##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233056_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27895744
Sequences flagged as poor quality	0
Sequence length	101
%GC	33
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.500237742359552	34.0	31.0	34.0	28.0	34.0
2	31.853960123809568	34.0	31.0	34.0	29.0	34.0
3	32.11876919289193	34.0	31.0	34.0	30.0	34.0
4	30.832134213735255	37.0	35.0	37.0	2.0	37.0
5	32.548812069683464	37.0	35.0	37.0	19.0	37.0
6	34.45028108947372	37.0	35.0	37.0	32.0	37.0
7	34.810660185295646	37.0	35.0	37.0	32.0	37.0
8	34.52549460591551	37.0	35.0	37.0	31.0	37.0
9	36.350946043955666	39.0	37.0	39.0	32.0	39.0
10-11	36.65433216264101	39.0	38.0	39.0	32.5	39.0
12-13	36.78958426418023	39.0	39.0	39.0	34.0	39.0
14-15	38.22377824015018	41.0	40.0	41.0	33.5	41.0
16-17	38.06654651333193	41.0	39.5	41.0	33.0	41.0
18-19	37.768324963836776	41.0	39.0	41.0	33.0	41.0
20-21	37.37809753344453	41.0	38.5	41.0	32.5	41.0
22-23	36.84896975682025	40.5	37.0	41.0	31.5	41.0
24-25	36.48728029981921	41.0	37.0	41.0	29.5	41.0
26-27	35.97141320195654	40.0	37.0	41.0	25.5	41.0
28-29	35.59382714438446	40.0	36.0	41.0	21.0	41.0
30-31	35.11800126212802	40.0	36.0	41.0	11.0	41.0
32-33	34.73990650688506	40.0	35.5	41.0	6.0	41.0
34-35	34.45904393157609	40.0	35.0	41.0	2.0	41.0
36-37	34.264072523034336	40.0	35.0	41.0	2.0	41.0
38-39	34.02780599793287	40.0	35.0	41.0	2.0	41.0
40-41	33.77317783673381	40.0	35.0	41.0	2.0	41.0
42-43	33.25295136419376	39.0	35.0	41.0	2.0	41.0
44-45	33.261048495426394	39.0	35.0	40.0	2.0	41.0
46-47	33.02274149777113	39.0	34.5	40.0	2.0	41.0
48-49	32.76076400758481	38.0	34.0	40.0	2.0	41.0
50-51	32.4247901041822	38.0	33.5	40.0	2.0	41.0
52-53	32.13749929021431	38.0	33.0	40.0	2.0	41.0
54-55	31.80373314294826	38.0	33.0	40.0	2.0	41.0
56-57	31.513711016275458	38.0	33.0	40.0	2.0	40.5
58-59	31.01686633631281	37.0	31.5	39.0	2.0	40.0
60-61	30.52274167700994	36.0	31.0	39.0	2.0	40.0
62-63	30.05384090132172	36.0	30.5	38.5	2.0	40.0
64-65	29.386643424889474	35.0	29.0	38.0	2.0	40.0
66-67	28.83962069626105	35.0	28.5	38.0	2.0	39.5
68-69	28.0990244784294	34.0	27.0	37.0	2.0	39.0
70-71	27.208901347101552	34.0	25.5	36.0	2.0	39.0
72-73	26.363788648189484	33.0	21.5	36.0	2.0	38.0
74-75	25.09261179769932	31.5	11.0	35.0	2.0	37.5
76-77	24.301105035951004	30.5	12.5	34.0	2.0	36.0
78-79	24.355446139023933	31.0	8.5	34.0	2.0	36.0
80-81	23.844607926571165	30.5	3.5	34.0	2.0	36.0
82-83	23.239716639212062	30.0	2.0	34.0	2.0	35.0
84-85	22.661356657130206	29.0	2.0	33.5	2.0	35.0
86-87	21.715707779652696	29.0	2.0	32.5	2.0	34.5
88-89	20.864024938714664	27.5	2.0	32.0	2.0	34.0
90-91	19.910946899283275	26.0	2.0	31.5	2.0	34.0
92-93	18.637265670347418	25.5	2.0	31.0	2.0	34.0
94-95	17.093774358554484	22.0	2.0	30.5	2.0	33.5
96-97	15.3436164133138	10.0	2.0	29.5	2.0	32.5
98-99	13.560163908874415	2.0	2.0	29.0	2.0	32.5
100-101	8.799818997478612	2.0	2.0	19.0	2.0	27.5
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.2604345786298161
1101	2	0.3491255866277605
1101	3	0.3216037873549453
1101	4	4.789539475990363
1101	5	2.9650224237958867
1101	6	1.009486874788017
1101	7	0.39476385540478987
1101	8	0.19431796594137296
1101	9	0.14192899956682226
1101	10-11	0.178317123431583
1101	12-13	0.176746314543486
1101	14-15	0.028356372308643074
1101	16-17	0.02010458901894907
1101	18-19	0.08471127154393088
1101	20-21	0.17601852224277792
1101	22-23	0.20298748722795068
1101	24-25	0.047452602446597325
1101	26-27	0.07565711895528437
1101	28-29	0.0967304169878318
1101	30-31	0.15053523629398313
1101	32-33	0.08163362331561785
1101	34-35	0.028031831148432218
1101	36-37	0.046555030790905505
1101	38-39	-0.006184743043853302
1101	40-41	-0.0475228613540466
1101	42-43	0.0015814947850785188
1101	44-45	-0.17723997577401462
