FastQCFastQC Report
Fri 27 May 2016
SRR1233056_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233056_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27895744
Sequences flagged as poor quality0
Sequence length101
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC6214592.2277914509109347TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1811680.6494467399758185TruSeq Adapter, Index 9 (100% over 49bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1298960.46564809312847155No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1106240.39656228563038143No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT800810.2870724652477453No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT583730.20925414285419308No Hit
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480550.17226642171651704No Hit
AGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTC472060.16922294669753207TruSeq Adapter, Index 9 (100% over 50bp)
CGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC471350.1689684275852259TruSeq Adapter, Index 9 (100% over 42bp)
ACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTT436500.15647548242484588TruSeq Adapter, Index 9 (100% over 50bp)
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA434890.15589833345186993No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT357520.12816291976295738No Hit
CACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCT351320.12594035850056554TruSeq Adapter, Index 9 (100% over 50bp)
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC346920.12436305695951325No Hit
GATGGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC336070.1204735747503275TruSeq Adapter, Index 9 (98% over 50bp)
AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA296820.1064033280488952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT109000.086.454781
CGGAGAT85600.077.16481
GTGGTCG52800.074.282863
CGCGTGT615450.072.349561
GTCGCCG25850.069.3883746
GGTCGCC27450.067.412825
CGCCGTA10300.061.5709238
CCGAGAT113900.057.2189331
GCGTGTT781650.056.550662
GGTGGTC71100.056.4718742
GGTAGAT106300.055.919655
GGTCGAG31000.054.0442665
TCGGAAG1492550.048.7823643
TCGCCGA27350.048.278637
GATCGGA1531850.047.8883481
CGCCGAG29550.047.72738
ATCGGAA1528900.047.7239532
CGGAAGA1530450.047.4527744
GTGGTAG115100.046.6916053
TCGGCAC35200.046.6546447
Produced by FastQC (version 0.11.5)