	15.144663026148292
98-99	18.242290810408946	34.790239662571985	31.237670834287712	15.729798692731359
100-101	18.51279086557661	35.46675861926562	30.33608930634483	15.68436120881294
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	170622.0
1	176980.5
2	160721.0
3	157283.5
4	192350.0
5	223998.5
6	224656.0
7	204438.5
8	198424.5
9	194737.5
10	191972.0
11	189527.5
12	192550.5
13	195083.5
14	214956.0
15	226496.5
16	213179.5
17	215221.5
18	227281.0
19	245717.0
20	273932.5
21	312105.5
22	361602.0
23	412553.5
24	463630.0
25	537436.5
26	623300.5
27	705108.5
28	774945.0
29	837654.5
30	891127.5
31	915597.0
32	943068.0
33	975338.5
34	1003968.0
35	1022694.0
36	1043152.5
37	1060806.0
38	1058200.0
39	1036674.5
40	1022509.5
41	984687.5
42	843858.5
43	713837.0
44	648545.0
45	593931.0
46	541658.5
47	488603.0
48	433831.0
49	374146.0
50	313383.5
51	259592.5
52	220734.0
53	196623.0
54	181401.5
55	191397.5
56	169606.0
57	114591.0
58	87891.0
59	68690.5
60	51169.0
61	37628.5
62	29420.0
63	23366.0
64	18186.0
65	14368.5
66	11959.0
67	10401.0
68	8992.0
69	7826.0
70	7017.0
71	6409.0
72	5795.5
73	5190.5
74	4640.0
75	4045.5
76	3393.5
77	2813.5
78	2349.5
79	1888.5
80	1459.0
81	1133.5
82	887.0
83	662.0
84	452.0
85	315.0
86	225.5
87	151.5
88	103.0
89	69.5
90	48.0
91	31.5
92	18.0
93	10.0
94	5.5
95	5.5
96	4.5
97	3.0
98	1.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11934795501421293
2	0.14106094463728946
3	0.13742956631663955
4	0.07482861901801222
5	0.08760117672430605
6	0.14569964507847505
7	0.14504721580467617
8	0.14893311323763223
9	0.1504853213450769
10-11	0.15950282595079737
12-13	0.17238830410832562
14-15	0.19126035856939322
16-17	0.22417039674582617
18-19	0.23593921710781401
20-21	0.2536067867557144
22-23	0.2652017454705636
24-25	0.2514559210179158
26-27	0.24769011358865353
28-29	0.22023252005754
30-31	0.22255903983059208
32-33	0.26389150975862125
34-35	0.2750043160705805
36-37	0.28726245838791753
38-39	0.27952292650807237
40-41	0.28227424226433967
42-43	0.2815716261233255
44-45	0.2897036910003189
46-47	0.28823393274615655
48-49	0.0440389759814257
50-51	0.02976439703490253
52-53	0.006518915573644496
54-55	0.0015109831808034946
56-57	6.345053926505778E-4
58-59	6.631836024878921E-4
60-61	4.4809702870803514E-4
62-63	4.3375792378937806E-4
64-65	1.8640836394254263E-4
66-67	1.290519442679141E-4
68-69	7.348791270811777E-5
70-71	6.0941195904292786E-5
72-73	9.141179385643917E-5
74-75	0.0
76-77	5.197925533013208E-5
78-79	4.445122524783709E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	8.029898754447991E-4
90-91	0.0010987339143921021
92-93	3.262146368994496E-4
94-95	2.509343360764997E-4
96-97	6.255434520764171E-4
98-99	9.087407742198953E-4
100-101	7.599725606888276E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.7895744E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.56477554433667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.14068148748302	30.520426179906234
2	10.329546595740368	7.967132918789886
3	3.4951527309241164	4.043693416837917
4	1.7802944631673305	2.746266254994938
5	1.0756299041740245	2.074071291162381
6	0.6847304519638978	1.5843885713015955
7	0.5228600757977694	1.4114787024965083
8	0.397854208111687	1.2274526588157615
9	0.2955707937496034	1.0258759186573394
>10	1.9376699380070639	14.202726709570552
>50	0.1887671038271562	5.049405265021227
>100	0.13053323565139968	9.808526113934125
>500	0.011824695133413372	3.1380558135851637