1101	46-47	-0.17458102100157902
1101	48-49	-0.2811796253978258
1101	50-51	-0.3057900124464652
1101	52-53	-0.3482836814067589
1101	54-55	-0.434034826469194
1101	56-57	-0.47313475739377253
1101	58-59	-0.5111541019302202
1101	60-61	-0.5650425887466
1101	62-63	-0.6971992078700673
1101	64-65	-0.7749206831901212
1101	66-67	-0.7138811074108844
1101	68-69	-0.7909981177557803
1101	70-71	-0.9290550379125335
1101	72-73	-0.8947335326651213
1101	74-75	-0.659553748445866
1101	76-77	-0.9178746164427238
1101	78-79	-0.9310437018311113
1101	80-81	-0.955746859770688
1101	82-83	-1.355100013082339
1101	84-85	-1.5990657630662461
1101	86-87	-1.7426337135258798
1101	88-89	-2.0929190740158923
1101	90-91	-2.5083936389746277
1101	92-93	-2.955107230219376
1101	94-95	-3.17397072067207
1101	96-97	-3.0416951732739133
1101	98-99	-2.769577250753768
1101	100-101	-1.2181635890627671
1102	1	0.0344816254524396
1102	2	0.11265485968780808
1102	3	0.1273473918089394
1102	4	4.598797446669796
1102	5	2.7976818597472786
1102	6	0.8516696514411493
1102	7	0.24799773631438882
1102	8	0.012645678858639542
1102	9	-0.10489827539463903
1102	10-11	-0.04628018547566626
1102	12-13	0.040876476771451564
1102	14-15	0.06702651932891968
1102	16-17	0.03736009065546142
1102	18-19	0.044255769726262884
1102	20-21	0.07338695969730935
1102	22-23	0.0991264219154786
1102	24-25	0.04455604660523704
1102	26-27	0.02238792900885045
1102	28-29	0.017254331775149012
1102	30-31	0.08459740163224438
1102	32-33	0.0023929337599994938
1102	34-35	-0.035528919890609245
1102	36-37	0.009984026290112524
1102	38-39	-0.024110607976417953
1102	40-41	-0.052878080817627904
1102	42-43	0.006543973337166165
1102	44-45	-0.04508579823765757
1102	46-47	-0.08422772072793094
1102	48-49	-0.14770277924864672
1102	50-51	-0.1424026440448003
1102	52-53	-0.20329588111311736
1102	54-55	-0.24237703023090162
1102	56-57	-0.22594541307683969
1102	58-59	-0.3090044775713565
1102	60-61	-0.4373761216975538
1102	62-63	-0.5329974263230852
1102	64-65	-0.5781021272532207
1102	66-67	-0.5538651494532054
1102	68-69	-0.6187022388426797
1102	70-71	-0.6726594572349782
1102	72-73	-0.6221766927206858
1102	74-75	-0.36812475073806894
1102	76-77	-0.5543877366518863
1102	78-79	-0.5819195752512201
1102	80-81	-0.6030149619558571
1102	82-83	-0.955520127934232
1102	84-85	-1.1630745731495615
1102	86-87	-1.240717248406849
1102	88-89	-1.4880333938857824
1102	90-91	-1.876369753765296
1102	92-93	-2.4050409807357482
1102	94-95	-2.5827460254489587
1102	96-97	-2.507387452386441
1102	98-99	-2.3777427381510154
1102	100-101	-0.9537377091125165
1103	1	0.09274861924254196
1103	2	0.10205352707905035
1103	3	0.09319743517941248
1103	4	4.580627666379289
1103	5	2.7622818856227056
1103	6	0.8215516907145783
1103	7	0.255733630533868
1103	8	0.034153847631117173
1103	9	-0.025717299706165875
1103	10-11	-0.002827415942867617
1103	12-13	0.056674320271916656
1103	14-15	0.027226851939879282
1103	16-17	0.0083185084591193
1103	18-19	0.02390664981837176
1103	20-21	0.022840575016395803
1103	22-23	0.08580399020699758
1103	24-25	0.059917995034737714
1103	26-27	0.04482128917711492
1103	28-29	0.03550250128499499
1103	30-31	0.022426832030824073
1103	32-33	-0.027829780054787534
1103	34-35	-0.03630864063625694
1103	36-37	-0.0451795279879974
1103	38-39	-0.06557404549621992
1103	40-41	-0.07107579285187882
1103	42-43	0.002132368575843202
1103	44-45	-0.06627395786547652
1103	46-47	-0.11136710894570001
1103	48-49	-0.1327051515508515
1103	50-51	-0.16136266925524723
1103	52-53	-0.2076466436247273
1103	54-55	-0.2119964988321037
1103	56-57	-0.23913229912573541
1103	58-59	-0.3228137278581862
1103	60-61	-0.37101503255914636
1103	62-63	-0.49810288425475946
1103	64-65	-0.542298334963558
1103	66-67	-0.49353186893824486
