FastQCFastQC Report
Fri 27 May 2016
SRR1233055_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233055_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35889287
Sequences flagged as poor quality0
Sequence length101
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC13324293.712609280869804Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8155262.272338260718303No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5747551.601466755246489No Hit
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGAGATC3528720.9832237681400581Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3345650.9322141172656898Illumina Single End PCR Primer 1 (100% over 50bp)
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT3096040.8626641147816617Illumina Single End PCR Primer 1 (100% over 43bp)
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2579490.7187353708085648No Hit
ATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTT1206970.33630369976422214No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT993520.27682912731033077No Hit
GCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT889190.2477591711420737No Hit
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC837500.2333565445309627Illumina Single End PCR Primer 1 (100% over 47bp)
GCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC833250.23217234714080556Illumina Single End PCR Primer 1 (100% over 40bp)
CGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT792420.22079569315489606No Hit
CGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG768040.21400257965559474Illumina Single End PCR Primer 1 (100% over 41bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT593980.16550342724835965No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT524630.14618011218779575No Hit
GTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT509150.14186684734082347No Hit
CGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTT488980.13624678584447777No Hit
CCGAGATCAGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGAGATC460170.12821932071261266Illumina Single End PCR Primer 1 (97% over 42bp)
CTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT433730.12085221977243515No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG423650.11804358219766249Illumina Single End PCR Primer 1 (98% over 50bp)
CGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC360490.10044501580652744Illumina Single End PCR Primer 1 (100% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGT18200.054.062051
CGCGTGT1529250.053.713561
ATACGGC66850.052.190111
ATCCGGA37100.051.8996
GATCCGG37900.051.5516855
TACGGCG65050.051.2959822
CTCGCGT5800.048.352351
CGGCGAC131350.048.021351
ACCACCG742400.047.6928831
GGCGACC135400.046.339232
TGATACG37850.045.7118722
CCACCGA773150.045.709952
CACCGAG789200.044.8374983
ACGGCGA72600.044.5894933
ACCGAGA804300.044.1798554
CGCTCAT14550.043.7759251
GCGTGTT1883500.043.5076072
TCTCGGA597000.043.14530640-41
CCGAGAT1477650.043.0823941
CGGAGAT578750.042.9779542-43
Produced by FastQC (version 0.11.5)