>1k	0.00781750209982756	5.829275893111967
>5k	7.050250161665564E-4	1.8413445782513793
>10k+	3.617891530290017E-4	7.5298797135630995
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	415472	1.4893741496910784	Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	204199	0.7320077213212166	Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	151764	0.5440399797187699	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	149304	0.5352214301937959	No Hit
CAAGCAGAAGACGGCATACGAGATCTGAAGAGCGTCGTGTAGGGAAAGAG	139737	0.5009258760045977	Illumina Single End PCR Primer 1 (96% over 30bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	117121	0.4198525767945103	No Hit
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGAGATC	80682	0.28922691576177356	Illumina Single End PCR Primer 1 (97% over 44bp)
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	64486	0.23116787994613086	No Hit
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62855	0.22532110991554838	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG	58248	0.2088060458254851	Illumina Single End PCR Primer 1 (98% over 50bp)
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT	54254	0.19448844956420594	Illumina Single End PCR Primer 1 (100% over 43bp)
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA	48035	0.17219472619192375	No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC	39526	0.1416918652537104	No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT	39367	0.14112188583319377	No Hit
CAAGCAGAAGACGGCATACGAGATCTGATCGTCGTGTAGGGAAAGAGTGT	38019	0.1362896074756063	Illumina PCR Primer Index 9 (96% over 32bp)
AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA	33281	0.11930493769945695	No Hit
ATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTT	31486	0.11287026436720958	No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA	31257	0.11204935061061645	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.8517428321682332	0.0	0.0	3.584776229664281E-5	0.0
2	0.8578692147447295	3.5847762296642814E-6	0.0	5.018686721529994E-5	0.0
3	0.8581488272906433	3.5847762296642814E-6	0.0	6.452597213395707E-5	0.0
4	1.2815718412098993	3.5847762296642814E-6	0.0	7.886507705261419E-5	0.0
5	1.2884366876897064	3.5847762296642814E-6	0.0	1.0754328688992844E-4	0.0
6	1.2914801627086914	3.5847762296642814E-6	0.0	1.2905194426791414E-4	0.0
7	1.3112358645103712	7.169552459328563E-6	0.0	2.0791702132052833E-4	0.0
8	1.539776103480158	1.0754328688992845E-5	0.0	2.1867135000952116E-4	0.0
9	1.5422245056450188	1.0754328688992845E-5	0.0	2.7244299345448536E-4	0.0
10-11	1.6451165453769578	1.0754328688992845E-5	0.0	2.7602776968414967E-4	0.0
12-13	1.7041972424180547	1.9716269263153548E-5	0.0	2.9215926271763894E-4	0.0
14-15	1.728337125548614	2.150865737798569E-5	0.0	3.2621463689944965E-4	0.0
16-17	1.747664446590849	2.3301045492817832E-5	0.0	3.6027001108126025E-4	0.0
18-19	1.7589708308192102	2.509343360764997E-5	0.0	4.33757923789378E-4	0.0
20-21	1.7815710525591286	2.509343360764997E-5	0.0	5.126230008419923E-4	0.0
22-23	1.8020437096067414	2.509343360764997E-5	0.0	5.341316582199778E-4	0.0
24-25	1.8055299044900899	2.509343360764997E-5	0.0	6.434673332247385E-4	1.7923881148321407E-6
26-27	1.8093243901291896	2.509343360764997E-5	0.0	8.711006238084204E-4	3.5847762296642814E-6
28-29	1.813450467569533	2.509343360764997E-5	0.0	9.625124176648595E-4	3.5847762296642814E-6
30-31	1.8186250920570535	3.0470597952146393E-5	0.0	0.0010252460016839844	3.5847762296642814E-6