1103	68-69	-0.5352653270165995
1103	70-71	-0.5327346998455695
1103	72-73	-0.5798963790309166
1103	74-75	-0.34191337229884766
1103	76-77	-0.4853905732536177
1103	78-79	-0.5290164175887035
1103	80-81	-0.5178720178314791
1103	82-83	-0.8535988647878696
1103	84-85	-0.9812154648552678
1103	86-87	-1.0517785843443193
1103	88-89	-1.2541711788162893
1103	90-91	-1.565978969279218
1103	92-93	-2.1238384653464877
1103	94-95	-2.3366876564297563
1103	96-97	-2.2158819235558838
1103	98-99	-2.0464244154769613
1103	100-101	-0.6953862838489684
1104	1	0.15861280175191794
1104	2	0.1479527580061486
1104	3	0.11565392571934296
1104	4	4.636709128561922
1104	5	2.8062968191544897
1104	6	0.8579635000846224
1104	7	0.3364116063279212
1104	8	0.1638284647715551
1104	9	0.06098272572207719
1104	10-11	0.03752006665196461
1104	12-13	0.05388648904704496
1104	14-15	0.06654795918141332
1104	16-17	0.04810696700567263
1104	18-19	0.044349937393398875
1104	20-21	0.0694816566844807
1104	22-23	0.12496679129627353
1104	24-25	0.0610344691137783
1104	26-27	0.022635430817878444
1104	28-29	0.049688430401531036
1104	30-31	0.03786148301131931
1104	32-33	8.646727782135599E-4
1104	34-35	-0.013998717252256654
1104	36-37	-0.019547712425932673
1104	38-39	-0.04362121503204719
1104	40-41	-0.06320931689166542
1104	42-43	-0.01959652243812826
1104	44-45	-0.04446753013780835
1104	46-47	-0.07657063011110665
1104	48-49	-0.39399536163648463
1104	50-51	-0.33291656820838256
1104	52-53	-0.2413135104409747
1104	54-55	-0.23046747868844264
1104	56-57	-0.22448770721483768
1104	58-59	-0.29947708264597495
1104	60-61	-0.30622780284117823
1104	62-63	-0.4126403042730651
1104	64-65	-0.5245411103332991
1104	66-67	-0.4312351652072621
1104	68-69	-0.5518558376029041
1104	70-71	-0.5164815463359744
1104	72-73	-0.5745808817657121
1104	74-75	-0.34514670927304536
1104	76-77	-0.5496410470093807
1104	78-79	-0.6661889203503613
1104	80-81	-0.6717676596091309
1104	82-83	-0.8406140082327447
1104	84-85	-0.9395307917194806
1104	86-87	-1.0096673280231876
1104	88-89	-1.1964812972256134
1104	90-91	-1.4984225452330726
1104	92-93	-2.026461710147924
1104	94-95	-2.105851569031131
1104	96-97	-1.9738798773771773
1104	98-99	-1.862816500283616
1104	100-101	-0.7026752924933373
1105	1	0.20037275529962173
1105	2	0.19588802808953076
1105	3	0.17354204213726376
1105	4	4.671955858065264
1105	5	2.8373694983931586
1105	6	0.8732130188496399
1105	7	0.4195517189353666
1105	8	0.2737986383640276
1105	9	0.09395285057607339
1105	10-11	0.04335672744821295
1105	12-13	0.10599303009743011
1105	14-15	0.17111219433394353
1105	16-17	0.14438724529693303
1105	18-19	0.09367213696202015
1105	20-21	0.13650394842673563
1105	22-23	0.14615607426512156
1105	24-25	0.07157869100004888
1105	26-27	0.043488814727638214
1105	28-29	0.01300417087618655
1105	30-31	-0.010098828170661989
1105	32-33	-0.009727401019972604
1105	34-35	-0.029996128891930596
1105	36-37	-0.05808085289907439
1105	38-39	-0.060428641442307196
1105	40-41	-0.010765792699636734
1105	42-43	0.07731407720218897
1105	44-45	0.00884324150536031
1105	46-47	0.0027200680169627844
1105	48-49	-0.05675524285738476
1105	50-51	-0.12239412683872075
1105	52-53	-0.18298220936481613
1105	54-55	-0.16663751231541468
1105	56-57	-0.17527021874295556
1105	58-59	-0.29688105488548544
1105	60-61	-0.32395698895246383
1105	62-63	-0.3296646894967665
1105	64-65	-0.412789786631091
1105	66-67	-0.41578202497342076
1105	68-69	-0.44937008954187263
1105	70-71	-0.3976700877725321
1105	72-73	-0.4345978260534338
1105	74-75	-0.12460875437845687
1105	76-77	-0.37437585932600115
1105	78-79	-0.48151102713279315
1105	80-81	-0.4634999330344698
1105	82-83	-0.640491532960894
1105	84-85	-0.7349201065575102
1105	86-87	-0.7338618780255466
1105	88-89	-0.939317356657817
1105	90-91	-1.229695276399699
1105	92-93	-1.7319475781895335
1105	94-95	-1.8959414186635613
1105	96-97	-1.763202372080226
1105	98-99	-1.666704620166012
1105	100-101	-0.501187805939713
1106	1	0.2224408177339363
1106	2	0.20583445833816327
1106	3	0.17201092397804274
1106	4	4.693917821038287
1106	5	2.8354936870197065
1106	6	0.8878306127645317
1106	7	0.44498615216504334
1106	8	0.3537894937623207
1106	9	0.2392450512021611
1106	10-11	0.13232648481143627
1106	12-13	0.11302006350933169
1106	14-15	0.13827004696462808
1106	16-17	0.08543801594939282
1106	18-19	0.08560259772001189
1106	20-21	0.08475470817185027
1106	22-23	0.17494326170804442
1106	24-25	0.09623357237927621
1106	26-27	0.04015155182643326
1106	28-29	0.0821686872234011
1106	30-31	0.05060333152473362
1106	32-33	0.06034062524740591
1106	34-35	-0.016467056093354415
1106	36-37	0.007413161153259296
1106	38-39	-0.04365187697790418
1106	40-41	-0.009194103634584394
1106	42-43	0.0380627291900808
1106	44-45	0.03254373000834221
1106	46-47	-0.027884549053091234
1106	48-49	-0.02023061975062035
1106	50-51	-0.1617122452433435
1106	52-53	-0.21424266112656198
1106	54-55	-0.20075786768910575
1106	56-57	-0.16353771936288197
1106	58-59	-0.29026525802079206
1106	60-61	-0.27152363020582726
1106	62-63	-0.3417930286122619
1106	64-65	-0.4503786686229496
1106	66-67	-0.36065458637348513
1106	68-69	-0.4540795756549052
1106	70-71	-0.3434139271233789
1106	72-73	-0.3292507191781411
1106	74-75	-0.03832693150331323
1106	76-77	-0.3232034259091243
1106	78-79	-0.3486958763922523
1106	80-81	-0.3326590646270873
1106	82-83	-0.5078224741940751
1106	84-85	-0.5404264588596703
1106	86-87	-0.5704570031979124
1106	88-89	-0.6737759100224174
1106	90-91	-0.8741563866722295
1106	92-93	-1.3114806445268776
1106	94-95	-1.365040552451756
1106	96-97	-1.246514209640937
1106	98-99	-1.1409340580352154
1106	100-101	-0.3658230929192765
1107	1	0.319599060652763
1107	2	0.25167099007867577
1107	3	0.1995245247246089
1107	4	4.745022195469037
1107	5	2.8941557010367234
1107	6	0.8802222142351539
1107	7	0.47377003614725055
1107	8	0.47319203179668534
1107	9	0.26838869470595483
1107	10-11	0.14241229649758935
1107	12-13	0.08000779217676524
1107	14-15	0.09784289475596353
1107	16-17	0.04377085334636632
1107	18-19	0.022526687464086592
1107	20-21	-0.013278668757088496
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2308	22-23	-0.5782323837556405
2308	24-25	-0.145259447657196
2308	26-27	-0.20128126766369547
2308	28-29	-0.1361850257502084
2308	30-31	-0.06927274209947853
2308	32-33	0.07618234165970961
2308	34-35	0.02971238975859336
2308	36-37	0.05785915141580489
2308	38-39	-0.014666252713638528
2308	40-41	0.07092050061444155
2308	42-43	0.01006100411447619
2308	44-45	-0.07692145677768991
2308	46-47	0.02311841605577314
2308	48-49	0.07516342716757407
2308	50-51	0.14254543169439415
2308	52-53	0.010350903145820212
2308	54-55	0.12161483738035272
2308	56-57	0.1896690100891334
2308	58-59	0.17489917154779988
2308	60-61	0.13696223686183018
2308	62-63	0.16406315403690286
2308	64-65	0.252931802597427
2308	66-67	0.07236928243722573
2308	68-69	0.19720445154581867
2308	70-71	0.14834926135025128
2308	72-73	0.1845170467694217
2308	74-75	0.3663815756954989
2308	76-77	0.3867453371829477
2308	78-79	0.42008239334657205
2308	80-81	0.6165400863791533
2308	82-83	0.6555484847549913
2308	84-85	0.45707162803649837
2308	86-87	0.4233169650418809
2308	88-89	0.5291906090966627
2308	90-91	0.26727401382389004
2308	92-93	0.3386139280994449
2308	94-95	0.8870785067233307
2308	96-97	1.1176428933396263
2308	98-99	0.8984298398460755
2308	100-101	0.26739137703061466
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	579338.0
3	187564.0
4	94770.0
5	88383.0
6	193010.0