32-33	1.8243517720839422	3.2262986066978535E-5	0.0	0.001043169882832306	3.5847762296642814E-6
34-35	1.8289116074480751	3.584776229664281E-5	0.0	0.00107184809266962	3.5847762296642814E-6
36-37	1.8380061847427336	3.9432538526307096E-5	0.0	0.0011919380963633735	3.5847762296642814E-6
38-39	1.8519724729335056	4.122492664113924E-5	0.0	0.0012044848131671985	3.5847762296642814E-6
40-41	1.8665033633804495	4.301731475597138E-5	0.0	0.001249294516038002	3.5847762296642814E-6
42-43	1.9082660064560386	4.301731475597138E-5	0.0	0.001337121533664777	3.5847762296642814E-6
44-45	1.9223380455455858	4.301731475597138E-5	0.0	0.0014106094463728948	3.5847762296642814E-6
46-47	2.002599392939654	4.301731475597138E-5	0.0	0.0015253222857221516	3.5847762296642814E-6
48-49	2.030897616496624	4.301731475597138E-5	0.0	0.0015665472123632912	3.5847762296642814E-6
50-51	2.073154958691907	4.301731475597138E-5	0.0	0.001589848257856109	3.5847762296642814E-6
52-53	2.1730393711671576	4.301731475597138E-5	0.0	0.0016059797508895981	3.5847762296642814E-6
54-55	2.208876379135111	4.301731475597138E-5	0.0	0.0016274884082675837	3.5847762296642814E-6
56-57	2.230155610834398	4.660209098563566E-5	0.0	0.0016382427369565767	3.5847762296642814E-6
58-59	2.2593052187459133	5.1979255330132076E-5	0.0	0.001668713334908723	3.5847762296642814E-6
60-61	2.2847105278855437	5.73564196746285E-5	0.0	0.0017117306496646943	3.5847762296642814E-6
62-63	2.3403480473580487	6.0941195904292786E-5	0.0	0.001770879457454155	3.5847762296642814E-6
64-65	2.3759394981542705	6.452597213395707E-5	0.0	0.0018461597582771047	3.5847762296642814E-6
66-67	2.4216973743378203	7.707268893778205E-5	0.0	0.0018784227443440834	3.5847762296642814E-6
68-69	2.4628452282900217	9.320418197127131E-5	0.0	0.0018873846849182443	3.5847762296642814E-6
70-71	2.494317771198359	9.320418197127131E-5	0.0	0.0019071009541813976	3.5847762296642814E-6
72-73	2.5387600345056223	9.499657008610346E-5	0.0	0.0019196476709852227	3.5847762296642814E-6
74-75	2.5985505172401924	9.67889582009356E-5	0.0	0.001980588866889515	3.5847762296642814E-6
76-77	2.6424425173962023	9.67889582009356E-5	0.0	0.002163412454602394	3.5847762296642814E-6
78-79	2.6882129402965558	9.67889582009356E-5	0.0	0.002215391709932526	3.5847762296642814E-6
80-81	2.740616991609903	9.67889582009356E-5	0.0	0.0022745405177219866	3.5847762296642814E-6
82-83	2.801024055855976	9.67889582009356E-5	0.0	0.0023121806681334614	3.5847762296642814E-6
84-85	2.87316050792551	9.858134631576774E-5	0.0	0.00231935022059279	3.5847762296642814E-6
86-87	2.9615467506441124	1.0037373443059988E-4	0.0	0.0023247273849372864	3.5847762296642814E-6
88-89	3.0654174342867497	1.0216612254543202E-4	0.0	0.002331896937396615	3.5847762296642814E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	70645	0.0	66.510635	1
AGACGGC	42385	0.0	65.487175	9
AAGACGG	45100	0.0	61.787964	8
GAAGACG	48555	0.0	57.43452	7
GAGCGGC	9510	0.0	53.535133	9
CAAGCAG	52600	0.0	53.139496	1
GCGTGTT	91610	0.0	50.93231	2
ATCGGGA	6775	0.0	46.23103	2
GCAGAAG	60660	0.0	45.946873	4
AGAAGAC	61365	0.0	45.235912	6
CCGAGAT	30910	0.0	42.54633	1
ACCACCG	14710	0.0	42.035534	1
CCACCGA	15135	0.0	40.667244	2
CGTGTTT	117005	0.0	40.553463	3
CGGCGAC	3240	0.0	40.193657	1
AAGCAGA	70050	0.0	40.180717	2
AGCAGAA	69655	0.0	40.126076	3
ATCGTCG	5905	0.0	39.63556	28-29
ACCGAGA	16080	0.0	38.74423	4
AGAGCGG	13075	0.0	38.60498	8
>>END_MODULE