7	214845.0
8	151758.0
9	233963.0
10	417104.0
11	513620.0
12	396516.0
13	289726.0
14	224554.0
15	205153.0
16	203239.0
17	197721.0
18	199850.0
19	207060.0
20	208556.0
21	227381.0
22	260971.0
23	287427.0
24	316494.0
25	381106.0
26	485226.0
27	575118.0
28	467781.0
29	505786.0
30	680099.0
31	985551.0
32	1531064.0
33	2394074.0
34	3685741.0
35	4948510.0
36	4109070.0
37	1189216.0
38	58297.0
39	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.908974836794343	17.914991072782936	9.768757712241923	42.407276378180796
2	17.342702886863314	20.29542571081811	50.07799756120504	12.283873841113541
3	17.730392851325277	22.30069934682509	40.82896301313921	19.139944788710423
4	22.03222531464291	27.844103951991517	32.37192998442256	17.75174074894301
5	21.915507971395208	29.797004159487557	34.86729373484357	13.420194134273672
6	19.47024607051169	32.79878106136908	35.05456602985746	12.67640683826178
7	12.058972150016862	21.15027295920123	52.74550841877528	14.045246472006626
8	11.710116066450855	25.516670213205284	45.763178067593394	17.010035652750467
9	17.169583288404137	21.492020431503818	45.32628346460306	16.012112815488987
10-11	17.057474788985733	33.030165820277105	36.35194314946394	13.56041624127322
12-13	14.825469433616828	27.008266924158754	41.96176843320615	16.204495209018265
14-15	14.85903548584329	28.80317872145658	40.21873014033969	16.119055652360444
16-17	17.63844871819873	28.05408595662478	38.013255713846526	16.294209611329958
18-19	16.121012940181842	27.602665840351847	40.34410948136031	15.932211738106
20-21	17.95554547675803	28.258558007988604	38.51827361191729	15.267622903336079
22-23	16.156771802754815	32.97559862054814	36.909980234242404	13.957649342454637
24-25	15.548008399497162	29.700395768633665	37.52869952170974	17.222896310159435
26-27	15.870973005774644	29.460827429445867	35.953158302571175	18.715041262208317
28-29	17.715562632063158	31.822719981944196	34.78280772866284	15.678909657329806
30-31	16.48414539508249	30.48724923773318	36.31992213579247	16.70868323139186
32-33	15.895507820219173	31.978343569994315	33.91142950229289	18.214719107493625
34-35	17.440150629561643	32.49643551943516	33.51403429475981	16.549379556243387
36-37	15.722668513161997	31.818044618945073	35.14416669304466	17.31512017484827
38-39	17.491186110684122	32.46139805412611	33.95298221836277	16.094433616826997
40-41	15.859558644264146	32.51616403356944	36.07296120492137	15.551316117245046
42-43	15.965564496146795	31.33129376294821	35.63043523771942	17.072706503185575
44-45	16.934174618178314	30.5581991288707	34.609223901681915	17.89840235126907
46-47	15.668656580142212	32.719237117005704	36.23544020013335	15.376666102718739
48-49	16.69733741462497	31.514470451119713	36.3648429667264	15.423349167528924
50-51	15.83707894652317	31.03164411029869	34.888990592973606	18.242286350204534
52-53	17.95141581454146	31.425824312124455	34.9871525204705	15.635607352863575
54-55	15.619293402653897	32.21790726740832	34.85940632576403	17.303393004173756
56-57	16.299367257604196	32.11984681888646	35.44777360041774	16.133012323091606
58-59	16.694476945633035	33.26777332063693	34.065018445049375	15.972731288680661
60-61	16.15267878960558	32.56639234004559	33.867397015760794	17.41353185458804
62-63	16.9678015188022	33.09959594502128	34.7836354372841	15.148967098892422
64-65	15.658110865824112	36.179063026446926	32.14379425196745	16.01903185576152
66-67	16.009306880157524	36.847072714137234	32.13478575602601	15.008834649679226
68-69	15.741560532010531	37.054547935228925	32.17166666583021	15.032224866930333
70-71	15.50564953984394	37.2782877637955	31.25493256720986	15.9611301291507
72-73	15.715221052995346	37.06624294689625	31.528152583856283	15.690383416252121
74-75	16.00091683173868	35.602266030627646	31.76207323712444	16.634743900509235
76-77	15.299462942813982	36.15519651209024	32.46098606093292	16.084354484162855
78-79	15.64321182106824	36.20371358057018	31.631259887849534	16.52181471051205
80-81	16.20490379814518	36.15331675724721	31.56527324596219	16.07650619864542
82-83	16.035950796530834	35.46840089621539	32.393091752188575	16.1025565550652
84-85	16.447865860550603	35.78628844855619	31.513542135701073	16.252303555192128
86-87	15.939949744009683	36.58118501586493	31.06204883217756	16.416816407947827
88-89	16.298186867498185	35.811266084865295	31.552526383726885	16.338020663909635
90-91	15.851151200429427	36.279283397454485	30.876337656537267	16.993227745578814
92-93	16.4988972415326	35.94442089436756	30.91274714143526	16.64393472266458
94-95	16.782221264859356	35.87448518690309	30.131574810085947	17.211718738151603
96-97	15.961269153355193	36.35132572158735	30.33946496448083	17.347940160576623
98-99	16.80106336384128	36.00651406974639	30.001817172055432	17.1906053943569
100-101	17.26731872339176	35.60140486375603	28.19281212270395	18.93846429014826
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	97605.0
1	127724.0
2	142937.5
3	135250.0
4	156310.0
5	185284.5
6	196491.0
7	191225.0
8	190698.0
9	191283.0
10	191769.5
11	191733.0
12	195265.0
13	200126.0
14	212238.5
15	221696.0
16	218324.5
17	219060.5
18	227233.5
19	241997.5
20	265786.0
21	299918.0
22	344568.5
23	391865.5
24	442960.0
25	513632.0
26	598082.0
27	681367.5
28	753072.0
29	818502.5
30	872990.0
31	902263.5
32	932852.0
33	966516.5
34	995554.0
35	1020446.0
36	1053347.5
37	1071351.5
38	1059673.0
39	1041947.0
40	1031084.0
41	989421.0
42	868846.5
43	768549.5
44	728200.5
45	698132.0
46	662221.0
47	609730.0
48	536968.0
49	449941.0
50	366449.0
51	292744.5
52	230544.0
53	181538.0
54	145516.0
55	121638.0
56	104881.0
57	89813.5
58	74456.0
59	59005.5
60	43857.0
61	31985.5
62	24425.5
63	18764.5
64	13969.5
65	10390.5
66	7912.0
67	6115.5
68	4462.0
69	3153.0
70	2370.5
71	1753.5
72	1325.0
73	1033.5
74	755.5
75	494.0
76	332.5
77	229.0
78	150.5
79	99.5
80	71.0
81	48.0
82	29.0
83	25.0
84	24.5
85	24.5
86	20.5
87	16.0
88	17.5
89	14.0
90	10.0
91	11.0
92	11.0
93	8.5
94	6.0
95	3.0
96	0.5
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.24750370522471096
2	0.0
3	0.0
4	8.414394683289322
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	9.320418197127131E-5
24-25	9.499657008610346E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	3.7640150411474954E-5
34-35	8.78270176267749E-5
36-37	5.377164344496422E-5
38-39	0.0
40-41	5.377164344496422E-5
42-43	0.0
44-45	0.0
46-47	5.108306127271601E-4
48-49	0.0
50-51	0.0
52-53	0.0
54-55	4.4988941682286727E-4
56-57	9.625124176648595E-4
58-59	6.560140500285634E-4
60-61	8.083670397892954E-4
62-63	0.0014572115373585304
64-65	0.0010879795857031095
66-67	0.0016740904992532193
68-69	7.384639033108419E-4
70-71	5.502631512534673E-4
72-73	0.0020720006607459545
74-75	0.006660514234716235
76-77	0.0010664709283251237
78-79	0.0048197316407836265
80-81	0.004092022066161777
82-83	0.014380329845298264
84-85	0.01752417859871384
86-87	0.01842037265612991
88-89	0.03090256348782094
90-91	0.016714019170809714
92-93	0.013061132192781811
94-95	0.015453970326082719
96-97	0.013493097728456357
98-99	0.01255926352062881
100-101	0.008470826230696697
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.7895744E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.76522445362486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.59344112059672	33.66003157891721
2	9.594948278377023	8.014703369346755
3	3.3418765266343695	4.18722669693554
4	1.622031103530373	2.709779724388274
5	1.0114724125060488	2.112218616848228
6	0.6838293277646933	1.7136171217238292
7	0.4688848365203676	1.3708156308122046
8	0.35372912476745527	1.1818861033357633
9	0.29670291927830333	1.1152677617733668
>10	1.7391687499544732	13.798107359957415
>50	0.16712442938490618	4.8224715974474135
>100	0.11034678853525548	8.789822675693827
>500	0.00918190682737738	2.6437302674307244
>1k	0.006356381645025763	4.990117667116625
>5k	5.241130098501785E-4	1.4923134312185569
>10k+	3.819806681934467E-4	7.397890397054333
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC	621459	2.2277914509109347	TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG	181168	0.6494467399758185	TruSeq Adapter, Index 9 (100% over 49bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	129896	0.46564809312847155	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	110624	0.39656228563038143	No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	80081	0.2870724652477453	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	58373	0.20925414285419308	No Hit
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48055	0.17226642171651704	No Hit
AGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTC	47206	0.16922294669753207	TruSeq Adapter, Index 9 (100% over 50bp)
CGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC	47135	0.1689684275852259	TruSeq Adapter, Index 9 (100% over 42bp)
ACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTT	43650	0.15647548242484588	TruSeq Adapter, Index 9 (100% over 50bp)
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA	43489	0.15589833345186993	No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT	35752	0.12816291976295738	No Hit
CACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCT	35132	0.12594035850056554	TruSeq Adapter, Index 9 (100% over 50bp)
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC	34692	0.12436305695951325	No Hit
GATGGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC	33607	0.1204735747503275	TruSeq Adapter, Index 9 (98% over 50bp)
AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA	29682	0.1064033280488952	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.724379317504491	0.0	0.0	3.584776229664281E-5	0.0
2	0.7298747794645664	0.0	0.0	4.660209098563566E-5	0.0
3	0.7300038314088343	0.0	0.0	4.660209098563566E-5	3.5847762296642814E-6
4	0.801943120785737	0.0	0.0	6.452597213395707E-5	3.5847762296642814E-6
5	0.8084100571040514	0.0	0.0	8.961940574160703E-5	3.5847762296642814E-6
6	0.8109014765836681	0.0	0.0	1.14712839349257E-4	3.5847762296642814E-6
7	0.8269720284212531	0.0	0.0	1.8640836394254263E-4	3.5847762296642814E-6
8	1.0565948698123986	3.5847762296642814E-6	0.0	2.1150179755019262E-4	3.5847762296642814E-6
9	1.0587313964452785	3.5847762296642814E-6	0.0	2.6168866476549254E-4	3.5847762296642814E-6
10-11	1.13287353081531	3.5847762296642814E-6	0.0	2.6348105288032467E-4	3.5847762296642814E-6
12-13	1.180818120498955	3.5847762296642814E-6	0.0	2.885744864879747E-4	3.5847762296642814E-6
14-15	1.2075497968435616	3.5847762296642814E-6	0.0	3.3338418935877816E-4	3.5847762296642814E-6
16-17	1.2294742882641883	3.5847762296642814E-6	0.0	3.7281672788508526E-4	3.5847762296642814E-6
18-19	1.235179459633699	3.5847762296642814E-6	0.0	4.498894168228673E-4	3.5847762296642814E-6
20-21	1.2844342850292862	3.5847762296642814E-6	0.0	5.21584941416153E-4	3.5847762296642814E-6
22-23	1.3344472905974474	3.5847762296642814E-6	0.0	5.377164344496422E-4	3.5847762296642814E-6
24-25	1.3379155615996474	3.5847762296642814E-6	0.0	6.040347946984314E-4	3.5847762296642814E-6
26-27	1.341616843056776	3.5847762296642814E-6	0.0	8.01197487329967E-4	3.5847762296642814E-6
28-29	1.3454561383987462	3.5847762296642814E-6	0.0	9.05155997990231E-4	3.5847762296642814E-6
30-31	1.3506253857219224	3.5847762296642814E-6	0.0	9.786439106983487E-4	5.3771643444964225E-6
32-33	1.3559183078250217	5.3771643444964225E-6	0.0	9.876058512725094E-4	7.169552459328563E-6
34-35	1.3602092849719298	7.169552459328563E-6	0.0	0.0010001525680763345	7.169552459328563E-6
36-37	1.3662155775447324	7.169552459328563E-6	0.0	0.0010951491381624378	7.169552459328563E-6
38-39	1.3725534619187787	1.0754328688992845E-5	0.0	0.0011148654074255916	7.169552459328563E-6
40-41	1.3825729114806904	1.4339104918657126E-5	0.0	0.001174014215215052	7.169552459328563E-6
42-43	1.4045798527545994	1.7923881148321406E-5	0.0	0.0012743879496456521	7.169552459328563E-6
44-45	1.4121992946307507	2.3301045492817832E-5	0.0	0.001349668250468602	7.169552459328563E-6
46-47	1.420922847585639	2.509343360764997E-5	0.0	0.001467965866047523	7.169552459328563E-6
48-49	1.4325984637656555	2.509343360764997E-5	0.0	0.001502021240229334	7.169552459328563E-6
50-51	1.4650245571510836	2.509343360764997E-5	0.0	0.0015073984045738302	7.169552459328563E-6
52-53	1.5416867892105692	2.509343360764997E-5	0.0	0.0015217375094924875	7.169552459328563E-6
54-55	1.5656528106939898	2.509343360764997E-5	3.5847762296642814E-6	0.0015378690025259767	7.169552459328563E-6
56-57	1.5799632374028096	2.867820983731425E-5	3.5847762296642814E-6	0.0015719243767077875	7.169552459328563E-6
58-59	1.5974676997322603	2.867820983731425E-5	3.5847762296642814E-6	0.001629280796382416	7.169552459328563E-6
60-61	1.613679850230917	2.867820983731425E-5	3.5847762296642814E-6	0.0016758828873680515	7.169552459328563E-6
62-63	1.6443511956519246	2.867820983731425E-5	3.5847762296642814E-6	0.001745786023846505	7.169552459328563E-6
64-65	1.6746300080757839	3.2262986066978535E-5	3.5847762296642814E-6	0.0018318206533584477	7.169552459328563E-6
66-67	1.7083537904563506	4.122492664113924E-5	3.5847762296642814E-6	0.0018855922968034119	7.169552459328563E-6
68-69	1.7374191561264687	5.377164344496422E-5	5.3771643444964225E-6	0.0019088933422962298	7.169552459328563E-6
70-71	1.7681837057294474	5.377164344496422E-5	7.169552459328563E-6	0.001925024835329719	1.0754328688992845E-5
72-73	1.8224572178465648	5.556403155979636E-5	7.169552459328563E-6	0.0019304019996742156	1.4339104918657126E-5
74-75	1.8727641750655586	5.73564196746285E-5	7.169552459328563E-6	0.0020038899123823333	1.4339104918657126E-5
76-77	1.9163496768539314	5.73564196746285E-5	7.169552459328563E-6	0.002179543947635883	1.4339104918657126E-5
78-79	1.963948335631414	5.9148807789460644E-5	8.961940574160703E-6	0.00226916335337749	1.4339104918657126E-5
80-81	2.012864399673298	6.273358401912493E-5	1.2546716803824985E-5	0.002339066489855944	1.4339104918657126E-5
82-83	2.0933802661796728	6.452597213395707E-5	1.4339104918657126E-5	0.002371329475922922	1.4339104918657126E-5
84-85	2.172752589068784	6.452597213395707E-5	1.4339104918657126E-5	0.002382083804611915	1.4339104918657126E-5
86-87	2.2547005736789094	6.452597213395707E-5	1.4339104918657126E-5	0.002392838133300908	1.4339104918657126E-5
88-89	2.3482524789444583	6.452597213395707E-5	1.4339104918657126E-5	0.0024125544025640614	1.4339104918657126E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTGGT	10900	0.0	86.45478	1
CGGAGAT	8560	0.0	77.1648	1
GTGGTCG	5280	0.0	74.28286	3
CGCGTGT	61545	0.0	72.34956	1
GTCGCCG	2585	0.0	69.388374	6
GGTCGCC	2745	0.0	67.41282	5
CGCCGTA	1030	0.0	61.570923	8
CCGAGAT	11390	0.0	57.218933	1
GCGTGTT	78165	0.0	56.55066	2
GGTGGTC	7110	0.0	56.471874	2
GGTAGAT	10630	0.0	55.91965	5
GGTCGAG	3100	0.0	54.044266	5
TCGGAAG	149255	0.0	48.782364	3
TCGCCGA	2735	0.0	48.27863	7
GATCGGA	153185	0.0	47.888348	1
CGCCGAG	2955	0.0	47.7273	8
ATCGGAA	152890	0.0	47.723953	2
CGGAAGA	153045	0.0	47.452774	4
GTGGTAG	11510	0.0	46.691605	3
TCGGCAC	3520	0.0	46.654644	7
>>END_MODULE